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Widaningsih Widaningsih, Widaningsih
Politeknik Negeri Malang Jl. Soekarno Hatta No. 9 Malang
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Journal : ODONTO Dental Journal

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Viability of BHK-21 Fibroblast Cells Resulting from Synthesis of Collagen Powder (Thunnus albacares) with Variation of NaCl Concentration Zefanya, Kartika; Rahmitasari, Fitria; Widaningsih, Widaningsih; Prananingrum, Widyasri
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.94-104

Abstract

Background: Collagen plays a crucial role in post-extraction wound healing. Thunnus albacares skin, a collagen source synthesized through the papain soluble collagen (PaSC) method with varying NaCl concentrations, is assessed for non-toxicity using the MTT assay on BHK-21 fibroblast cell cultures. This research is to determine cell viability resulting from synthesizing collagen powder from the skin of yellowfin tuna (Thunnus albacares) with varying NaCl concentrations using the papain soluble collagen (PaSC) method. Method: Collagen powder was synthesized by cleaning Thunnus albacares skin measuring 1x1 cm, soaking it in 0.1 M NaOH, hydrolyzing it with CH3COOH, extracting it with papain enzyme, and then dividing the samples into four groups: without NaCl, NaCl 0.9 M, NaCl 1.3 M, and NaCl 1.7 M. Samples were centrifuged (6000 rpm) and followed by freeze-drying. Cell viability was obtained by conducting a cytotoxicity test using the MTT Assay method on BHK-21 fibroblast cells. Result: The percentage of cell viability in groups K, P1, P2, P3, and P4 were 100%, 10.708%, 113.750%, 107.833%, and 105.958%, respectively. The Kruskall-Wallis test yielded a significance value 0.000, indicating a significant difference (p<0,005). The Mann-Whitney test confirmed significant differences between groups. Conclusion: Collagen powder from Thunnus albacares skin with NaCl concentrations of 0.9 M, 1.3 M, and 1.7 M showed no toxic effects, while the group without NaCl showed toxic effects. Collagen powder with a NaCl concentration of 1.7 M yielded ideal results and showed no toxic effects.
Co-Authors Abdul Chalim Agnita Cevanti, Twi Ahmad Bahauddin Almu’faro Ahmad Bahauddin Almu’faro Akmala Hadita Amalia, Hanny Anisa Anindya Pinasti, Restika Arya Brahmanta, Arya Bayu Hikmat Purwana, Bayu Hikmat Damaiyanti, Dian Widya Dhaifullah, Muhammad Najmi Diana Soesilo, Diana Dika Rizky Yunianto, Dika Rizky Dwi Budi Santoso Dwi Maharani, Aulia Fadloli Fadloli Fani Pangabdian, Fani Fitria Rahmitasari Handayani, Budi Hanum, Faizah Hariyanti Hariyanti Harnowo, Setyo Hendrawan, Muhammad Afif Iman, Dimas Indra Dharma Wijaya, Indra Dharma Istighna, Luthfiah Nur Kamila, Nisrina Cahya Khoironi, Emy Kusumawardhani, Khalyssa Putri Lelyna, Nora Listya Paramita, Annisa Lokman, Asmidar Mahpudin Mahpudin Mawardy, Vivy Mohamad Sinal Muhammad Shulhan Khairy Mulyandika, Gilang Arival Nilawati, Nina Noengki Prameswari Novi Agustina Nugroho, Muhammad Bintang Nurdiansah, Erdin Nurlia, Elly Nuryanah, Nuryanah Paramita, Anisa Listya Paramita, Ayulistya Pinasti, Restika Anindya Puguh Bayu Prabowo, Puguh Bayu Pupu Pupung Pundenswari, Pupung Puspita, Sinta Rahman, Kurnia Hayati Rahmanawati, Ainin Rizal Maulana, Rizal Rizal, Mohammad Basroni Santoso, Nanda Trio Septian Enggar Sukmana Setiani, Fadila Bela Sihabuddin, Sihabuddin Sinambela, Helen F Sinurat, Henri Prianto Sucahyo, Bambang Suhariningsih Susi Adiawaty Sutarto, Ayulistya Paramita Syamsulina Revianti Ulumudin, Aceng Wawan Wawan Wedarti, Yoifah Rizka Widyasri Prananingrum Yuliyani, Zilha Yuningsih, Miranti Arum Zefanya, Kartika