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All Journal Jurnal Online Mahasiswa (JOM) Bidang Ilmu Sosial dan Ilmu Politik Jurnal Berita Sosial THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Teknik Kimia Jurnal Onoma: Pendidikan, Bahasa, dan Sastra JUMAN Tools (Jurnal Manajemen Tools) Journal of Language, Literature, and Teaching Jurnal Ilmiah Kesehatan Journal of Integrated Agribusiness Journal of Public Administration and Government (JPAG) Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Anakes: Jurnal Ilmiah Analis Kesehatan Window of Public Health Journal Masker Medika JIKEM: Jurnal Ilmu Komputer, Ekonomi dan Manajemen PROFESSIONAL HEALTH JOURNAL ARRUS Journal of Mathematics and Applied Science Jurnal Peternakan Lokal Bulletin of Science Education Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Jurnal Disastri: Pendidikan Bahasa dan Sastra Indonesia Bulletin of Pedagogical Research Jurnal Cakrawala Mandarin Journal of Muslim Community Health (JMCH) EKONOMIKA45 Jurnal Penelitian, Pendidikan, dan Pembelajaran Khidmah: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmiah Dan Karya Mahasiswa Jurnal Pengabdian Harapan Ibu (JPHI) Jurnal Bisnis dan Manajemen (JURBISMAN) Maeswara: Jurnal Riset Ilmu Manajemen dan Kewirausahaan Journal Health Applied Science And Technology (JHAST) International Journal of Education, Language, Literature, Arts, Culture, and Social Humanities Jurnal Bakti Dirgantara Jurnal Ilmiah Multidisiplin Keilmuan Mandira Cendikia (JIMK-MC) Blaze: Jurnal Bahasa dan Sastra dalam Pendidikan Linguistik dan Pengembangan IPSSJ Jurnal Silih Asih
Indah Sari
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Journal : Masker Medika

 1 
ANALISA METODE FIKSASI KERING MENGGUNAKAN GIEMSA DAN FIKSASI BASAH MENGGUNAKAN PAPANICOLAOU PADA PEMERIKSAAN PAP SMEAR Indah Sari; Bastian Darwin; Try Era Realita
Masker Medika Vol 9 No 1 (2021): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v9i1.456

Abstract

Kanker serviks adalah penyakit yang ditandai dengan pertumbuhan sel yang tidak terkontrol dan penyebaran sel abnormal yang terjadi di serviks. World Health Organization (WHO) menjelaskan bahwa kanker serviks merupakan penyebab kematian nomor dua pada perempuan dari seluruh penyakit kanker yang ada. Pap smear merupakan pemeriksaan yang dilakukan untuk mendeteksi kanker serviks. Pemeriksaan pap smear menggunakan bahan pemeriksaan swab vagina yang harus dilakukan fiksasi basah sebelum pewarnaan papanicolaou dan fiksasi kering sebelum pewarnaan giemsa. Penelitian bertujuan untuk menganalisa metode fiksasi basah menggunakan pewarnaan papanicolaou dan fiksasi kering menggunakan pewarnaan giemsa pada pemeriksaan pap smear yang dilakukan di Laboratorium Patologi Anatomi Dyatnitalis Palembang. Jenis penelitian menggunakan cross sectional dengan rancangan penelitian menggunakan intact group comparison. Sampel terdiri atas 8 responden masing-masing dilakukan fiksasi basah diwarnai dengan papanicoloau dan fiksasi kering diwarnai dengan giemsa. Penelitian dilakukan mulai dari persiapan pasien, pengambilan bahan pemeriksaan, pengolahan bahan pemeriksaan, analisis dan hasil pemeriksaan. Hasil uji Chi-Square diketahui bahwa nilai signifikan adalah p = 0,064. Nilai p yang didapatkan adalah pĖƒ0,05. Hasil penelitian dapat disimpulkan bahwa tidak terdapat perbedaan antara metode fiksasi kering menggunakan giemsa dan fiksasi basah menggunakan papanicolaou pada pemeriksaan pap smear.
ANALISA JUMLAH KOLONI BAKTERI Escherichia coli PADA MEDIA NUTRIENT AGAR DENGAN PELARUT AKUADES DAN AIR MINUM DALAM KEMASAN (AMDK) Bastian Darwin; Indah Sari; Annisa Fitri
Masker Medika Vol 9 No 1 (2021): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v9i1.457

Abstract

Pendahuluan: Penyakit diaere yang di sebabkan Escherichia coli patogen bahwa ada sekitar dua miliar kasus diare diseluruh dunia setiap tahun, dan 1,9 juta anak balita meninggal akibat diare setiap tahunnya. Standar pelarut dalam pembuatan media nutrient agar adalah akuades, Tetapi di daerah-daerah tertentu masih belum terjangkau untuk mendapatkan akuades, selain harganya yang mahal jangkauan untuk mendapatkan akuades tidaklah mudah karena alat untuk pembuatan akuades masih cukup mahal. upaya yang dapat dilakukan adalah menggunakan pelarut alternatif, dimana air minum dalam kemasan adalah air baku yang telah diproses, dikemas, dan aman diminum. Tujuan untuk mengetahui perbedaan jumlah koloni bakteri E.coli pada media nutrien agar dengan pelarut akuades dan air minum dalam kemasan. Metode Penelitian: Eksperimen laboratorium, yang dilakukan di Laboratorium Mikrobiologi Fakultas Sains dan Teknologi Institut Ilmu Kesehatan dan Teknologi Muhammadiyah Palembang. Sampel berupa Strain Murni Bakteri Escherichia coli ATCC 25922. Hasil: penelitian didapatkan jumlah koloni pada media nutrient agar dengan pelarut akuades mendapatkan nilai rata-rata 54 koloni, pada pelarut air minum dalam kemasan mendapatkan rata-rata 59 koloni. Dan uji-T berpasangan (paired sampel T test) di dapatkan nilai p =>0.112. Kesimpulan: Penelitian ini bahwa tidak terdapat perbedaan signifikan jumlah koloni bakteri E.coli pada media nutrient agar dengan pelarut akuades dan air minum dalam kemasan
PERBEDAAN KADAR FERITIN SERUM PADA REMAJA MENSTRUASI DAN TIDAK SEDANG MENSTRUASI indah sari
Masker Medika Vol 10 No 2 (2022): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v10i2.491

Abstract

Feritin adalah protein penyimpan zat besi utama yang ditemukan pada jaringan tubuh manusia. Fungsi feritin ialah sebagai penyimpanan zat besi terutama di dalam hati, limpa dan sumsum tulang. Kadar feritin serum merupakan indikator spesifik yang sering digunakan untuk menunjukkan ketersediaan besi tubuh karena protein berikatan dengan cadangan besi di dalam tubuh Pengeluaran darah selama menstruasi menunjukkan kehilangan simpanan zat besi secara cepat sesuai dengan banyaknya darah yang keluar sedangkan semakin lama wanita mengalami menstruasi maka semakin banyak pula darah yang keluar dan semakin banyak kehilangan timbunan zat besi. Oleh karena itu menstruasi merupakan golongan yang cenderung mengalami defisiensi besi. Penelitian ini bertujuan untuk mengetahui hasil kadar feritin serum pada remaja menstruasi dan tidak sedang menstruasi. Jenis penelitian ini adalah Cross Sectional Komparatif. Penelitian ini dilakukan di Institut Ilmu Kesehatan dan Teknologi Muhammadiyah Palembang dan di Balai Besar Laboratorium Kesehatan (BBLK) Palembang pada tanggal 08-17 Februari 2022 dengan jumlah sampel sebanyak 20 sampel. Hasil penelitian memiliki nilai rata-rata 22,88 ng/mL untuk remaja yang sedang menstruasi dan 22 ng/mL untuk remaja yang tidak sedang menstruasi. Hasil uji T berpasangan didapatkan nilai p < 0,05. Kesimpulan dari penelitian yaitu ada perbedaan hasil kadar feritin serum pada remaja menstruasi dan tidak sedang menstruasi.
PERBEDAAN KADAR HEMOGLOBIN SEBELUM DAN SESUDAH AKTIVITAS FISIK BERAT indah sari
Masker Medika Vol 11 No 1 (2023): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v11i1.516

Abstract

Introduction: Hemoglobin is an erythrocyte tetrameric protein that binds to a non-protein molecule, the Iron porphyrin compound called heme. The most important function of hemoglobin is transporting oxygen (O2) from the lungs to the peripheral tissues and transporting carbon dioxide (CO2) from the tissues to the lungs for exhalation to the outside. Factors that affect hemoglobin levels one of physical activity. Strenuous physical activity that releases a lot of sweat such as aerobics, cycling (19 ā€“ 22 km/h), swimming, tennis and running. The study aimed to determine the difference in hemoglobin levels before and after heavy physical activity in the form of running for 5 minutes with a heart rate frequency > 145 times/minute. Method: data analysis using Paired Sample T-Test SPSS. This research was conducted at the Muhammadiyah Institute of Health Sciences and Technology Palembang in March 2021 with a total of 32 respondent. Results: the average hemoglobin levels before physical activity is 15,1 g/dL while hemoglobin levels after heavy physical activity is 15,3 g/dL, based on the Test Paired Sample t-Test get p > 0.05 that is no difference between the two. Discussion: there was no difference between hemoglobin levels before and after heavy physical activity.
PENGARUH VOLUME SAMPEL DARAH DENGAN ANTIKOAGULAN K2EDTA TERHADAP KADAR HEMATOKRIT indah sari
Masker Medika Vol 11 No 2 (2023): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v11i2.551

Abstract

Introduction: Pre-analytical stage errors contributed the most to laboratory errors (46-77.1%). Some things that include pre-analytical errors include hemolysis (53.2%), insufficient specimen volume (7.5%), unreadable handwriting (7.2%), wrong specimen, specimen has clot, vacuum error container or type of anticoagulant, specimen volume ratio and inappropriate anticoagulant. Research: aims to determine the effect of blood sample volume with K2EDTA anticoagulant on Hematocrit Levels. Methods: manual method and automatic method. Data analysis used normality test, data transformation test, Wilcoxon test using SPSS. The sampling technique used Nonprobability in the form of Purprosive sampling. This research was conducted at the Muhammadiyah IKesT Hematology Laboratory and RS. Bunda Palembang in February ā€“ March 2023. The study used EDTA blood samples from grade 2 and 3 female students DIV IKesT Muhammadiyah Palembang Medical Laboratory. Results: the average hematocrit examination with 3CC EDTA blood volume was 54.1% and 1CC EDTA blood was 42.3%. The conclusion of the study is that there is an effect of blood sample volume with K2EDTA anticoagulant on hematocrit levels. Discussion: there is an effect of blood sample volume with K2EDTA anticoagulant on hematocrit levels.
STABILITAS POOLED SERA BERDASARKAN LAMA DAN SUHU PENYIMPANAN TERHADAP MUTU PEMERIKSAAN PROTEIN TOTAL indah sari
Masker Medika Vol 12 No 1 (2024): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v12i1.613

Abstract

Introduction: internal quality stabilization in clinical chemistry laboratory can be carried out by conducting serum control examination which aims to test or assess the validity of laboratory examination results and the results issued by the laboratory in accordance with the criteria of the examination results. The use of pooled sera made from the remaining patient specimens can be an alternative to reduce the cost of quality control of laboratory examinations. Total protein levels in the blood can be determined by examination in a clinical laboratory as an indicator of protein status in the body. A common problem in clinical laboratories is maintaining the stability of serum analytes during sample storage. Purpose of the study: to determine the stability of pooled sera based on the duration and storage temperature of the quality of the total protein examination. Method: the type of research is experimental, with the design of the study is time series control group design. The sample in this study was a control serum in the form of pooled sera stored in a freezer that was examined for total protein every day for 4 weeks. Results: mean total protein test was 6.1 mg/dl in commercial control and 6.6 mg / dl in pooled sera. Discussion: there is an effect of long storage in the freezer on the stability of total protein examination.
PEMANFAATAN MINYAK WORTEL YANG RAMAH LINGKUNGAN SEBAGAI PENGGANTI XYLOL DALAM PROSES DEPARAFINISASI indah sari
Masker Medika Vol 12 No 2 (2024): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v12i2.682

Abstract

Introduction: hematoxylin Eosin is a common dye used in histology laboratory examination. One of the stages of hematoxylin staining of eosin is the process of deparafinization. Deparafinisasi is a process to remove or dissolve paraffin from tissue and perform a clearing process before being observed under a microscope so that maximum color absorption at the time of tissue staining and look clear. The deparafinisai process is usually done by using xylol to dissolve paraffin in the form of fat. Xylol is commonly used as an ingredient for clearing and deparafinization processes. Continuous use of xylol can cause toxicity to the body and health. Medical laboratory workers, especially histology officers in charge of tissue coloring have a dangerous risk of chemical, biological, mechanical and environmental hazards so that a safer and cheaper alternative to xylol is needed. Alternative use of xylol is done to minimize harm to health and the environment, among others, by finding a substitute for xylol. Some natural ingredients such as carrot oil, pine oil, rose oil and olive oil can be used instead of xylol. Carrots relatively do not contain sulfur elements, so they do not cause air pollution, and can also improve the efficiency of agricultural resource utilization. Purpose of the study: to utilize environmentally friendly carrot oil as a substitute for xylol in the deparafinization process. Method: the research design used is true experiment design. Samples of human breast tissue (mammae) to see the results of the examination using an alternative reagent carrot oil and xylol as a control. The examination data will be processed by descriptive analysis based on the results of the assessment score of tissue preparation. Result: hematoxylin eosin staining using xylol get very good results of 100% while using carrot oil get very good results of 87.5% in the process of deparafinization. Discussion: Carrot oil can be used as a substitute for xylol in the process of deparafinization.
Co-Authors Abdul Rahman Achmad Hidir Adho Alif Akbar Aisah Oktavia Andi Asrina Anindia Rizkila Cahyani Anis Safitri Annisa Fitri, Annisa Ardison Asri Ari Purnomo Aristoteles Arya Budi Pratama Asfarony Hendra Nazwin Bastian Bastian Bastian Bastian Betti Dian Wahyuni Buyung Surahman Choiriyah Choirunnisak Citra Ayu Anggraini Della Ardila Denies Priantinah Despy Pallu Devita Rahmawati Putri Diding Rahmat Dini Thiyana Luthfi Eddy Jajang Jaya Atmadja Era Rahayu Erni Suryati Fakhrina Fakhrina Faranissa Dzulliya Syafitri Fatmawati, Fatmawati Ghufron Faqih Hafifah, Ifa Hamriani Hasby Pri Choiruna Hendy Kurniawan Heni Maria Hidayatunnikmah, Nina Hilda Hilda Idris, Fairus Prihatin Isti Harkomah Jenuri Khairiyah Putri Kokom Siti Komariah Lasmauli Niverita Luthfi Hayatun Maharani Maria Ayu Andira Marwiyah Monna Satria Yunita Monna SatriaYunita Mudinillah, Adam MUHAMMAD AMIR Muhammad Syahril Muntoro Muntoro Mustamin H. Idris Niru Anita Sinaga Niza Ayuningtias Nofrida Saswati Nur Ulmy Mahmud Nurafni Oktafia Siringo-ringo Nurhidayanti Nurti Budiyanti NURUL HIDAYAT Nyna Puspita Ningrum Pina Lestari Raharjo, Resdianto Permata Rahmayanti Rahmayanti Reni Junika Familianti Resti Komala Sari Reza Apriani Riska Asmidar Robin Chandra Rosidah Selpiana Siregar, Zeta Claudia Sandra Siti Munawaroh Siti Zahara Sitti Sabiyya SRI RAHAYU Subandi Susi Nawanti Syahriana Sabil Tiara Adelia Putri Try Era Realita Winda Kustiawan Yoserizal " Yuli Kisilowati Yusriani, Yusriani