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All Journal ODONTO Dental Journal Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Jurnal Material Kedokteran Gigi Odonto dental journal Jurnal Pengabdian Masyarakat Bangsa JPMP Indonesian Journal of Community Empowerment
Fitria Rahmitasari
Department Of Dental Materials, Faculty Of Dentistry, Universitas Hang Tuah, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Kartika Zefanya; Fitria Rahmitasari; Widaningsih Widaningsih; Widyasri Prananingrum
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Abstract

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.
Particle size analysis of yellowfin tuna (Thunnus albacares) skin collagen powder using papain-soluble collagen method with varying NaCl concentrations: an experimental laboratory Fatryana, Destya Arizha; Rahmitasari, Fitria; Nanik K., Chaterina Diyah; Rizal, Moh. Basroni; Winias, Saka
Padjadjaran Journal of Dentistry Vol 36, No 2 (2024): July 2024
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no2.54588

Abstract

ABSTRACTIntroduction: The skin of yellowfin tuna (Thunnus albacares) contains high protein, which could potentially be a halal collagen product. Collagen extraction using the papain-soluble collagen method has the advantage of producing a higher collagen yield compared to the acid method. Particle size, one of the physical properties of collagen, plays a crucial role in its efficacy in dentistry. This study aims to analyze the particle size of collagen powder synthesized   from Thunnus albacares skin using the papain-soluble collagen method, with varying concentrations of NaCl. Methods: Type of research is an experimental laboratory. Thunnus albacares skin was synthesized by chopping, cleaning, and soaking in a 0.1 M NaOH solution. The extraction process used the enzyme papain and 0.5 M of acetic acid. Samples were divided into four groups, each with different concentrations of NaCL: 0, no NaCL;, 0.9 M NaCl, 1.3 M NaCl, and 1.7 M NaCl. After centrifugation, the samples were freeze-dried. The particle size of collagen powder was measured using a Particle Size Analyzer test tool. The data collected was then analyzed using the Mann-Whitney test. Results: Particle size distributions are as follows: K group (3.36 nm), P1 (1.842 nm), P2 (3.36 nm), and P3 (10.12 nm). There is a significant difference in groups K-P1 and P3, P1-P2 and P3, and P2-P3 (p<0.05). However, there is no significant difference in groups K-P2 (p>0.05). Conclusion: Particle size of this research produced nano-sized collagen powder, with the lowest particle size observed in the 0.9 M NaCl group, measuring at 1.842 nm. The particle size increased in the group without NaCl and in the 1.3 M NaCl group at 3.36 nm, and reached the highest value in the 1.7 M NaCl group at 10.12 nm. 
Pendidikan Dan Pelatihan Kader Sebagai Salah Satu Pilar Penting Perubahan Perilaku Di Bidang Kesehatan Gigi Dan Mulut Pranoto, Amelia Elizabeth; Aprilia, Aprilia; Rahmitasari, Fitria; Soesilo, Diana; Puspita, Sinta; Riski, Mardiyanti; Ariestania, Vivin; Lestari, IGAM Oka; Raharjo, Pambudi; Wijaya, Yongki Hadinata; Rizal, Mohammad Basroni; Sarianoferni, Sarianoferni; Hendro, Onge Victoria; Wira, Rizko; Sularsih, Sularsih; Cahyo, Benny Dwi; Paramita, Putri
Jurnal Pengabdian Masyarakat Bangsa Vol. 2 No. 11 (2025): Januari
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v2i11.1930

Abstract

Lima dari sepuluh anak-anak Indonesia usia sekolah dasar memiliki masalah gigi berlubang. Hal ini disebabkan oleh kebersihan rongga mulut yang buruk dan kurangnya pengetahuan anak-anak terkait pentingnya menjaga kesehatan gigi dan mulut. Anak-anak juga seringkali tidak menyikat gigi di waktu yang benar, ditambah lagi cara menyikat gigi yang keliru menjadi salah satu faktor munculnya gigi berlubang. Dokter gigi dari Fakultas Kedokteran Gigi, Universitas Hang Tuah Surabaya melakukan kegiatan pengabdian masyarakat berupa School Health Program – Training of Trainer (SHP-ToT). Kegiatan ini bertujuan untuk menciptakan kader kesehatan gigi dan mulut yang berasal dari lingkungan internal sekolah. Diharapkan kader-kader tersebut dapat menjadi pilar penggerak utama pada pendidikan anak-anak usia sekolah dasar akan pentingnya menjaga kebersihan gigi dan mulut secara berkelanjutan. Kader akan diberi pelatihan dan diuji sejauh mana pemahamannya terkait kesehatan gigi dan mulut. Setelah pelatihan selesai, kader akan terus dipandu untuk mengajarkan anak-anak sekolah dasar tentang pentingnya menjaga kesehatan rongga mulut sekaligus menjadi supervisor anak-anak agar mau menyikat gigi pagi dan malam selama 21 hari berturut-turut.
INDEKS DMF-T dan def-t MURID USIA 7-10 TAHUN MI TARBIYATUT THOLABAH LAMONGAN Kurniawan, Hansen; Fitriani, Yufita; Sari, Rima Parwati; Handayani, Budi; Rahmitasari, Fitria; Prabowo, Puguh Bayu; Aprilia, Aprilia; Ariestania, Vivin; Priambodo, Nur Tsurayya; Saptaswari, Dianty
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 1 (2025): MAJU : Indonesian Journal of Community Empowerment, Januari 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/fwwcfg92

Abstract

Background Based on the 2018 Basic Health Research (Riskesdas), it was reported that the prevalence of dental and oral problems in Indonesia is still very high, namely 57.6 percent. Of this figure, only 10.20% have received treatment. Dental and oral health in Indonesia, especially in coastal areas, is still a complex problem. (Komarudin, Apriasari and Azizah, 2021) Lack of public attention and equal distribution of health facilities, especially in the field of Dentistry, are still factors that influence public understanding of dental and oral health. Dental and oral health status can be assessed using the DMF-T and def-t indices. Objective To assess the dental and oral health status in children aged 7-10 years MI Tarbiyatut Tholabah, Paciran Lamongan Method Dental and oral examination of children aged 7-10 years MI by assessing the DMF-T and def-t indices. Conclusion The results of the dental and oral examination of students at MI Tarbiyatut Tholabah, Paciran Lamongan with a def-t value of 2.71 which means the dental and oral health status with moderate results, and the DMF-T index with a result of 0.56 which means very low.
TRAINING OF TRAINER (TOT) DALAM UPAYA PEMELIHARAAN KESEHATAN GIGI UNTUK ORANG TUA DAN SISWA DI SD RADEN PAKU SURABAYA Fitria Rahmitasari; Ari Rosita Irmawati; Syamsulina Revianti; Nina Nilawati; Widyastuti; Hansen Kurniawan; Puguh Bayu Prabowo; Fani Pangabdian; Meinar Nur Ashrin; Widaningsih; Budi Handayani; Emy Khoironi; Cane Lukisari; Karlina Samadi; Setyo Harnowo
Jurnal Pengabdian Masyarakat Pesisir VOLUME 3 NOMOR 2
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v3i2.120

Abstract

Angka kejadian permasalahan kesehatan gigi dan mulut di Indonesia mencapai 57,6%, dimana tergolong sangat tinggi, dengan 93% di antaranya dialami oleh anak-anak usia sekolah. Pengetahuan, sikap, dan perilaku individu memiliki peran yang penting dalam menjaga kesehatan gigi mulut. Lingkungan sekitar anak, terutama keluarga dan guru, memiliki peran yang krusial dalam membentuk sikap dan perilaku mereka. School Health Program (SHP) yang melibatkan pelatihan Training of Trainer (ToT) mengenai kesehatan gigi mulut bagi guru dan orang tua mencakup kegiatan menyikat gigi dua kali sehari selama 21 hari. Program ini bertujuan untuk memberikan pendidikan kepada anak dan orang tua beserta guru tentang perlunya menjaga oral hygiene yang baik serta mengajarkan teknik menggosok gigi dengan pasta gigi berfluoride. Inisiatif ini juga mendukung pencapaian target "Indonesia Bebas Karies 2030" dan dilaksanakan secara daring melalui aplikasi Zoom. Pemahaman peserta terkait kesehatan gigi dievaluasi melalui kuesioner sebelum dan sesudah diberikan pelatihan. Analisis statistik menggunakan uji t-sampel berpasangan untuk mengetahui dan membandingkan nilai rerata sebelum dan sesudah diberikan pelatihan menunjukkan nilai p<0,05, yang mengindikasikan adanya peningkatan pengetahuan yang signifikan mengenai pemeliharaan kesehatan gigi mulut pada guru serta orang tua siswa di SD Raden Paku melalui program ToT. Kegiatan ini terbukti efektif dalam meningkatkan pengetahuan dan perilaku mengenai kesehatan gigi dan mulut setelah materi diberikan. 
Characteristics of chitosan from Penaeus monodon on chitosan-gelatin suspension viscosity Prananingrum, Widyasri; Setiawan, Grace Caroline; Putri, Vindya Meinda; Wahyuning, Baiq Nadia Risna; Rahmitasari, Fitria; Sularsih, Sularsih; Ramadhania, Nurina Rizka; Fatmawati, Sri
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 2 (2025): June
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i2.p135-143

Abstract

Background: Chitosan synthesized from Penaeus monodon shells was developed into a chitosan-gelatin suspension as an injectable bone substitute for socket preservation. Purpose: To investigate the characteristics of chitosan from P. monodon shells and their influence on the viscosity of a chitosan-gelatin suspension. Methods: P. monodon shells from Tarakan Waters were prepared using three methods: Group 1) deproteinization-depigmentation-deacetylation, Group 2) demineralization-depigmentation-deacetylation, and Group 3) deproteinization-demineralization-depigmentation-deacetylation. The chitosan was characterized by morphology, moisture and ash content, molecular weight (MW), deacetylation degree (DD), and viscosity. This chitosan was made into a chitosan-gelatin suspension with a ratio of 45:55 (w/w%) (95 ml:110 ml). The differences in viscosity of the chitosan-gelatin suspension were determined using Kruskal–Wallis and Mann–Whitney tests. The effects of the chitosan’s MW and DD on the viscosity of the chitosan-gelatin suspension were analyzed using Spearman’s correlation. Results: Group 2 had the highest moisture content (10.63%), MW (159.68 kDa), viscosity of the chitosan powder (5.53 dPa.s), and viscosity of the chitosan-gelatin suspension (40.20 cps). Group 1 had the highest ash content (27.83%) and DD (93.72%). Group 3 showed the lowest ash content (1.06%), MW (37.12 kDa), and DD (86.22%), but it had good viscosity of the chitosan-gelatin suspension (37.25 cps). A significant difference in viscosity was found between the chitosan-gelatin suspension groups. Spearman’s correlation coefficients between the viscosity of the chitosan-gelatin suspension and MW and between the viscosity of the chitosan-gelatin suspension and DD were 0.389 and −0.195, respectively. Conclusion: The viscosity of a chitosan-gelatin suspension is influenced by the MW and DD of the chitosan powder. Chitosan from P. monodon can potentially be an injectable bone substitute in socket preservation.
Co-Authors Adelia Tinisia Andriani, Dwi Annisa Listya Paramita Aprilia Aprilia, Aprilia Ari Rosita Irmawati Ariestania, Vivin Arifzan Razak Budi Handayani Caecilia Indarti Cahyo, Benny Dwi Cane Lukisari Diana Soesilo, Diana Dwi Setianingtyas Elly Munadziroh Emy Khoironi Ester Arijani Rachmat Fani Pangabdian Fatryana, Destya Arizha Grace Caroline Setiawan Handayani, Budi Hansen Kurniawan Hendro, Onge Victoria I.G.A.M Oka Lestari Ivan Tantra Karlina Samadi Kartika Zefanya Kurniawan, Hansen Lestari, IGAM Oka Mardiyanto Riski Hartono Meinar Nur Ashrin Muhammad Galang Adhinata Abdul Rahim Nanik K., Chaterina Diyah Nina Nilawati Novendy Yoyada Pranoto, Amelia Elizabeth Priambodo, Nur Tsurayya Puguh Bayu Prabowo Puguh Bayu Prabowo Puguh Bayu Prabowo, Puguh Bayu Puspita, Sinta Putri Paramita Putri, Vindya Meinda Raharjo, Pambudi Ramadhania, Nurina Rizka Retno Pudji Rahayu Rima Parwati Sari, Rima Parwati Riski, Mardiyanti Rizal, Moh. Basroni Rizal, Mohammad Basroni Saka Winias Saptaswari, Dianty Sarianoferni Setiawan, Grace Caroline Setyo Harnowo Shintya Rizki Ayu A SRI FATMAWATI Sularsih Sularsih Sularsih Sularsih Sularsih, S Syamsulina Revianti Twi Agnita Cevanti Wahyuning, Baiq Nadia Risna Widaningsih Widaningsih Widaningsih Widyasri Prananingrum Widyastuti Wira, Rizko Wisnu Setyari J Yongki Hadinata Wijaya Yufita Fitriani Yulie Emilda