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Journal : Althea Medical Journal

Profile of Apoptotic Marker Genes and Histopathology of the Placenta in Pregnancies with Pre-Eclampsia Herlambang, Herlambang; Enis, Rina Nofri; Tarawifa, Susan; Harahap, Huntari; Puspasari, Anggelia; Maharani, Citra; Kusdiyah, Erny
Althea Medical Journal Vol 10, No 2 (2023)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v10n2.3088

Abstract

Background: Pre-eclampsia (PE) is a hypertensive disorder in pregnancy and a significant cause of maternal and perinatal mortality and morbidity. Failure of spiral artery remodeling due to abnormal apoptosis, triggers disturbances in the mother and the baby’s growth. This study aimed to identify the profile of apoptotic marker genes and histopathological features of the placenta in pregnancies with pre-eclampsia.Methods: This study had used case-control method. Samples were taken from normal pregnancies (n=25) and pregnant women with pre-eclampsia (n=25) using a purposive sampling method from several hospitals in Jambi. qRT-PCR was used to examine apoptotic gene expression from placental tissue and hematoxyline eosin staining to view the placenta’s microscopic appearance. The targeted genes were BCL2-associated X (BAX) and B-cell lymphoma 2 (BCL-2). Histopathological changes of the placenta observed were syncytial node, cytotrophoblast, villous edema, hypervascularization, fibrosis stroma, atherosis, infarction, and thrombosis.Results: Relative BAX genes expression were increased once in placenta pre-eclampsia compared to controls, but not statistically significant (p-value>0.05). There was no difference between the decline of BCL-2 gene expression in pre-eclampsia placenta compared to the control (p-value >0.05). Histopathological changes in the placenta were syncytial node and cytotrophoblast (25 of 25), villous edema (19 of 25), hypervascularization (24 of 25), fibrosis stroma (22 of 25), atherosis (12 of 25), infarction (17 of 25), and thrombosis (24 of 25).Conclusion: The expression of BAX genes in pre-eclampsia tends to increase compared to normal pregnancy, and the expression of BCL-2 decreases.  The histopathological features of pre-eclampsia pregnancy placenta are mostly syncytial nodes, cytotrophoblasts, stromal fibrosis, and thrombosis.
Co-Authors Ahmad Syauqy Ahmad Syauqy Amalya Nasution, Riska Amelia Dwi Fitri Anati Purwakanthi, Anati Andini Agustina Andini Agustina Anggelia Puspasari Annissa Delfira Anton Trihartanto Armaidi Darmawan Asro Hayani Harahap Asro Hayani Harahap Asro Hayani Harahap Asty, Zahra Frizki Attiya Istarini Betty Marlina Budi Aswin Citra Maharani Denok Tri Hardiningsih Devy Afriyanti Dia, Esa Indah Ayu Dwi Puspita, Puspa Eka Putra, Nofrans Ekaputri, Tia Wida Elfiani Elfiani Elfiani Elfiani Erina Shinta Anggraini Erni Kusdiyah Erny Kusdiyah Esa Indah Ayu, Esa Indah Esa Indah Ayudia Fadil Pramudya Firmansyah Firmansyah Firmansyah Fitriyanti Galih Saputra, Ikmal Hafizah Haifa Nida Firdausa Hanina Hanina, Hanina Hanina, Hanina Hasibuan, Mhd Usni Zamzami Herlambang HERLAMBANG HERLAMBANG Herlambang Herlambang Humaryanto, Humaryanto Humaryanto, Humaryanto Husnun Nisa Iksan, Pahrul Ilham Pratama Putra Imam, Mara indah ayu dia, Esa Indah Ayudia, Esa Indri Okta Sapitri Indrianti Irfannuddin Irfannuddin Irfanuddin Iskandar, Mirna Marhami JMJ, Jurnal Juniarti, Ariska Kurnia Justitia, Budi Krisna Murti Kusdiyah, Erni Lisa Susanti Maharini, Indri Mara Imam Taufiq Siregar Maria Estela Karolina Maria, Ima Martha Yosephine Renata Malau Meylani Mhd Usni Zamzami Hasibuan Miftahurrahmah Miftahurrahmah Miftahurrahmah, Miftahurrahmah Muhammad Anharuddin Muhammad Farhan Muhammad Yusri Nada Zahra, Risa Naufal Adli Marvi Nur, Amrizal Muhammad Nuriyah, Nuriyah Nyimas Natasha Ayu Shafira Nyndya Ariyanty Patrick William Gading Putra Agung, Rhonaz Putri Sari Wulandari Rina Nofri Enis Rion Nofrianda Rita Halim Sani K, Fathnur Subhan, Rahmatika Suzan, Raihanah Tarawifa, Susan Tengku Arief Buana Perkasa Tia Wida Ekaputri Ummi Kalsum Wahyu Indah Dewi Aurora Wida, Tia Willy Hardy Marpaung Zami, Muh. Husni Zam Zamzami , Mhd Usni Zulkhair Ali