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All Journal Jurnal Kebidanan Midwiferia Jurnal Ipteks Terapan JIK- JURNAL ILMU KESEHATAN Jurnal Bidan Cerdas Human Care Journal JURNAL MUTIARA NERS Real in Nursing Journal Jurnal Pembangunan Nagari Maternal Child Health Care Journal Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Menara Medika JURNAL INOVASI KESEHTAN MASYARAKAT pendidikan, science, teknologi, dan ekonomi Jurnal Pengabdian Masyarakat Putri Hijau Jurnal Endurance: Kajian Ilmiah Problema Kesehatan Edunity: Kajian Ilmu Sosial dan Pendidikan Riset Informasi Kesehatan Al Makki Health Informatics Journal Journal of Health Science and Medical Therapy JOURNAL OF COMMUNITY SERVICE AND APPLICATION SCIENCE (JCSAS) Jurnal Pustaka Cendekia Hukum dan Ilmu Sosial Bulletin of Inspiring Developments and Achievements in Midwifery Jurnal Kesehatan
Evi Hasnita
Fakultas Kesehatan Universitas Fort De Kock
Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Comparison of DNA Extraction Feasibility from Menstrual Blood and Endometrial Tissue in Reproductive-Aged Women Febriyeni; Hestiantoro, Andon; Asmarinah; Tulandi, Togas; Hasnita, Evi; Zuraida; Harnaldo Putra, Billy; Octariyana; Rizki Amanda, Clara; Astria Rishel, RIka
Jurnal Kesehatan - STIKes Prima Nusantara Vol 16 No 2 (2025): Jurnal Kesehatan Volume 16 Nomor 2 Tahun 2025
Publisher : LPPM Universitas Prima Nusantara Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35730/jk.v16i2.1314

Abstract

ABSTRACT The endometrium is a highly vascularized tissue composed of numerous blood vessels, with cells that are formed and destroyed during each menstrual cycle. Menstrual blood, which is shed cyclically from the endometrium in women of reproductive age, contains endometrial tissue or cells. Both deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are the primary nucleic acids present in cells with nuclei. In this study, 20 samples of menstrual blood and endometrial tissue were collected from women of reproductive age. Menstrual blood was gathered using specially designed filter paper, and DNA was extracted using the QIAamp DNA Mini Kit (Qiagen, Cat No.: 51304). Endometrial tissue was obtained via biopsy, and DNA was extracted using the Geneaid DNA Isolation Kit (Geneaid, New Taipei, Taiwan). DNA purity and concentration were measured using a Thermo Scientific Nano-Drop microvolume Spectrophotometer. The results indicated an average DNA purity of 1.88 ± 0.09 and a mean concentration of 116.9 ng/µL for menstrual blood, while endometrial tissue had a median DNA purity of 1.92 and a concentration of 192 ng/µL. Statistical analysis revealed no significant difference in DNA purity between menstrual blood and endometrial tissue (p = 0.083), but a significant difference in DNA concentration was observed (p = 0.002). Although there was a slight difference in DNA concentration, both menstrual blood and endometrial tissue samples were equally effective in preserving optimal purity and concentration of DNA molecules. Keywords: Deoxyribonucleic Acid, DNA, Endometrial Tissue, Menstrual Blood, Nucleic Acid.
Combined Iron Tablets and Ambon Banana Improve Hemoglobin in Anemic Second Trimester Pregnancy Herawati, Mita; Hasnita, Evi; Iswandari, Hargianti Dini
Jurnal Kebidanan Midwiferia Vol. 12 No. 1 (2026): April
Publisher : Universitas Muhammadiyah Sidoarjo

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Abstract

Anemia during the second trimester of pregnancy poses significant health risks to both the mother and the fetus. Food-based strategies combined with iron supplementation may improve hemoglobin response in this critical period. This study aimed to analyze the effect of combining Fe tablets and Ambon banana consumption on hemoglobin levels among second-trimester pregnant women with anemia compared to Fe supplementation alone. A quasi-experimental pretest-posttest control group design was employed. A total of 54 anemic pregnant women in their second trimester from three public health centers in Pondok Kelapa District, Central Bengkulu, were evenly divided into two groups of 27 participants each. The intervention group received one Fe tablet daily and one Ambon banana twice daily for 30 days, while the control group received one Fe tablet daily. Hemoglobin levels were measured before and after intervention using an Easy Touch GCHb Hemoglobin Meter. Both groups showed increased hemoglobin after the intervention. The post-intervention mean hemoglobin level was 11.952 ± 0.6941 g/dL and 11.093 ± 0.6108 g/dL in the intervention and control groups, respectively, with a between-group difference of 0.8593 g/dL (p < 0.001). Confidence intervals could not be reported because summary data lacked the standard error or standard deviation of the change score. Fe tablets combined with Ambon banana consumption were associated with a greater hemoglobin increase than Fe tablets alone among anemic second-trimester pregnant women.
Co-Authors Adriani Mahdarlis Adriani Suwito Afriwardi Yusri Andon Hestiantoro Aria Wahyuni Arina widya Asmarinah Astria Rishel, RIka Billy Harnaldo Putra, Dini Fatmi, Chantia Octazuria Cici Aprilliani Despa Wildawati Dewi Susanti Efriza Elisabeth Dame Manalu Emilda, Emilda eni yuliawati Erit Rovendra Faridah Mohd Said Febrina, Cory Febriyeni Fitriani, Oriza Sativa Fury Indah Girsang, Rostiodertina Hardisman Hargianti Dini Iswandari Herawati, Mita Hidayati, Tri Riska ihsan kurnia Ikhwana Elfitri Iswahyudi, Abdi Juliandi Jeri Lazdia, Wenny Legabina Adzkia Lismawati Manaluu, Elisabet Miming Andika Mudjiran muhammad effendy Muhammad Irfan Nadia, Sri Nambiar , Nisha Nanda Nanda Nanda Novradayanti Neila Sulung Neila Sulung, Neila Nisha Nambiar Noflidaputri, Resty Novi Wulan Sari Nurdin Nurdin Nurhayati Nurhayati Nurhayati Nurhayati Nurhayati Octariyana Oktavianis Oktavianis Pratiwi, Endang Dian Putra, Fajrian Aulia Putri Nilam Sari Putri Rahmadani, Putri Rahmadasmi, Nola Rahmat Saputra Rahmi Sari Kasoema Ramadanti, Tika Ratna Dewi Ratna Dewi Rina Mariyana Rina Mariyana Rizanda Machmud Rizki Amanda, Clara Rizqa Hasanah Rostiodertina Girsang Said, Faridah Mohd Sari, Novi Wulan Savitri Yolanda Sherly Gusvianti Silvia Silvia Silvia Suradi sri permatadewi Susanti, Nadia Syafriyanti, Mega Tri Riska Hidayati Trinanda Wahyuni Tulandi, Togas Usman, Yeni Wartsa Wati, Del Fatma Wenny Lazdia Wenny Lazdia Wenny Lazdia Wiwit Febrina Yantri Maputra Yelmi Reni Putri Yosta Defina Yuniliza, Yuniliza Yusrawati Yusrawati Zuraida Zuranda