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All Journal Sainteks Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Jurnal Akuakultur Sungai dan Danau Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Pengabdian Magister Pendidikan IPA Aquacultura Indonesiana Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Jurnal Perikanan Pantura Proceedings Series on Physical & Formal Sciences Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Jurnal MAIYAH Molekul: Jurnal Ilmiah Kimia Jurnal Perikanan Artha Imperium: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Multidisciplinary Science Arwana: Jurnal Ilmiah Program Studi Perairan
Syakuri, Hamdan
Fakultas Perikanan Dan Ilmu Kelautan, Universitas Jenderal Soedirman
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Inventory of ectoparasites and molecular identification of pathogenic bacteria on bonylip barb (Osteochilus vittatus) cultivated in Banyumas District Anandita Ekasanti; Hamdan Syakuri; Dewi Nugrayani; Emyliana Listiowati; Muhammad Nurhafid Nurhafid; Alvin Maulana
Arwana: Jurnal Ilmiah Program Studi Perairan Vol 5 No 1: Mei 2023
Publisher : Program Studi Akuakultur, Fakultas Pertanian, Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/jipsbp.v5i1.1852

Abstract

The aims were to do an ectoparasite and pathogenic bacteria inventory of Bonylip barb reared in Banyumas district. Total of 118 fish were randomly taken from Singasari (30 individual), Pabuaran (30 individual), Beji (30 individual) and Jipang (28 individual) villages. This work observed ectoparasite species, prevalence, and intensity as well as pathogenic bacteria species. Ectoparasites were identified based on morphological characteristics observed microscopically and their prevalence and intensity were calculated. Bacterial pathogen was isolated from fish samples showing symptoms of bacterial disease and identified molecularly based on 16s rDNA sequence using BLAST dan phylogenetic analysis. Results showed Trichodina sp. and Monogeneans were found infected fish samples from all location with prevalence was 70-100%. Ichthyophthirius multifiliis was found from all location except Singasari village.  Some of fish samples showed fin necrosis and five bacterial isolates were obtained. 16s rDNA marked gene was successfully amplified and sequenced. Based on sequence analysis, five isolates could be clustered into two groups, the first was found only in Singasari village and the second was found in Singasari and Beji villages.  Similarity value of 16s rDNA gene sequence these two groups was 99,8%. Result of BLAST and phylogenetic analysis showed that the bacterial pathogens were identified as group of Aeromonas hydrophila complex.
Co-Authors A. Ekasanti Ade Rusman Agung Cahyo Setiawan Agung Cahyo Setyawan Agung Cahyo Setyawan Agus Nuryanto Ahmad Hanif Pranoto Utomo ALIM ISNANSETYO Alvin Maulana Astuti - Aueresta Gita Syahbila D. Nugrayani D. Wisudyanti Dewi Fitrya Nugraheny Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Wisudyanti Dewi Wisudyanti Budi Hastuti Dyahruri Sanjayasari Ekasanti, Anandita Emyliana listiowati Emyliana Listiowati Emyliana Listiowati Emyliana Listiowati Emyliana Listiowati, Emyliana Emyliana Listyowati Endang Hilmi Endang Hilmi Faizah, Farkhah Nur Hary Tjahja Soedibya Hendro Pramono Ikhsan Pratama Imlami, Ainulyakin Hasan INDAH ISTIQOMAH Ismail, Norshida Iswardhani Ariyanti Jefri Anjaini Junaidi, Teuku K. H. Nitimulyo Kasprijo, Kasprijo Kusuma, Baruna Kusuma, Rima Oktavia Latifah, Ma'rufa Nurul Maeda, Minoru Marhaendro Santoso Mas Yedi Sumaryadi Mohammad Nurhafid Muh. Sulaiman Dadiono Muhammad Nurhafid Muhammad Nurhafid Nurhafid Murti, Fauziah Arelia Usmiara Murwantoko . Muslih Muslih Muslih Muslih Ningam Syukri Norman Arie Prayogo Norman Arie Prayogo, Norman Arie Nugrayani, Dewi Nuning Vita Hidayati Nur Wijayanti Nurchamidah Oedjijono Oedjijono P.Hary Tjahja Palupi, Mustika Philipus Uli Basa Hutabarat Prastiyo, Jery Purnama Sukardi Purnama Sukardi Purnama Sukardi Purwandaru Widyasunu R. Oktavia Rose Dewi Rosita, Rita Eka Rusman, Ade Senny Helmiati Setyawan, Agung Cahyo Slamet Budi Prayitno Soedibya, Petrus Hary Tjahja Sofi Khoerun Nisa Sri Marnani Sri Marnani Sri Marnani Steinhagen, Dieter Syahbila, Aueresta Gita Syukri, Ningam Taufik Budhi Pramono Taufik Budhi Pramono Taufik Budhi Pramono Tjahja, P.Hary Tohap Simangunsong Triyanto Triyanto Undiono Undiono Vaediyasari, Dahniar Wibowo, Satrio Haryu Widhiatmoko Herry Purnomo Wijaya, Rudy Yohanes Evander