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Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Published by Universitas Insan Budi Utomo
ISSN : 2528679X     EISSN : 25979833     DOI : https://doi.org/10.33503/ebio.v9i02
Core Subject : Economy, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance
Edubiotik: Jurnal Pendidikan, Biologi dan Terapan aims to bridge the gap between research and practice, providing information, ideas, and opinions in biology research, teaching, and learning. Through coverage of policy and curriculum developments, the latest research results on biology teaching, learning, and assessment are brought to the surface. Edubiotik: Jurnal Pendidikan, Biologi dan Terapan accepts and publishes articles in the form of research in the fields of biology education, pure biology, and applied biology. All articles are published in English and undergo a peer-review process. The scope of Edubiotik: Jurnal Pendidikan, Biologi dan Terapan is focused on research in biology education, pure biology, and applied biology both in the topics discussed and the perspective of the discipline: 1. Biology teaching and learning materials at all levels of education; 2. Pure biology research developed or studied on sources, materials, or biology learning media (using the research and development/R&D paradigm); 3. Biology education curriculum at all levels of education; 4. Quasi-experiments, classroom action research (PTK), and Lesson Study in biology learning. Other qualitative research in biology education; 5. Evaluation/assessment of biology learning; 6. Professional issues/trends of teachers in biology education; 7. Environmental education; 8. All topics in applied biology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 163 Documents
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Assessment instruments for sustainability competences in applied biology: A systematic literature review Rahardjanto, Abdulkadir; Husamah, Husamah; Lestari, Nurdiyah; Hadi, Samsun; Permana, Tutut Indria
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.998

Abstract

Sustainability competences are essential for addressing complex global environmental, social, and economic challenges. However, tools to accurately assess these competences remain varied and underexplored, necessitating a comprehensive review of existing instruments. This study aims to systematically identify and analyze assessment instruments for sustainability competences through a systematic literature review (SLR). This type of research is a systematic literature review (SLR). The research instrument is an inclusion and exclusion criteria form. Using the Scopus database, articles published between 2010 and 2023 were screened based on inclusion criteria such as relevance to sustainability competences assessment, peer-reviewed status, and language (English). From an initial 234 articles, 11 met the criteria and were analyzed using bibliometric tools and qualitative synthesis guided by five research questions focusing on publication trends, subject areas, geographic distribution, keywords, and instrument characteristics. The results show dominance of Social Sciences in this field, with notable multidisciplinary inputs from Energy and Environmental Sciences. Key themes include teacher self-efficacy, institutional commitment, and educational tailoring, alongside challenges like weak theoretical foundations and limited curricular integration. The study concludes that culturally adapted and context-specific instruments are critical to effectively evaluate sustainability competences. It recommends further research to develop validated, comprehensive tools to support education and policy. This work provides a structured foundation for advancing sustainability competence assessment globally.
Mosquitoes' larval habitat characteristics and Aedes aegypti resistance status to malathion in Jember Nihayah, Husnatun; Purwatiningsih, Purwatiningsih; Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Puspitasari, Reni; Rohmah, Hidayatur
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1037

Abstract

Water-holding containers serve as breeding sites for Aedes aegypti. The control of mosquito breeding sites and the use of household insecticides are some of the DHF control methods. The use of household insecticides over a long period can reduce the susceptibility of mosquitoes to these insecticides. This research aims to determine the mosquito's habitat characteristics and to determine the Aedes aegypti resistance status to malathion in Jember.  A total of 61 houses were surveyed, and the various types of containers encountered at the sampling locations were recorded. Larvae and pupae found at the sampling location are brought and hatched in the laboratory. Aedes aegypti was tested for resistance status to malathion insecticide using a CDC bottle bioassay and biochemical nonspecific esterase enzyme activity. A total of 140 containers were found in this study, and 36.43% of them are Aedes aegypti larvae/pupae habitat. Types of containers used as mosquito larval habitat are bottles/glasses, bathtubs, used basins/buckets, water storage buckets, trash cans, refrigerator water containers, used cans, gutters, livestock drinking water containers, fish ponds, plant pots, used livestock cages, used toilets, used jugs, used animal feeders, and used aquariums. The mortality of Aedes aegypti reached 42.3% (resistant). Meanwhile, the results of biochemical tests showed an increase in the activity of the nonspecific esterase enzyme. This study concludes that Aedes aegypti from Jember has been resistant to malathion insecticide
Developing a classification of living things e-module with a jelajah alam sekitar approach to help students reduce misconceptions and strengthen scientific literacy Illah , Khofiatul; Amelia, Rizki Nor
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1224

Abstract

One strategy to reduce misconceptions and strengthen science literacy in the classification of living things is to facilitate students with contextual learning resources. Therefore, this study aims to develop an e-module as a learning resource that is integrated with the approach of Jelajah Alam Sekitar (JAS) so as to enable students to understand science concepts through collaborative practices of nature exploration. This research method is research and development using the 4D development model (define, design, develop, and disseminate). Class VII students at MTs Ishthifaiyah Nahdliyah were involved as samples with details of 180 students as the standardization test sample of the integrated science misconception-literacy instrument, 29 students as the e-module readability sample, and 32 students as the e-module implementation sample.  Data collection was carried out using interviews, a questionnaire, and student science literacy and misconception tests. The instruments used included interview guidelines, e-module characteristic questionnaires, e-module feasibility questionnaires, student response questionnaires, and pretest-posttest questions. Data analysis for the effectiveness of the e-module on student science literacy and misconceptions was performed using a paired t-test. The developed JAS E-module proved to be very feasible in terms of material concept, presentation, language, and graphics, and had excellent characteristics in terms of module characteristics, JAS components, and activities that contained indicators of misconceptions and science literacy. In addition, based on the results of the effectiveness test, in general, the e-module proved significant in reducing misconceptions (p<.05; t=-4.818; df=31) and strengthening students' science literacy (p<.05; t=4.636; df=31). Thus, this development contributes to improving science learning, especially in reducing misconceptions and strengthening science literacy.
Profile of flavonoid bioactive compounds in moringa leaves (Moringa oleifera) as phytoestrogens Primiani, Cicilia Novi; Pujiati , Pujiati; Prayitno, Trio Ageng; Setiawan, Mohammad Arfi; Rivera, Alice Tonido
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1446

Abstract

Moringa oleifera with the local name moringa, is widely used for health, as a medicinal plant. However, its potential as a natural estrogenic is not yet known. The study aimed to analyse and identify flavonoid compounds of moringa leaves and to analyse the estrogenic potential of moringa leaves on 17β-estradiol levels of female rats. Research with an experimental approach includes: 1) Metabolite profiling of moringa leaf extract using 95% methanol solvent, with stages including extract dilution, protein precipitation, and extract purification with SPE. Extract purification using solid phase extraction using C18 Sep-Pak; 2) Induction to 4-5 months female Wistar rats, 240 - 280 g, 24 heads, grouped into 3 treatment groups, namely control (P0), administration of 17β-estradiol (P1) and administration of 1.5 ml moringa leaf feeling water (P2), treatment for 25 days. Data collection techniques include LCMS chromatogram data and data on 17β-estradiol levels taken from rat tail veins. Descriptive data analysis techniques include: 1) Chromatogram result data of flavonoid compound groups and 2) Data on 17β-estradiol levels using ELIZA on day 26. The conclusion of the results showed that the flavonoid compounds of moringa leaves were 29 compounds, including isoflavone and coumestan groups. Giving moringa leaf juice can increase 17β-estradiol levels in rats by 524.346 mg/dl.
Improving students' scientific literacy through inquiry learning at Solo Safari Yuhanna, Wachidatul Linda; Retno, Raras Setyo
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1507

Abstract

The scientific literacy of students in the animal diversity course has not met the criteria. Students are less concerned, passive, and do not dare to express their problem-solving comprehensively. The animal diversity course requires learning methods and learning resources to improve scientific literacy. The purpose of this study was to analyze the effect of inquiry learning on improving students' scientific literacy at Solo Safari. This research method is quantitative research with a quasi-experimental method. The research design uses pre and post-test results of students’ scientific literacy. The study population was 71 biology education students. The sample was 13 students who took the Animal Diversity course. The data taken is students' scientific literacy. The independent variable is inquiry learning at Solo Safari. The dependent variable is science literacy. The instrument used is a science literacy instrument, resulting in 15 valid statement items. Data analysis techniques were carried out with a paired sample t-test. N-gain score analysis was conducted to determine the effectiveness of science literacy. The results showed that the sig-value (2-tailed) = 0.000. The inquiry learning model affects students' scientific literacy. The inquiry learning model can improve students' scientific literacy. The result of the N-gain score on science literacy is 0.62 with moderate criteria. There is a significant increase in understanding of science literacy after students take part in learning with the inquiry approach. The conclusion of this study is, inquiry affects improving students' scientific literacy at Solo Safari. The application of inquiry in Solo Safari can increase students' scientific literacy in the moderate category. The implications of this study can be used as basic research to choose the right learning model for animal diversity courses. Science literacy instruments can also be used for various biological studies and other materials.
A study of the impact of SETS-based multimedia assessment on students’ metacognitive abilities Hidayati, Nuril; Safitri, Nur Lina; Triyandana , Arga; Prayitno , Trio Ageng; Nisa, Khoirun; Riyanto, Riyanto
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1545

Abstract

There are difficulties in accustoming students to hone their metacognitive abilities in learning human anatomy and physiology in the era of the 4.0 revolution due to the lack of availability of SETS-based multimedia assessments. The purpose of this study was to analyze the effect of SETS-based multimedia assessments on students' metacognitive abilities. The research method was a quasi-experimental with a nonequivalent control group design pretest-posttest. The research sample was 2 classes (the experimental class was called A and the control class was called B) with 17 students in the experimental class, and 24 students in the control class in the Biology Education Study Program of IKIP Budi Utomo, who were taking the human anatomy and physiology course. The research instrument was pretest and posttest questions on the SETS-based multimedia assessment as many as 15 multiple-choice questions containing indicators of metacognitive abilities. The research data were in the form of pretest and posttest scores from the experimental class and the control class. The research data analysis technique used the Anacova test via SPSS for Windows. The results showed that SETS-based multimedia assessments affected students' metacognitive abilities (sig < 0.05). The study concludes that SETS-based multimedia assessment affects students' metacognitive abilities in human anatomy and physiology courses. The results of this study are recommended as assessment development materials to be integrated into the higher education curriculum as an effort to empower students' metacognitive abilities.
Lip balm formulation of onion dayak extract (Eleutherine palmifolia L.) and rosella flowers (Hibiscus sabdariffa Linn.) as a antioxidant and lip color Lestariningsih, Nanik; Ayatusa’adah, Ayatusa’adah; Maharani, Septia Puteri
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.876

Abstract

Plant extract contains active compounds beneficial for ultraviolet protection and skin health. No previous studies have formulated lip balm using Dayak onion and rosella flowers. The research aims to gain information on the best lipbalm formulation with parameters from panelists and preparation evaluation (pH test, homogeneity test, stability, smear test, and hedonic test). Experimental research method with completely randomized design (CRD), to identify the significant differences in the lip balm preparations using one-way ANOVA. Lip balm is formulated from an extract of dayak onion (0%,1%,3%,5%,7%) and rosella flower (0%,1%,3%,6%,9%) as a natural moisturizer and natural dye. The dayak onion bulbs and rosella flowers are extracted and tested for phytochemicals and DPPH antioxidants. The research results indicate that the extract of the dayak onion bulbs and rosella flowers are positive containing flavonoid, alkaloid, tannin, and triterpenoid compounds. The dayak onion bulbs have an IC50 value of 1,4366 ppm, whereas the IC50 for rosella flowers is 19,2135 ppm. The lipbalm preparation of F1, F2, F3, and F4 meet the requirements for pH testing, homogeneity test, smear test, and stability test. The results of the hedonic test on 35 panelists indicate that the most preferred color is in F3, texture in F1 and F3, and aroma in F1. The results of the one-way ANOVA test with a confidence level of 95% have a significance of 0,000 indicating a significant difference (P<0,05). The best extract formulation from dayak onion bulbs and rosella flowers in the lip balm innovative cosmetic herbal ingredients was in Formula 1 with extract concentration 1:1 and the best results of the color parameters according to the panelists were at F3.  
The effect of disinfectants on fertility of male mice (Mus musculus l.) Khofifah, Nur; Hanizar, Evi; Zuhro, Fatimatuz
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.987

Abstract

Since the outbreak of COVID-19, disinfectants have not only been used in health facilities, but they have also spread to households. The problem was that people often use disinfectant formulas that did not complied with health institutions guidelines. This study aims was to evaluate the effects of two types of disinfectants on the fertility, parameters of male mice, namely sperm concentration, motility and morphology. This was a true experimental study, with three treatment groups, namely the disinfectant type Alkyl Dimethyl Benzyl Ammonium Chloride (ADBAC) with a concentration of 10 mL/L, pine oil with a concentration of 30 mL/L, and control. The dose of each disinfectant was determined from the initial LD50 study. 20 mL of each disinfectant was sprayed on the cage twice a day in the absence of animals. The animals used were four week old male Mus musculus and were treated for 5 weeks. The parameters observed consisted of sperm concentration, motility, and morphology. All data were analyzed by one way Anova and Duncan's advanced test. The analysis results showed that the disinfectant had no effect on sperm concentration but instead had a negative effect on sperm motility and morphology. Both types of disinfectants reduced the progressive motility of sperm, while ADBAC produced more abnormal sperm morphology than pine oil. This condition is in accordance with other research that sperm motility is closely related to sperm morphology. The results of this study add to the evidence that disinfectants have a negative effect on male fertility parameters, namely sperm quality. The use of disinfectants for long periods also requires attention.  
Potential candidate probiotic Bacillus amyloliquefaciens strain NO10 SA8 in cholesterol assimilation and amino acid production Sukmawati; Hardianti , Fatimah; Amalia, Falina; Kaway, Sancy
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1038

Abstract

Probiotics are known as beneficial microorganisms that, when administered in appropriate amounts, can provide health benefits to their host. The ability of probiotics to produce amino acids is crucial to consider, as amino acids are essential components for the growth and development of organisms. The objective of this study is to analyze the ability of Bacillus amyloliquefaciens strain NO10 SA8 to assimilate cholesterol and its capability to produce amino acids. This study employed a descriptive method, including cholesterol assimilation and analysis of amino acid types produced by the potential probiotic bacterium Bacillus amyloliquefaciens strain NO10 SA8. The results of this study showed that Bacillus amyloliquefaciens strain NO10 SA8 ability demonstrated a relatively high cholesterol assimilation capacity—approximately 69.75% compared to the positive control. This indicates that the strain has potential to be used as a cholesterol-lowering agent in functional applications, such as probiotics or dietary supplements.it was capable of assimilating cholesterol. Furthermore, it was able to produce various amino acids, except for L-Histidine,  L-Tyrosine, and L-Tryptophan. The conclusion of this study is that Bacillus amyloliquefaciens strain NO10 SA8 has potential as a probiotic strain because it demonstrated the ability to assimilate cholesterol 69.75% compared to the positive control,  and producing amino acids.
Expression of the curculin gene from different organs of Curculigo latifolia under different culture conditions Muslihatin, Wirdhatul; Fajar, Nur Syamsiatul; Wibowo, Anjar Tri; Manuhara, Yosephine Sri Wulan
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1131

Abstract

Curculigo latifolia is the main source of curculin. Curculin is a special kind of protein that tastes sweet and can change the way food tastes, and is naturally expressed in fruit. This study aims to measure and compare the relative expression of the curculin gene in different organs, i.e., fruit and leaf, under in vitro and in vivo conditions. In this study, mRNA isolation was carried out in tissues derived from fruits and leaves grown in vitro and in vivo (in the soil) of C. latifolia from West Java, Indonesia. Leaves from 20 weeks seedling on polybag, fruit 40 days after anthesis, and leaves from 20 weeks seedling of C. latifolia cultured on MS0 medium that were used in this experiment. The relative expression was measured using qRT-PCR. The results showed that the comparison of curculigo’s parts in the leaves was lower than in the fruit. The lowest expression was obtained in leaves grown under in vitro conditions at 0.001-fold, while leaves grown in the soil at 0.566-fold compared to curculin gene expression in fruit. This study concludes that the expression of the Curculin gene in fruit is different between the fruit and leaves, and the growth conditions have an influence on Curculin gene expression, where Curculin gene expression under in vitro conditions is lower than expression under in vivo conditions. The curculin is not only expressed in the fruit but also the leaves, so it has the potential to be developed.

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