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BIOACTIVE METABOLITES OF Lactiplantibacillus plantarum ISOLATED FROM Sonchus arvensis AS AN ANTIMICROBIAL AGENT Syaputri, Yolani; Nurhidayat, Fahmi; Wulandari, Indri; Rahayu, Sri Rejeki
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.6488

Abstract

Sonchus arvensis is a plant widely found in Southeast Asia and often used in traditional medicine. Lactiplantibacillus plantarum is a bacteria isolated from S. arvensis and categorized as qualified presumption of safety by the European Food Safety Authority and the US Food and Drug Administration. Therefore, this study aimed to determine the bioactive metabolites of Lpb. plantarum as antimicrobial agents. Lactic acid bacteria (LAB) were isolated using the serial dilution method, followed by isolation and amplification of the DNA through a 16S rRNA universal primer. Antimicrobial activity was screened using the well-diffusion method. Plantaricin gene identification was performed using PCR and the determination of lactic acid content was conducted using Spectrophotometric. In addition, the titration method was used to measure and determine the hydrogen peroxide. The results showed that Lpb. plantarum had higher inhibition toward pathogen bacteria than Lc. lactis. Lpb. plantarum had the largest inhibition zone against B. subtillis, followed by S. aureus and E. coli, respectively. It was discovered that Lpb. plantarum precipitated with ammonium sulfate had a greater protein content and antibacterial activity. Furthermore, Lpb. plantarum, which encodes plnA and plnEF, produced lactic acid and hydrogen peroxide at concentrations of 3.0158±0.2774 mg/mL and 0.195±0.04 mg/mL, respectively.
Studi Etnografi Suku Baduy: Pengembangan Produk Lokal dan Olahraga Tradisional Terintegrasi Android dalam Meningkatkan Ekonomi Masyarakat Baduy dan Motivasi Olahraga Sari, Nugrahani Febrina; Ariestika, Elsa; Amni, Hazrina; Wulandari, Indri
Jurnal Sporta Saintika Vol 10 No 2 (2025): Jurnal Sporta Saintika Edisi September 2025
Publisher : Departemen Kesehatan Dan Rekreasi Fakultas Ilmu Keolahragaan Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/sporta.v10i2.471

Abstract

Changes in time and development technology have impact positive. Application android based can become great opportunity​ in maintain existence something region Wrong the only one that is public ethnic group Baduy. 2024 Tourists​ as many as 40,153 people visited to region ethnic group Baduy. Purpose study this that is marketing product local people in the Baduy tribe​ through android applications that can accessible. Method research used​ that is study and development Where adoption model development using a waterfall model consisting of from stages analysis need, design system, implementation, and testing stage​ testing this androidapps rated by IT experts use test testing box black, and done test try field through respond traveler and respond public local customs Baduy. Results analysis use questionnaire scale linkert and respond determine level Agreement to something statement with choose available options​ with slaka 1-5. Results percentage by 86.5% through criteria interpretation score of the 30 respondents included in category very Good and product the worthy used. Application with title namely " Baduy Heritage & Traditional Sports" can help give information traveler for visit to Baduy Good from history, UMKM products local Baduy, until get motivation sport for do sport traditional.
Co-Authors Afifah, Shelvira Azka agus, apri Aldo Naza Putra Almina Selian, Rada AMELIA, MITHA Amira, Suci Andika Prabowo Anggraini, Oktavia Ariani, Tya Ariestika, Elsa Atradinal Aulia, Hilsa Azzahra, Adisa Syafa Badri, Ulfa Bakhrun, Akhmad Barnanda Rizky, Oddie Basagevan, Rahma Maulia Fadli Budiawati SUpangkat Iskandar, Budiawati SUpangkat Candana, Dori Mittra Candrawati, Triesti Darnia, Meriza Elpha Dede Mulyanto Deden Nurjaman, Deden Dhafina, Ghayast Naurah Edmizal, Eval Elfiswandi, Elfiswandi Erri Noviar Megantara Fadhliyah Malik Fahmelia, Diva Febrianto, Puput Febriola, Wella Fianti, Nafa Destri Fitri, Elva Rahmi Frizki Amra Gemilang Lara Utama Hazrina Amni Herri Y. Hadikusumah, Herri Y. Hilman, Seprian Hindun Mila Hudzaifah Irnawati Irnawati Jabbar, Asriullah Jannah Rizal, Wadratul Jauhan, Jirjiz Jibrael Rorong, Michael Jufri, Dwi Arini Khairunnisa, Nabilah Fathimah Laila Laila, Laila Liqo, Taqwa Syahidul Mubarak Mubarak Munir, Ali Mutiara Halida, Erda Nasrudin Nur Hikmah Nurhidayat, Fahmi Oekan Soekotjo Abdoellah Okwani, Yustian Parikesit Parikesit Permadi, Andes Prasetya, Rizky Prasetyaningtyas, Raissa Maharani Pujianto, Muhammad Pahla Putri Cahyani, Dwi Rafiannisa, Kinanti Rahayu, Sri Rejeki Ramadhan, Syadza Ghaida Ria Mariani Rossa, Fayola Rusdi, Anisa Safira, Nadya Aulia Samori, Dwiena Anggrekdhita Egidya Sari, Nugrahani Febrina Septian Raibowo Setyawan Widyarto Shanida, Sya Sya Sumaryanti Sumaryanti Syafitri, Miftahul J. F. Syahputra, M. Fadila Syaputri, Yolani Syuhada, M. Abdil Syihab Tanra, Najwa Audina Taufia, Dina Teguh Husodo Ulfa Ulfa wahyuni wahyuni Wibowo, Diki Prayugo Wibowo, Tri Setia Widodo, Fauzi H. P. Wirasutisna, Komar R Y, Muhammad Ilyas Yusep Suparman Yustinus Sukarmin Zarya, Fiky Zhafira, Aulia