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Unsunnidhal, Lalu
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Nursing Public Health Veterinary Other

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Screening, Characterization, and Cultivation of Cellulase-Producing Bacteria as Probiotic Candidates for Poultry Anwar, Khairil; Sukarne; Unsunnidhal, Lalu; Fitriyan Fadjar Suryadi, Muh. Aidil; Junia Ayu Ninggrum, ⁠Vidia; Aryadin Putra, Ryan; Al Gifari, Zaid; Septian, I Gede Nano; Aminurrahman; Ali, Muhamad
Jurnal Sain Peternakan Indonesia Vol 20 No 4 (2025)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.20.4.179-190

Abstract

Probiotics play an essential role in regulating gut microbiota and increasing feed digestibility in poultry. This study aimed to screen and characterize cellulase-producing bacteria as poultry probiotic candidates. Among the five isolates tested, isolates I5 and BP had cellulase activity, as indicated by clear zones surrounding the colonies on carboxymethyl cellulose (CMC) agar plates. Isolate I5 was more tolerant to low pH and 0.3% bile salts than isolate BP, indicating probiotic potential. Isolate I5 was selected for cultivating in tempeh wastewater-molasses medium (TM) and LB medium at 37 °C with shaking at 120 rpm. The results demonstrated that bacterial growth in TM medium was significantly lower  (p < 0.001) than in LB medium. Importantly, bacterial growth in TM medium reached an optical density (OD₆₀₀) of 0.415 after 6 hours of incubation, indicating its adaptability to tempeh wastewater-molasses medium. These findings suggest that the TM medium promotes bacterial growth and proliferation, supports probiotic and enzyme production for use in poultry feed supplementation, and reduces cultivation costs. Therefore, the use of agro-industrial waste provides a cost-effective alternative for cultivating cellulase-producing probiotics, thereby contributing to value-added waste management, sustainable poultry production, and circular bio-economy practices.
In Silico and Toxicological Evaluation of Crescentia Cujete Aqueous Extract: Potential Anticancer Activity Against Breast Cancer Martha, Rahma Diyan; Fatimah, Fatimah; Wibowo, Syahputra; Kusuma Wardhani, Bantari Wisynu; Siregar, Josephine Elizabeth; Novita Coutrier, Elisabeth Farah; Fitrianita, Alfia; Pramono, Andri Pramesyanti; Nainggolan, Ita Margaretha; Nugraha, Yudhi; Danar, Danar; Abiyyu, Naufal; Unsunnidhal, Lalu; Khairullah, Aswin Rafif; Ansori, Arif Nur Muhammad; Parbuntari, Hesty; Widodo, Wimbuh Tri
Makara Journal of Science Vol. 30, No. 2
Publisher : UI Scholars Hub

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Abstract

Majapahit plant (Crescentia cujete), commonly found in Indonesia, has not been extensively explored for its medicinal properties. Given that many cancer treatments are derived from plants, this study aimed to examine the effects of plants on cancer chemical composition of the C. cujete fruit pulp extract, identify its primary compounds, and assess its potential as an antioxidant, anticancer agent, particularly for breast cancer. An insilico approach was used to evaluate the toxicity and interactions, of the compounds with cancer biomarker proteins, in addition to an in vivo Brine Shrimp Lethality Test (BSLT) for BSLT, toxicity screening using six concentrations of the extract (100, 200, 300, 400, and 600 ppm). The extract was obtained by boiling the fruit pulp in distilled water. Compound analysis using liquid chromatography-mass spectrometry (LC-MS) identified approximately 110 compounds, with kaempferol-3-O-rhamnoside (3.29%) and hesperidin (3.19%) being the most abundant. Toxicity analysis using ProTox 3.0 revealed that kaempferol-3-O-rhamnoside exhibited toxicity to the kidney, heart, and lungs, with probabilities of 0.68, 0.82, and 0.51, respectively. Hesperidin was toxic to the kidney and lungs, with probabilities of 0.76 and 0.66. Molecular docking studies with the X-ray spectrometer BRCA1 (P38398 - BRCA1_HUMAN) and BRCA2 (P51587–BRCA2_HUMAN) proteins showed that kaempferol-3- O-rhamnoside formed stable interactions with both proteins, with binding affinities of −7.0 kcal/mol for BRCA1 and -8.1 kcal/mol for BRCA2. Similarly, hesperidin demonstrated stable binding interactions with BRCA1 (binding affinity) of −7.7 kcal/mol) and BRCA2 (binding affinity of −9.0 kcal/mol). The LC50 value for the C. cujete fruit pulp extract was calculated as follows: determined to be 546 ppm (95% CI: 498.3–592.1 ppm), indicating that the extract is toxic and holds potential as an anticancer agent. While individual compounds exhibited organ-specific toxicity, the overall extract was nontoxic, suggesting its potential for further anticancer research. Future studies should focus on additional toxicity testing using cell cultures and mammalian models.
Co-Authors ABIYYU, NAUFAL Adi Sofyan Ansori, Muhammad Ahmad Alamsyah Al Gifari, Zaid Amaliah, Wenny Amaliyah, Wenny Amaro, Moegiratul Aminurrahman Andri Pramesyanti Pramono Anggraini, Ines Marisya Dwi Antesty, Sella Asmarani Kusumawati Baiq Rien Handayani, Baiq Rien Cicilia, Siska Danar, Danar Dody Handito, Dody Dwi Prihantoko, Kurniawan Eko Basuki, Eko fatimah Fatimah Febriandito, Muhammad ‘Aidil Fihiruddin, Fihiruddin Firman Fajar Perdhana Fitrianita, Alfia Fitriyan Fadjar Suryadi, Muh. Aidil Hasma Hasma Jannah, Nuzuliya Miftahul Junia Ayu Ninggrum, ⁠Vidia Khairil Anwar Khairullah, Aswin Rafif Kusuma Wardhani, Bantari Wisynu MARTHA, RAHMA DIYAN Miraj Fuadi Muh. Aidil Fitriyan Fadjar Suryadi Muhamad Ali Mutia Devi Ariyana, Mutia Devi Nainggolan, Ita Margaretha Novita Coutrier, Elisabeth Farah Nugraha, Yudhi Nurul Inayati OKI SAPUTRA Parbuntari, Hesty Pertiwi, Made Gendis Putri Putra, Ryan Aryadin Putri, Sasi Fhatmayani Qabul Dinanta Utama Rasyda, Riezka Zuhriatika raudatul jannah S., Ince Siti Wardatullatifah Septian, I Gede Nano Setyaning Pawestri Siregar, Josephine Elizabeth Sri Ernawati Sri Widyastuti Sukarne Sukarne Sukarne, Sukarne Wardatullatifah , Ince Siti Wardatullatifah S., Ince Siti Wibowo, Syahputra Wimbuh Tri Widodo Yasa, I Wayan Sweca Yesica Marcelina Romauli Sinaga Zuhdia, Lulu Diani