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Unsunnidhal, Lalu
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Deteksi Salmonella typhi Berbasis Metode PCR pada Terasi Kering Padat Blok Produksi UMKM di Kabupaten Lombok Timur Ariyana, Mutia Devi; Unsunnidhal, Lalu; Perdhana, Firman Fajar; Febriandito, Muhammad ‘Aidil; Handayani, Baiq Rien; Zuhdia, Lulu Diani
Pro Food Vol. 11 No. 2 (2025): Pro Food (Jurnal Ilmu dan Teknologi Pangan)
Publisher : Fakultas Teknologi Pangan dan Agroindustri, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/profood.v11i2.508

Abstract

Shrimp paste is a fermented shrimp product that is at risk of being contaminated by pathogenic microorganisms, thus potentially causing foodborne diseases. The method for detecting pathogenic microorganisms that has proven to have high specificity and sensitivity in directly detecting the presence of pathogenic bacteria in food samples is the DNA detection method using Polymerase Chain Reaction (PCR). This research aims to detect the presence of pathogenic bacteria, specifically Salmonella typhi, in shrimp paste products produced by several Small and Medium Enterprises (SMEs) in East Lombok Regency using the PCR method. The research samples used were solid block dried shrimp paste from three SMEs in East Lombok Regency. The research results show that the DNA of Salmonella typhi bacteria was not detected in all tested samples. These results indicate that the shrimp paste from the three SMEs in East Lombok has met one of the microbiological quality requirements for shrimp paste based on SNI 2716:2016, which is the absence of Salmonella contamination.
Screening, Characterization, and Cultivation of Cellulase-Producing Bacteria as Probiotic Candidates for Poultry Anwar, Khairil; Sukarne; Unsunnidhal, Lalu; Fitriyan Fadjar Suryadi, Muh. Aidil; Junia Ayu Ninggrum, ⁠Vidia; Aryadin Putra, Ryan; Al Gifari, Zaid; Septian, I Gede Nano; Aminurrahman; Ali, Muhamad
Jurnal Sain Peternakan Indonesia Vol 20 No 4 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.4.179-190

Abstract

Probiotics play an essential role in regulating gut microbiota and increasing feed digestibility in poultry. This study aimed to screen and characterize cellulase-producing bacteria as poultry probiotic candidates. Among the five isolates tested, isolates I5 and BP had cellulase activity, as indicated by clear zones surrounding the colonies on carboxymethyl cellulose (CMC) agar plates. Isolate I5 was more tolerant to low pH and 0.3% bile salts than isolate BP, indicating probiotic potential. Isolate I5 was selected for cultivating in tempeh wastewater-molasses medium (TM) and LB medium at 37 °C with shaking at 120 rpm. The results demonstrated that bacterial growth in TM medium was significantly lower  (p < 0.001) than in LB medium. Importantly, bacterial growth in TM medium reached an optical density (OD₆₀₀) of 0.415 after 6 hours of incubation, indicating its adaptability to tempeh wastewater-molasses medium. These findings suggest that the TM medium promotes bacterial growth and proliferation, supports probiotic and enzyme production for use in poultry feed supplementation, and reduces cultivation costs. Therefore, the use of agro-industrial waste provides a cost-effective alternative for cultivating cellulase-producing probiotics, thereby contributing to value-added waste management, sustainable poultry production, and circular bio-economy practices.
Petroleum Degradation by Bacteria Explored from Logending Mangrove Sediments Apriliani, Herlina; Pramono, Hendro; Oedjijono, Oedjijono; Widodo, Wimbuh Tri; Kristianto, Sonny; Putri, Rury Eryna; Unsunnidhal, Lalu
Biota Vol 12 No 1 (2026): Jurnal Biota 2026
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i1.29245

Abstract

Oil spills resulting from shipping activities, tanker-based oil transportation, and fuel oil usage can cause coastal pollution, particularly in sensitive ecosystems such as mangroves. More than 90% of petroleum consists of hydrocarbons with complex carbon chain structures, making them difficult to decompose. Biological remediation using microorganisms offers a promising alternative for pollution mitigation, as microbes can degrade petroleum components and oxidizing hydrocarbons. This study aimed to evaluate the petroleum-degrading ability of selected bacterial isolates obtained from mangrove sediments at Logending Beach. The research employed experimental and survey methods. The primary parameter measured was Total Petroleum Hydrocarbons (TPH), while supporting parameters included pH and bacterial population density. The study consisted of several stages, including bacterial isolation, screening, and evaluation of the petroleum degradation capacity of selected isolates. The results identified two potential bacterial isolates capable of degrading crude oil. Isolate LG62 exhibited a degradation efficiency of 71.40%, while isolate LG105 showed a degradation efficiency of 57.10%. Petroleum concentrations of 2% (v/v) and 5% (v/v) were degraded more effectively than higher concentrations. Overall, the two bacterial isolates (LG62 and LG105) from Logending mangrove sediments demonstrated significant potential as bioremediation agents for petroleum hydrocarbon contamination.
Co-Authors Ahmad Alamsyah Al Gifari, Zaid Amaliah, Wenny Amaliyah, Wenny Amaro, Moegiratul Aminurrahman Anggraini, Ines Marisya Dwi Antesty, Sella Apriliani, Herlina Asmarani Kusumawati Baiq Rien Handayani, Baiq Rien Cicilia, Siska Dody Handito, Dody Dwi Prihantoko, Kurniawan Eko Basuki, Eko Febriandito, Muhammad ‘Aidil Fihiruddin, Fihiruddin Firman Fajar Perdhana Fitriyan Fadjar Suryadi, Muh. Aidil Hasma Hasma Hendro Pramono Ine Karni Jannah, Nuzuliya Miftahul Junia Ayu Ninggrum, ⁠Vidia Khairil Anwar Kristianto, Sonny Made Sriasih, Made Miraj Fuadi Muh. Aidil Fitriyan Fadjar Suryadi Muhamad Ali Mutia Devi Ariyana, Mutia Devi Nurul Inayati Oedjijono Oedjijono, Oedjijono OKI SAPUTRA Pertiwi, Made Gendis Putri Putra, Ryan Aryadin Putri, Sasi Fhatmayani Qabul Dinanta Utama Rasyda, Riezka Zuhriatika raudatul jannah Rezki Amalyadi Rina Andriati, Rina Rosyidi, Muhammad Subhan Bahruddin Rury Eryna Putri S., Ince Siti Wardatullatifah Septian, I Gede Nano Setyaning Pawestri Sri Ernawati Sri Widyastuti Sukarne Sukarne Sukarne, Sukarne Suryadi, Muh. Aodil Fitriyan Fadjar Wardatullatifah , Ince Siti Wardatullatifah S., Ince Siti Wimbuh Tri Widodo Yasa, I Wayan Sweca Yesica Marcelina Romauli Sinaga Zaid Al Gifari Zuhdia, Lulu Diani