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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Penelitian Saintek Agrikultura Bionatura Cropsaver : Journal of Plant Protection Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat AGRIVITA, Journal of Agricultural Science Jurnal Agro Jurnal Perlindungan Tanaman Indonesia Kultivasi Jurnal Pengelolaan Laboratorium Pendidikan AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Soil REns Kumawula: Jurnal Pengabdian Kepada Masyarakat International Journal of Environmental, Sustainability, and Social Science Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat
Fitri Widiantini
Departemen Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences

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Journal : HAYATI Journal of Biosciences

 1 
Resistance of Nilaparvata lugens to Fenobucarb and Imidacloprid and Susceptibility to Neem Oil Insecticides Danar Dono; Neneng S Widayani; Safri Ishmayana; Yusup Hidayat; Fitri Widiantini; Ceppy Nasahi
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.234-244

Abstract

This study aims to monitor and determine the level of resistance of N. lugens to fenobucarb and imidacloprid, to analyse of the specific activities of asetylcholinesterase, Glutation S-Transferase, esterase, and to determine sensitivity of resistant N. lugens to the neem oil. Resistance tests were conducted on a field population from Cipunagara, Subang, West Java, Indonesia, and a standard population originating from Banyuwangi, East Java, that was obtained from the Indonesian Center for Rice Research. Synthetic and neem Insecticides were applied using the leaf-stem dipping method. The resistance ratio obtained by comparing the LC50 value of the field population with the standard. The test insects were considered to be resistant to synthetic insecticide if they had a resistance ratio (RR) of more than 4, indicated resistance if RR ≥1, and susceptible to neem if has RR <1. The results of the research showed that the N. lugens population of Cipunagara indicated resistance to fenobucarb (RR = 2.43), while it was clearly resistance to imidacloprid (RR = 13.95). Specific activities of the acetylcholinesterase and Glutathione S-Transferase of the N. lugens Cipunagara populations were higher than the standard, while the esterase specific activity was lower. These two enzymes are responsible for the resistance of N. lugens to fenobucarb and imidacloprid. Nevertheless, the N. lugens population of Cipunagara was susceptible to neem (RR <1) that never been reported before. Therefore, it is important to know the change in the sensitivity of the target and the metabolic pathway of the insecticide used to manage insect resistance.
Metabolites Profiling of Penicillium citrinum Recovered from Endophytic of Ramie (Boehmeria nivea) as a Potential Biocontrol Against Pathogenic Fungi Kartika Sari; Vira Kusuma Dewi; Asri Peni Wulandari; Nia Rossiana; Tati Herlina; Fitri Widiantini
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.246-255

Abstract

Endophytes are a potent source of bioactive compounds that mimic plant-based metabolites. Fungi Fusarium spp. and Clonostachys rosea have been identified as pathogenic microorganisms in ramie (Boehmeria nivea). The antifungal test was carried out using the diffusion method and the MIC50 and Minimum Fungicidal Concentration (MFC) values using the tested microbial pathogens were Fusarium solani isolate 3248941, F. solani isolate Colpat-359, F. oxysporum isolate N-61-2, dan Clonostachys rosea strain B3042. Identification of secondary metabolites of the extract was carried out using GC-MS. The chromatogram of GC-MS analysis of this ethyl acetate extract (EA) showed seven dominant chemical compounds with various biological activities. Ethyl asetate extract of P. citrinum showed inhibition zone ranged from 19.10–22.07 mm with strong-very strong category at 200 mg ml-1 concentration against the tested microbial pathogens. Results revealed that the significant MIC values were observed against F. solani isolate 3248941 and Clonostachys rosea strain B3042 by less than 6.3  mg ml-1 and against F. solani isolate Colpat-359 and F. oxysporum isolate N-61-2 with 12.5 mg ml-1 and 25 mg ml-1 respectively. Minimum Fungicidal concentrations (MFC) for EA of P. citrinum were also reported against F. solani isolate 3248941 and C. rosea strain B3042, by 12.5 mg ml-1, whilst Fusarium solani isolate Colpat-359 by 25 mg ml-1 and Fusarium oxysporum isolate N-61-2 ,by 50 mg ml-1 respectively. Results of this study showed that P. citrinum, endophytic fungi of ramie, could be a promising source of compounds for antifungal agents.
Antagonistic Effects of Bacterial Rhizosphere of Oil Palm in Biocontrol of Basal Stem Rot Disease (Ganoderma boninense Pat.) Widiantini, Fitri; Nugraha, Gema Takbir; Yulia, Endah; Nasahi, Ceppy
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1071-1081

Abstract

Basal stem rot disease caused by Ganoderma boninense is a major problem for oil palm cultivation. The research was conducted to obtain biocontrol agents from rhizosphere of oil palm to control the disease as part of sustainable pest management in oil palm plantation. Rhizosphere bacteria were isolated from rhizospheres of healthy oil palm trees. Isolation of bacteria was done using serial dilution method. The isolated bacteria were then tested for its antifungal activity against G. boninense in vitro using dual culture assay. The ability of the bacteria to produce antifungal compound was also determined by culturing the bacteria on ISP2 liquid media. Once the bacterial cells were removed, the crude metabolites were then tested against G. boninense using agar well diffusion and toothpick colonization. The result showed that several isolates demonstrated strong antifungal activity against G. boninense. Some isolates were also able to degrade chitin and to solubilize phosphate. Furthermore, the crude metabolites produced by the rhizosphere bacteria demonstrated the ability to inhibit the growth of G. boninense in the agar diffusion method. Colonization of the G. boninense on toothpick following soaking in the crude metabolites was also inhibited. The isolated rhizosphere bacteria (BARK7 and BARK15 in which identified as Burkholderia sp.) showed promising ability to be developed as biocontrol agent for basal stem rot disease of oil palm.
Co-Authors Aditya Bintan Pratama Agung Karuniawan Agung Kurniawan Agus Susanto Agus Susanto Agus Susanto Aina Anna Roosda Andang Purnama Andang Purnama Andang Purnama Ania Citraresmini Asri Peni Wulandari Avissa Ayuningdiyas Azis Muslim, Azis Betty Natalie Fitriatin Ceppy Nasahi Citra Bakti, Citra Danar Dono Deni Ariyono Dinda Sekar Ayu Fiko Dwindry Formanda Dwisari Januarily Pitaloka Elga Sari Ema Komalasari Ema Komalasari, Ema Emma Trinurani Sofyan Endah Yulia Endah Yulia Endah Yulia Eso Solihin Fajri Maulana Anugrah Fakhrurroja, Hanif Feliatra Fiko, Dinda Sekar Fitri, Elysa Fuji Hartati Fuji Hartati Ghifari, Raden Faqih Hilmiy Hadi Syahir Muhadam Hersanti - Hersanti HERSANTI Hilda Sandra Utami Ida Nurhelawati Ida Nurhelawati Ifa Dwi LUTHFIANA Kang, Seung Won Kartika Sari Kirana Sonya Harviana Krissanti, Petra Sulistya Dian Lindung Tri Puspasari Luciana Djaya, Luciana Margaretta Christita, Margaretta Mia Rahmah Qadryani Mitalo, Oscar W. Muhammad Maksum Nabilla, Anisa Nadhira, Nadhira Neneng S Widayani Nia Rossiana Nono Carsono Noor Istifadah Nugraha, Gema Takbir Parakkasi, Karmilla Prabowo, Yudhisa Hendri Putra, Fitriansyah Putu Ghita Novilaressa Ramdan Firmansah Rangga Irawan Prasetyo Rika Meliansyah Riswandi, Hafiz Rizqullah, Ahmad Fauzan Safri Ishmayana Santa Monica Santi Sariasih Santika Sari Satriyo Restu Adhi Septiandini, Adelia Serly Noviyawati Silviya Rahma Dhiya Syafira Siska Rasiska SIska Rasiska, SIska Sri Hartati Sri Hartati Sri Hartati Sudarjat Sudarjat Sukmono Suwignyo Susanti, Rista Syahnur, Fitika Syarif Hidayat Tarkus Suganda Tati Herlina Tiara Febricyanti Putri Senjaya Tualar Simarmata Utami, Rahma Dyah Vira Kusuma Dewi Wahyu Daradjat Natawigena, Wahyu Daradjat Wawan Kurniawan Wiwin Widiawati Yunira, Alma Yusup Hidayat Yusup Hidayat