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Characteristics of chitosan from Penaeus monodon on chitosan-gelatin suspension viscosity Prananingrum, Widyasri; Setiawan, Grace Caroline; Putri, Vindya Meinda; Wahyuning, Baiq Nadia Risna; Rahmitasari, Fitria; Sularsih, Sularsih; Ramadhania, Nurina Rizka; Fatmawati, Sri
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 2 (2025): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i2.p135-143

Abstract

Background: Chitosan synthesized from Penaeus monodon shells was developed into a chitosan-gelatin suspension as an injectable bone substitute for socket preservation. Purpose: To investigate the characteristics of chitosan from P. monodon shells and their influence on the viscosity of a chitosan-gelatin suspension. Methods: P. monodon shells from Tarakan Waters were prepared using three methods: Group 1) deproteinization-depigmentation-deacetylation, Group 2) demineralization-depigmentation-deacetylation, and Group 3) deproteinization-demineralization-depigmentation-deacetylation. The chitosan was characterized by morphology, moisture and ash content, molecular weight (MW), deacetylation degree (DD), and viscosity. This chitosan was made into a chitosan-gelatin suspension with a ratio of 45:55 (w/w%) (95 ml:110 ml). The differences in viscosity of the chitosan-gelatin suspension were determined using Kruskal–Wallis and Mann–Whitney tests. The effects of the chitosan’s MW and DD on the viscosity of the chitosan-gelatin suspension were analyzed using Spearman’s correlation. Results: Group 2 had the highest moisture content (10.63%), MW (159.68 kDa), viscosity of the chitosan powder (5.53 dPa.s), and viscosity of the chitosan-gelatin suspension (40.20 cps). Group 1 had the highest ash content (27.83%) and DD (93.72%). Group 3 showed the lowest ash content (1.06%), MW (37.12 kDa), and DD (86.22%), but it had good viscosity of the chitosan-gelatin suspension (37.25 cps). A significant difference in viscosity was found between the chitosan-gelatin suspension groups. Spearman’s correlation coefficients between the viscosity of the chitosan-gelatin suspension and MW and between the viscosity of the chitosan-gelatin suspension and DD were 0.389 and −0.195, respectively. Conclusion: The viscosity of a chitosan-gelatin suspension is influenced by the MW and DD of the chitosan powder. Chitosan from P. monodon can potentially be an injectable bone substitute in socket preservation.
UPAYA PENINGKATAN WAWASAN DALAM MENJAGA KESEHATAN GIGI DAN MULUT PADA GURU DAN ORANG TUA SISWA TK CAHAYA TAZKIA SURABAYA Rahmitasari, Fitria; Lestari, I.G.A.M Oka; Nilawati, Nina; Setianingtyas, Dwi; Yoyada, Novendy; Tantra, Ivan; Indarti, Caecilia; Prabowo, Puguh Bayu; Emilda, Yulie; Hartono, Mardiyanto Riski; Irmawati, Ari Rosita; Ayu A, Shintya Rizki
Jurnal Pengabdian Masyarakat Pesisir VOLUME 3 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v3i1.96

Abstract

Angka proporsi perilaku menyikat gigi secara benar sebanyak dua kali sehari yaitu setelah sarapan pagi dan sebelum tidur malam hanya sebesar 2,8% pada masyarakat Indonesia. Sejumlah 93% anak-anak usia sekolah mengalami masalah gigi dan mulut, dimana angka ini merupakan angka prevalensi yang sangat tinggi. Upaya Kementerian Kesehatan Republik Indonesia dalam menurunkan angka prevalensi karies gigi yaitu dengan melaksanakan program “Indonesia Bebas Karies 2030”. PT. Unilever Tbk. melakukan kerjasama dengan Asosiasi Fakultas Kedokteran Gigi Indonesia (AFDOKGI), Asosiasi Rumah Sakit Gigi dan Mulut Pendidikan Indonesia (ARSGMPI), Persatuan Dokter Gigi Indonesia (PDGI), dan Fakultas Kedokteran Gigi Universitas Hang Tuah akan melakukan program “School Health Program” (SHP) dalam rangkaian kegiatan Bulan Kesehatan Gigi Nasional (BKGN) 2022. Program Training of Trainer (ToT) pada guru dan orang tua untuk kegiatan sikat gigi pagi dan malam 21 hari mengajarkan anak-anak dan orang dewasa tentang manfaat kebersihan mulut yang baik dan menunjukkan kepada mereka cara menyikat gigi dua kali sehari dengan pasta gigi berfluoride untuk mewujudkan Indonesia bebas karies di tahun 2030. Kegiatan ini mengunakan sistem digital / online melalui aplikasi zoom. Peserta kegiatan ini adalah 5  orang tua dan 5 guru di TK Cahaya Tazkia. Dalam memberikan edukasi, para dokter gigi menggunakan media power point dan video edukasi. Bentuk materi edukasi yang diberikan meliputi video animasi sikat gigi, kalender digital sikat gigi 21 hari, poster/ flyer digital, flipchart. Sebelum memulai edukasi para peserta dilakukan pre test terlebih dahulu tentang pengetahuan seputar gigi dan kebersihannya sesuai materi yang akan disampaikan, setelah diakhir sesi zoom tersebut juga dilakukan post test dengan materi yang sama untuk mengetahui tingkat pengetahuan dan pemahaman peserta terhadap materi tersebut. Kegiatan penyuluhan kesehatan gigi dan mulut melalui ToT pada guru dan orang tua siswa/siswi di TK Cahaya Tazkia Surabaya memberikan hasil peningkatan tingkat pengetahuan guru dan orang tua dalam menjaga kesehatan gigi dan mulut. Hasil analisis statistik menggunakan paired sample t-test untuk membandingkan nilai rata-rata pre test dan post test siswa/siswi menunjukkan nilai signifikansi p=0.001 (p<0.05) yang berarti bahwa terdapat perbedaan yang signifikan pada tingkat pengetahuan guru dan orang tua siswa/siswi di TK Cahaya Tazkia dalam menjaga kesehatan gigi dan mulut sebelum dan sesudah diberi materi Dental Health Education (DHE) melalui “Training of Trainer”.  
TRAINING OF TRAINER (TOT) DALAM UPAYA PEMELIHARAAN KESEHATAN GIGI UNTUK ORANG TUA DAN SISWA DI SD RADEN PAKU SURABAYA Rahmitasari, Fitria; Rosita Irmawati, Ari; Revianti, Syamsulina; Nilawati, Nina; Widyastuti; Kurniawan, Hansen; Bayu Prabowo, Puguh; Pangabdian, Fani; Nur Ashrin, Meinar; Widaningsih; Handayani, Budi; Khoironi, Emy; Lukisari, Cane; Samadi, Karlina; Harnowo, Setyo
Jurnal Pengabdian Masyarakat Pesisir VOLUME 3 NOMOR 2
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v3i2.120

Abstract

Angka kejadian permasalahan kesehatan gigi dan mulut di Indonesia mencapai 57,6%, dimana tergolong sangat tinggi, dengan 93% di antaranya dialami oleh anak-anak usia sekolah. Pengetahuan, sikap, dan perilaku individu memiliki peran yang penting dalam menjaga kesehatan gigi mulut. Lingkungan sekitar anak, terutama keluarga dan guru, memiliki peran yang krusial dalam membentuk sikap dan perilaku mereka. School Health Program (SHP) yang melibatkan pelatihan Training of Trainer (ToT) mengenai kesehatan gigi mulut bagi guru dan orang tua mencakup kegiatan menyikat gigi dua kali sehari selama 21 hari. Program ini bertujuan untuk memberikan pendidikan kepada anak dan orang tua beserta guru tentang perlunya menjaga oral hygiene yang baik serta mengajarkan teknik menggosok gigi dengan pasta gigi berfluoride. Inisiatif ini juga mendukung pencapaian target "Indonesia Bebas Karies 2030" dan dilaksanakan secara daring melalui aplikasi Zoom. Pemahaman peserta terkait kesehatan gigi dievaluasi melalui kuesioner sebelum dan sesudah diberikan pelatihan. Analisis statistik menggunakan uji t-sampel berpasangan untuk mengetahui dan membandingkan nilai rerata sebelum dan sesudah diberikan pelatihan menunjukkan nilai p<0,05, yang mengindikasikan adanya peningkatan pengetahuan yang signifikan mengenai pemeliharaan kesehatan gigi mulut pada guru serta orang tua siswa di SD Raden Paku melalui program ToT. Kegiatan ini terbukti efektif dalam meningkatkan pengetahuan dan perilaku mengenai kesehatan gigi dan mulut setelah materi diberikan. 
The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Rahmitasari, Fitria; Cevanti, Twi Agnita; Prabowo, Puguh Bayu; Sularsih, Sularsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Abstract

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups
Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Zefanya, Kartika; Rahmitasari, Fitria; Widaningsih, Widaningsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Abstract

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.
Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Zefanya, Kartika; Rahmitasari, Fitria; Widaningsih, Widaningsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Abstract

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.
The effect of incorporating hydroxyapatite into Type II glass ionomer cement on flexural strength and the examination of fractured surfaces using scanning electron microscopy Rahmitasari, Fitria; Cevanti, Twi Agnita; Prabowo, Puguh Bayu; Sularsih, Sularsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.152-161

Abstract

Background: Glass ionomer cement (GIC) is widely recognized as the prevailing direct esthetic restorative material. In order for a restoration material to be considered effective, possess favorable physical and mechanical properties. Incorporating with hydroxyapatite (HA) can provide these properties. This research is to investigate the impact of integrating HA into GIC on the flexural strength and scanning electron microscopy (SEM) of the fractured surfaces. Methods: The study consisted of 28 samples of GIC, which are fabricated in the shape of rectangular prisms (25x2x2mm). The samples were divided into four groups n=7. Group 1 is a control group (GIC), Group 2 GIC+2%HA, group 3 GIC+2%HA, and GIC+4%HA. The flexural strength of the sample was evaluated by a Universal testing machine, followed by an examination of the fracture surface using SEM. Result: The Brown-Forsythe was used as data analysis to examine the flexural strength values across all groups, resulting in a statistically significant p-value of less than 0.05. The addition of HA does not result in a significant increase in the flexural strength value of the glass ionomer cement (GIC). The integration of microstructure based on scanning electron microscopy (SEM) pictures demonstrates improved visual quality following the incorporation of HA. Conclusion: The addition of HA to GIC did not result in a significant change in flexural strength compared to the control group. However, GIC group with 2% HA exhibited the highest average flexural strength value among all the groups
Tegillarca granosa shell combination with Vitis vinifera and fluoride in decreasing enamel microporosity Setiawan, Grace Caroline; Tinisia, Adelia; Rahim, Muhammad Galang Adhinata Abdul; Rahmitasari, Fitria; Prananingrum, Widyasri
Dental Journal (Majalah Kedokteran Gigi) Vol. 57 No. 2 (2024): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v57.i2.p131-138

Abstract

Background: White spot lesion is a demineralization process indicated by the increased of enamel microporosity. A tegillarca granosa shell contains 98.7% calcium and Vitis vinifera contains phytochemical compounds with fluoride, which has a potential to stimulate remineralization. Purpose: To analyze the Tegillarca granosa shell combination with Vitis vinifera and fluoride in decreasing enamel microporosity. Methods: The cream was prepared by combining 10% and 20% Tegillarca granosa shell with 10 grams of Vitis vinifera extract and 100 mg of fluoride. The cream was tested beforehand for viscocity and pH. Furthermore, 16 premolars were etched and divided into four groups. Group 1 was smeared with placebo (negative control) and Group 2 was smeared with casein phosphopeptide-amorphous calcium phosphate (positive control). The other groups were smeared with cream 10% (Group 3) and 20% (Group 4) Tegillarca granosa shell combination with Vitis vinifera and fluoride. Teeth were treated three times a day for 30 minutes and soaked in artificial saliva. After 14 days, the enamel microporosity was carried out using a scanning electron microscope. The data was analyzed with one-way analysis of variance (ANOVA) test followed by post-hoc least significant difference (LSD). Results: The enamel microporosity showed significant difference between Group 1 and the other groups. There was no significant difference between Groups 2, 3, and 4 (p<0.05). Although there was no significant difference between Group 3 and 4, the lowest one was in Group 4 (p>0.05). Conclusion: The cream, prepared by combining Tegillarca granosa shell with Vitis vinifera and fluoride, is effective in decreasing the enamel microporosity.