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Morfologi Hemipenis Biawak Air (Varanus salvator bivittatus) Mahfud Mahfud; Adi Winarto; Chairun Nisa
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1918.885 KB) | DOI: 10.22146/jsv.29298

Abstract

Varanus salvator bivittatus has a pair of hemipenes, which is macroscopic anatomically like the copulatory organ in other amniotes, cylindrical-shaped (truncus) with quite flexible, and it located on the base of the caudal tailof the cloaca. However, information about the microscopic anatomy of hemipenes of this animal is scientifically unpublicized. Therefore, the aim of this research is to study the microscopic anatomy of hemipenes of male Varanussalvator bivittatus. The animals were sacrificed by exsanguination under deep anesthetized and fixed in 4 % paraformaldehyde through perfusion then observed visceral site and morphometric. Histomorphological evaluationwas obtained by paraffin preparation with section thickness of 3-4 μm then stained in Hematoxylin-Eosin (HE) and Masson’s Trichrome (MT). The results showed that truncus of hemipenes was lined by stratified squamousepithelium and supported with thick of dense connective tissue and contain cavernous body and blood vessels are found, the muscles not found. The presence of connective tissue that supported in the down part sometimesmake hemipenes are rigid while prurient condition. In the caudal of truncus hemipenes there is retractor muscle of hemipenes which arranged by striated muscles. Hemipenes is flexible because contain with much of blood vesselthat found in truncus hemipenes.
Follicular Development of Aged Rats Ovarian Injected Human Umbilical Cord Mesenchymal Stem Cells Resti Rahma Dianti; Alif Iman Fitrianto; Adkhilni Utami; Wining Astini; Adisti Dwijayanti; Frans Dhyanagiri Suyatna; Kelvin Yaprianto; Indra Bachtiar; Aryani Sismin Satyaningtijas; Adi Winarto; Arief Boediono
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 1, JANUARY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i1.6889

Abstract

Female reproductive system showing the fastest signs of aging. The ovarian aging characterized by a decrease in follicular development. Stem cells are undifferentiated cells and can form a variety of different cells as the foundation of tissues and organs. Previous studies reported that Bone Marrow Mesenchymal Stem Cells (BM-MSCs) transplantation can restore follicular development in damaged ovarian rats. This study aimed to analyze the number of follicular development in aged rats and to analyze the capability of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) to improving follicular development in aged rats. This study used 3 mature rats (4 months old), and 9 nine aged rats (22-24 months old), Spraque Dawley (SD) strain. They were divided into four groups. The first and the second group was mature rats and aged rats without injection. The third and the fourth group was aged rats injected hUC-MSCs dose 106 cells/kgBW and hUC-MSCs dose 107 cells/kgBW. The injection carried out 4 times at 3-month intervals. The parameters observed were follicular development and homing image of hUC-MSCs in ovarian tissue. The results showed that the number of follicular developments in aged rats 22-24 months decreased significantly compared to mature rats 4 months old. Injection of hUC-MSCs at dose 106 cells/kgBW and 107 cells/kgBW did not increase follicular development in aged rats. hUC-MSCs did not found in ovarian tissue. It could be concluded that aged rats 22-24 months old no longer productive indicated from the number of follicular developments and corpus luteum decreased. The injection of hUC-MSCs intravenously did not indicate an improvement of follicular development in aged rats 22-24 months old.
Perkembangan Anak Tikus (F1) Asal Induk Penerima Asam Valproat sebagai Model Diabetes Mellitus HADI SUNARYO; WASMEN MANALU; ADI WINARTO; BAMBANG KIRANADI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 2 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.411 KB)

Abstract

To do research and development of antidiabetic drugs, animal models of diabetes in accordance with the conditions is required. The aim of this research was to develop a diabetic model that is fit to the pathophysiology of diabetic condition. The pregnant female rats were divided into 2 groups, one group was orally treated by a single dose of valproic acid (250 mg/kg bw) on day 9th of pregnancy and the other was a control. At the ages of 8, 16 and 24 weeks the blood samples of litters (F1) were taken for glucose, insulin and triglyceride determination. At the same time, pancreatic tissues were collected under deep anesthetic condition for immunohistological study. Results of blood glucose concentrations and histological finding of litters (F1) indicated that at the age of 8 weeks both had showed a similar pattern as compared to control. At the ages of 16 and 24 weeks, blood glucose and insulin level showed a significant increase, while positive insulin cells slightly decreased in number. It can be concluded that treating rat with valproic acid on days 9 of gestation will inhibit pancreatic β cells function. There is an indication that F1 of valproic acid treated pregnant mother start showing a metabolic syndrome at 16 weeks and being pronounce by aging.
Sonometri fetus kambing kacang usia 7 minggu hasil superovulasi menggunakan hormon PMSG Ridi Arif; . Andriyanto; Arief Boediono; Adi Winarto; Fadjar Satrija; Wasmen Manalu
ARSHI Veterinary Letters Vol. 2 No. 1 (2018): ARSHI Veterinary Letters - Februari 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.416 KB) | DOI: 10.29244/avl.2.1.13-14

Abstract

Teknologi superovulasi dapat dimanfaatkan untuk meningkatkan sekresi endogen hormon kebuntingan. Salah satu manfaat dari peningkatan sekresi endogen hormon kebuntingan adalah perbaikan perkembangan lingkungan uterus selama kebuntingan. Kambing Kacang betina sebanyak 8 ekor dan telah dewasa kelamin dengan bobot rataan 22 kg dibagi ke dalam dua kelompok yaitu kelompok kontrol (tidak disuperovulasi) dan kelompok superovulasi menggunakan hormon Pregnant Mare Serum Gonadotropin (PMSG) dengan dosis 15 IU/kgBB. Kambing Kacang percobaan diserentakkan berahinya menggunakan PGF2α sebanyak 2 kali dengan selang 11 hari. Penyuntikan PMSG dilakukan bersamaan dengan penyuntikan PGF2α kedua pada kelompok superovulasi. Setelah berahi, semua kambing dikawinkan secara alami dengan pejantan pilihan. Fetus kemudian diukur secara ultrasonografi (USG) pada usia kebuntingan 7 minggu. Hasil pengukuran menunjukkan kelompok Kambing Kacang hasil superovulasi memiliki ukuran fetus yang lebih panjang daripada kelompok kontrol (P<0.05). Ukuran diameter amnion terpanjang dan terpendek serta tebal dinding uterus terlihat cenderung meningkat pada kelompok superovulasi dibandingkan dengan kelompok kontrol (P>0.05). Kesimpulan penelitian ini adalah superovulasi pada induk Kambing Kacang mampu meningkatkan pertumbuhan fetus sampai dengan usia kebuntingan 7 minggu.
Effect of Intravenous Polyunsaturated Fatty Acids Administration on Gastric Mucosal Integrity in Pig-tailed Macaques with Obstructive Jaundice Dadang Makmun; Dondin Sajuthi; Daldiyono Daldiyono; Adi Winarto; Erni Sulistiawati
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 12, NUMBER 1, April 2011
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (908.415 KB) | DOI: 10.24871/12120118-14

Abstract

Background: Acute gastric mucosal injury commonly occurs in patients with obstructive jaundice. We studied the effect of intravenous polyunsaturated fatty acids (PUFA) administration on gastric mucosal integrity in pig-tailed macaques (Macaca nemestrina) with obstructive jaundice by ligating common bile duct (CBD). Method: The study was conducted between February 2009 and May 2010 at the Primate Research Center, Bogor Agricultural Institute. Eight selected male pig-tailed macaques with 6.625 ± 0.83 kg of body weight were used and divided into two groups. In both groups, laboratory examination, including liver function tests and upper gastrointestinal endoscopy were performed before CBD ligation and every two weeks after ligation. In the first group, intravenous PUFA with the dose of 2 g/day was administered every day since four weeks post-ligation up to four weeks later, and in the second group, intravenous PUFA was administered since before ligation up to eight weeks later. Results: In both groups, increased total bilirubin, direct bilirubin, indirect bilirubin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and gamma glutamyl transpeptidase were obviously found, meanwhile decreased albumin level was detected and the cholinesterase level of both groups remained unchanged. Ulcer formation occurred among the first group during 4 weeks after CBD ligation, and these ulcers showed obvious healing within four weeks after intravenous PUFA administration. In the second group, there was no significant ulcer formation within eight weeks after CBD ligation. Conclusion: The potential appearance of acute gastric mucosal injury which reflected by ulcer formation in pig-tailed macaques with obstructive jaundice was significantly decreased by intravenous PUFA administration. We also have successfully developed animal model of obstructive jaundice by CBD ligation, based on the result of liver function tests. Keywords: acute gastric mucosal injury, intravenous PUFA administration, obstructive jaundice, pig- tailed macaques
PENGARUH EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) TERHADAP GAMBARAN DARAH, AKTIVITAS, DAN FUNGSI KOGNITIF TIKUS Iskandar Mirza; Hadi Riyadi; Ali Khomsan; Sri Anna Marliyati; Evy Damayanthi; Adi Winarto
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.188 KB) | DOI: 10.21157/j.ked.hewan.v7i2.927

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak etanol daun pegagan terhadap gambaran darah dan peningkatan fungsi kognitif pada tikus. Penelitian ini menggunakan 16 ekor tikus Wistar jantan, dibagi secara acak ke dalam 4 kelompok dan masing-masing diberi ekstrak etanol daun pegagan setiap hari dengan level 0, 100, 300, dan 600 mg ekstrak/kg bobot badan selama 8 minggu. Hasil penelitian menunjukkan bahwa pemberian ekstrak pada level 300 mg/kg bobot badan meningkatkan nilai packed cell volume (PCV), sel darah merah (SDM), dan nilai hemoglobin (Hb). Pengamatan aktivitas pada kelompok dengan level 300 dan 600 mg/kg bobot badan menunjukkan adanya peningkatan dibandingkan kelompok kontrol (P0,05). Pemberian ekstrak etanol daun pegagan juga berperan dalam meningkatkan keberhasilan pengenalan lorong target. Hasil di atas mengindikasikan dengan kuat bahwa ekstrak pegagan mempunyai peran penting dalam perbaikan profil hematologi dan meningkatkan fungsi kognitif.
CHANGES IN UTERINE CAPABILITY DUE TO INCREASED LITTER SIZE AT 7 WEEKS OF PREGNANCY IN KACANG GOAT Ridi Arif; Fadjar Satrija; Adi Winarto; Arief Boediono; Wasmen Manalu
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.115 KB) | DOI: 10.21157/j.ked.hewan.v12i4.6558

Abstract

The purpose of this research was to study the changes in uterine support capability power due to increase of litter size in Kacang goat. In this study, 9 pregnant female Kacang goats were divided into 4 different groups based on litter size, namely 1, 2, 3, and 4. At 7 weeks of pregnancy, the experimental Kacang goats were sacrificed to observe the macro and micro parameters of the uterus. The results showed that the litter size had a quadratic relationship with micro parameters of uterus but it had a linear relationship with macro parameters of the uterus. The variables of uterine glands area, glands lumen area, and cytoplasmic area reached maximum condition at the litter size of 2.5. Litter size had a linear relationship with the volume, weight, and dimension of the uterus. It can be concluded that the optimal number of litter size in Kacang goat was two offsprings which was proven by the optimal function of the uterus to support fetal development.
PROLIFERASI DAN DIFERENSIASI SEL TULANG TIKUS DALAM MEDIUM KULTUR IN VITRO YANG MENGANDUNG EKSTRAK BATANG Cissus quadrangula Salisb. (SIPATAH-PATAH) Ita Djuwita; Irma Amalia Pratiwi; Adi Winarto; Mustafa Sabri
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.789 KB) | DOI: 10.21157/j.ked.hewan.v6i2.295

Abstract

Penelitian mengenai pengaruh ekstrak batang Cissus quadrangula Salisb. (sipatah-patah) terhadap tingkat proliferasi dan diferensiasi sel-sel tulang tikus (Sprague Dawley) prepuber umur empat minggu menggunakan sistem kultur in vitro. Sel-sel tulang dikultur dalam medium dulbecco’s modified eagle’s medium (DMEM) yang diberi tambahan newborn calf serum (NBCS) 10%, non essential amino acid (NEAA) 10%, NaHCO3, ITS 1 µl/ml (mengandung insulin 5 μg/ml, transferin 10 μg/ml, selenium 5 μg/ml; Sigma St Louis USA), dan 50 µg/ml gentamisin (mDMEM), dengan dan tanpa ekstrak Cissus quadrangula (CQ). Penelitian terdiri atas lima perlakuan yakni kontrol positif (mDMEM + deksametason 10-8 M), kontrol negatif (mDMEM), dan tiga konsentrasi CQ: mDMEM + CQ 0,3 mg/ml; mDMEM + CQ 0,6 mg/ml; dan mDMEM + CQ 1,2 mg/ml. Kultur dilakukan dalam inkubator CO2 5%, pada suhu 37° C selama tujuh hari. Parameter yang diamati adalah konsentrasi sel, komposisi, diameter osteoblas, dan diameter osteosit. Konsentrasi sel dihitung menggunakan hemositometer Newbauer. Komposisi osteoblas dan osteosit ditentukan berdasarkan pengamatan morfologi di bawah mikroskop cahaya. Diameter sel diukur menggunakan eyepiece micrometer. Data dianalisis menggunakan analisis varians dan uji Duncan. Hasil penelitian menunjukkan bahwa pemberian ekstrak Cissus quadrangula Salisb. pada konsentrasi 0,3 mg/ml; 0,6 mg/ml; dan 1,2 mg/ml secara signifikan dapat meningkatkan proliferasi sel tulang; dan pada konsentrasi 0,6 mg/ml mampu menginduksi diferensiasi osteoblas menjadi osteosit (P0,05). Disimpulkan bahwa pemberian ekstrak Cissus quadrangula pada konsentrasi 0,6 mg/ml ke dalam medium kultur dapat meningkatkan proliferasi dan diferensiasi osteoblas.
THE PROFILE OF SUPEROXIDA DISMUTASE AND MALONDIALDEYDE LEVEL IN THE LIVER TISSUE OF HYPERCHOLESTEROLEMIC RATS TREATED WITH Holothuria nobilis POLYSACCHARIDE Fitrah Asma Ulhusna; Adi Winarto; Tutik Wresdiyati
Jurnal Kedokteran Hewan Vol 13, No 2 (2019): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (525.773 KB) | DOI: 10.21157/j.ked.hewan.v13i2.13189

Abstract

The aim of this research was to analyze the profile of superoxide dismutase (SOD) and malondialdehyde (MDA) on the liver tissue of hypercholesterolemic rats which were given Holothuria nobilis polysaccharides (HNP). A total of 15 male rats strain Sprague Dawley were divided into prevention and curative groups. Prevention group consisted of negative/non-hypercholesterolemic group (K-), positive/hypercholesterolemic group (K+), and hypercholesterolemic prevention group which were given 1% cholesterol diet and HNP at dose of 400 mg/kg bw (PCh). The treatments were given for 28 days. The curative group was consisted of the hypercholesterolemic group, which was given 1% cholesterol diet for 28 days, then followed by standard diet for 28 days (Ch), and the hypercholesterolemia curative group which was given 1% cholesterol diet for 28 days, then followed by 400 mg/kg bw HNP for 28 days (ChP). The antioxidant activity of HNP was analyzed by DPPH method. At the end of study the liver tissue was collected and analyzed for MDA, SOD while Cu,Zn-SOD was analyzed by immunohistochemical technique. The results showed that the antioxidant activity of HNP was weak. The MDA level (µg/g) in K-, K+, PCh, Ch, and ChP groups were 1.19±0.6; 3.37±0.79; 0.29±0.14; 9.11±0.72; and 3.14±1.06, respectively. The SOD activities (U/g) in K-, K+, PCh, Ch, and ChP groups were 2141.11±83.88; 1541±211.69; 2096.67±166.66; 1063.33±88.19; 1685.55±167.77, respectively. The immuno reactivity of Cu,Zn-SOD showed that HNP could increase Cu,Zn-SOD in the liver tissues of both groups. This study concluded that the HNP increased SOD activity, Cu,Zn-SOD antioxidant content, and decreased MDA levels in the liver tissues of hypercholesterolemic rats in both preventive and curative groups.
MIKROMORFOLOGI ALAT KELAMIN PRIMER BIAWAK AIR (Varanus salvator bivittatus) JANTAN (Micromorphological Structure of Primary Reproductive Organ of Male Water Monitor Lizard (Varanus salvator bivittatus)) Mahfud M; Adi Winarto; Chairun Nisa
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.061 KB) | DOI: 10.21157/j.ked.hewan.v10i1.3375

Abstract

The study aims to determine the histological structure of the testes, ductus epididymidis and ductus deferens of Varanus salvator bivittatus. The tissue samples were obtained by the histological techniques preparation with thickness section is 3-4 µm then stained using hematoxylin-eosin (HE) and Masson's Trichrome (MT). The result showed that testes are covered by tunica vaginalis and tunica albuginea (tunica fibrous and tunica vasculosa). Tubulus seminiferous as a parenchyma are found in lobules of testes which are formed by septum as extension of tunica albuginea. Tubulus seminiferous is composed by layers of germinal epithelium cells consist of spermatogenia, Sertoli cell, spermatocyte and spermatid which is developed further to be spermatozoa in the lumen of tubulus. Among the tubulus seminiferous, there are groups of epithelioid cells called Leydig cells. The end of tubulus seminiferous formed ductus epididymidis which then ended up and known as ductus deferens. Epididymis is covered by dense connective tissue. Ductus epididymidis is divided into three segments: cranial, medial and caudal. It is lined by pseudostratified columnar epithelium which is varied in its thickness, amount and diameter of lumen. Lumen of the ductus deferens was lined by pseudostratified ciliated columnar epithelium and thin muscularis mucosal layer and thick circular smooth muscle were covered externally.Key words: Varanus salvator bivittatus, testes, ductus epididymidis, ductus deferens, micromorphology