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Optimalisasi Pemanfaatan Lahan dan Sumber Air melalui Kombinasi Budidaya Dalam Ember dan Akuaponik untuk Peningkatan Gizi Masyarakat Desa Serayu Larangan, Kabupaten Purbalingga Emyliana Listyowati; Anjaini, Jefri; Hamdan Syakuri; Anandita Ekasanti; Dewi Nugrayani; Tohap Simangunsong; Nurchamidah; Widhiatmoko Herry Purnomo
Jurnal Pengabdian Magister Pendidikan IPA Vol 7 No 4 (2024): Oktober-Desember 2024
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v7i4.9823

Abstract

Budikdamber (fish farming in buckets) and aquaponics are integrated farming technologies that integrate catfish with kale and mustard greens. catfish with kale and mustard greens. This system is implemented in Serayu Larangan Village, Purbalingga Regency, as an effort to improve food security and sustainable land utilization. and sustainable land utilization. The implementation of this program showed positive results, with optimal catfish growth and productive kale and mustard greens harvests. kale and mustard greens are productive in a short time. This integration utilizes fish organic waste as nutrients for plants, thus creating a mutually supportive ecosystem. creating a mutually supportive ecosystem. The success of this program opens up potential for the development of Budikdamber and aquaponics as a business opportunity for the Serayu Larangan Village community. In addition to supporting household food security household food security, this system offers an economical and environmentally friendly business alternative. environment. Based on the results of the implementation, the Budikdamber and aquaponics program can be concluded as a successful and innovative step. program can be concluded as a successful innovative step that can be adapted to other areas with similar conditions. adapted for other areas with similar conditions. This program is an example of application of integrated cultivation technology that is efficient and sustainable
The Impact of Climate Changes on Primary Producer of Aquatic Ecosystem (Phytoplankton) in the Ocean Hutabarat, Philipus Uli Basa; Sanjayasari, Dyahruri; Syakuri, Hamdan
MAIYAH Vol 3 No 4 (2024): Maiyah : Vol. 3 No. 4 Desember 2024
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.maiyah.2024.3.4.13948

Abstract

Aquatic ecosystems are very important for biodiversity and play a crucial role for the environment as well as for humans. Phytoplankton, the primary producers in this system, significantly contribute to oxygen production and CO2 absorption, making them crucial for global climate regulation. However, climate change marked by rising temperatures and unpredictable weather patterns has negatively impacted the abundance and distribution of phytoplankton, thereby threatening marine biodiversity and the stability of ecosystems. This study focuses on analyzing the impact of climate change on the abundance of phytoplankton in the oceans and identifying strategies to address it, through literature review. The research results indicate a significant decline in phytoplankton abundance, influenced by significant temperature changes from year to year. Effective mitigation strategies, including efforts to reduce greenhouse gases, protect biodiversity, and educate the public, are essential for preserving aquatic ecosystems. This research emphasizes the need for immediate and sustained action to protect marine biodiversity and ensure ecological balance.
Aplikasi Budidaya Ikan Dalam Ember (Budikdamber) Di Kelompok PKK Desa Sawangan Wetan, Kecamatan Patikraja, Kabupaten Banyumas ekasanti, anandita; Dewi Nugrayani; Emyliana Listiowati; Dewi Wisudyanti Budi Hastuti; Hamdan Syakuri
Artha Imperium: Jurnal Pengabdian Kepada Masyarakat Vol. 2 No. 2 (2024): 2024
Publisher : PT. Artha Genetikalab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/fzhndj15

Abstract

Budidaya ikan dilakukan dengan menggunakan ember-emberyang dirancang untuk memelihara ikan sekaligus sayuransecara bersamaan. Tujuan kegiatan ini adalah memberikanpengetahuan dan keterampilan memelihara ikan dan sayurandalam ember kepada ibu-ibu kelompok PKK. Metode kegiatanyang digunakan adalah belajar sambil bekerja (learning bydoing). Pelaksanaan kegiatan dengan model pembelajaranorang dewasa (andragogik) dan partisipatif dalam empattahapan yang meliputi alih teknologi melaluisosialisasi/penyuluhan aplikasi budidaya ikan dalam ember,pelatihan dan percontohan, pendampingan, dan evaluasikegiatan. Hasil kegiatan menunjukkan bahwa 94,44% ibu-ibuPKK peserta pelatihan telah mengetahui teknik budidaya ikanlele dan kangkung dalam ember serta mampu untukmengaplikasikannya dengan baik. Setelah 4 minggupemeliharaan, tinggi tanaman kangkung berkisar 18-22 cmdan sudah bisa dipanen. Setelah 6 minggu pemeliharaan,berat ikan lele mencapai kisaran 38,33-55,33 gram dengantingkat kelangsungan hidup berkisar 80-85%.
The Impact of Ectoparasites on Morphometric Profiles of Hotate Scallop In Funka Bay, Hokkaido, Japan Faizah, Farkhah Nur; Syakuri, Hamdan; Sanjayasari, Dyahruri; Imlami, Ainulyakin Hasan
MAIYAH Vol 4 No 2 (2025): Maiyah : Vol. 4 No 2 Juni 2025
Publisher : Fakultas Perikanan dan Ilmu Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.maiyah.2025.4.2.16685

Abstract

The hotate clam (Mizuhopecten yessoensis) is one of the clam species that lives in cold-temperature waters with the largest production in the Japanese region. However, the sustainability of this aquaculture is threatened by various factors, including ectoparasite infections that can affect the morphometric profile of the scallop. This study aimed to analyze the impact of ectoparasites on the morphometry of hotate clams in Funka Bay, Hokkaido, and to inventory the types of ectoparasites found. Samples of 25 individual hotate mussels were taken using random sampling techniques and morphometric measurements were taken using Image-J software. Ectoparasite infestation was categorized into four categories: free, low, medium and high. Analysis was conducted using One-Way ANOVA and Fisher's Pairwise Comparison follow-up test (P<0.05), as well as hierarchical cluster analysis based on the Bray-curtis similarity index visualized in a dendrogram. The results showed that there were two types of ectoparasites, namely Polydora sp. and Barnacles sp., with the dominance of Polydora sp. which was found in 17 individuals. Some morphometric distances such as AD, AE, BD, and CE showed significant differences between infestation categories. The dendrogram showed that the 'free', 'low', and 'medium' categories had high morphometric similarity (0.987), while the 'high' category showed lower similarity (0.96).
GROWTH PERFORMANCE AND INTESTINAL Aeromonas spp. OF BONYLIP BARB (Osteochilus vittatus) AFTER RECEIVING DIET CONTAINING SALT AND HERBAL CONCOCTION SUPPLEMENTATION Syakuri, Hamdan; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Ekasanti, Anandita; Kasprijo, Kasprijo; Kusuma, Rima Oktavia; Wijaya, Rudy; Nugrayani, Dewi; Listiowati, Emyliana; Palupi, Mustika
Indonesian Aquaculture Journal Vol 19, No 2 (2024): (December, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.2.2024.111-122

Abstract

Salt and herbal supplementations in fish feed are expected to promote aquaculture productivity, including for slow-growing fish such as Bonylip barb (Osteochilus vittatus). The objective of this study was to evaluate the growth performance and occurrence of Aeromonas spp. in the intestine of Bonylip barb fed a diet supplemented with salt and herbs. This experiment was conducted using a completely randomized design with four treatments: 1) control, 2) salt 3% supplementation, 3) herbal supplementation, and 4) Salt 3% and herbal supplementation. Growth performance evaluation included weight gain, relative growth rate, and specific growth rate after 30 and 60 days of rearing.  Bacterial samples were isolated on trypticase soy agar (TSA) and glutamate starch phenol red agar (GSP) media. Yellow colonies on GSP were counted and confirmed as Aeromonas spp. via molecular identification based on 16S rDNA sequence. Results showed that herbal supplementation, either alone or in combination with 3% salt, insignificantly increased the fish growth performance in the short-term (30 days), but tended to decrease it over the long-term (60 days). Salt supplementation consistently decreased fish growth performance but improved fish survival rates. Aeromonas isolates comprised more than 60 % of the culturable intestinal bacteria in all treatments. Furthermore, herbal supplementation appeared to increase the percentage of Aeromonas spp. in the intestine, with identified species including Aeromonas veronii, Aeromonas caviae, and Aeromonas jandaei.
ISOLASI, IDENTIFIKASI, DAN ANALISIS EKSPRESI GEN PENGKODE GROWTH HORMONE PADA IKAN SIDAT (Anguilla bicolor) Syakuri, Hamdan; Wibowo, Satrio Haryu; Ekasanti, Anandita; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Listiowati, Emyliana; Nugrayani, Dewi; Sukardi, Purnama
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.69-83

Abstract

Gen hormon pertumbuhan (growth hormone, GH) perlu dipelajari untuk mendukung domestikasi ikan sidat Anguilla bicolor di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi gen pengkode GH serta menganalisis ekspresinya pada sampel ikan sidat A. bicolor. Primer untuk amplifikasi gen GH ikan sidat A. bicolor didesain berdasarkan sekuen gen GH dari beberapa spesies ikan sidat lain yang tersedia di GenBank. Sampel cDNA hipofisa dan otak ikan sidat digunakan untuk amplifikasi gen GH. Hasil amplifikasi disekuensing dan hasilnya dianalisis menggunakan analisis BLAST, multiple sequences alignment, hormon signature, dan filogenetik. Analisis ekspresi gen GH dilakukan menggunakan teknik realtime PCR dengan metode delta-delta CT pada 14 sampel ikan sidat (23,1 ± 19,6 g; 24,5 ± 4,2 cm). Fragmen DNA sepanjang 486 bp berhasil diamplifikasi dan disekuensing. Sekuen gen GH A. bicolor memiliki similaritas nukleotida sebesar 98,49-99,14% jika dibandingkan dengan gen GH ikan sidat A. anguilla, A. australis, dan A. japonica. Sekuen parsial tersebut secara in silico diketahui mengkode bagian dari protein GH sepanjang 155 asam amino (aa). Sekuen asam amino protein GH A. bicolor sangat mirip dengan sekuen spesies ikan sidat lain dengan hanya tiga perbedaan asam amino dan membentuk satu percabangan pada pohon filogenetik. Tingkat ekspresi gen GH pada sampel ikan sidat memiliki variasi yang tinggi. Hasil penelitian ini dapat menjadi dasar untuk studi selanjutnya khususnya berkaitan dengan peran gen GH dalam pertumbuhan ikan sidat A. bicolor.The growth hormone (GH) gene needs to be studied to support the domestication of the Anguilla bicolor eel in Indonesia. This study aimed to isolate and identify the GH coding gene and analyze its expression in samples of the eel A. bicolor. Primers for amplification of the GH gene of A. bicolor eels were designed based on GH gene sequences from several other eel species available in GenBank. Pituitary and brain cDNA samples of eel were used for GH gene amplification. The amplification results were sequenced and the results were analyzed using BLAST analysis, multiple sequence alignment, hormone signature, and phylogenetic analysis. The GH gene expression analysis was carried out using the real-time PCR technique with the delta-delta CT method on 14 eel samples (23.1 ± 19.6 g; 24.5 ± 4.2 cm). The 486 bp DNA fragment was successfully amplified and sequenced. The GH gene sequence of A. bicolor has a nucleotide similarity of 98.49-99.14% when compared with the GH gene of other eel species A. anguilla, A. australis, and A. japonica. This partial sequence was found in silico to code 155 amino acids (aa) GH protein. The amino acid sequence of the A. bicolor GH protein is very similar to that of other eel species with only three amino acid differences and forms one branch on the phylogenetic tree. The expression level of the GH gene in eel samples had high variations. The results of this study could be a basis for further studies, especially regarding the role of the GH gene in the growth of the eel A. bicolor. 
PENAPISAN BAKTERI SELULOLITIK PADA SALURAN PENCERNAAN IKAN KERAPU CANTANG YANG DIBUDIDAYAKAN DI DESA BABAKAN, KECAMATAN PANGANDARAN, KABUPATEN PANGANDARAN Syukri, Ningam; ., Kasprijo; Tjahja, P.Hary; Syakuri, Hamdan; Listiowati, Emyliana
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 9 No 2 (2021): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.946 KB) | DOI: 10.29406/jr.v9i2.3000

Abstract

Ikan Kerapu Cantang merupakan hasil pesilangan dari ikan Kerapu Macan betina dan ikan Kerapu Kertang jantan. Ikan Kerapu Cantang merupakan salah satu komoditas perikanan budidaya yang memiliki nilai ekonomis tinggi. Penelitian ini bertujuan untuk mengetahui keberadaan bakteri selulolitik serta aktivitas bakteri selulolitik pada saluran pencernaan ikan Kerapu Cantang. Metode yang digunakan pada penelitian ini adalah metode observasi dengan pengambilan sampel secara purpose random sampling. Variabel utama yang diamati pada penelitian ini yaitu mengamati proporsi bakteri selulolitik dan aktivitas bakteri selulolitik pada saluran pencernaan ikan Kerapu Cantang. Untuk variabel pendukung pada penilitian ini yaitu kelimpahan bakteri, uji gram KOH, proporsi Bacillus Serta pewarnaan gram. Keberadaan bakteri Selulolitik pada saluran pencernaan ikan Kerapu Cantang pada penelitian ini ditunjukkan dengan adanya zona bening di sekeliling koloni bakteri yang ditumbuhkan pada media spesifik ( CMC 1%). Hasil penelitian ini untuk proporsi keberadaan bakteri selulolitik pada bagian anterior 49,33% middle 38,66% dan posterior 28%. Aktivitas bakteri selulolitik dari penelitian ini menunjukan hasil dengan indeks hidrolisis pada bagian anterior sebesar 0,14 – 1,4, midddle 0,1 – 3,3 dan posterior 0,2 – 1,6.
Modulation of Gut Microbiota, Nutrient Transport Gene Expression, and Growth of Red Hybrid Tilapia (Oreochromis sp.) Fed with Black Soldier Fly (Hermetia illucens) by Oral Probiotics Latifah, Ma'rufa Nurul; Murti, Fauziah Arelia Usmiara; Helmiati, Senny; Ismail, Norshida; Syakuri, Hamdan; Istiqomah, Indah; Murwantoko, Murwantoko; Isnansetyo, Alim
Jurnal Perikanan Universitas Gadjah Mada Vol 26, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.95268

Abstract

Maggot Black Soldier Fly (BSF) is a nutritionally rich alternative food for fish. The slow digestibility requires the combination with other technologies to produce fish with excellent growth. This study aims to investigate the impact of probiotic application on the growth, microbiome, and gene expression in the intestines of tilapia-fed black soldier fly (BSF) larvae. Tilapia intestines were examined after two months of rearing with three replications and two treatments: A) 30% maggots and 70% commercial pellets with probiotics, and B) 30% maggots and 70% commercial pellets without probiotics. We discovered that probiotics have a significant impact on the gut microbiomes of fish and their absolute growth. The amplicon sequence variant in the probiotic treatment (A) was 25, with dominance by Cetobacterium, Acinetobacter, Enhydrobacter, and Gemmobacter, while the non-probiotic treatment (B) was 8, with dominance by Cetobacterium and Turicibacter. The probiotic treatment increased the expression of Ghrelin, Muc-2, IL-1β, and I-FABP genes, but not the CD36 gene. These findings suggest oral probiotics can help boost tilapia production when fed maggot black soldier fly (Hermetia illucens).
Inventory of ectoparasites and molecular identification of pathogenic bacteria on bonylip barb (Osteochilus vittatus) cultivated in Banyumas District Anandita Ekasanti; Hamdan Syakuri; Dewi Nugrayani; Emyliana Listiowati; Muhammad Nurhafid Nurhafid; Alvin Maulana
Arwana: Jurnal Ilmiah Program Studi Perairan Vol 5 No 1: Mei 2023
Publisher : Program Studi Akuakultur, Fakultas Pertanian, Universitas Almuslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/jipsbp.v5i1.1852

Abstract

The aims were to do an ectoparasite and pathogenic bacteria inventory of Bonylip barb reared in Banyumas district. Total of 118 fish were randomly taken from Singasari (30 individual), Pabuaran (30 individual), Beji (30 individual) and Jipang (28 individual) villages. This work observed ectoparasite species, prevalence, and intensity as well as pathogenic bacteria species. Ectoparasites were identified based on morphological characteristics observed microscopically and their prevalence and intensity were calculated. Bacterial pathogen was isolated from fish samples showing symptoms of bacterial disease and identified molecularly based on 16s rDNA sequence using BLAST dan phylogenetic analysis. Results showed Trichodina sp. and Monogeneans were found infected fish samples from all location with prevalence was 70-100%. Ichthyophthirius multifiliis was found from all location except Singasari village.  Some of fish samples showed fin necrosis and five bacterial isolates were obtained. 16s rDNA marked gene was successfully amplified and sequenced. Based on sequence analysis, five isolates could be clustered into two groups, the first was found only in Singasari village and the second was found in Singasari and Beji villages.  Similarity value of 16s rDNA gene sequence these two groups was 99,8%. Result of BLAST and phylogenetic analysis showed that the bacterial pathogens were identified as group of Aeromonas hydrophila complex.
Sosialisasi Pembuatan Pelet Ikan Berbasis Magot sebagai Upaya Peningkatan Kemandirian Pakan di BUMDes Hardjo Utomo Purnama Sukardi; Lilik setiyaningsih; Anandita Ekasanti; Baruna Kusuma; Hamdan Syakuri; Hanisya Putri Kania Mardika; Asro Nurhabib; Frentina Murti Sujadi; Jefri Anjaini; Moch Riziq Imanulhaq; Tika Widiastuti; Irfan Hanifa; Yohanes Harvinda; Hana
Jurnal Pengabdian Magister Pendidikan IPA Vol 9 No 3 (2026): Juli-September 2026
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v9i3.16031

Abstract

High feed costs are a major constraint in aquaculture, accounting for more than 60% of total production expenses. This challenge is experienced by BUMDes Hardjo Utomo in Sukoharjo Village, Wonosobo Regency, which still relies heavily on commercial fish feed, highlighting the need for more economical and sustainable feed alternatives. This community service program aimed to enhance the knowledge and skills of local communities regarding the utilization of Black Soldier Fly (BSF) larvae as a raw material for fish pellets to support feed self-sufficiency. The program was implemented through socialization, educational counseling, interactive discussions, demonstrations of maggot-based fish pellet production, and evaluation through interviews and final discussions. The materials covered the nutritional potential of BSF larvae, feed formulation, ingredient processing, pellet production, drying techniques, and feed quality standardization. The results showed that participants responded positively and demonstrated improved understanding of the potential of BSF larvae as an alternative protein source, fish pellet production techniques, and the importance of feed quality in supporting fish growth. Hands-on demonstrations proved more effective in enhancing participants' understanding than theoretical presentations alone. In addition, the program increased community awareness of the economic and environmental benefits of utilizing organic waste as a substrate for BSF cultivation. The management of BUMDes expressed interest in developing a maggot-based pellet production unit as part of strengthening village aquaculture enterprises. However, further assistance is still required, particularly in feed quality standardization, equipment operation, and business management. Overall, the program successfully strengthened community capacity to utilize local resources in supporting fish feed self-sufficiency, reducing dependence on commercial feed, and promoting sustainable rural economic development.
Co-Authors A. Ekasanti Ade Rusman Agung Cahyo Setiawan Agung Cahyo Setyawan Agus Nuryanto Ahmad Hanif Pranoto Utomo ALIM ISNANSETYO Alvin Maulana D. Nugrayani D. Wisudyanti Dewi Fitrya Nugraheny Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Nugrayani Dewi Wisudyanti Dewi Wisudyanti Budi Hastuti Dyahruri Sanjayasari Ekasanti, Anandita Emyliana Listiowati Emyliana Listiowati Emyliana Listiowati Emyliana listiowati Emyliana Listiowati, Emyliana Emyliana Listyowati Endang Hilmi Endang Hilmi Faizah, Farkhah Nur Frentina Murti Sujadi Hana Hanisya Putri Kania Mardika Hary Tjahja Soedibya Hendro Pramono Ikhsan Pratama Imlami, Ainulyakin Hasan INDAH ISTIQOMAH Irfan Hanifa Ismail, Norshida Iswardhani Ariyanti Jefri Anjaini Junaidi, Teuku K. H. Nitimulyo Kasprijo, Kasprijo Kusuma, Baruna Kusuma, Rima Oktavia Latifah, Ma'rufa Nurul Lilik setiyaningsih Marhaendro Santoso Mas Yedi Sumaryadi Moch Riziq Imanulhaq Mohammad Nurhafid Muh. Sulaiman Dadiono Muhammad Nurhafid Muhammad Nurhafid Nurhafid Murti, Fauziah Arelia Usmiara Murwantoko . Muslih Muslih Muslih Muslih Ningam Syukri Norman Arie Prayogo Norman Arie Prayogo, Norman Arie Nugrayani, Dewi Nuning Vita Hidayati Nur Wijayanti Nurchamidah Oedjijono Oedjijono P.Hary Tjahja Palupi, Mustika Philipus Uli Basa Hutabarat Purnama Sukardi Purnama Sukardi Purnama Sukardi Purnama Sukardi Purwandaru Widyasunu R. Oktavia Rosita, Rita Eka Rusman, Ade Senny Helmiati Slamet Budi Prayitno Soedibya, Petrus Hary Tjahja Sofi Khoerun Nisa Sri Marnani Sri Marnani Sri Marnani Steinhagen, Dieter Syukri, Ningam Taufik Budhi Pramono Taufik Budhi Pramono Taufik Budhi Pramono Tika Widiastuti Tjahja, P.Hary Tohap Simangunsong Triyanto Triyanto Undiono Undiono Vaediyasari, Dahniar Wibowo, Satrio Haryu Widhiatmoko Herry Purnomo Wijaya, Rudy Yohanes Evander Yohanes Harvinda