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All Journal Jurnal Bios Logos JSME MIPA UNIMA Jurnal Produksi Tanaman Indonesian Journal of Biotechnology Jurnal Sains dan Teknologi Pangan BIOMA : Jurnal Biologi Makassar Jurnal Biologi Tropis JURNAL PENDIDIKAN TAMBUSAI Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus SOSCIED Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Makara Journal of Science Indonesian Biodiversity Journal Rumphius Pattimura Biological Journal Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika Jurnal Pengabdian Masyarakat dan Riset Pendidikan Indonesian Biodiversity Journal Polygon: Jurnal Ilmu Komputer dan Ilmu Pengetahuan Alam Algoritma: Jurnal Matematika, Ilmu Pengetahuan Alam, Kebumian dan Angkasa J-CEKI Konstanta : Jurnal Matematika dan Ilmu Pengelatuan Alam Advances in Tropical Biodiversity and Environmental Sciences
Helen Joan Lawalata
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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IDENTIFICATION OF ESCHERICHIA COLI AND SALMONELLA SP. ON FRIED SNACKS SOLD ON THE UNIMA CAMPUS ENVIRONMENT Runtunuwu, Yudistira Deyvan; Lawalata, Helen J; Tengker, Anita
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10756

Abstract

This study aims to explain how bacterial contamination and the presence of Escherichia coli and Salmonella sp. on fried bananas sold in the UNIMA campus environment. This exploration used clear qualitative subjective observation with the TPC strategy for all total bacterial contamination and involved specific media for Escherichia coli and Salmonella sp. From the results of the review, it was found that 1 out of 3 experimental examples of complete bacterial contamination exceeded the threshold set by the Food and Drug Supervisory Agency of the Republic of Indonesia (BPOM RI) in 2016, with the most extreme obstacle being the number of microorganisms in food being 10-4 colonies/ g. Of the 3 samples tried, all samples were identified with Escherichia coli and Salmonella sp. Based on the research conducted, it can be concluded that, of all the observed fried samples, all detected bacterial colonies
Isolation and Identification of Lactic Acid Bacteria from Red Dragon Fruit (Hylocereus polyrhicus) as Exopolysaccharide Producers Tatebale, Rivaldo; Naharia, Orbanus; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.7730

Abstract

Cherry tomatoes are a type of Red Dragon Fruit that has various benefits, including lowering cholesterol levels, preventing colon cancer, and strengthening the working power of muscles. Isolation of LAB isolated from Dragon Fruit as a production material for EPS. This study aims to isolate and identify LAB as a producer of EPS from red dragon fruit (Hylocereus polyrhiruz), which can produce exopolysaccharides. This research uses a descriptive research method. Data from experimental research in the laboratory obtained 10 LAB isolates, namely isolates NG1, NG2, NG3, NG4, NG5, NG6, NG7, NG8, NG9 and NG10. Based on the identification results, isolate NG1 has similarities with the genus Lactococcus (spherical, gram-positive, nonmotile, non-spore). While isolates NG2, NG3, NG4, NG5, NG6, NG7, NG8, NG9 and NG10 have similarities with the genus Lactobacillus (rod form, nonmotile, gram-positive). Based on the morphological characteristics, which are gram-positive, catalase-negative and non-spore. The ten isolates of lactic acid bacteria are capable of producing EPS. these are the results of EPS acquisition, namely: NG1(152.1 mg/L), NG2(127.9 mg/L), NG3(134.6 mg/L), NG4(130.9 mg/L), NG5(137 mg/L), NG6(139.2 mg/L), NG7(204.9 mg/L), NG8(156.2 mg/L), NG9(136.4 mg/L), and NG10 (157, 3 mg/L). The highest amount of EPS was isolated NG7 at 204.9 mg/L. Meanwhile, the lowest EPS was isolated NG4 at 130.9 mg/L.
APPLICATION OF Eco-Enzyme CONVERSION RESULTS INTO DISHWASHER SOAP Tedang, Bybyani; Roring, Verawati Ida Yani; Moko, Emma Mauren; Posumah, Danny Christian; Lawalata, Helen J
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10752

Abstract

The application of the conversion results from Eco-Enzyme into dishwashing soap, the fermentation process lasts for one months, where the resulting liquid is dark brown in color and has a very strong sweet and sour fermented aroma. There is a lack of public knowledge about the very profitable benefits of Eco-Enzyme, such as as a multi-purpose cleaner as a multi-functional liquid which can be converted, one of the ways, into dishwashing soap. This research aims to determine the application of the results of converting Eco-Enzyme into dishwashing soap. This research used experimental methods and a research period of 3 months. This Eco-Enzyme is made with a ratio of organic ingredients: sugar: water 3:1:10. The Eco-Enzyme harvest is converted into dishwashing soap by adding lerak and Methyl Ester Sulfonate (MES). Eco-Enzyme conversion soap was then applied to several treatment groups: plastic plates, cans and glass. The results of the application show that the dishes washed with Eco-Enzyme using lerak have not completely removed dirt, oil and odors in each treatment, whereas Eco-Enzyme using Methyl Ester Sulfonate (MES) when compared with Sunlight brand liquid soap sold on the market shows that there are similarities both in terms of cleanliness, tapestry and smell. The research conclusion is that the application of the Eco-Enzyme conversion results has significant potential to be developed into an active ingredient for environmentally friendly dishwashing soap.
ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

Abstract

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category
TESTING FOR BACTERIAL CONTAMINATION ON CHICKEN (Gallus gallus domesticus) IN THE TRADITIONAL MARKET OF SOUTH LANGOWAN DISTRICT Sambeka, Delvino; Mege, Revolson; Setyawati, Iriani; Lawalata, Helen J; Rompas, Christny Ferdina Evie
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10754

Abstract

Chicken meat (Gallus gallus domesticus) plays an important role in society with its role as one of the sources of fulfillment of animal protein in Indonesia. With the high consumption of chicken meat by the public, the hygiene and safety of chicken meat must be ensured so as not to incur losses to the people. This study aimed to determine the bacterial contamination based on the testing of Total Plate Counts, Coliform, Escherichia coli, and Salmonella sp. Meet the requirement of the Indonesian National Standard on chicken meat in the traditional market of Langowan Selatan District. The method used in this research is a descriptive method to analyze and describe the results of the study, the testing using several indicators to directly conclude bacterial contamination. The results of the total plate count bacterial contamination test of the colonies, TPC in sample 1 = 29,1x kol/, sample 2 = 29,4x kol/, sample 3 = 27,7x kol/. MPN of Coliform and Escherichia coli results obtained in samples S1, S2, and S3 are 11x kol/gr. The results of microbact Salmonella sp. In samples S1 are positive, and S2 and S3 are negative. Based on observations, it is suspected that contamination occurs because sellers use equipment that is less sterile and there is no cover or barrier so the chicken meat is in direct contact with the air and customer. It is concluded that based on bacterial testing indicators, chicken meat in the traditional market of Langowan Selatan District has exceeded the limit of SNI 7388 2009 and is contaminated.
IDENTIFICATION OF ESCHERICHIA COLI AND SALMONELLA SP. ON FRIED SNACKS SOLD ON THE UNIMA CAMPUS ENVIRONMENT Runtunuwu, Yudistira Deyvan; Lawalata, Helen J; Tengker, Anita
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10756

Abstract

This study aims to explain how bacterial contamination and the presence of Escherichia coli and Salmonella sp. on fried bananas sold in the UNIMA campus environment. This exploration used clear qualitative subjective observation with the TPC strategy for all total bacterial contamination and involved specific media for Escherichia coli and Salmonella sp. From the results of the review, it was found that 1 out of 3 experimental examples of complete bacterial contamination exceeded the threshold set by the Food and Drug Supervisory Agency of the Republic of Indonesia (BPOM RI) in 2016, with the most extreme obstacle being the number of microorganisms in food being 10-4 colonies/ g. Of the 3 samples tried, all samples were identified with Escherichia coli and Salmonella sp. Based on the research conducted, it can be concluded that, of all the observed fried samples, all detected bacterial colonies
DEVELOPMENT OF AN ANTI-MOSQUITO SPRAY GEL FORMULATION FOR A COMBINATION OF MARIGOLD FLOWERS (TAGETES ERECTA L.) AND CHRYSANTHEUM FLOWERS (CHRYSANTHEMUM INDICIUM L.) Mulder, Keysia Stephanie Tesalonica; Mokosuli, Yermia Semuel; Lawalata, Helen Joan
Jurnal Review Pendidikan dan Pengajaran Vol. 7 No. 1 (2024): Volume 7 No 1 Tahun 2024
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v7i1.24062

Abstract

Mosquitoes are vectors of infectious diseases that must be controlled. One of the efforts to control mosquito vectors is by using anti-mosquito products, including anti-mosquito gel spray. Most anti-mosquito products are composed of chemicals that are harmful to health and the environment, so alternative anti-mosquito products made from natural ingredients are needed. The aim of this research is to determine an effective anti-mosquito gel spray formula from a combination of marigold flowers and chrysanthemum flowers. This research uses experimental methods to find the most effective gel spray formulation from different percentages of marigold and chrysanthemum flowers. The variations used are 0%, 2.5%, 5% and 10%. Tests were carried out using 30 adult female mosquitoes. The results obtained through the extraction of marigold flowers showed a yield of 8.922% and a yield of chrysanthemum flowers of 4.560%. The organoleptic test carried out on 10 panelists showed that on average the panelists thought they liked (in the range 4-5) their preference for color and aroma. , texture and reaction after 10 minutes of using the gel spray. The pH test showed that the four gel spray formulas had a pH of 6 or neutral. The results of the homogeneity test carried out with a glass object showed that the four gel spray formulas were homogeneous. The protective power of the 2.5% gel spray formula is 18.01%; gel spray formula 5% is 66.29% and gel spray formula 10% is 88.61%. From these results, it can be seen that a formula with a concentration of marigold and chrysanthemum flower extracts of 10% can be the most effective anti-mosquito gel spray. It is hoped that the results of this research will be useful for society in terms of using mosquito repellent that is environmentally friendly and safe for health.
Pengaruh Penerapan Model Pembelajaran Guided Discovery Learning Menggunakan Pendekatan Scientific Terhadap Hasil Belajar Siswa Marchio Yosua Gratia Mamesah; Meyke Paat; Helen Joan Lawalata
Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa Vol. 2 No. 3 (2024): Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa
Publisher : Asosiasi Riset Ilmu Matematika dan Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/algoritma.v2i3.57

Abstract

This research aims to determine the effect of the guided discovery learning model on student learning outcomes in class 12 using a scientific approach; in this research, the researcher used 2 learning methods, one of which is the conventional method; the researcher used 2 classes as samples, where each class was 15 students in each class, was coded as class A for the control class and class B for the experimental class. The research instrument used was a question in the form of a posttest. 
Relationship Between Length and Weight and Habitat Conditions of Several Fish Species in Tondano Lake, Minahasa, North Sulawesi Rosenta Sipayung; Helen Joan Lawalata; Iriani Setyawati; Livana Dethris Rawung; Rievo Handry Djarang
Advances in Tropical Biodiversity and Environmental Sciences Vol. 7 No. 3 (2023): October 2023: Pages 87-124
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2023.v07.i03.p01

Abstract

The snake-head gudgeon or payangka fish (Ophieleotris aporos), tilapia fish or mujair (Oreochromis mossambicus), and marble goby or betutu fish (Oxyeleotris marmorata) are economically important fish in Lake Tondano. However, numerous community activities can disrupt aquatic biodiversity, declining Lake Tondano environmental conditions, and other factors. This study aimed to determine the relationship between length and weight and the habitat conditions of Ophieleotris aporos, Oreochromis mossambicus, and Oxyeleotris marmorata. The method used in this study was purposive sampling with research stations located at Remboken (station 1), Kakas (station 2), and Tondano Estuary (station 3). Sampling was carried out 5 times in every 10 days. The weight and length of the fishes caught in Lake Tondano were 2-60 g and 1-8 cm (Ophieleotris aporos); 9-180 g and 6-25.5 cm (Oreochromis mossambicus); and 16-30 g and 2.6-26.5 cm (Oxyeletotris marmorata), all of them have an allometric growth pattern. Oreochromis mossambicus is the most commonly caught fish in all research stations. The research station with the best level of water fertility is Remboken Station. The habitat characteristic of Lake Tondano was 25-290C of water temperature; brightness of 1.34-2.66 m; depth of 4.23-11.8 m; pH of 6.7-7.8; and dissolved oxygen of 2.27-6.31 mg/L, which still supports the well-being of the fish.
Mangrove Biodiversity In Tatengesan Village, Pusomaen District, Southeast Minahasa Nofia Poluan; Helen Joan Lawalata; Wiesye Maya Selfia Nangoy; Meity Neltje Tanor; Rievo Handry Djarang
Advances in Tropical Biodiversity and Environmental Sciences Vol. 8 No. 1 (2024): February 2024: Pages 1-57
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2024.v08.i01.p10

Abstract

Mangrove forest is a type of forest that grows in coastal areas and river estuaries and has three functions, namely ecological, social, and economic functions. The purpose of this study was to determine the level of diversity of mangrove species in Tatengesan Village, Pusomaen District, Southeast Minahasa Regency. Observations were made on a transect with a size of 50 x 25 m consisting of 4 plots. Mangrove sampling was carried out using a plot with a size of 10 x 7 m, using a purposive sampling method. Ecological index analysis includes species diversity, uniformity, and dominance. There are 7 mangrove species namely Bruguiera gymnnorhiza, Rhizophora mangle, Kandelia obovata, Avicennia marina, Rhizophora mucronata, Languncularia racemose, and Ceriops tagal. The species diversity index at the biological level is low.
Co-Authors Akay, Fanesa Andini Maurent Dwiyanti Anita Constanci Christine Tengker Anita Costanci Christine Tengker Anita Costanci Christine Tengker Aser Yalindua Bricita Regina Warangkiran Christny Ferdina Evie Rompas Danny Christian Posumah Daud, Putri Debby Jacqualine Jochebed Rayer Decky D. W. Kamagi Decky David Wewurid Kamagi Delvy Regina Leunufna Dino Rahardiyan Elsa Aru Emma M Moko Endang Sutriswati Rahayu Enjel Larega Esibrena Br Kemit Esrah Anna Adeleid Agimat Etin Ruban Febiola Gabriela Tular Feby Febrianty Toding Rante Fernando Andre Watung Futwembun, Fransiska Gabrielle Warouw Genade Kojongian Helena, Maria Helma Fitriani Herry Maurits Sumampouw Hotni Doani Purba Iriani Setyawati IRIANI SETYAWATI Iriani Setyawati Jantje Ngangi Jefry Jack Mamangkey Jelita Pricilya Tompolumiu Kansile, Elma Fridayanti Kembuan, Patricia Pericilia Langkah Sembiring LANGKAH SEMBIRING Letsoin, Imanuel Junisa Adolfina Lidya Yangse Lumowa Livana Dethris Rawung Luis Sandi Najoan Maiko Polandos Manampiring, Nonny Maradesa, Sartika Marchio Yosua Gratia Mamesah Mariana Rengkuan Marsanda Tandiapa Marthy L S Taulu Marthy Lingkan Stela Tauluh Marthy Lingkan Stella Taulu Marthy Stella Taulu Megawati Meylin Weken Meity Nelltje Tanor Meity Neltje Tanor Melany Piri Mercy Maggy Franky Rampengan Metilistina Sasinggala Meyke Paat Michellia Pamela Sanggor Miracle Abbighael Varencia Welan Moko, Emma Mokoginta, Ayatola R. Mokosuli Yermia Samuel Mokosuli, Yeremia Samuel Mulder, Keysia Stephanie Tesalonica Muli, Efel J. R. Nangoy, Wiesye M. S> Natalina Zalukhu Nofia Poluan Nonny Manampiring Nova L.I.M Ogi Nuriyati Hadia Orbanus Naharia Orbanus Naharia Pelealu, Irna W. Posumah, Danny Posumah, Danny Christian Rahardiyan, Dino Rahayu, Endang Sutriswati Rampengan, Mercy Maggy Franky Regina R. Butarbutar Revanda Stevanie Hasani Revolson Mege Rey, Feyne Jelita Rievo Djarang Rievo Handry Djarang Rompas, Christny Ferdina Evie Roring, Verawati I. Y Roring, Verawati I. Y. Rosenta Sipayung Runtunuwu, Yudistira Deyvan Salaila, Tania Jennet Sambeka, Delvino Sambuaga, Natalia V. Samuel, Mokosuli Y. Simbolon, Kristina Br Sisilia Domitila Sangur Tanorl, Meity N. Tatebale, Rivaldo Tedang, Bybyani Theresia Viviani Putri Ndese Tuelah, Syela Nathasya Utari Satiman Verawati I Y Roring Verawati I.Y. Roring Verawati I.Y. Roring Verawati Ida Yani Roring Watugigir, Gerfindo Y. G Wiesye Maya Selfia Nangoy Wiesye Nangoy Yeremia S. Mokosuli Yermia Samuel Mokosuli YOSUA LINU Yulianus Rompah Matana Zusje Wiesje Merry Warouw