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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,439 Documents
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Pengaruh Lama Penyimpanan Pengencer Tris Kuning Telur Terhadap Motilitas Spermatozoa Sapi Simental di BIB Banyumulek Provinsi Nusa Tenggara Barat Janah, Maratun; Arifpansyah, Akbar; Atma, Candra Dwi; Dharmawibawa, Iwan Doddy
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.14109

Abstract

Tris yolk is a diluent that can be used to maintain and protect spermatozoa during storage so that they can be used in the process of artificial insemination. The length of storage greatly affects the motility and viability of spermatozoa in semen. The purpose of this study was to determine the effect of long storage of egg yolk tris diluent on the motility and viability of simental bovine spermatozoa. This research was carried out at the Banyumulek Artificial Insemination Center Laboratory, West Nusa Tenggara Province, from July to Desember 2024. The study of the effect of long storage of egg yolk tris diluent on the motility and viability of simental bovine spermatozoa used a randomized experimental method complete with four treatments and six repeats. Each treatment, namely P1, P2, P3, and P4, was carried out by mixing 0.075 ml of semen and 0.3 ml of egg yolk tris diluent stored for 24, 48, 72, and 96 hours. The data obtained were processed by variety analysis method (ANOVA) and Duncan's further test. The results showed that P1 treatment with a storage period of 24 hours resulted in the highest average motility and viability values of 63.33 ± 6.055 % for motility and 63.50 ± 6.892 % for spermatozoa viability. P4 treatment with a storage period of 96 hours still showed a good mean value with motility values of 40.83 ± 7.360 % and spermatozoa viability of 53.50 ± 12.112 %. Thus, egg yolk tris is suitable for use as a semen thinner for artificial insemination because it is able to maintain the motility and viability of spermatozoa even for a long time of 96 hours.
Physicochemical and Organoleptic Properties of Mixed Mulberry (Morus alba) Jam at Different Temperatures and Aloe Vera (Aloe vera) Concentrations Ramadhani, Amalia; Hartati, Fadjar Kurnia; Rahmiati, Retnani
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.14148

Abstract

This study aims to determine the effects of temperature and Aloe vera concentration on the physicochemical and organoleptic properties of mixed mulberry (Morus alba) jam. Jam is a semi-solid fruit and sugar-based product that requires a thickening agent to achieve optimal texture. Aloe vera was selected as a natural thickening alternative due to its polysaccharide content, such as acemannan and glucomannan, which can form gels, replacing expensive and import-dependent commercial pectin. The research employed a quantitative experimental method with a Completely Randomized Design (CRD), involving four treatment combinations of Aloe vera concentration (75 g and 100 g) and cooking temperature (70°C and 85°C). The parameters tested included physicochemical properties such as viscosity, fiber content, and vitamin C levels, along with organoleptic evaluations involving 25 panelists to assess viscosity and taste. Data were analyzed using analysis of variance (ANOVA) and an effectiveness test to identify the best treatment. The results showed that the combination of 100 g Aloe vera concentration and a cooking temperature of 85°C produced the best-quality jam, characterized by optimal viscosity, high fiber content, preserved vitamin C levels, and high panelist preference for viscosity and taste. This conclusion highlights the potential of Aloe vera as a cost-effective and value-added natural thickening agent for the food industry.
Formulasi Sabun Mandi Cair Berbasis Virgin Coconut Oil (VCO): Optimalisasi Pemanfaatan Produk Lokal Untuk Perawatan Kulit dan Pencegahan Dehidrasi Maulina, Novia; Muslikh, Faisal Akhmal; Nastiti, Ginanjar Putri; Fadilla, Rahma Nur; Winayah, Mesri Ananta; Kamelin, Badira; Firdaus, Natasya Octavia Putri; Fricananta, Salma Salsabila; Dewi, Tanaya Jati Dharma
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14160

Abstract

Skin dehydration is a common issue affecting 40–50% of the adult population, resulting in dry, rough, and dull skin, as well as itching, irritation, and premature aging. The "back to nature" trend has increased interest in natural skincare products that are more environmentally friendly and gentler on the skin. Virgin coconut oil (VCO) is a popular natural ingredient known for its ability to maintain skin moisture and provide protection. This study aims to develop a liquid soap formulation using VCO to address skin dehydration. The research methods included formulation and evaluation of liquid soap through organoleptic testing, foam stability, specific gravity, pH, as well as hedonic and irritation tests. The results showed that: (1) Formula III met all physical evaluation parameters, including organoleptic characteristics, foam stability and height, specific gravity, and pH, in accordance with SNI standards; (2) Formula III was the most preferred by panelists in the hedonic test and caused no skin irritation based on irritation test results. Therefore, Formula III can be concluded as the optimal formulation for VCO-based liquid bath soap.
Hubungan Anemia, Tingkat Stres, Aktivitas Fisik, dan Konsumsi Makanan Cepat Saji dengan Kejadian Dismenore Primer Pada Siswi SMA IT Putri Abu Hurairah Mataram Nirmala, Suci; Novitasari, Novitasari; Rahadianti, Dian; Sudiarta, I Gede
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.14163

Abstract

Dysmenorrhea is severe pain in the lower abdomen during menstruation. Dysmenorrhea is one of the health problems that has a high incidence rate worldwide. The purpose of this study was to determine the relationship between anemia, stress levels, physical activity and fast food consumption with the incidence of primary dysmenorrhea in SMA IT Putri Abu Hurairah Mataram. This research is an observational analytic quantitative research with a cross-sectional study design. The population of this study were all 374 students of SMA IT Putri Abu Hurairah Mataram. The research instruments used were questionnaires in the form of WaLLID score questionnaire, DASS questionnaire, International physical activity questionnaire short form (IFAQ-SF), and Food Frequency Questionnaire (FFQ). Data were analyzed using Spearman, univariate, and bivariate tests. The results showed that (1) there was no significant relationship between anemia and primary dysmenorrhea; (2) there was a significant relationship between stress level and primary dysmenorrhea; (3) there was a significant relationship between physical activity and primary dysmenorrhea; (4) there was a significant relationship between fast food consumption and primary dysmenorrhea.
Pengelolaan Ekowisata Mangrove Berkelanjutan Sebagai Sarana Edukasi dan Pembelajaran IPA di Bale Mangrove Jerowaru Kabupaten Lombok Timur Idrus, Agil Al; Gunada, I Wayan; Idawati, Sri; Nirmala, Siska Ayu; Yahdi, Yahdi; Munib, Munib; Dewi, Earlyna Sinthia
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14164

Abstract

This study aims to explore how ecotourism management involving environmental education can increase community awareness and involvement in mangrove conservation. This research design is descriptive qualitative research. The research sample was determined randomly for visitors to Bale Mangrove Ecotourism. The research instrument used questionnaire sheets and interview guidelines with managers, visitors, and the surrounding community as well as observation sheets of ecotourism practices in the area. The research data consisted of responses from structured questionnaires, interviews and qualitative analysis methods, including thematic coding and content analysis, were used to categorize and interpret the responses. The results showed that mangrove ecotourism in Bale Mangrove not only provides economic benefits to the local community, but also acts as an effective educational medium to increase knowledge about the importance of mangrove conservation. With an approach that involves community empowerment and environmental education, it is expected that the management of this ecotourism can provide a sustainable model that benefits both economically and ecologically.
Pengaruh Konsentrasi Sodium Alginat Terhadap Boba Kulit Buah Naga (Hylocereus Polyhizus) Menggunakan Teknik Spherifikasi Dasar Ningrum, Cicik Agustia; Yuniati, Yuyun; Sucahyo, Bambang Sigit
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.14179

Abstract

Usually, dragon fruit is only used for the flesh while the skin is discarded and considered waste. Dragon fruit peel contains vitamin C, vitamin E, vitamin A, alkaloids, terpenoids, flavonoids, thiamine niacin, pyridoxine, cobalamin, phenolics, carotene and phytoalbumin. 30-35% of dragon fruit is the skin of the fruit but it is often just thrown away as trash. Dragon fruit skin contains quite a lot of the natural dye anthocyanin. This research aims to determine the effect of pH, water content, vitamin C, and organoleptics on the of dragon fruit skin boba using the basic spherificatiom technique. This research used laboratory experimental methods using a completely randomized design (CRD) with varying concentrations of sodium alginate (1gr,1,1gr,1,2gr,1,3gr, dan 1,4gr) calcium lactate (5 grams) for each treatment. This was repeated 3 times, the analyzes tested in this study were pH, viscosity and organoleptic tests. The parametric data obtained were analyzed using analysis of variance (ANOVA) using statistical product and service solution (SPSS) version 23 data and for non-parametric data using Kruskal Wallis.
Lama Fermentasi dan Konsentrasi Bunga Chamomile (Matricarica recutita) Terhadap Mutu Fisikokimia dan Organoleptik Teh Kombucha Fahilah, Khoiriyah; Hartati, Fadjar Kurnia; Yuniati, Yuyun
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.14182

Abstract

Kombucha is a health drink that has good benefits for the body, contains polyphenols and is antioxidant so it is said to be a functional drink. Chamomile positively contains flavonoid compounds, polyphenols, and tannins as antioxidants to ward off free radicals. The purpose of this study was to determine the effect of fermentation duration and concentration of chamomile flowers (Matricaria recutita) to produce kombucha drink with the best physicochemical and organoleptic quality. This study used a one-factorial Completely Randomized Design consisting of four treatments of a combination of fermentation duration (5 days and 7 days) and chamomile concentration (0.5% and 1%), each treatment was carried out three times. Parameters analyzed included color (L*a*b*), pH, antioxidant activity and organoleptic (taste, color, and aroma). The results showed that the length of fermentation and chamomile concentration had a significant effect on color (L*a*b*), pH, antioxidant activity and organoleptic parameters (taste and color). On organoleptic parameters (aroma) did not have a significant effect. The best treatment with antioxidant activity value of 67.09% in treatment F2C2 (7 days fermentation time and 1% chamomile concentration). The highest organoleptic quality was in the F1C1 treatment (5 days fermentation and 0,5% chamomile concentration).
Ekplorasi Senyawa Ekstrak Etanol Rumput Belulang (Eleusine indica (L.) Gaertn) Menggunakan LC-MS/MS-QTOF Serta Uji Aktivitas Anti-Diare: In-Vivo Pratama, Rizki Rahmadi; Septianto, Andre; Al-Hasyimi, Said Muhammad; Herliana, Herliana; Hasniah, Hasniah; Muslikh, Faisal Akhmal
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.14192

Abstract

Diarrhea is one of the diseases caused by symptoms of intestinal tract infections due to various microorganisms. Goosegrass (Eleusine indica (L.) Gaertn) is a wild weed that has long been used as a traditional medicine, attributed to its phytochemical compounds such as tannins, alkaloids, flavonoids. This study aims to evaluate the antidiarrheal effects of Eleusine indica extract by comparing six treatment groups: a healthy group, a negative control group (Na-CMC), a positive control group (Loperamide 2 mg), and three variations of the extract dosage. Observations were made over 360 minutes at 30-minute intervals, using a fecal consistency scale from 1 (solid) to 5 (liquid). LC-MS/QToF analysis identified 25 secondary metabolites in the extract, including flavonoids (Schaftoside, Orientin, Vitexin, Tricin), phenolic compounds, and terpenoids. In vivo testing showed that the third dose of Eleusine indica grass extract had the most optimal antidiarrheal effect among the three doses, as evidenced by its ability to reduce diarrhea frequency, decrease fecal weight, shorten diarrhea duration, and improve fecal consistency, approaching the effectiveness of Loperamide 2 mg as a positive control. These findings suggest the potential of Eleusine indica extract as a promising natural antidiarrheal agent.
Evaluasi Kemampuan Literasi Biodiversitas Mahasiswa Fajri, Siti Rabiatul; Citrawathi, Desak Made; Adnyana, Putu Budi; Arnyana, Ida Bagus Putu
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.14208

Abstract

Indonesia is one of the countries with the highest biodiversity in the world. However, this high diversity requires individual awareness and responsibility to maintain and utilize it sustainably. Therefore, biodiversity literacy is an important element, especially for students as prospective future generations who will play a role in natural resource management in the future. This study aims to evaluate the level of biodiversity literacy of students of the Biology study program at the University of Education Mandalika, by assessing six dimensions of biodiversity literacy: Conservation and Importance of Biodiversity (CIB), Ethics and Biodiversity (EB), Sustainability and Biodiversity (SB), Taking Action to Protect Biodiversity (TAPB), Biodiversity and Utility (BU), and Conservation and Importance of Species (CIS).  The method used was quantitative descriptive, involving 50 students as respondents. The instrument used consisted of 27 items that were analyzed using One Way ANOVA to determine the average score and significant differences based on gender and student scores. The results showed that the average score of biodiversity literacy obtained a score of 2.08 with a less significant category (Low Biodiversity Literacy). Most of the indicators are at an average value below 2.50, only a small number of indicators have an average value above 2.50. In the CIB dimension, only 3 indicators have an average value of > 2.50 out of 5 indicators. In the TAPB dimension, only 1 indicator has an average > 2.50. While in other dimensions, the average value < 2.50. In addition, there was no significant difference in student scores based on gender. Thus, student biodiversity literacy still needs to be improved, especially in the aspects of real action and individual responsibility.
Proteins Characterization in The Skin Mucus of Mutiara Catfish (Clarias Gariepinus) by Using SDS Page Electrophoresis Method Hanami, Cindy Claudea
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.14262

Abstract

Fish have a body defense system to protect themselves from various threats originating from the aquatic environment. One of the outermost defense mechanisms in the fish body is skin mucus. Mucus on fish skin is composed of water, glycoproteins, enzymes, antibodies, and other organic compounds. Through proteomic screening analysis, we can investigate the types and abundance of major proteins in fish mucosal exosomes. The aim of this study was to analyze the molecular weight of proteins in the surface mucus of mutiara catfish, and to identify the proteins detected in the mucus of mutiara catfish. The research method uses a descriptive method. The characterization process using SDS-PAGE. Analysis of protein molecular weight is obtained by measuring the displacement distance (Rf) in the sample. The molecular weight of proteins detected in mutiara catfish mucus had a molecular weight of 7.10 kDa, 13.51 kDa, 15.46 kDa, 28.78 kDa, 30.72 kDa, 42.40 kDa and 69.17 kDa. Proteins found abundantly in the mucus on the surface of catfish skin have a molecular weight ranging from 7-15 kDa, where this protein has various roles including immune defense, regulation of metabolism, oxidative stress, and the nervous system, stress regulation, regulation of cellular function, muscle contraction, and nervous system function. It can be concluded that the proteins found in the mucus on the surface of catfish skin are mostly small-sized proteins and have a major role in body defense, immune protection, and adaptation to environmental stress. These proteins are very important in maintaining fish health.

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