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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Silvikultur Tropika (JST) Hemera Zoa Indonesian Journal of Pharmaceutical Science and Technology Indonesian Journal of Biotechnology Majalah Obat Tradisional JSFK (Jurnal Sains Farmasi & Klinis) ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Jurnal Ilmu dan Teknologi Kayu Tropis Britain International of Exact Sciences Journal (BIoEx Journal) Livestock and Animal Research Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Current Biomedicine Asian Journal of Social and Humanities

IETJE WIENTARSIH

Departement Of Veterinary Clinic Reproduction And Pathology Faculty Of Veterinary Medicine, Bogor Agricultural University, Jl. Raya Dramaga, Bogor, Jawa Barat 16680, Indonesia

Author-ID : 416884

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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PF-18 Photomicrograph of Nanogel Andrographolide-Beta Cyclodextrine Inclusion Complex As Anti-Burns Bayu Febram Prasetyo; Ietje Wientarsih; Dondin Sajuthi; Vetnizah Juniantito
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.194 KB)

Inclusion complex is a complex formed between drug molecules which act as guest or located inside the cavity of host molecule. Host molecules are commonly originated from the derivative group of cyclodextrin. Among cyclodextrin groups, beta cyclodextrin (BCD) is mostly used in formula development and drug delivery system [1].Andrographolide (AG) is a pure isolate chemically syntesized from sambiloto herbs (Andrographis paniculata Nees), in the form of needle cystal-like which is colorless and extremely bitter. AG has variety of medical properties, particularly as anti-inflamation to treat skin burns [2]. However AG has poor solubility in water. This will result in low abillity to solute, penetrate membrane, and distribute the drug when applied transdermally in burn skin. In burn skin, there is tendency to skin damage, especially in stratum corneum which acts as semipermeable barrier. The ability of drugs that applied transdermally tends to be high.Formation of inclusion complex using AG and BCD to increase the ability of AG in penetrating membrane should be done. Transmission Electron Microscope (TEM) is a fast technique to confirm the formation of drug or inclusion complex by comparing the shape and particle size [3]. Study on percutaneous penetration of AG-BCD inclusion complex is produced through solvent evaporation method at mole ration 1:2 in viscolam gel preparation.

Formation of Andrographlolide-BetaCyclodextrin Inclusion to Increase Solubility and Dissolution Rate Bayu Febram Prasetyo; Ietje Wientarsih; Dondhin Sajuthi; Vetnizah Juniantito
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 2 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Andrographolide (AG) is a pure isolate of the chemical synthesis of sambiloto (Andrographis paniculata Nees.) which has various pharmacological properties such as anti-inflammatory and antimicrobial. In order to improve the ability of AG to penetrate the membrane in transdermal use, an inclusion complex was formed using beta-cyclodextrin (BCD) by modifying the physical chemistry properties of AG, particularly the solubility in its base, partition, and distribution on the skin, as well as by changing the permeability of the stratum corneum. The inclusion complexes of AG with BCD were prepared by the solvent evaporation method in the mole ratios of 1:1, 1:2, and 2:1. The solid of the yield of AG inclusion complex in BCD has been tested with a solubility test until it reached equilibrium at 37 ± 5 °C for 24 hours and In vitro dissolution rate test using the II USP method (paddle type), then compared to a single AG compound, and a physical mixture of AG-BCD. Based on the solubility and dissolution rate tests, it showed that the formation of AG-BCD inclusion complex was obtained at the mole ratio of 1:2. Moreover, the AG solubility increased 38 timesand the dissolution rate in the 60th minute increased twice in the inclusion complex with BCD.Key words: andrographolide, inclusion complex, beta cyclodextrin, solubility, dissolution rate

Comparative lactic acid bacteria (LAB) profiles during dadih fermentation with spontaneous and back-slopping methods, as identified by terminal-restriction fragment length polymorphism (T-RFLP) Chandra Utami Wirawati; Mirnawati Bachrum Sudarwanto; Denny Widaya Lukman; Ietje Wientarsih; Eko Agus Srihanto
Indonesian Journal of Biotechnology Vol 26, No 3 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.61164

The diversity of lactic acid bacteria (LAB) present during the manufacture of traditional fermented buffalo milk from West Sumatra, known as dadih, was studied via a culture-independent approach using terminal-restriction fragment length polymorphism (T-RFLP) to compare the dynamic diversity in back-slopping and spontaneous fermentation methods. Total LAB and pH were measured in freshly prepared buffalo milk and in \textit{dadih} fermented for 24 and 48 hours. The results indicated significant differences between the fermentation methods, with higher total LAB, and greater phylotype richness and relative abundance being identified in the back-slopping method. Terminal fragment lengths (TRFs) of 68 and 310 bp were common to both techniques, similar to those of Lactobacillus fermentum, Fructobacillus pseudoficulneus, Leuconostoc citreum, Leuconostoc kimchii, and Leuconostoc sp. The changes in phylotype number (species number) and relative abundances of LAB communities identified are expected to produce data needed to formulate the best fermentation process for dadih manufacturing. A 24-hour back-slopping fermentation method is recommended, as fermentation time of longer than 24 hours reduced viable LAB significantly. Our results also indicated that the T-RFLP technique is not only clearly sensitive enough and adequate for segregating LAB diversity in both fermentation methods, but that it also provides good information regarding the structure of microbial communities and their composition change during the fermentation process.

Biomass and Carbon Mass Allometric Equation Models of Avicennia marina (Forsk.) Vierh. Case Study Pulau Dua Natural Reserve Banten PERSAMAAN ALOMETRIK BIOMASSA DAN MASSA KARBON Avicennia marina (Forsk.) Vierh. Studi Kasus Cagar Alam Pulau Dua Banten Tyas Ayu Lestari; Aswin Rahadian; M. Yanuar J. Purwanto; Ietje Wientarsih
Jurnal Silvikultur Tropika Vol. 7 No. 2 (2016): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.7.2.%p

Research measurements of carbon stocks Avicennia marina has been done by Dharmawan and Siregar (2008) with a DBH 5 cm. Calculation of carbon stocks Avicennia marina with DBH less than 5 cm hadn’t been done. Currently, approaches to measuring biomass and carbon mass mangrove species is being developed through allometric equation. Allometric equation to estimate carbon stocks are still limited, especially for mangrove with DBH less than 5 cm. Therefore, this research carried out calculations to obtain allometric equations for estimating biomass and carbon mass Avicennia marina with DBH less than 5 cm. The results of research can complement allometric equations that have been published previously. Allometric equations constructed using two equations, namely linear and logarithmic with two variables such as total height (x1) and diameter at breast height / DBH (x2). Allometric equation to estimated total biomass, root, stem, branch and leaves are Log YTotal = -7.92 + 2.02 Log (Tt), Log YRoot = -8.37 + 1.94 Log (Tt), Log YStem = -8.83 + 1.99 Log (Tt) + 0.419 Log (DBH), Log YBranch = -8.63 + 2.01 Log (Tt), dan Log YLeaves = -7.73 + 1.63 Log (Tt). Allometric equation to estimated total carbon root, stem, branch and leaves are Log YTotal = -8.82 + 2.21 Log (Tt), Log YRoot = -9.11 + 2.04 Log (Tt), Log YStem = -8.89 +2.06 Log (Tt) + 0.467 Log (DBH), Log YBranch = -9.41 + 2.13 Log (Tt), dan Log YLeaves = -8.46 + 1.64 Log (Tt).Key words: Seedling, stake, total height, diameter at breast height

AKTIVITAS SEDIAAN SALEP EKSTRAK BATANG POHON PISANG AMBON (Musa paradisiaca var sapientum) DALAM PROSES PERSEMBUHAN LUKA PADA MENCIT (Mus musculus albinus) Bayu Febram; Ietje Wientarsih; Bambang Pontjo
Majalah Obat Tradisional Vol 15, No 3 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

The objective of the present research is to study the activity of banana stem extract in ointment solutions in the acceleration of wound healing process on mice skins. Totally of 45 mice strain DDY 4-6 weeks old were devided in negativecontrol group (placebo ointment), positive control group (Betadine® ointment) and Ambon banana stem extract ointment.All mice were aseptically wounded 1-1,5 cm in the anterior region of back skin using a sterile scalpel. The wound wassmeared with the ointment. The pathology anatomy observations was done inday 3, 5, 7, 14 and 21 post wounded. Parameters of the gross lesions (pathology anatomy) observations warecolour of the wound, blood coagulations, scab formations and size of the wound. Parameter for (microscopic lesions) histopathology were infiltrations of inflammatory cells (neutrophils, lymphocytes,macrofages), neo-capillarizations, re-epitelization percentage and the thickness of fibroblast. All quantitative data were measure using ANOVA and continue with Duncan Test, moreover, the qualitative data were presented descriptively. The result shows that gross lesions observations, the extract ointment group was faster in scab formations and covers the wound without trace compared to the negative control group. The statistical test on the infiltrations of inflammatory cells parameter of the extract ointment group significantly different (P<0.05) compared to the negative control group. Histopatologycal observations shows that Ambon banana stem extract in ointment solutions can increase the infiltrations of inflammatory cells, neo-capillary formations, re-epitelizations percentage andacceleration of fibroblast formations. Base on the result the Ambon banana stems extract in ointment solutions can accelerate the wound healing process and it seems that this solution could be developed and uses for the medical purposes.

AKTIVITAS ANTIOKSIDAN FRAKSI METANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) Ietje Wientarsih; Sulistyantie Hr. Sjarif; Irma Maulani Hamzah
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 3, No 2 (2013): FITOFARMAKA
Publisher : Universitas Pakuan

Pegagan (Centella asiatica (L.)Urban) merupakan tanaman herba tahunan yangtumbuh menjalar dan berbunga sepanjang tahun.Pegagan dirujuk sebagaiantiinflamasi, antioksidan, antitumor, antibakteri atau untuk meningkatkan daya ingat(susunan syaraf pusat), eksim, luka bakar dan hepatitis.Tujuan penelitian ini adalahuntuk mengetahui antioksidan fraksi metanol dari ekstrak metanol daun pegagan.Melalui tahapan pemisahan, pemurnian dan menentukan aktivitas antioksidan denganmetode DPPH (1,1-difenil-2-pikril-hidrazil). Pada penelitian ini daun pegagandimaserasi menggunakan pelarut metanol kemudian dilakukan uji fitokimia danidentifikasi gula. Pemurnian dilakukan dengan metode kromatografi kolom denganelusi gradien menggunakan tiga eluen yaitu berturut-turut toluen : etil asetat (1:1), etilasetat, dan metanol. Identifikasi dilakukan menggunakan kromatografi lapis tipis(KLT) siliska gel dengan eluen etil asetat : metanol : air (8,1 : 1,25 : 0,65). Terhadapfraksi metanol dilakukan uji aktivitas antioksidan. Hasil uji fitokimia terhadap fraksimetanol diperoleh golongan senyawa terpenoid, flavonoid, dan alkaloid. Hasil KLTfraksi metanol menunjukkan nilai Rf pada 0,64. Aktivitas antioksidan fraksi metanoldiperoleh nilai IC50sebesar 481,64 ppm.

Stabilitas Fisikokimia dan Sifat Antipenuaan Kulit Formula Krim Berbahan Aktif Alami (Physicochemical stabilities and Skin Antiaging Properties of Bioaktive-based Cream Formulas) Rita K Sari; Nyoman J Wistara; Deded S Nawawi; Nopen Meisaroh; Ietje Wientarsih; D R Agungpriyono; L N Sutardi; Mawar Subangkit; Vetnizah Juniantito
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 15, No 1 (2017): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The purpose of this study was to analyze the physicochemical stabilities and in vivo antiaging properties of the cream formulas of F1, F2, and F3 which contained the active compound of 0.1% 0.18, and 0.36%, respectively. The active compound was the combination of ethyl acetate fraction of Toona sinensis, Centella asiatica, and Acacia mangium leaf (1:2:1). For physicochemical stability tests (color, odor, acidity, and antioxidant activity), the creams were stored for 21 days in the refrigerator, room temperature, and extreme temperature (±40 ⁰C). For in vivo antiaging test, the mice skin were smeared with cream and UV irradiated for 15 minutes per day. After two and four weeks, the mice skins were biopsied. The results showed that the physicochemical properties of creams stored in the refrigerator were stable. The storage of creams at room and extreme temperature decreased the physicochemical properties. The application of cream formulas on mice skin after two to four weeks were able to increase the thickness of epidermis, but has not been able to increase the collagen skin of mice.Keywords: Acacia mangium, Centella asiatica, ethyl acetate fraction of leaf, physicochemical and antiaging properties of cream, Toona sinensis

Edible Bird’s Nest Extract Reduced Expression of Senescence Markers in Bone Marrow Mesenchymal Stem Cells Lina Elfita; Ietje Wientarsih; Dondin Sajuthi; Indra Bachtiar; Huda Shalahudin Darusman
Jurnal Sains Farmasi & Klinis Vol 8, No 1 (2021): J Sains Farm Klin 8(1), April 2021
Publisher : Fakultas Farmasi Universitas Andalas

Edible bird’s nest (EBN) is often consumed as a health food due to its suggested health benefits, including anti-aging effects, however the mechanism is still unknown. This study investigated the effect of EBN extract using long term expansion bone marrow-derived mesenchymal stem cells (BMMSCs) as an aging model. Passage 5 (P5) and passage 8 (P8) BMMSCs were treated with EBN extract, and their proliferation, senescence-associated β-galactosidase (SA-β-Gal) activity, and expression of p16INK4a were analyzed. Treatment of BMMSCs with EBN extract decreased population doubling time (PDT) in P5 but not in P8 BMMSCs. In P5 BMMSCs, 200 ppm EBN extract increased BMMSCs proliferation, with PDT reduced by 27.6%. However, 200 ppm EBN extracts did not affect P8 BMMSCs proliferation, although it increased BMMSCs viability. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract decreased SA-β-Gal activity by 54.8% and 47.1% of the control, respectively (P<0.05). Levels of p16INK4a expression were 5.4-fold lower in P5 BMMSCs treated with 200 ppm EBN extract compared to control (P<0.05). Similarly, treatment of P8 BMMSCs with 200 ppm EBN extract reduced p16INK4a mRNA level by 7.9-fold compared to the control (P<0.05). In order to investigate the pathway of EBN extract inhibition, we further analyzed IL-6 and NF-κB1 expression. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract reduced IL-6 mRNA levels by 7.9-fold and 2.1-fold of control, respectively (P<0.05). We found that 200 ppm EBN extract reduced NF-κB1 mRNA level approximately 2.4-fold both in P5 and P8 BMMSCs (P<0.05). Thus, EBN extract reduces markers of senescence, indicated by decreased SA-β-Gal activity and p16INK4a mRNA level, and this correlated with reduced messenger RNA levels of the pro-inflammatory factor IL-6 and the transcription factor NF-κB1.

Terapi giardiasis penyebab diare non-spesifik pada kucing Rizal Arifin Akbari; Ietje Wientarsih; Bayu Febram Prasetyo; Rini Madyastuti
ARSHI Veterinary Letters Vol. 2 No. 1 (2018): ARSHI Veterinary Letters - Februari 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Kucing jantan berumur 4 tahun bernama Rambo datang ke Klinik Star Vet Ciomas Bogor dengan keluhan menderita diare selama 6 bulan. Kucing memiliki kebiasaan diberikan minum air mentah, sering minum air toilet dan air got. Kucing di diagnosa menderita Giardiasis dengan menggunakan teknik fecal flotation yang ditunjukan dengan adanya kista Giardia sp. sebanyak >30 kista per lapang pandang. Kucing di terapi menggunakan antibiotika metronidazol dosis 20 mg/kg BB, imunomodulator (Echinacea extract) dosis 0.1 ml/kg BB dan Vitamin B12 dosis 0.025 mg/kg BB secara peroral selama 10 hari. Dilakukan pemantauan melalui pengamatan bentuk feses dan menghitung jumlah kista Giardia pada hari ke-0, 5, dan 10 pascaterapi. Pada hari ke-10 pascaterapi, feses sudah mulai berbentuk dan tidak ditemukan kista Giardia sp. sehingga kucing dinyatakan sembuh dari Giardiasis.

Gambaran kristal urin pada hewan model nefrolitiasis hasil induksi etilen glikol Rini Madyastuti Purwono; Ietje Wientarsih; Setyo Widodo; Erni H Purwaningsih; Eva Harlina
ARSHI Veterinary Letters Vol. 3 No. 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Tingginya tingkat keterulangan kasus urolitiasis baik pada hewan maupun manusia, menuntut penelitian dalam mencari solusi menurukan angka keterulangan. Penelitian urolitiasis membutuhkan hewan model urolitiasis yang sesuai dengan kondisi sebenarnya. Salah satu metode yang digunakan adalah menciptakan kondisi hiperoksaluria. Penggunaan induksi etilen glikol menjadi metode yang paling umum digunakan. Induksi ini menghasilkan kristal kalsium oksalat monohidrat.

Co-Authors A.S. Satyaningtijas Agus saputra Ahmad Kurniawan Amrozi Andriyanto . ANGGARA ALDOBRATA Anita Esfandiari April Hari Wardhana April Hari Wardhana April Hari Wardhana Aswin Rahadian Aulia Andi Mustika Bambang Pontjo Priosoeryanto Bambang Purwantara Bayu Febram Bayu Febram Bayu Febram Prasetyo Bayu Febran Prasetyo Chandra Utami Wirawati Chatarina Umbul Wahyuni D R Agungpriyono Deded Sarip Nawawi Deni Noviana Denny Widaya Lukman Dian Firnanda Dodi Darmakusuma Dodi Darmakusumah Dondhin Sajuthi Dondin Sajuthi Dwi Utari Rahmiati Eko Agus Srihanto Erni H Purwaningsih Erni H Purwaningsih Esti Dhamayanti Eva Harlina Farra Sasmita Febryanto, Agung Fikri Hanifah Gunanati Soedjono Gunanti . Hadiyanti, Cresensia Rara Hr. Sjarif, Sulistyantie Huda Shalahudin Darusman I Nyoman Jaya Wistara I Putu Kompiang Ikrar Trisnaning Hardi Utami Iman, Bintang Nurul Indra Bachtiar Indra Bachtiar Irma Maulani Hamzah Istifharany Wahyudina Joko Pamungkas Khoirun Nisa L N Sutardi Lina Elfita Lina Elfita Lina Novianti Sutardi Lina Noviyanti Sutardi Lingga Surya Maret Daulay M. Rafi M. Yanuar J. Purwanto Madyastuti, Rini Maret Daulay, Lingga Surya Maulani Hamzah, Irma Meilisa Lidya Margarita Miranti Fardesiana Putri Mirnawati Sudarwanto MIRNAWATI SUDARWANTO Mokhamad Fakhrul Ulum, Mokhamad Muhammad Bintang Murtiningrum, Fitria Senja Nisa, Nunik Nida Choerun Nopen Meisaroh Nova P. Sulistyawati Nugroho, Radhitya Aryo Nuzul Asmilia Nuzul Asmilia Rahmawati Januar Rahminiwati, Min Rastina R, Rastina Rastina Rastina Rayendra, Raendi Rita Kartika Sari Rizal Arifin Akbari Ros Sumarny Sajuthi, Dondhin Sa’diah, Siti Sebayang, Shalina Setyo Widodo Setyo Widodo SILMI MARIYA Silmi Mariya Subangkit, Mawar Sudarwanto, Mirnawati Bachrum Sulistyantie Hr. Sjarif Sus Derthi Widhyari Tarigan, Ronald TATI NURHAYATI Tika Aryanti Trioso Purnawarman Tyas Ayu Lestari Upik Kesumawati Hadi Vetnizah Juniantito Wiwin Winarsih Yessa, Elma Yuliani

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