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Agus Santosa
Universitas Muhammadiyah Purwokerto
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Narra J

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Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection Masyeni, Sri; Kuntaman, Kuntaman; Aryati, Aryati; Sofro, Muchlis AU; Hadi, Usman; Mastutik, Gondo; Purnomo, Windu; Santosa, Agus; Yohan, Benediktus; Nelwan, Erni Juwita; Sasmono, R. Tedjo
Narra J Vol. 1 No. 1 (2021): April 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i1.31

Abstract

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.
The role of mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) on cytokine production during dengue virus infection Masyeni, Sri; Kuntaman, Kuntaman; Aryati , Aryati; Sofro, Muchlis AU.; Hadi, Usman; Mastutik, Gondo; Purnomo, Windu; Santosa, Agus; Iqhrammullah, Muhammad; Yohan, Benediktus; Nelwan, Erni J.; Sasmono, R. Tedjo
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.167

Abstract

Inability to understand the pathogenesis of severe dengue, in particular the control mechanism of immune responses, has led to high mortality rate for patients with dengue shock syndrome (DSS). The aim of this study was to determine the control mechanism of cytokine production by mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) during DENV infection. Peripheral blood mononuclear blood cells (PBMC), isolated from healthy individuals, were infected with dengue virus (DENV)-2 strain SJN-006 Cosmopolitan genotype (isolated from Bali, Indonesia). The relative gene expression of SOCS-3, TLR-3, NFκB, and the cytokine genes (interleukin (IL)-6, IL-8, interferon inducible protein 10 (IP-10), and macrophage inflammatory protein-1 beta (MIP-1β)) were measured using qRT-PCR at 6, 12 and 24 hours post infection (hpi). Student t-test and Mann-Whitney test were used to compare the gene expressions while causal correlations were analyzed using regression test and path analyses. DENV-2 infection increased the gene expression of SOCS-3, TLR-3, and NFκB after 12 and 24 hpi. The expression of IL-6, IL-8, IP-10, and MIP-1β genes was increased and peaked at different times post-infection. NFκB and SOCS-3 genes likely have role in the upregulation of IL-8 and IL-6 gene expression, respectively. MIP-1β gene expression was significantly induced by both NFκB and SOCS-3. In conclusion, our study suggested that SOCS-3, TLR-3, and NFκB are important in regulating the production of IL-6, IL-8, IP-10, MIP-1β during early phase of DENV-2 infection. This enriches our understanding on pathogenesis pathway of DENV-associated cytokine storm.
Co-Authors Abdul Rahman Abdurrahman Rahim Thaha AGUS PRIYANTO Agus Priyanto Akhmad Solihin Akmal Nurjamil Ali Budiman Ali Djamhuri Andre Irwanto Andry Listiana Kurniawati, Andry Listiana Andy Supriyadi Annisa Azzahra, Annisa Ariska, Dwi Kuat Ariska, Dwi Kuat Aryati , Aryati Aryati Aryati Asmiati, Dewi Ayunitias, Imelda Azka Raga, Ridha Budiman, Ali Cherly Kemala Ulfa Damanik, Joyakin Dedi Kurnia Dedi Supriadi Destiana, Destiana Devinta, Made Ratih Santi Dewi, Prima Dian Herdiana Diari, Padma Diky Hadiansyah Eka Vivtin Agustiani Endiyono . Endiyono, Endiyono Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Fachry Abda El Rahman Fadilla, Sarah Fajar Suryo Antoro, Aji Fajri, Rizqi Nur Falah Ali Aiman Fariz Maulana Febriyanti, Ika Putri Firdaus, Muhammad Aulia Iqbal Gita Febria Friskawati Gita Febriafriskawati Gondo Mastutik Gumelar, Nanda Cikal Hadyansah , Diky Hamzah, Hasyrul Hana Nabila Heru Sulistiadinata Heru Sulistyadinata Husnul Khotimah Ihda Maulida Nihayatun Nikmah, Ihda Maulida Indra Solehudin Iqhrammullah, Muhammad Irwanto, Andre Jasrial Jasrial, Jasrial Jebul Suroso Jufri Yandes Juliana, Ari Juwita, Dewa Ayu Putu Ratna Kuncoro, Setyo Kuntaman Kuntaman Kurnia, Dedi Kusumadhani, Elvira Isza Laksmi, Putu Pradnyasanti Linda Yanti Listiono, Dwi Listiono, Dwi Lutfi Chabib, Lutfi MADE RATNA SARASWATI . Mahardika, Wayan Gede Masyeni, Sri Muslim, M. Rifqi Fadillah Muslimah, Rukhul Hayati Mustiah Yulistiani muzaenah, tina Neneng Khasanah Nugroho, Yopi Aji Nur Isnaini, Nur Nur Wening, Nur Okta Fajar Silviana Pradnyawati, Luh Gede Purnomo, Windu Putri Pertiwi, Husna Rahayu, Pujen Tri Rahmaningsih, Widi Rama Adha Septiana Ramadani, Krisma Novi Ramdani, Meida Laely Ratri, Ritma Rifan Setiawan Rooseno, Ratna Rayeni Natasha rusmono, widi Rusmono, Widi Sangkala Sasmono, R. Tedjo Savitri, Vira Nur Septiana, Rama Adha Setiyaningsih, Rani Setyorini, Herin Siti Nuraeni, Siti Sofiyawati, Estri Sofro, Muchlis AU Sofro, Muchlis AU. Soleha, Rama Nanda Sri Suparti Sulton Ahmad Supriady, Andi Supriyadi Supriyadi SURTININGSIH, SURTININGSIH Susilawati, Oting Syarif Fadillah Tri Gunarsih Usman Hadi Utami, Evi Dwi Widari, DAP Niti Yohan, Benediktus Yuni Kurniasih, Yuni Zulfa, Aqilatun