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Mentari, Ika Nurfajri
Politeknik Medica Farma Husada Mataram
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The Effectiveness of Betel Leaf (Piper betle) Extract on the Growth of Staphylococcus epidermidis Pauzan, Pauzan; Mentari, Ika Nurfajri; Kurniawan, Ari
Jurnal Biologi Tropis Vol. 25 No. 4a (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4a.10604

Abstract

Staphylococcus epidermidis, a gram-positive bacterium typically found on the skin, can cause infections, particularly in individuals with implanted medical devices. Due to its ability to resist standard antibiotics and create biofilms, finding new antibacterial solutions is crucial. Betel leaves (Piper betle) are recognized for containing active components like flavonoids, saponins, polyphenols, and essential oils, suggesting they could act as natural antibacterial agents. This research sought to assess how well green betel leaf extract inhibits S. epidermidis growth in a controlled laboratory setting. The research involved a laboratory-based experiment utilizing a completely randomized design. The disc diffusion method on Nutrient Agar media was employed to test three extract concentrations (40, 60, and 80 mg/disk), using erythromycin as a positive control and DMSO as a negative control. Each treatment was repeated four times. The diameter of the inhibition zones was measured following a 24-hour incubation period at 37°C. The Shapiro–Wilk normality test, one-way ANOVA, and Tukey HSD test were used to analyze the data collected. The findings revealed that the concentrations of 60 and 80 mg/disk exhibited substantial antibacterial effects, producing an average inhibition zone of 14.25 mm, which was significantly different from the negative control (p < 0.05). In conclusion, green betel leaf extract is effective at suppressing the growth of S. epidermidis and shows promise as a source for developing a natural antibacterial treatment.
Co-Authors Adriyan Suhada Ageng Abdi Putra Aini aini aini aini Aini, Aini aini, aini aini Ajeng Dian Pertiwi, Ajeng Dian Amirah Andi Maya Kesrianti Anjelin, Desak Putu Ardiansyah, Lalu Dwi Sartia ARI KURNIAWAN Aulia, Nurhikmatul Ayu, Mirah Adhi I Gusti BaiqIsti Hijriani Bustanul Atfal Deby Anggryani Desyani Ariza Desyani Ariza Dini Eka Fitriani Edy Kurniawan, Edy Eka Fitria Fardani, Roushandy Asri Hadi, Suryatno Hery Widijanto Idham Halid Irawansyah Irawansyah Ismatullah, Nurul Khatimah Kurniawati, Ari Laelatur Zahrah Maulana, Alfi Mindi Richia Putri Mohd Nazil Bin Salleh Muhadi, Muhadi Ni Nyoman Ariwidiani Nurhikmatul Aulia Nurul Hadiatun Pauzan Pauzan Pauzan, Pauzan Permana, Yan Reiza Prasati, Supriana Putri, Pipit Sagita Rachman, Muhammad Aditya Ratulangi, Wulan Ratia Reni Chairunnisah, Reni Rian Wahyul Ikhtiar Roushandy Asri Fardani Santriono, Hadi Saptiana, Baiq Agustin Sri Idawati Suhartiningsih Suhartiningsih Sukmana, Dhika Juliana sunardi sunardi Sunardi Sunardi Syahrul Syamsuriansyah, Syamsuriansyah Ulya, Tuhfatul ustiawaty, jumari Ustyawati, Jumari Wijaya, Alwan Zahrah, Laelatur