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DEVELOPMENT OF A NITROCELLULOSE MEMBRANE-BASED TEST STRIP FOR DETECTING MERCURY (II) Dyah Wulandari, Devyana; Muhammad Fadillah, Farchan; Ulan Sari, Della Citra; Mufida, Nisaul; Wilujeng, Putri Shinta
Walisongo Journal of Chemistry Vol. 8 No. 2 (2025): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v8i2.26705

Abstract

Mercury (Hg), a hazardous heavy metal, has become a significant environmental and public health concern. Practical tools for detecting mercury in water and cosmetic samples remain limited. This study aimed to develop a nitrocellulose membrane–based test strip capable of detecting mercury through its reaction with diphenylcarbazide. The development process involved the formation of a purple-colored complex resulting from the interaction between mercury ions and diphenylcarbazide, which served as the visual indicator of mercury presence. The selectivity of the test strip was evaluated against non-mercury ions, and its accuracy (%) was compared to the atomic absorption spectroscopy (AAS) method. The results revealed that the nitrocellulose membrane–based test strip was selective toward mercury ions, exhibited a clear color response with increasing mercury concentrations, and demonstrated a relatively high accuracy of 83.33%. These findings suggest that the developed test strip has strong potential as a semi-quantitative and portable tool for mercury detection.
Analisis Efek Mitragynine Terhadap Metabolisme Glukosa melalui Uji Toleransi Glukosa (OGTT) dan Uji Toleransi Insulin (ITT) pada Model Tikus Diabetes Awwalia, Evi Sylvia; Faradis, RA. Hani; Wulandari, Devyana Dyah; Salim, Hotimah Masdan
Prominentia Medical Journal Vol 7 No 1 (2026): Prominentia Medical Journal
Publisher : Universitas Ciputra Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37715/pmj.v7i1.6054

Abstract

Mitragynine is the major alkaloid found in Mitragyna speciosa (kratom) and has been reported to possess various biological activities, including potential effects on glucose metabolism. However, scientific evidence regarding its role in glucose tolerance and insulin sensitivity remains limited. This study aimed to evaluate the effects of methanolic extract of Mitragyna speciosa containing mitragynine on glucose metabolism through the Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT) in experimental animals. Experimental animals were divided into groups consisting of a control and treatment groups receiving methanolic extract of M. speciosa at doses of 15 mg/kg BW and 30 mg/kg BW. Parameters observed included blood glucose levels at different time points following glucose administration (OGTT) and the response to exogenous insulin administration (ITT). Administration of mitragynine extract significantly reduced blood glucose levels compared with the control group in the OGTT, particularly at the 30 mg/kg BW dose. Furthermore, the ITT demonstrated improved insulin sensitivity, with a more rapid decline in blood glucose levels in the treatment groups. These effects showed a dose-dependent pattern, with the higher dose producing more pronounced improvements. Methanolic extract of Mitragyna speciosa containing mitragynine has the potential to enhance glucose metabolism by improving glucose tolerance and increasing insulin sensitivity. These findings suggest that mitragynine could be explored as a potential adjuvant agent in managing glucose metabolism disorders; however, further investigations are needed to elucidate its molecular mechanisms and long-term safety.
THE EFFECT OF BEETROOT (Beta vulgaris L.) JUICE ON CHOLINESTERASE ACTIVITY IN FARMERS EXPOSED TO ORGANOPHOSPHATE PESTICIDES Wulandari, Devyana Dyah; Santoso, Andreas Putro Ragil; Wulansari, Devyani Diah
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 1 No 2 (2019): Laboratory Analysis Applied to Medical Properties
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v1i2.1215

Abstract

A decrease in cholinesterase (chE) enzyme activity is an indicator of pesticide poisoning, especially organophosphate pesticides. The chE activity reduction will result in an increase in the amount of acetylcholine and will bound to muscarinic and nicotinic receptors in the central and peripheral nervous system which can lead to the onset of Alzheimer's disease. However, antioxidant products can slow the progression of Alzheimer's disease by protecting neurons from oxidative stress. Red Beetroot (Beta vulgaris L.) contains betalains and phenolic compounds which act as an antioxidants that are capable of preventing such illness. This study aims to prove the concept that antioxidant compounds in red beetroot juice can increase the chE activity in subjects exposed to organophosphate pesticides. This research is an observational study with an experimental approach. This research used 25 farmers who were exposed to organophosphate pesticides as respondents. They were given 500 ml of beetroot juice twice a day for 2 consecutive months. Cholinesterase levels were measured before and after consuming red beet juice. Measurement of cholinesterase levels was carried out using a kinetic photometric test. This method was developed based on recommendations from the German Society of Clinical Chemistry (DGKC). The results showed that the average of chE (U/L) Level Before Treatment was 8.102 and 8.380 after treatment with a p value 0,62. It can be concluded that there was an increase in the level of cholinesterase but it was not statistically significant. This may be caused by a different response to activity of cholinesterase after consuming beetroot juice in each subjects, age difference, smoking habits, and personal protective equipment.
CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT Andini, Ary; Prayekti, Endah; Dyah Wulandari, Devyana; Nidianti, Ersalina
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i1.1467

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.
PENINGKATAN PENGETAHUAN DETEKSI MOLEKULER PADA TENAGA KESEHATAN MENGGUNAKAN METODE SEMINAR ONLINE Endah Prayekti; Devyana Dyah Wulandari; Ary Andini; Gilang Nugraha; Septina Isro’ Hermawati; Qonita Iklila Maghfuroh; Imraatul Mukarramah; Lili Susan Rahmawati; Luqiana Fauziah
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 5 No. 6 (2024): Vol. 5 No. 6 Tahun 2024
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v5i6.37610

Abstract

Perkembangan teknologi biologi molekuler menjadi sebuah terobosan baru untuk mendeteksi sumber infeksi sehingga dapat membantu dalam proses diagnosis. Metode biologi molekuler mempunyai beberapa keunggulan diantaranya lebih sensitif, lebih spesifik, dan lebih cepat. Kelemahan yang harus dihadapai dari perubahan metoode deteksi ini adalah peningkatan kemampuan tenaga Kesehatan yang bergerak dibidang tersebut untuk mempermudah penguasaan teknologi. Tujuan kegiatan ini adalah untuk meningkatkan pengetahuan tenaga Kesehatan dalam diagnostic molekuler. Metode yang digunakan dalam mencapai tujuan adalah menggunakan seminar secara daring menggunakan platform zoom. Sasaran kegiatan adalah tenaga Kesehatan yang berkecimpung dilaboratorium pemeriksaan Kesehatan. Pengukuran keberhasilan kegiatan dilihat dengan hasil peserta dalam menjawab post test dan dinyatakan lulus pada laman Plataran Sehat. Peserta yang mengikuti kegiatan adalah sebanyak 185 orang, dan 157 orang dinyatakan lulus, 5 orang dinyatakan tidak lulus. Berdasarkan hasil kegiatan ini dapat disimpulkan kegiatan yang dilakukan secara online berhasil meningkatkan pengetahuan peserta
Co-Authors , Wirda Nur Fa'ida Adela Puspitasari Aditiya Putri, Ngiluhtara Dyah Aguslina Kirtishanti Aguslina Kirtishanti Aini, Fadlilah Nur Aishella, Maulida Akhmad, Nur Faradila Alfian Daffa Alkatiri, Wardah Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ardyarini Dyah Savitri Ardyarini Dyah Savitri Arvian Nur Amalina Audina, Miea Awwalia, Evi Sylvia Ayu Wuryaningrum Baskara, Muhamad Damar Bintarti, Tri Wahyuni Choirotussanijjah Choirotussannijah Choirotussannijah Damar Baskara, Muhammad Dyah Dedi, Muhamad Derrizkie Divanda Herzhaputra Devyani Diah Wulansari Devyani Diah Wulansari Devyani Dyah Wulansari Dewi Masitah Dwi Handayani Emma Savitri Endah Budi Permana Putri Ersalina Nidianti Eva Tri Wulandari Fadlilah Nur Aini Faradis, RA. Hani Fitriana Sari, Devi Gilang Nugraha Gilang Nugraha Halimah Prisilia Herliani Rahmania Sari Hotimah Masdan Salim Illahi, Wahyu Imastufah Imraatul Mukarramah Imratul Mukarramah Indah Lestari Janetra Yudhis Nata P Kifli, Nurolaini Krisdayanti, Affina Lili Susan Rahmawati Lili Susan Rahmawati Luqiana Fauziah Lysa Veterini Ma'at, Suprapto Mella Rahmawati Mukminin Mellyisa Mochamad Nurul Lail Mufida, Nisaul Muhammad Afwan Romdloni Muhammad Fadillah, Farchan Muhammad Taufiq Hidayat Mukhtar Adi Nugroho Nadzarillah, Najmatul Jannah Najwa Asnani, Abidah Dyah Nanik Handayani Novera Herdiani, Novera Nur Lailatul Qodriyah Nur Zalsabila Isnaini Nuzula, Fina Firdausi Pratiwi, Viera Nuriza Prayekti, Endah Qonita Iklila Maghfuroh Raden Joko Kuncoroningrat Susilo Rahmawati Fitri Awalia Risthanti, Reine Risa Rizki Nurmalya Kardina Rohmah, Wardah Rosda Rodhiyana S. P. Edijanto Penulis Safinah Izzatunnisa Salim, Hotimah Masdan Sari, Herliani Rahmania Septina Isro’ Hermawati Suprapto Maat Suryandaru, Suryandaru Syafira, Nanda Syafiuddin, Achmad Syinta Nur Isnaini Ulan Sari, Della Citra Uswatun Hasanah Wardah Rohmah Wardany, Nadya Ika Widya Novita Sari Widya Novitasari Wilujeng, Putri Shinta Wulansari, Devyani Wulansari, Devyani Diah Wulansari, Devyani Diah Wuryaningsih, Lucia Endang Yusron Suryonegoro Mushawwir Zain, R.A Wafiqah Aulia