Article Per Year (5 Year)
p-Index From 2021 - 2026
3.366
P-Index
This Author published in this journals
All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrointek Jurnal Penelitian Pascapanen Pertanian agriTECH Biota Warta IHP (Warta Industri Hasil Pertanian) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Forest and Society Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL PANGAN Jurnal Mutu Pangan : Indonesian Journal of Food Quality Budimas : Jurnal Pengabdian Masyarakat Jurnal Fakultas Teknik UNISA Kuningan Livestock and Animal Research Journal of Livestock and Animal Health (JLAH) Food and Agro-industry (FAGI) Journal Food and Agro-industry (FAGI) Journal Jurnal Standardisasi Jurnal Penginderaan Jauh dan Pengolahan Data Citra Digital
Ratih Dewanti -Hariyadi
Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB, Darmaga, Jl. Lingkar Akademik, Babakan, Kec. Dramaga, Bogor, Jawa Barat 16680
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

Published : 48 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 48 Documents
Search

 1   2   3   4   5 
Ketahanan dan Kulturabilitas Cronobacter Sakazakii terhadap Stres Kering pada Simulasi Proses Pengeringan Siti Nurjanah; Ratna Nurmalita Sari; Ratih Dewanti-Hariyadi
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 4 No. 2 (2017)
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cronobacter sakazakii is an opportunistic pathogen that has been isolated from several dried food products and reported can survive in dry conditions. Survival on dry stress can be observed using microscopy due to losses of their culturability in the medium. This study aimed to observe the survival and culturability of C. sakazakii on dry stress and ability to recover their culturability or resuscitation. This research was carried out in 3 steps: drying cells by desiccation, survival cell enumeration, and resuscitation. The bacteria used were two isolates Green fluorescence protein (pGFPuv) mutants of C. sakazakii, namely E2 and Yrt2a, that have ampicillin resistance and similar growth pattern with their wild-type. Desiccation was conducted by placed bacterial cells in incubator at 30, 35, 400 and 500C for 2 hours and air-drying stored at 210C for 72 hours. The culture able cells were enumerated on tryptone soy agar (TSA) and total cells include non-culturable cells were enumerated using fluorescence microscopy. Both of C. sakazakii isolates can survive for all of treatment. Total cells of E2 and Yrt2a decreased 2-3 and 4-5 log cells respectively. Both of isolates loss their ability to grow on medium, however suplementation the medium growth using pyruvate can resuscitate their cells and recover their culturability. Resuscitation of C. sakazakii cells might be as potential risk for increasing bacterial contaminant in food.
Inaktivasi Bakteri Patogen Planktonik dan Biofilm oleh Sanitaiser Komersial Ratih Dewanti Hariyadi; Cynthia CynthiaCynthia
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 1 No. 2 (2014): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cleaning and sanitizing are important aspects in providing quality and safe foods. In the US, contaminated equipment is the third most important factor contributing to foodborne disease outbreaks. The objective of this study was to evaluate the effectiveness of commercial sanitizers against planctonic cells and biofilms of pathogenic bacteria Salmonella Typhimurium and Staphylococcus aureus. Commercial sanitizers used in this study were single sanitizers (quaternary ammonium, peroxy acetic acid, coconut fatty acid) and combined sanitizers (Oxyquart®, Bioclean 520®, Bioclean 540®) at the recommended application concentrations. Of the sanitizers tested on planctonic cells, peroxy acetic acid at 100 ppm as well as Oxyquart® and Bioclean 540® at concentrations of 0.010% are effective against S. Typhimurium; while Oxyquart® at a concentration of 0.005% is the most effective sanitizer against S. aureus. The study also shows that Oxyquart® is more effective against biofilms and capable of inactivating 3.94-5.02 log CFU/cm2 of S. Typhimurium or S. aureus biofilm. 
Kajian Penerapan Peraturan Menteri Pertanian Nomor 53 Tahun 2012 untuk Pengendalian Aflatoksin pada Pala Ni Made Vina Citanirmala; Winiati Pudji Rahayu; Ratih Dewanti Hariyadi
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 3 No. 1 (2016): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Nutmeg as Indonesia main exported commodity frequently contaminated by aflatoxin and often rejected by European Union. To improve safety of nutmeg, Ministry of Agricultural (MA) issued a regu- lation number 53 in 2012 regarding good practices on nutmeg. The objectives of this research were to (1) evaluate implementation of the MA regulation at nutmeg supply chain in Siau Barat District (2) identify critical points for mold growth and/or aflatoxin formation along the nutmeg supply chain. This research conducted through (1) surveys to 60 farmers, 10 middlemen, 2 exporters and 3 government officials as respondents; (2) identification of critical steps using HACCP decision trees. The evaluation of MA regu- lation showed that at farmer level was poor for postharvest handling (54.4%) caused by lack of facilities and infrastructure (57.2%), although for harvest was moderate (70.4%). At the middleman level, handling practices at postharvest was moderate (62.5%), although not supported with facilities and infrastructure (36.7%). Handling practices at exporter level was generally good, however supervision at government official level was poor (56.3%). Critical control points determined at farmer level were harvesting and drying, at middlemen level were receiving, drying and storage, while at exporter level were receiving and shipment. 
Pengembangan Sistem Cara Produksi Makanan Enteral yang Baik (CPMEB) dan Aplikasinya di Rumah Sakit Amiroh Amiroh; Winiati Pudji Rahayu; Ratih Dewanti Hariyadi
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 3 No. 1 (2016): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hospital formula enteral food is a special food targeted specifically for a group of people with health risk. Therefore its safety needs to be controlled more stringently for example through the application Good Manufacturing Practices (GMP). Presently, the guidelines for good processing practices for enteral food is not available yet. This research aims to develop a Good Enteral Food Manufacturing Practices (GEFM) consisting of a guideline as well as a sysstematic audit system that can be applied easily in the hospital. The system was developed based on the Ministry of Health of Republic of Indonesia Regulation Number:1096/MenKes/PER/VI/2011 on hygiene and sanitation for food service industries and other relevant references. Based on the literature review and trials, thirteen aspects were defined for the require- ments; including four main aspects pertaining production room, production equipment, process control and workers' hygiene. Other aspects include building and its facilities, sanitation facility, raw materials storage, monitoring, pest control, distribution, training, patient feeding, and documentation. An audit trial at a hospital Y suggested that its enteral food production unit was categorized as good; but needed improve- ment in several aspects such as: production equipment storage, hygiene and sanitation maintenance, food distribution and food handle training. 
Kajian Kesesuaian Standar Cemaran Kimia (Logam Berat dan PAH) pada Produk Perikanan di Indonesia dengan Standar Negara Lain dan Codex Oryssa Sathalica Pradianti; Winiati Pudji Rahayu; Ratih Dewanti- Hariyadi
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): Juni 2019
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v14i1.560

Abstract

AbstrakPangan dapat terkontaminasi oleh cemaran kimia karena penanganan dan pengolahan pangan yang tidak sesuai. Tujuan dari penelitian ini adalah 1) mengidentifikasi mayoritas penyebab penolakan produk perikanan Indonesia, 2) menelaah standar cemaran kimia pada produk perikanan, khususnya logam berat yang ada di Indonesia, Codex  Alimentariurs Commision (CAC) dan negara-negara lain, serta 3) memberikan rekomendasi bagi pemerintah selaku regulator dalam proses perumusan suatu standar. Dokumen standar cemaran kimia pada produk perikanan dikumpulkan dari dokumen/peraturan yang dikeluarkan oleh Badan Pengawasan Obat dan Makanan (BPOM), Badan Standardisasi Nasional (BSN), CAC, dan 11 negara lain yaitu Uni Eropa, Kanada, China, Korea Selatan, Vietnam, Amerika Serikat, Jepang, Malaysia, Singapura, Thailand, dan Australia. Hasil penelitian menunjukkan bahwa dari 164 notifikasi penolakan produk perikanan Indonesia di Uni Eropa, Kanada, dan Korea Selatan selama 10 tahun (2008-2017), penolakan produk perikanan tertinggi disebabkan oleh adanya cemaran kimia merkuri dan metilmerkuri pada ikan todak sebesar 27%, kadmium pada gurita sebesar 5% dan benzo[a]piren pada ikan asap sebesar 3%. Batas maksimum cemaran kimia untuk arsen, kadmium, dan timbal (pada ikan predator) di Indonesia yang terdapat pada Peraturan Kepala (Perka) BPOM Nomor 5 Tahun 2018 lebih rendah dibandingkan dengan yang terdapat di SNI 7387:2009 maupun yang ditetapkan oleh CAC serta negara lain. Indonesia telah menetapkan batas maksimum benzo[a]piren pada ikan asap, sementara itu CAC hanya menetapkan code of practice terhadap benzo[a]piren. Peraturan cemaran logam berat belum sepenuhnya dipedomani oleh para eksportir sehingga masih terdapat penolakan produk perikanan Indonesia. Hal ini menunjukkan masih perlu dilakukan pengawasan terkait kandungan logam berat yang terdapat pada produk perikanan di Indonesia. Code of practice terkait proses pengolahan pangan direkomendasikan untuk diterbitkan guna meminimalisir kandungan benzo[a]piren. Compliance Assessment of Chemical Contaminant Standard (Heavy Metal and PAH) for Fishery Products in Indonesia with those of Other Countries and CodexAbstractFood can be contaminated by chemical contamination through inappropriate food handling and processing. The purpose of this study aims to: 1) identify the chemical contamination caused majority of the rejection of Indonesia fishery products, 2) reviewing the chemical standards of contamination fishery products in Indonesia, Codex Alimentariurs Commision (CAC) and other countries, and 3) provide recommendations for the setting of chemical contamination standards in fishery products. Chemical contamination standards were collected from regulations issued by the Indonesia National Agency of Drug and Food Control (NADFC), National Standardization Agency of Indonesia, CAC, and 11 other countries: European Union/EU, Canada, China, South Korea, Vietnam, United States of America, Japan, Malaysia, Singapore, Thailand, and Australia. The results showed that in the 10 years period (2008-2017), there were 164 rejection notifications in EU, Canada, and South Korea and the highest rejection was caused by chemical contamination i.e. 27% caused by mercury and methylmercury in swordfish, 5% caused by cadmium in octopus and 3% caused by benzo[a]pyrene in smoked fish. The maximum limit of chemical contamination for arsenic, cadmium, and lead (predatory fish) in Indonesia as stated in the Regulation of the Head of NADFC Number 5 of 2018 lower than the maximum limit set in SNI 7387: 2009 and sets in CAC and other countries. Indonesia set the maximum limit for benzo[a]pyrene in smoked fish, whereas CAC published the code of practice to avoid a benzo[a]pyrene formation. Regulations of maximum limit for heavy metal contamination have not been fully guided by fisheries exporters, so that there was still rejection of Indonesian fishery products. The action needed is tightening the monitoring of heavy metal in fishery products in Indonesia. Code of practice to avoid benzo[a]pyrene formation is recommended to set up to minimize the benzo[a]pyrene formation on food processing.    
Prevalensi Gen tdh dan trh Vibrio parahaemolyticus pada Udang Vaname Di Wilayah Indramayu, Jawa Barat Yusma Yennie; Ratih Dewanti Hariyadi; Achmad Poernomo
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): Juni 2015
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v10i1.245

Abstract

Penelitian ini bertujuan untuk mengetahui prevalensi Vibrio parahaemolyticus patogenik pada udang vaname yang berasal dari tambak tradisional dan intensif berdasarkan keberadaan gen tdh dan trh. Isolasi dan konfirmasi Vibrio parahaemolyticus mengacu pada BAM (2004), yang dilanjutkan dengan konfirmasi gen tdh dan trh Vibrio parahaemolyticus  menggunakan metode PCR.  Hasil identifikasi menunjukkan bahwa  sebanyak 16/32(50%) dan 6/32 (18,8%) udang dari tambak tradisional dan intensif positif Vibrio parahaemolyticus. Berdasarkan gen tdh, ditemukan Vibrio parahaemolyticus patogenik pada udang tambak tradisional dan intensif berturut-turut adalah 81% (13/16) dan 50% (3/6). Sementara itu, jika didasarkan pada gen trh, Vibrio parahaemolyticus patogenik pada udang tambak tradisional dan intensif berturut-turut adalah 15/16 (93,8%) dan 4/6 (66,7%). Secara keseluruhan prevalensi udang vaname yang positif gen tdh adalah sebesar 72,2% (16/22) gen trh sebesar 86,4% (19/22) dan yang memiliki kedua gen adalah sebanyak 63,6% (14/22).
Pola Pertumbuhan Listeria Monocytogenes Selama Fermentasi Tempe Yang Diperkaya Lactobacillus Fermentum Suci Apsari Pebrianti; Lilis Nuraida; Ratih Dewanti Hariyadi
Jurnal Fakultas Teknik Kuningan Vol. 1 No. 1 (2020): Jurnal Fakultas Teknik UNISA Kuningan
Publisher : Jurnal Fakultas Teknik Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Ditemukannya mikroba patogen Salmonella Paratyphi B pada tempe menunjukkan potensi kontaminasi patogen lain selama pembuatan tempe, termasuk Listeria monocytogenes yang umum ditemukan di lingkungan bersanitasi buruk. Terlebih, penerapan sanitasi produksi tempe di Indonesia masih sangat rendah. Pertumbuhan mikroba patogen dapat dihambat oleh bakteri asam laktat (BAL) seperti Lactobacillus fermentum. Penelitian ini bertujuan untuk mengetahui perilaku pertumbuhan Listeria monocytogenes selama fermentasi tempe yang diperkaya Lactobacillus fermentum. Pada penelitian ini dilakukan kontaminasi buatan Listeria monocytogenes sebesar 3 log CFU/g dan 5 log CFU/g pada tempe dengan dan tanpa penambahan Lactobacillus fermentum (6 log CFU/g). Metode hitungan cawan digunakan untuk menghitung Listeria monocytogenes, BAL, dan kapang, serta dilakukan pengamatan visual tempe yang dilakukan pada jam ke-0, 4, 24, 48, dan 72 fermentasi. Hasil penelitian menunjukkan bahwa pertumbuhan Listeria monocytogenes dengan kontaminasi awal 3 log CFU/g selama fermentasi tempe dapat dihambat dengan baik oleh Lactobacillus fermentum. Sebaliknya, penambahan Lactobacillus fermentum tidak mampu menghambat pertumbuhan Listeria monocytogenes dengan kontaminasi awal 5 log CFU/g yang mampu tumbuh mencapai 4.6 log CFU/g di akhir fermentasi. Kontaminasi buatan Listeria monocytogenes tidak mengganggu pertumbuhan kapang selama fermentasi dan menghasilkan tempe berkualitas baik sehingga berpotensi membahayakan konsumen karena tidak terdapat perbedaan visual dengan tempe normal.
Antisipasi terhadap Isu-Isu Baru Keamanan Pangan (The Anticipation of New Issues Food safety) Ratih Dewanti Hariyadi; Purwiyatno Hariyadi
JURNAL PANGAN Vol. 21 No. 1 (2012): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v21i1.97

Abstract

Kemunculan emerging dan kontaminan baru yang berkaitan dengan keamananpangan merupakan isu yang tidak bisa dihindari. E. coli enterohemoragik misalnya,merupakan contoh emerging baru yang menyebabkan kematian dan kerugian ekonomi di Eropa, sedangkan di Cina melamin adalah contoh mutakhir kontaminan pada susu formula bayi yang telah menyebabkan kematian dan kerugian perdagangan di dunia. Paper ini mengulas berbagai potensi emerging dan kontaminan baru yang mungkin muncul dalam sistem pangan. Tujuannya adalah untuk memberikan pemahaman mengenai emerging dan kontaminan baru tersebut sehingga kemunculannya bisa diantisipasi dengan tepat, baik oleh pemerintah emerging pangan maupun konsumen. Penggunaan kerangka kerja analisis risiko merupakan pendekatan logis yang perlu ditempuh oleh pemerintah untuk melahirkan kebijakan dan tindakan keamanan panganyang tepat untuk keperluan antisipasi tersebut. Sementara itu, emerging pangan perlu secara cermat mengkaji ulang praktek dan tindakan keamanan pangan, termasuk program dan rencana HACCP yang ada. Beberapa hal yang perlu dilakukan oleh emerging antara lain, mengevaluasi kembali spesifikasi ingriedien dan titik-titik kendali kritis, mengembangkan program pengambilan contoh dan pemantauan lingkungan, serta menyesuaikan program pengambilan contoh produk. Dalam kaitannya dengan pengendalian kontaminan yang sengaja ditambahkan (intentional contamination), rencana HACCP perlu mencakup juga bahaya-bahaya yang telah diidentifikasi pernah secara sengaja dimasukkan dalam sistem produksi, serta mengidentifikasi dan mengevaluasi bahaya-bahaya lain yang diketahui atau yang diduga bisa secara sengaja dimasukkan sistem produksi.Emerging pathogens and food contaminants important for food safety are inevitable issues. Enterohemorhagic E. coli, for example, is the latest one to cause fatality and economic loss in Europe while in China melamine is a very important adulaterant in powdered infant formula that has cost life and trade worldwide. This paper discusses the emergence of foodborne pathogens and contaminants in food system. The objective is to provide better understanding regarding the emerging foodborne pathogens and contaminants so that their emergence should be well anticipated by government, food industries as well as consumers. Use of risk analysis framework is the logical approach for government to produceappropriate food safety measures and policies. Meanwhile,food industry should take into precaution their existing food safety measures and revisit their HACCP plan. The most likely aspects to be reconsidered include re-evaluation of the specification for ingredients and critical control points, establishment of environmental monitoring and sampling, and adjustment of sampling plan. With respect to preventing intentional contaminations, HACCP plan should include hazards that may be intentionally introduced, identify and evaluate known or reasonably foreseeable hazards that may be associated with the facility. 
Production of Organic Acid and Short-Chain Fatty Acids (SCFA) from Lactic Acid Bacteria Isolate on Oligosaccharide Media Yati Maryati; Lilis Nuraida; Ratih Dewanti Hariyadi
Jurnal Kimia Sains dan Aplikasi Vol 24, No 6 (2021): Volume 24 Issue 6 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4089.044 KB) | DOI: 10.14710/jksa.24.6.213-221

Abstract

The growth of microorganisms in food, one of which is lactic acid bacteria (LAB), can produce metabolites beneficial to health. It is essential to study the results of LAB metabolism to improve the quality of a functional food product. This study aimed to evaluate the isolates Lactobacillus acidophilus FNCC 0051 and Lactobacillus rhamnosus R23 to metabolize oligosaccharides as a carbon source so that the final fermentation product can benefit health especially in lowering cholesterol. In vitro testing was carried out on MRS media with or without oligosaccharides, either singly or in a combination consisting of galactooligosaccharides (GOS), fructooligosaccharides (FOS), inulin (IN), inulin hydrolyzate (HI), or their combination as prebiotics by adding 0.3 % oxbile (bile salt) and inoculated with 1% v/v LAB isolate culture and incubated at 37°C for 24 hours. The results showed that the main product of oligosaccharide metabolism by L. acidophilus FNCC 0051 and L. rhamnosus R23 produced several organic acids (lactic acid), including short-chain fatty acids (SCFA) (acetic acid, propionic acid, and butyric acid). The single and combined carbon sources affected the proportion of lactic acid and acetic acid produced by L. acidophilus FNCC0051 (p<0.05). However, they did not affect the proportions of propionic acid and butyric acid. While in L. rhamnosus R23 (p<0.05), the presence of a single carbon source significantly affected the proportions of lactic acid, acetic acid, propionic acid, and butyric acid, while the combination of oligosaccharides affected the proportions of lactic acid and butyric acid produced. SCFA is the main product of prebiotic metabolism, but the characteristics of the acid produced have not been identified. The fermentation pattern is thought to be related to molecular weight, chain length, and oligosaccharide structure. Short-chain molecules, such as FOS generally ferment more rapidly than long-chain molecules such as inulin. The results of this study indicate that both isolates can be used as probiotics in the development of symbiotic products with the addition of oligosaccharides, which have a physiological effect in lowering cholesterol levels.
Kontaminasi Staphylococcus aureus dan Bacillus cereus pada Sushi di Tingkat Ritel di Wilayah Jabodetabek: Contamination of Staphylococcus aureus and Bacillus cereus in Sushi at Retail Level in Jabodetabek Area Yusma Yennie; Ratih Dewanti-Hariyadi; Harsi Dewantari Kusumaningrum; Achmad Poernomo
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 2 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(2)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i2.42066

Abstract

Susyi adalah pangan siap konsumsi asal Jepang, merupakan kombinasi nasi yang diberi asam dan ikan mentah. Umumnya susyi terdiri dari beberapa jenis, namun terdapat dua jenis susyi yang sangat dikenal yaitu nigiri (nasi dengan ikan mentah di atasnya) dan maki (nasi dengan ikan mentah di dalamnya). Ikan dalam susyi tidak mengalami pemanasan sehingga rentan terhadap kontaminasi patogen, di antaranya Staphylococcus aureus dan Bacillus cereus. Kedua bakteri ini telah dilaporkan sebagai penyebab Kejadian Luar Biasa (KLB) di Indonesia, namun keberadaannya di dalam susyi belum pernah dilaporkan. Penelitian bertujuan untuk menentukan prevalensi dan tingkat kontaminasi S. aureus dan B. cereus pada susyi di tingkat ritel di wilayah Jabodetabek (Jakarta, Bogor, Depok, Tangerang, dan Bekasi). Sebanyak 120 sampel terdiri dari nigiri (n=57) dan maki (n=63) diperoleh dari ritel di Jabodetabek. Identifikasi dilakukan dengan metode Polymerase Chain Reaction (PCR), dengan target gen nuc (S. aureus) dan gyrB (B. cereus). Jumlah bakteri menggunakan metode lempeng sebar (spread plate) untuk S. aureus dan Angka Paling Mungkin (APM) untuk B. cereus. Prevalensi S. aureus (7,5%) di dalam susyi lebih besar dibandingkan B. cereus (5%). Tingkat kontaminasi S. aureus (8,9x102-1,5x105 koloni/g) juga lebih tinggi dibandingkan B. cereus (9,2-93 APM/g). S. aureus lebih sering ditemukan dalam maki (9,5%), sementara B. cereus lebih banyak ditemukan pada nigiri (7,1%). Susyi dengan ikan salmon memiliki prevalensi S. aureus (9,3%) dan B. cereus (5,3%) lebih tinggi dibandingkan dengan tuna. Kontaminasi S. aureus kemungkinan karena kurang terpenuhinya higiene personal, sementara B. cereus dapat berasal dari penanganan nasi yang kurang baik dan sanitasi lingkungan yang kurang memadai. Keberadaan S. aureus di dalam susyi lebih berpotensi menyebabkan risiko keracunan pangan dibandingkan B. cereus. Program higiene personal dalam produksi susyi harus ditingkatkan untuk memberi jaminan keamanan.
Co-Authors - Suliantari Abd. Rahman As-syakur Achmad Poernomo Achmad Poernomo Achmad Poernomo Alifia, Murninur Amiroh Amiroh Amiroh Amiroh, Amiroh Arimah Arimah Ayu Intan Sari Aziz, Kholifatul Azura Ulfa, Azura Bambang Trisakti Budi Nurtama C. C. Nurwitri Cahyadi, Muhammad Cynthia Cynthia Cynthia CynthiaCynthia David Yudianto Dedi Fardiaz Ekawati Purwijantiningsih Ekawati Purwijantiningsih, Ekawati Eko Hari Purnomo Endang Tri Rahayu, Endang Tri Ermi Sukasih Estuningsih - Estuningsih - Ety Parwati Fadhina, Maisharah Fenny Larasati Firman Fajar Perdhana Gathot Winarso, Gathot Gino Nemesio Cepeda Hanifaturahmah, Fadiyah Harsi D. Kusumaningrum Hesty Nur Fadia Istiana (alm.) Istiana Istiana, Istiana (alm.) Ita Carolita, Ita Joko Riyanto Karina Nola Sinamo Komala, Kemal Lendrawati Lendrawati Lilis Nuraida Maggy T. Suhartono Maggy Thenawidjaja Suhartono Mala Nurilmala Melati, Rahadina Praba Muhammad Nur Mutaqin, Muhammad Zakki Nandika, Muhammad Rizki Nanin Anggraini, Nanin nFN Setyadjit Ni Gusti Ayu Made Widyatari Asthiti Ni Made Vina Citanirmala Nugroho Indrotristanto Nur Richana Nur Wulandari Nur Wulandari Nuri Andarwulan Nurwitri, C. C. Oryssa Sathalica Pradianti Pawestri, Wari Prasetio, Wiji Pratiwi Yuniarti Martoyo Pratiwi Yuniarti Martoyo Purwiyatno Hariyadi Rahmawati Rahmawati Raini Panjaitan Ramadhani Meutia, Yuliasri Ratu Ayu Dewi Sartika Rika Puspitasari MZ Rinto . Sari, Ratna Nurmalita SEDARNAWATI YASNI Shanti Emawati Siti Nurjanah Siti Nurjanah Soegiarto, Salma Rachmanda Sri Estuningsih Sri Harini Suardana, A. A. Md. Ananda Putra Suci Apsari Pebrianti Sudiyono Sudiyono Sukarno Sukarno Supar, Supar Sutrisno Hadi Purnomo Tatik Kartika, Tatik Tri Isti Rahayu Ulfah Amalia Uswatun Hasanah Vanessa Len Cahya Agustine Wati, Ari Kusuma Wijaya, Agung Dwi Wilis, Nabila Sekar Winiati P Rahayu Winiati P Rahayu Winiati P. Rahayu Winiati Pudji Rahayu Wiweka, Toh Jaya Yati Maryati Yati Maryati Yesica M. R. Sinaga Yesica Marcelina Romauli Sinaga Yuli Yanti Yuliasri Ramadhani Meutia Yusma Yennie Yusma Yennie