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Journal : Jurnal Veteriner

Aktivitas Antibakteri Ekstrak Etanol dan Destilat Jahe dan Sirih terhadap Mycoplasma gallisepticum dan Escherichia coli (ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACT AND DESTILAT OF GINGERS AND PIPER BETLES AGAINST MYCOPLASMA GALLISEPTICUM AND ESCHERICHIA CO Min Rahminiwati; Aulia Andi Mustika; Agung Zaim; Lina Novianti Sutardi
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Betle and ginger juice extracts have been reported to have antibacterial activity against E. coliand M. gallisepticum. However, fractionation analysis showed the have differences in their antibacterialpotency which appeared to be associated with the nature of the solvent polarity. This study wasconducted to obtain information about antibacterial activity of Piper betles and gingers extract againstE. coli and M. gallisepticum. Three types of betle, P. betle, P. betle var nigra, P. crocatum, wereexamined for their antibacterial activity using disc method and three types of ginger, Zingiber officinale,Z. officinale Linn var rubrum, and Z. majus rumph which were extracted by soxhletation using ethanol30%, 60% and 90% as well as distestillation for three and five hours. Piper betle and P. crocatumconsistently have antibacterial effect against E. coli whereas Z. officinale consistently has antibacterialeffect against M. gallisepticum, either extracted by distillation or soxhletation. Piper betle is potentialyield of distillate and extract that has the highest antibacterial activity against E. coli, and M.gallisepticum with inhibitory zone were 9.76 mm and 22 mm respectively.
Induksi Ekstrak Pegagan Secara in vitro terhadap Proliferasi dan Diferensiasi Sel-Sel Otak Besar Anak Tikus (IN VITRO INDUCTION OF CENTELLA ASIATICA (PEGAGAN) EXTRACT ON THE PROLIFERATION AND DIFFERENTIATION OF NEWBORN RAT CORTEX CEREBRI CELLS) Ita Djuwita; Min Rahminiwati; Latifah Kosim Darusman; Siti Sa’diah
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to analyze the potency of Centella asiatica extract to induce proliferation andneurogenesis process of newborn rats cortex cerebri.   Research has been conducted on in vitro culture ofthree days old rat (Sprague Dawley) cerebrum cells in DMEM (Dulbecco’s Modiûed Eagle’s Medium)containing 10% NEAA (Non Essential Amino Acid), 1 mM NaHCO3, 10% NBCS (Newborn Calf Serum)and 50 µg/mL gentamycin (mDMEM), with and without Centella asiatica (CA) leaf extracts. The experimentwas set in five groups of treatment consisted of positive control (mDMEM+30 µg/mL asiaticoside (AC)),negative control (mDMEM), and mDMEM with three concentration of CA extract i.e. 100 ppm, 200 ppmand 400 ppm. Culture was done in 5% CO2 incubator at 37oC for six days. The parameters observed werecells proliferation based on Population Doubling Time (PDT), neuron and glia composition, and the lengthof axon and dendrite. Cells concentration were counted using Newbauer hemocytometer.  Neuron and gliacells were determined based on morphology after Hematoxylin-Eosin staining, and the length of axon anddendrite were measured using eyepiece micrometer. Data were analyzed using ANOVA and Duncan test.The results showed that Centella asiatica extract at concentration 100 ppm could induce neurogenesisand increased the axon length growth.  However, at concentration 200 and 400 ppm, CA extract  inhibitedthe neuronal cells proliferation and the axonal growth (P<0,05). In conclusion, induction of Centella asiaticaextracts at concentration of 100 ppm on the cortex cell cerebrum cells culture increase the axon lengthgrowth and tends to induce neurogenesis; however at higher concentration CA extract was neurotoxic.
Profil Kinetik dan Efektivitas Enrofloksasin yang Dikombinasikan dengan BioATP dalam Mengatasi Coxiella burnetii (KINETIC PROFILE AND EFFECTIVITY OF ENROFLOXACINE WITH BIO ADENOSIN TRIPHOSPHATE SUPPLEMENTATION AGAINST COXIELLA BURNETII) Andriyanto .; Agus Setiyono; Min Rahminiwati; Neni Nuryani; Unang Patriana
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Coxiella burnetii belongs to rikettsia group living obligate intracellularly and as the agent of zoonosisQ fever. Enrofloxacine is an antibiotic in quinolon group used to treat infection of C. burnetii in chicken,goat, calve, pig, dog, cat,  and horse. From ruminant practical experience, enrofloxacine if combined withBioATP  can enhance the enrofloxacine activity. Research for the effecivity of enrofloxacine and BioATP totreat C. burnetii has never been carried out. The research was conducted to explore effect of enrofloxacinewith supplementation BioATP against C. burnetii. Enrofloxacine pharmacokinetic study was carried outby using simental beef as an experimental animals. The effectivity of BioATP supplementation onenrofloxacine activity to treat C. burnetii was tested by using Vero cell tissue culture. The results showedthat combination of enrofloxacine and BioATP increased kinetic profile of enrofloxacine in term of onset,duration, pharmacology intensity, and bioavailaibility. Enrofloxacine had activity to treat C. burnetii withvalue of minimal inhibitory concentration (MIC) at 1-2 ppm and value of minimal bactericidal concentrationat 4 ppm. Supplementation of BioATP improved the effectivity of enrofloxacine in treating C. burnetii.
Gabungan Ekstrak Rimpang Temulawak, Daun Tanjung, dan Daun Belimbing Manis Berdasarkan Electrocardiogram Berpotensi Sebagai Antiaritmia pada Kucing Min Rahminiwati; Widia Safitri; Deni Noviana
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.377 KB) | DOI: 10.19087/jveteriner.2019.20.3.409

Abstract

Mimusops elengi L. , Averrhoa carambola L. and Curcuma xanthorrihiza Roxb singly were reported to have hypothensif effect. Mechanism underlying decrease of blood pressure was suggested through modification of cardiac activity. The Effect of Mimusops elengi L., combined with Averrhoa carambola L. and Curcuma xanthorrihiza Roxb extract on cardiac activity were studied using ECG on 12 male cats that were grouped to be a control group administered aquadestilata, and treatment group administered extract of 21 mg and 82 mg/2 kg bw orally respectively. The extracts were given 3 h prior to ECG. The results showed a decrease in P wave, QRS complex and the speed of the heart rate after administration of combination of extract . However QT and PR intervals were increased. This showed that the extract can weaken a contraction of the Atria and ventricles, prolonging the onset of the occurrence of atrial contraction towards ventricular contraction, extending the onset of contraction and relaxation of the ventricles and cause a decrease in heart rate. Based the cardiogram, It was concluded that their combination is valuable for treatment of aritmia.
Studi Karakteristik Tipe Diabetes pada Tikus (Rattus novergicus) yang Diinduksi Deksametason (CHARACTERISTIC STUDY OF THE TYPE OF DIABETES IN RAT (RATTUS NOVERGICUS) MODEL INDUCED BY DEXAMETHASONE) Rahmania Hanim; Huda Shalahudin Darusman; Min Rahminiwati
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.21 KB) | DOI: 10.19087/jveteriner.2018.19.1.1

Abstract

Diabetes is marked by hyperglicemia with severe symptoms included polydipsi, polyuri, polyphagia,weight loss, and decreased of insulin sensitivity index specially at type 2 diabetes mellitus. The objective of this research was to analyze the characteristics of diabetes mellitus in rat (Rattus novergicus) which induced by dexamethason. A total of 24 rats were divided into four groups which divided as follow: (1) aquabidest subcutaneously (K0), (2) subcutaneous dexamethasone 1 mg/kgBW daily (K1), (3) subcutaneous dexamethasone 2.5 mg/kgBW daily (K2) and subcutaneous dexamethasone 5 mg/kgBW daily (K3). The data of fasting blood glucose, fasting blood insulin, body weight and feed intake, urine volume and water intake collected at day 0, 5, 10 and 15 (five days after the last treatment). The result showed that there was a significant elevation of glucose level followed by significant decreased of insulin sensitivity index and the body weight of rats in group wich induced by dexamethasone but there was no change in feed intake, water intake, volume of urine and fasting blood insulin before and after the treatment. There was insignificant different of total beta pancreatic islet between treatment and control grup. Induction of dexamethasone in mice causes reversible hyperglycemia and has not met the characterisics of either type 1 or 2 diabetes mellitus models. The profile of dexamethasone induced diabetes model has their own spesific type.
Co-Authors , Triadiati . andriyanto . Soeripto A.S. Satyaningtijas Achmad Tjahja Ade Sunarma Ade Sunarma Adi Riyadhi Agik Suprayogi Agus Setiyono Ahmad Subhan Ahmad Tjahja Nugraha, Ahmad Tjahja Amrozi Andriyanto . Andriyanto A Andriyanto Andriyanto Anggraena, Shinta Suci Ani Murtisari April Hari Wardhana Arief Boediono Aryani Satyaningtijas Aswani, Tuti Aulia Andi Mustika Bambang Pontjo Priosoeryanto Budi Wirawan Cantika Zaddana Ceristianto Ceristianto Ceristianto, Ceristianto Chudahman Manan Chudahman Manan Daldiyono Daldiyono Daldiyono Daldiyono Damiana Rita Ekastuti Dede Sukandar Deni Noviana Djumali Mangunwidjaja Dyah Iswantini E Mulyati Effendi, E Mulyati Erni Rustiani Fadillah, Wendi Nurul Fajar Triansyah Gumelar Firman, Gelline Tama Anindia Fitri Dwiputri Franco, Christopher MM Gelline Tama Anindia Firman Gumelar, Fajar Triansyah Hajar Hajar Hambarsika, IGM Antara HANDAJANI, FITRI Hidayanti, Tiyan Huda Shalahudin Darusman IETJE WIENTARSIH Ika Amalia, Ika Ike Yulia Wiendarlina Indriani, Lusi Irmanida Batubara Ita Djuwita Ita Juwita Joshie Ramadhan Juwita, Ita Kusdiantoro Mohamad La Ode Sumarlin La Ode Sumarlin latifah K Darusman latifah K Darusman Latifah Kosim Darusman Lina Novianti Sutardi Livana Dethris Rawung M. Agung Zaim Adzkiya M. Zairin Junior Mahyuni, Siti Martha Arofa Dilla Mashuri Waite Meyliana Wulandari MUHAMMAD AGUS SUPRAYUDI Murtisari, Ani Mutiara, Tia Najwa Rokhmah, Nisa Nampiah Sukarno Neni Nuryani Nhestricia, Nhadira Novriyandi Hanif Oom Komala Rahmania Hanim Rahmat Hidayat Raudhatul Jannah Rosihan Rosman SAEPUDIN RAHMATULLAH Sara Nurmala Shinta Suci Anggraena Sholehah, Akipah Nazla Siti Saadah Siti Sa’diah Sri Budiarti Poerwanto Sri Estuningsih Sri Nuryati Suci Sintya Sulistyaningsih, Yohana Caecilia SUMINAR S. ACHMADI Supardi, Arviani Rahmawati Tia Mutiara Tuti Aswani Ulandari, Rafika Unang P. Unang Patriana Upik Kesumawati Hadi Waite, Mashuri Wasmen Manalu Wei Zhang Wendi Nurul Fadillah Wida Lesmanawati Widia Safitri Yasinta Ratna Esti Wulandari Yohana Caecilia Sulistyaningsih YULIN LESTARI Zaenal Abidin