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Afiff , Usamah
Divisi Mikrobiologi Medik, Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Library & Information Science Medicine & Pharmacology Public Health Veterinary

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Journal : Current Biomedicine

 1 
Resistansi Escherichia coli patogen asal ayam petelur terhadap beberapa antibiotik Suhendi, Adnan Rizal; Afiff, Usamah; Handharyani, Ekowati
Current Biomedicine Vol. 2 No. 1 (2024): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.1.45-54

Abstract

Latar Belakang: Penggunaan antibiotik yang tidak tepat pada ternak menyebabkan munculnya resistansi bakteri terhadap antibiotik. Salah satu bakteri yang telah dilaporkan resistan adalah Escherichia coli sehingga pengobatan untuk infeksi bakteri ini menjadi kurang efektif. Tujuan: Penelitian ini bertujuan untuk mendeskripsikan resistansi E. coli patogen yang berasal dari usap kloaka ayam petelur terhadap antibiotik. Metode: Sebanyak 20 sampel usap kloaka diidentifikasi keberadaan E. coli melalui pengamatan makroskopis, mikroskopis, uji biokimia, dan fermentasi karbohidrat uji patogenisitas dilakukan dengan menggunakan media Congo red. Isolat yang patogen selanjutnya diuji kepekaannya terhadap 6 jenis antibiotik dengan uji kepekaan difusi cakram Kirby-Bauer. Hasil: Penelitian memperoleh 15 isolat E. coli dan uji Congo red mendapatkan 4 isolat E. coli patogen. Hasil penelitian menunjukkan bahwa 75% isolat resistan terhadap azitromisin, 100% isolat resistan intermediet terhadap doksisiklin dan siprofloksasin. Sementara itu, 75% isolat masih sensitif terhadap gentamisin dan 100% isolat sensitif terhadap kloramfenikol dan amoksisilin. Kesimpulan: Penelitian ini berhasil mengisolasi E. coli yang bersifat patogen dari ayam petelur yang telah resistan terhadap beberapa antibiotik yang diujikan.
Quantification and identification of bacterial presence in salted eggs Vindriati, Zukhrufa Vista; Afiff, Usamah; Purnawarman, Trioso
Current Biomedicine Vol. 3 No. 1 (2025): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.1.43

Abstract

Objective This study aimed to quantify the total bacterial count in salted eggs and identify bacterial species that may affect their quality. Methods Fifty samples consisted of 10 fresh salted eggs that passed the candling test, 10 portions of pasta dough at 0, 3, and 6 hours, and 10 fresh salted eggs that had failed the candling test (black egg yolk). Each sample was tested in triplicate. The total bacterial count was determined using the plate count agar method, and bacterial identification was based on phenotypic analysis, which included Gram staining and biochemical tests. Results The total bacterial load in fresh salted eggs was below the maximum limit set by SNI 7388:2009 (1×105 CFU/g), whereas the pasta dough and black egg yolk exceeded this limit. The identified bacteria included Escherichia spp., Enterobacter spp., Proteus spp., Staphylococcus spp., Pseudomonas spp., and Bacillus spp. Conclusion Although the bacterial count in salted eggs meets SNI standards, the presence of potentially harmful bacteria highlights the need for enhanced hygiene and sanitation measures to be implemented during the production of salt eggs.
Co-Authors Abdul Samik Abdul Zahid Agus Wijaya Agus Wijaya Agustin Indrawati Alla Asmara Anggia Murni Wijiati Annisa Madyanti Geminastiti Parampasi Anriansyah Renggaman Ardilasunu Wicaksono Arini Resti Fauzi Asah Hilaliah Astuti, Yohana Tri Aulia Andi Mustika Beginer Subhan Chairani Ridha Maghfira Darna Andrian Ramadhan Dea Indriani Astuti Dedi Rahmat Setiadi Deni Noviana Denny Widaya Lukman Denny Widya Hikman Destri Prameswari, Alvira Dinamella Wahjuningrum Edi Sukmawinata Edi Sukmawinata Ekowati Handharyani Etih Sudarnika Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Faoziyatunnisa, Nurul Fauzi, Arini Resti Gustilatov, Muhamad Hardianti, Aprilia Hary Krettiawan Islami, Nurfara Ismahyuningsih, Revita Julie Ekasari Karen Lee koekoeh santoso Leng , Ang Jia Maria Luisa MNB Klobongona Men, Lydia Pow Kar Merlia Andriyani Mia Setiawati Michella Hoseana Wijaya MUHAMMAD AGIL Muhammad Arif Mulya MUNTI YUHANA Nabila Swarna Puspa Hermana Nauval Firdana, Chorrysa Nor Jannah, Nor Nugraha, Arifin Budiman Nuri Kamaliah, Syarifah Nursa Rima Putri Purnamaningrum, Nora Dyah Ayu Rahmat Hidayat Ridi Arif Risa Tiuria Risqika Akla Velayati Rizal Dwinto Rochman Ronald Tarigan Rotinsulu, Dordia Anindita Safika S, Safika Septiriyanti, Diyah sri murtini . Sri Nuryati Srihadi Agungpriyono Sudrajat, R Herman Suhendi, Adnan Rizal Sukenda . Suleman, Gabriella Augustine Supratikno, Supratikno Surya Agus Prihatno Tambun, Andreas Titiek Sunartatie Trioso Purnawarman Tsani Untsa, Agista Ulandari, Rafika Ulfatin Khoiriyah Herowati Vindriati, Zukhrufa Vista Wicaksono, Baref Agung WIDANARNI WIDANARNI Xin, Leaw Yi Ying, Megan Chan Zhi Yosua Kristian Adi Yusuf Ridwan