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TRAINING OF TRAINER (TOT) DALAM UPAYA PEMELIHARAAN KESEHATAN GIGI UNTUK ORANG TUA DAN SISWA DI SD RADEN PAKU SURABAYA Rahmitasari, Fitria; Rosita Irmawati, Ari; Revianti, Syamsulina; Nilawati, Nina; Widyastuti; Kurniawan, Hansen; Bayu Prabowo, Puguh; Pangabdian, Fani; Nur Ashrin, Meinar; Widaningsih; Handayani, Budi; Khoironi, Emy; Lukisari, Cane; Samadi, Karlina; Harnowo, Setyo
Jurnal Pengabdian Masyarakat Pesisir VOLUME 3 NOMOR 2
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v3i2.120

Angka kejadian permasalahan kesehatan gigi dan mulut di Indonesia mencapai 57,6%, dimana tergolong sangat tinggi, dengan 93% di antaranya dialami oleh anak-anak usia sekolah. Pengetahuan, sikap, dan perilaku individu memiliki peran yang penting dalam menjaga kesehatan gigi mulut. Lingkungan sekitar anak, terutama keluarga dan guru, memiliki peran yang krusial dalam membentuk sikap dan perilaku mereka. School Health Program (SHP) yang melibatkan pelatihan Training of Trainer (ToT) mengenai kesehatan gigi mulut bagi guru dan orang tua mencakup kegiatan menyikat gigi dua kali sehari selama 21 hari. Program ini bertujuan untuk memberikan pendidikan kepada anak dan orang tua beserta guru tentang perlunya menjaga oral hygiene yang baik serta mengajarkan teknik menggosok gigi dengan pasta gigi berfluoride. Inisiatif ini juga mendukung pencapaian target "Indonesia Bebas Karies 2030" dan dilaksanakan secara daring melalui aplikasi Zoom. Pemahaman peserta terkait kesehatan gigi dievaluasi melalui kuesioner sebelum dan sesudah diberikan pelatihan. Analisis statistik menggunakan uji t-sampel berpasangan untuk mengetahui dan membandingkan nilai rerata sebelum dan sesudah diberikan pelatihan menunjukkan nilai p<0,05, yang mengindikasikan adanya peningkatan pengetahuan yang signifikan mengenai pemeliharaan kesehatan gigi mulut pada guru serta orang tua siswa di SD Raden Paku melalui program ToT. Kegiatan ini terbukti efektif dalam meningkatkan pengetahuan dan perilaku mengenai kesehatan gigi dan mulut setelah materi diberikan.

The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Rahmitasari, Fitria; Cevanti, Twi Agnita; Prabowo, Puguh Bayu; Sularsih, Sularsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups

Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Zefanya, Kartika; Rahmitasari, Fitria; Widaningsih, Widaningsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.

Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Zefanya, Kartika; Rahmitasari, Fitria; Widaningsih, Widaningsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.

The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Rahmitasari, Fitria; Cevanti, Twi Agnita; Prabowo, Puguh Bayu; Sularsih, Sularsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups

Tegillarca granosa shell combination with Vitis vinifera and fluoride in decreasing enamel microporosity Setiawan, Grace Caroline; Tinisia, Adelia; Rahim, Muhammad Galang Adhinata Abdul; Rahmitasari, Fitria; Prananingrum, Widyasri
Dental Journal (Majalah Kedokteran Gigi) Vol. 57 No. 2 (2024): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i2.p131-138

Background: White spot lesion is a demineralization process indicated by the increased of enamel microporosity. A tegillarca granosa shell contains 98.7% calcium and Vitis vinifera contains phytochemical compounds with fluoride, which has a potential to stimulate remineralization. Purpose: To analyze the Tegillarca granosa shell combination with Vitis vinifera and fluoride in decreasing enamel microporosity. Methods: The cream was prepared by combining 10% and 20% Tegillarca granosa shell with 10 grams of Vitis vinifera extract and 100 mg of fluoride. The cream was tested beforehand for viscocity and pH. Furthermore, 16 premolars were etched and divided into four groups. Group 1 was smeared with placebo (negative control) and Group 2 was smeared with casein phosphopeptide-amorphous calcium phosphate (positive control). The other groups were smeared with cream 10% (Group 3) and 20% (Group 4) Tegillarca granosa shell combination with Vitis vinifera and fluoride. Teeth were treated three times a day for 30 minutes and soaked in artificial saliva. After 14 days, the enamel microporosity was carried out using a scanning electron microscope. The data was analyzed with one-way analysis of variance (ANOVA) test followed by post-hoc least significant difference (LSD). Results: The enamel microporosity showed significant difference between Group 1 and the other groups. There was no significant difference between Groups 2, 3, and 4 (p<0.05). Although there was no significant difference between Group 3 and 4, the lowest one was in Group 4 (p>0.05). Conclusion: The cream, prepared by combining Tegillarca granosa shell with Vitis vinifera and fluoride, is effective in decreasing the enamel microporosity.

The effect of effervescent acanthus ilicifolius l. extract on antifungal properties and flexural strength acrylic Prananingrum, Widyasri; Rahmitasari, Fitria; Andriani, Dwi; Razak, Arifzan
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Acanthus ilicifolius L. has antifungal potential and makes it possible to be a candidate for denture cleanser. This study aims to evaluate the effect of effervescent Acanthus ilicifolius L. extract on antifungal properties and flexural strength acrylic after immersion for three duration times.Method: Extracts were made by maceration method with ethanol. For the antifungal test, effervescent Acanthus ilicifolius L extract was prepared by combining the Acanthus ilicifolius L extract 4% and sodium perborate with two ratios, 1:7 and 1:14. The microdilution method was carried out. Effervescent Acanthus ilicifolius L extract 4% with ratio 1:7 was prepared for the flexural strength test. The fifty-four heat-cured acrylic resin (20x10x2.5mm) were fabricated and divided into 3 groups. Group 1 was immersed in aquadest (control); Group 2 was immersed in sodium perborate; Group 3 was immersed in effervescent Acanthous ilicifolius L. extract. Each group was immersed for 15 min, 8, and 56 hours (n=6). The flexural strength test was performed using Universal Testing Machine.Result: Effervescent Acanthus ilicifolius L. extract exhibited antifungal activity which increased in an effervescent Acanthus ilicifolius L extract-dependent manner significantly (0.57 to 0.50 OD). There were no significant differences between sodium perborate, effervescent_AI1 (1:7) and effervescent_AI2 (1:14). The flexural strength was found to be within the range of 66.1 - 68.3 MPa. There were no significant differences between groups (p < 0.05).Conclusion: Effervescent Acanthus ilicifolius L extract with the ratios 1:7 had a good antifungal effect and appropriate flexural strength after immersion for 15 min, 8, and 56 hours.

Shear bond strength fissure sealant based on glass ionomer after topical fluor application: a comparison between sodium fluoride and acidulated phosphate fluoride Akwan, Yulie Emilda; Paramita, Annisa Listya; Rahmitasari, Fitria
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Application of fissure sealants to young permanent teeth is one of the preventive measures for caries in the field of dentistry. Fissure sealants based on glass ionomer cement (GIC) have the advantage of releasing fluoride. Sodium fluoride and acidulated phosphate fluoride (APF) are remineralization agents because they can increase remineralization by altering hydroxyapatite to fluorapatite and this in turn makes fluorine able to increase pH. Fluoride can prevent caries by increasing remineralization of the enamel that has just been demineralized by acids produced by plaque bacteria. The aim of this study is to compare the shear bond strength fissure sealant based on glass ionomer between sodium fluoride and APF topical fluor applicationMethod: This research applied true experimental design with post test only control group design. The 21 maxillary permanent premolars were distributed into 3 groups, namely the control group (K1), the treatment group added 5% sodium fluoride (K2), and the treatment group added 1.23% APF (K3). Result: One Way ANOVA and post hoc LSD analysis test displayed no significant difference in each group. This could be due to the material used as a conditioner prior to the GIC application. The addition of fluoride ions will inhibit the demineralization process of the enamel produced by the application of conditioner. So it does not rule out that it will interfere with the attachment of the GIC material used as a fissure sealant in this research.Conclusion: There was no significant difference in shear bond strength of fissure sealant based on glass ionomer between sodium fluoride and acidulated phosphate fluoride topical application.

Shear bond strength fissure sealant based on glass ionomer after topical fluor application: a comparison between sodium fluoride and acidulated phosphate fluoride Akwan, Yulie Emilda; Paramita, Annisa Listya; Rahmitasari, Fitria
Odonto : Dental Journal Vol 9, No 2 (2022): December 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.2.222-230

Background: Application of fissure sealants to young permanent teeth is one of the preventive measures for caries in the field of dentistry. Fissure sealants based on glass ionomer cement (GIC) have the advantage of releasing fluoride. Sodium fluoride and acidulated phosphate fluoride (APF) are remineralization agents because they can increase remineralization by altering hydroxyapatite to fluorapatite and this in turn makes fluorine able to increase pH. Fluoride can prevent caries by increasing remineralization of the enamel that has just been demineralized by acids produced by plaque bacteria. The aim of this study is to compare the shear bond strength fissure sealant based on glass ionomer between sodium fluoride and APF topical fluor applicationMethod: This research applied true experimental design with post test only control group design. The 21 maxillary permanent premolars were distributed into 3 groups, namely the control group (K1), the treatment group added 5% sodium fluoride (K2), and the treatment group added 1.23% APF (K3). Result: One Way ANOVA and post hoc LSD analysis test displayed no significant difference in each group. This could be due to the material used as a conditioner prior to the GIC application. The addition of fluoride ions will inhibit the demineralization process of the enamel produced by the application of conditioner. So it does not rule out that it will interfere with the attachment of the GIC material used as a fissure sealant in this research.Conclusion: There was no significant difference in shear bond strength of fissure sealant based on glass ionomer between sodium fluoride and acidulated phosphate fluoride topical application.

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