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All Journal International Journal of Public Health Science (IJPHS) Jurnal Kesehatan Lingkungan indonesia Althea Medical Journal Jurnal Penelitian Kesehatan Suara Forikes Health Notions 2-TRIK: TUNAS-TUNAS RISET KESEHATAN Journal of Community Empowerment for Health Media Gizi Kesmas International Journal Of Science, Technology & Management (IJSTM) Diversity: Disease Preventive of Research Integrity Edu Dharma Journal: Jurnal penelitian dan pengabdian masyarakat AloHA International Journal of Health Advancement (AIJHA) Moluccas Health Journal An Idea Health Journal Global Health Science (GHS) Aloha International Journal of Multidisciplinary Advancement (AIJMU) JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Jurnal Sains dan Kesehatan Medical Laboratory Journal Jurnal Pengabdian Kepada Masyarakat Radisi Obat: Jurnal Riset Ilmu Farmasi dan Kesehatan Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) Jurnal Kesehatan Maluku Husada
Sillehu, Sahrir
STIKes Maluku Husada
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Transportation

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Journal : Althea Medical Journal

 1 
Var Gene Encoding Duffy-Binding Like (DBL) 1α- Plasmodium Falciparum Erythrocyte Membrane Protein 1 (PfEMP1) as Diagnostic Marker and Clinical Predictor Candidates for Falciparum Malaria Dewi, Rosita; Sulistyaningsih, Erma; Aprilia, Annisa Nadya; Kusuma, Irawan Fajar; Sillehu, Sahrir
Althea Medical Journal Vol 11, No 4 (2024)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v11n4.2396

Abstract

Background: The pathogenesis of severe malaria involves the antigenic protein Plasmodium falciparum erythrocyte membrane protein-1 (PfEMP1), encoded by the var gene. One of the important domains in PfEMP1 is Duffy Binding Like 1α (DBL1α). To diagnose malaria, microscopic examination has low sensitivity and specificity, therefore, the development of molecular-based methods is needed. This study aimed to determine the potential of DBL1α-PfEMP1 as a diagnostic marker and clinical predictor for falciparum malaria.Methods: An exploratory descriptive study was conducted in 2019 on malaria patients at the Tiakur public health center, Southwest Maluku, Indonesia. Blood samples of patients infected with Plasmodium falciparum malaria were collected on filter paper for DNA isolation. Amplification by polymerase chain reaction (PCR) method used primers αAF [5'-GCA CG(A/C) AGT TTT GC-3'] and αBR [5'-GCC CAT TC(G/C) TCG AAC CA-3'] with cycles of denaturation 95oC 1-minute, annealing 42oC 1-minute, elongation 60oC 1-minute. PCR products were electrophoresed using 1% agarose gel. Amplicons were sequenced directly and analyzed using nucleotide BLAST-NCBI. Results: All patients showed mild malaria symptoms. PCR amplification yielded bands of 370 bp in all samples and 600 bp in 8 out of 10 samples, and 1 sample had a different pattern. Sequencing results confirmed that the amplicon was DBL1α, a var gene that had similarities to sequences from other regions.Conclusion: Positive amplification and sequencing results confirm the sensitivity of DBL1α-PfEMP1 as a diagnostic marker. The sequence variability of PCR product implies the presence of DBL1α variations, indicating a correlation with clinical outcomes and making it a clinical predictor.
Co-Authors Abd. Rijali Lapodi Abdul Rohim Tualeka Acob, Joel Rey U Acob, Joel Rey U. Akollo, Isak Roberth Alan Pattilouw Aprilia, Annisa Nadya Apriliawati, Anita Apriliawati, Anita Arief Budiono Atik Badiah Bambang Hadi Sugito Basir, Haun Abd Catur Prasastia Dewi Lukita Destanul Aulia, Destanul Dewi Kartika Eka Safitri Sillehu Endah Fitriasari Epi Dusra Erlin Kiriwenno Erma Sulistyaningsih Evi Irianti Fatima Tatroman Febby Sonya Matulessy Frisca P.A Wattimena Hariyanto, Rohadi Heny Arwati Heru Santoso Wahito Nugroho Hidayati, Syariefah I Putu Suiraoka Ibrahim, Ilyas ilyas - ibrahim Ilyas Ibrahim Indah Susanti Indriati Paskarini Ira Fanty Wakan Irawan Fajar Kusuma Istifara, Anindia Iswandi Fataruba Joel Rey U. Acob Joel Rey U. Acob Joel Rey Ugsang Acob Koekoeh Hardjito Lameky, Vernando Yanry Lapodi, Abd Rijali Lating, Zulfikar Lestari & Catur Prasatia, Indah Lukman La Basy M Dahlan Sely Majida, Narwan Makayaino, Hafis Manik, Hubban Fathani Mariene Wiwin Dolang Maryam Lihi Maryam Lihi Maryam Lihi, Maryam Mohammad Dahlan Sely Muhammad Azril Hardiman Mahulauw Muhammad Rakib Latuconsina Muhammad Taufan Umasugi Ni Made Parwati Noer Saudah Pius Selasa Revita Savitri Sebe Rohadi Hariyanto Rosita Dewi Rumaolat, Wiwi Salakory, Hans Sammy Marthin Sanglar Polnok Sartika Maya Sary Sumanjaya Sely, Mohammad Dahlan Setiawan Sheena Ramadhia Asmara Dhani Sinay, Herlien Siti Rahmawaty Tombalissa Siti Rochmaeda Soulissa, Fathmy F Sudjajadi Keman Sunarto, Sunarto Sunik Cahyawati Sunik Cahyawati Sunik Cahyawati Suparji Suparji Suparji Suparji Syariefah Hidayati Syariefah Hidayati Waliulu Syulce Luselya Tubalawony Taufan Umasugi Tina Amnah Ningsih Tunny, Hasna Tunny, Ira Sandi Tunny, Rahma Utami, Tri Niswati Victor E. D Palapessy Wiwi Rumaolat Wiwin Martiningsih Yoes Prijatna Dachlan