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ISOLATION AND IDENTIFICATION OF MICROORGANISMS DURING SPONTANEOUS FERMENTATION OF MAIZE [Isolasi dan Identifikasi Mikroorganisme pada Fermentasi Spontan Jagung] . Rahmawati; Ratih Dewanti -Hariyadi; Purwiyatno Hariyadi; Dedi Fardiaz; Nur Richana
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.448 KB) | DOI: 10.6066/jtip.2013.24.1.33

Abstract

Maize was traditionally the second most common staple food in Indonesia. Conversion to maize flour has been accomplished to improve its convenience. Traditionally, maize flour is produced by soaking the kernels in water followed by grinding. It was reported that final physicochemical characteristics of the maize flour were influenced by spontaneous fermentation which occurred during soaking. This research aimed to isolate and identify important microorganisms that grew during fermentation thus a standardized starter culture can be developed for a more controlled fermentation process. Soaking of maize grits was conducted in sterile water (grits:water=1:2, w/v) in a closed container at room temperature (±28ºC) for 72 hours. After 0, 4, 12, 24, 36, 48, 72 hours, water and maize grits were sampled and tested for the presence of mold, yeast, and lactic acid bacteria (LAB). Isolates obtained from the spontaneous fermentation were reinoculated into the appropriate media containing starch to observe their amylolytic activity. Individual isolate was then identified; mold by slide culture method, while yeast and LAB by biochemical rapid kits, i.e. API 20C AUX and API CH50, respectively. The number of each microorganism was plotted against time to obtain the growth curve of the microorganisms during spontaneous fermentation. The microorganisms were identified as Penicillium chrysogenum, P. citrinum, A. flavus, A. niger, Rhizopus stolonifer, R.oryzae, Fusarium oxysporum, Acremonium strictum, Candida famata, Kodamaea ohmeri, Candida krusei/incospicua, Lactobacillus plantarum 1a, Pediococcus pentosaceus, L. brevis 1, L. plantarum 1b, and L. paracasei ssp paracasei 3. Four molds and one yeast were amylolytic while none of the LAB was capable of starch hydrolysis. The growth curve suggested that the amylolitic mold and yeast grew to hydrolyze starch during the course of fermentation, while the LABs benefited from the hydrolyzed products and dominated the later stage of the fermentation.
Penghambatan Produksi Aflatoksin dari Aspergillus flavus oleh Kapang dan Khamir yang Diisolasi dari Ragi Tempe Ekawati Purwijantiningsih; Ratih Dewanti-Hariyadi; C. C. Nurwitri; Istiana (alm.) Istiana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2874

Abstract

This research was carried out to study the inhibition of aflatoxin production of Aspergillus flavus by moulds and yeasts isolated from ragi tempe. The result showed that moulds found in the ragi tempe consisted of Rhizopus oligosporus, R. oryzae, R. cohnii, Mucor rouxii, Rhizomucor pusillus, Aspergillus niger and miselia sterilia. Yeasts found in the samples were Candida sp1, Candida sp2 and Trichosporon sp. Twelve mould, and yeast isolates were chosen to determine their ability on inhibition of aflatoxin production. Aspergillus flavus alone or in association with each of these isolates were inoculated in Potato Dextrose Broth and analysed for aflatoxin production at day 10. All combinations yielded less aflatoxin than the single culture of A. flavus. All isolates tested could inhibit aflatoxin production. The highest inhibition was shown by Candida sp1 (99,96%).
(The Effect Of Reconstitution Temperature For Local Isolates Of Enterobacter sakazakii (Cronobacter sp.) from Powdered Infant Formula and Weaning Food) Yuliasri Ramadhani Meutia; Ratih Dewanti-Hariyadi; Estuningsih -
Warta Industri Hasil Pertanian Vol 26, No 01 (2009)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4762.921 KB) | DOI: 10.32765/warta ihp.v26i01.2577

Abstract

Enterobacter sakazakii (recently know as novel genus Cronobacter sp.) is opportunistic bacteria which can cause severe meningitis in neonates. Eight isolates of E.sakazakii which previously isolated from powdered infant formula (PIF) and weaning food were tested foe their ability to survive during reconstitution with water having various temperatures, and their fate during hang time in comparison with 7 isolates previously described by Estuningsih and E. sakazakii ATCC 352/7. reconstitution with 100 C water decreased he number of bacteria of most isolates to undetectable level, while with 40 C and 4 C water did not reduce the bacterial number significantly. Using water of 70 C, reconstitution decreased the number of bacteria of 10 isolates to undectable levels; however 6 isolates survived the reconstitution temperatures. The hang time test showed that some bacteria which were not detected after reconstitution with 70 C water became detectable after 2 hours. Those surviving reconstitution with 70 C well during hang time for 2 to 8 hours.
Analisis Pemenuhan Persyaratan Subbab 103 dan 106 US FDA FSMA di Industri Minyak Cengkeh Indonesia Lendrawati Lendrawati; Ratih Dewanti Hariyadi; Nur Wulandari
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 3 No. 2 (2016): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Nowadays, intentional contamination of food product has become an additional concern in food safety management system (FSMS) such as that in the United State Food Drug Adminstration (US FDA) Food Safety Modernization Act (FSMA). Therefore, it is necessary to raise awareness and take into consideration of intentional contamination into any production chain. The purpose of this study was to provide recommendation for updating the FSMS in PT XYZ to integrate intentional contamination. The research was conducted by reviewing regulations related to the safety of clove oil as flavoring agents, identify adulterant compounds in clove oil, assessing the existing food safety management system in PT XYZ, performing gap analysis using the main provision of  FSMA pertaining hazard analysis and risk-based preventive controls (section 103) and protection against intentional adulteration (section 106) as reference, and developing recommendations for the gaps. Pertaining to section 103 and section 106 requirements, the assessment suggested that PT XYZ fullfilled 54% of the requirements i.e  52% (section 103) and 2% (section 106). Education to raise employee awareness and develop a food defense plan are recommended to meet the FSMA requirement.
Ketahanan dan Kulturabilitas Cronobacter Sakazakii terhadap Stres Kering pada Simulasi Proses Pengeringan Siti Nurjanah; Ratna Nurmalita Sari; Ratih Dewanti-Hariyadi
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 4 No. 2 (2017)
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Cronobacter sakazakii is an opportunistic pathogen that has been isolated from several dried food products and reported can survive in dry conditions. Survival on dry stress can be observed using microscopy due to losses of their culturability in the medium. This study aimed to observe the survival and culturability of C. sakazakii on dry stress and ability to recover their culturability or resuscitation. This research was carried out in 3 steps: drying cells by desiccation, survival cell enumeration, and resuscitation. The bacteria used were two isolates Green fluorescence protein (pGFPuv) mutants of C. sakazakii, namely E2 and Yrt2a, that have ampicillin resistance and similar growth pattern with their wild-type. Desiccation was conducted by placed bacterial cells in incubator at 30, 35, 400 and 500C for 2 hours and air-drying stored at 210C for 72 hours. The culture able cells were enumerated on tryptone soy agar (TSA) and total cells include non-culturable cells were enumerated using fluorescence microscopy. Both of C. sakazakii isolates can survive for all of treatment. Total cells of E2 and Yrt2a decreased 2-3 and 4-5 log cells respectively. Both of isolates loss their ability to grow on medium, however suplementation the medium growth using pyruvate can resuscitate their cells and recover their culturability. Resuscitation of C. sakazakii cells might be as potential risk for increasing bacterial contaminant in food.
Inaktivasi Bakteri Patogen Planktonik dan Biofilm oleh Sanitaiser Komersial Ratih Dewanti Hariyadi; Cynthia CynthiaCynthia
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 1 No. 2 (2014): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Cleaning and sanitizing are important aspects in providing quality and safe foods. In the US, contaminated equipment is the third most important factor contributing to foodborne disease outbreaks. The objective of this study was to evaluate the effectiveness of commercial sanitizers against planctonic cells and biofilms of pathogenic bacteria Salmonella Typhimurium and Staphylococcus aureus. Commercial sanitizers used in this study were single sanitizers (quaternary ammonium, peroxy acetic acid, coconut fatty acid) and combined sanitizers (Oxyquart®, Bioclean 520®, Bioclean 540®) at the recommended application concentrations. Of the sanitizers tested on planctonic cells, peroxy acetic acid at 100 ppm as well as Oxyquart® and Bioclean 540® at concentrations of 0.010% are effective against S. Typhimurium; while Oxyquart® at a concentration of 0.005% is the most effective sanitizer against S. aureus. The study also shows that Oxyquart® is more effective against biofilms and capable of inactivating 3.94-5.02 log CFU/cm2 of S. Typhimurium or S. aureus biofilm. 
Kajian Penerapan Peraturan Menteri Pertanian Nomor 53 Tahun 2012 untuk Pengendalian Aflatoksin pada Pala Ni Made Vina Citanirmala; Winiati Pudji Rahayu; Ratih Dewanti Hariyadi
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 3 No. 1 (2016): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Nutmeg as Indonesia main exported commodity frequently contaminated by aflatoxin and often rejected by European Union. To improve safety of nutmeg, Ministry of Agricultural (MA) issued a regu- lation number 53 in 2012 regarding good practices on nutmeg. The objectives of this research were to (1) evaluate implementation of the MA regulation at nutmeg supply chain in Siau Barat District (2) identify critical points for mold growth and/or aflatoxin formation along the nutmeg supply chain. This research conducted through (1) surveys to 60 farmers, 10 middlemen, 2 exporters and 3 government officials as respondents; (2) identification of critical steps using HACCP decision trees. The evaluation of MA regu- lation showed that at farmer level was poor for postharvest handling (54.4%) caused by lack of facilities and infrastructure (57.2%), although for harvest was moderate (70.4%). At the middleman level, handling practices at postharvest was moderate (62.5%), although not supported with facilities and infrastructure (36.7%). Handling practices at exporter level was generally good, however supervision at government official level was poor (56.3%). Critical control points determined at farmer level were harvesting and drying, at middlemen level were receiving, drying and storage, while at exporter level were receiving and shipment. 
Pengembangan Sistem Cara Produksi Makanan Enteral yang Baik (CPMEB) dan Aplikasinya di Rumah Sakit Amiroh Amiroh; Winiati Pudji Rahayu; Ratih Dewanti Hariyadi
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 3 No. 1 (2016): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Hospital formula enteral food is a special food targeted specifically for a group of people with health risk. Therefore its safety needs to be controlled more stringently for example through the application Good Manufacturing Practices (GMP). Presently, the guidelines for good processing practices for enteral food is not available yet. This research aims to develop a Good Enteral Food Manufacturing Practices (GEFM) consisting of a guideline as well as a sysstematic audit system that can be applied easily in the hospital. The system was developed based on the Ministry of Health of Republic of Indonesia Regulation Number:1096/MenKes/PER/VI/2011 on hygiene and sanitation for food service industries and other relevant references. Based on the literature review and trials, thirteen aspects were defined for the require- ments; including four main aspects pertaining production room, production equipment, process control and workers' hygiene. Other aspects include building and its facilities, sanitation facility, raw materials storage, monitoring, pest control, distribution, training, patient feeding, and documentation. An audit trial at a hospital Y suggested that its enteral food production unit was categorized as good; but needed improve- ment in several aspects such as: production equipment storage, hygiene and sanitation maintenance, food distribution and food handle training. 
Kajian Kesesuaian Standar Cemaran Kimia (Logam Berat dan PAH) pada Produk Perikanan di Indonesia dengan Standar Negara Lain dan Codex Oryssa Sathalica Pradianti; Winiati Pudji Rahayu; Ratih Dewanti- Hariyadi
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): Juni 2019
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v14i1.560

Abstract

AbstrakPangan dapat terkontaminasi oleh cemaran kimia karena penanganan dan pengolahan pangan yang tidak sesuai. Tujuan dari penelitian ini adalah 1) mengidentifikasi mayoritas penyebab penolakan produk perikanan Indonesia, 2) menelaah standar cemaran kimia pada produk perikanan, khususnya logam berat yang ada di Indonesia, Codex  Alimentariurs Commision (CAC) dan negara-negara lain, serta 3) memberikan rekomendasi bagi pemerintah selaku regulator dalam proses perumusan suatu standar. Dokumen standar cemaran kimia pada produk perikanan dikumpulkan dari dokumen/peraturan yang dikeluarkan oleh Badan Pengawasan Obat dan Makanan (BPOM), Badan Standardisasi Nasional (BSN), CAC, dan 11 negara lain yaitu Uni Eropa, Kanada, China, Korea Selatan, Vietnam, Amerika Serikat, Jepang, Malaysia, Singapura, Thailand, dan Australia. Hasil penelitian menunjukkan bahwa dari 164 notifikasi penolakan produk perikanan Indonesia di Uni Eropa, Kanada, dan Korea Selatan selama 10 tahun (2008-2017), penolakan produk perikanan tertinggi disebabkan oleh adanya cemaran kimia merkuri dan metilmerkuri pada ikan todak sebesar 27%, kadmium pada gurita sebesar 5% dan benzo[a]piren pada ikan asap sebesar 3%. Batas maksimum cemaran kimia untuk arsen, kadmium, dan timbal (pada ikan predator) di Indonesia yang terdapat pada Peraturan Kepala (Perka) BPOM Nomor 5 Tahun 2018 lebih rendah dibandingkan dengan yang terdapat di SNI 7387:2009 maupun yang ditetapkan oleh CAC serta negara lain. Indonesia telah menetapkan batas maksimum benzo[a]piren pada ikan asap, sementara itu CAC hanya menetapkan code of practice terhadap benzo[a]piren. Peraturan cemaran logam berat belum sepenuhnya dipedomani oleh para eksportir sehingga masih terdapat penolakan produk perikanan Indonesia. Hal ini menunjukkan masih perlu dilakukan pengawasan terkait kandungan logam berat yang terdapat pada produk perikanan di Indonesia. Code of practice terkait proses pengolahan pangan direkomendasikan untuk diterbitkan guna meminimalisir kandungan benzo[a]piren. Compliance Assessment of Chemical Contaminant Standard (Heavy Metal and PAH) for Fishery Products in Indonesia with those of Other Countries and CodexAbstractFood can be contaminated by chemical contamination through inappropriate food handling and processing. The purpose of this study aims to: 1) identify the chemical contamination caused majority of the rejection of Indonesia fishery products, 2) reviewing the chemical standards of contamination fishery products in Indonesia, Codex Alimentariurs Commision (CAC) and other countries, and 3) provide recommendations for the setting of chemical contamination standards in fishery products. Chemical contamination standards were collected from regulations issued by the Indonesia National Agency of Drug and Food Control (NADFC), National Standardization Agency of Indonesia, CAC, and 11 other countries: European Union/EU, Canada, China, South Korea, Vietnam, United States of America, Japan, Malaysia, Singapore, Thailand, and Australia. The results showed that in the 10 years period (2008-2017), there were 164 rejection notifications in EU, Canada, and South Korea and the highest rejection was caused by chemical contamination i.e. 27% caused by mercury and methylmercury in swordfish, 5% caused by cadmium in octopus and 3% caused by benzo[a]pyrene in smoked fish. The maximum limit of chemical contamination for arsenic, cadmium, and lead (predatory fish) in Indonesia as stated in the Regulation of the Head of NADFC Number 5 of 2018 lower than the maximum limit set in SNI 7387: 2009 and sets in CAC and other countries. Indonesia set the maximum limit for benzo[a]pyrene in smoked fish, whereas CAC published the code of practice to avoid a benzo[a]pyrene formation. Regulations of maximum limit for heavy metal contamination have not been fully guided by fisheries exporters, so that there was still rejection of Indonesian fishery products. The action needed is tightening the monitoring of heavy metal in fishery products in Indonesia. Code of practice to avoid benzo[a]pyrene formation is recommended to set up to minimize the benzo[a]pyrene formation on food processing.    
Prevalensi Gen tdh dan trh Vibrio parahaemolyticus pada Udang Vaname Di Wilayah Indramayu, Jawa Barat Yusma Yennie; Ratih Dewanti Hariyadi; Achmad Poernomo
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): Juni 2015
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v10i1.245

Abstract

Penelitian ini bertujuan untuk mengetahui prevalensi Vibrio parahaemolyticus patogenik pada udang vaname yang berasal dari tambak tradisional dan intensif berdasarkan keberadaan gen tdh dan trh. Isolasi dan konfirmasi Vibrio parahaemolyticus mengacu pada BAM (2004), yang dilanjutkan dengan konfirmasi gen tdh dan trh Vibrio parahaemolyticus  menggunakan metode PCR.  Hasil identifikasi menunjukkan bahwa  sebanyak 16/32(50%) dan 6/32 (18,8%) udang dari tambak tradisional dan intensif positif Vibrio parahaemolyticus. Berdasarkan gen tdh, ditemukan Vibrio parahaemolyticus patogenik pada udang tambak tradisional dan intensif berturut-turut adalah 81% (13/16) dan 50% (3/6). Sementara itu, jika didasarkan pada gen trh, Vibrio parahaemolyticus patogenik pada udang tambak tradisional dan intensif berturut-turut adalah 15/16 (93,8%) dan 4/6 (66,7%). Secara keseluruhan prevalensi udang vaname yang positif gen tdh adalah sebesar 72,2% (16/22) gen trh sebesar 86,4% (19/22) dan yang memiliki kedua gen adalah sebanyak 63,6% (14/22).
Co-Authors - Suliantari Abd. Rahman As-syakur Achmad Poernomo Achmad Poernomo Achmad Poernomo Amiroh Amiroh Amiroh Amiroh, Amiroh Arimah Arimah Ayu Intan Sari Aziz, Kholifatul Azura Ulfa, Azura Budi Nurtama C. C. Nurwitri Cahyadi, Muhammad Cynthia Cynthia Cynthia CynthiaCynthia David Yudianto Dedi Fardiaz Ekawati Purwijantiningsih Ekawati Purwijantiningsih, Ekawati Eko Hari Purnomo Endang Tri Rahayu, Endang Tri Ermi Sukasih Estuningsih - Estuningsih - Fadiyah Hanifaturahmah Fenny Larasati Firman Fajar Perdhana Gathot Winarso, Gathot Gino Nemesio Cepeda Harsi D. Kusumaningrum Hesty Nur Fadia Istiana (alm.) Istiana Istiana, Istiana (alm.) Joko Riyanto Karina Nola Sinamo Komala, Kemal Lendrawati Lendrawati Lilis Nuraida Maggy T. Suhartono Maggy Thenawidjaja Suhartono Mala Nurilmala Muhammad Nur Mutaqin, Muhammad Zakki Nandika, Muhammad Rizki Nanin Anggraini, Nanin nFN Setyadjit Ni Gusti Ayu Made Widyatari Asthiti Ni Made Vina Citanirmala Nugroho Indrotristanto Nur Richana Nur Wulandari Nur Wulandari Nuri Andarwulan Nurwitri, C. C. Oryssa Sathalica Pradianti Pawestri, Wari Prasetio, Wiji Pratiwi Yuniarti Martoyo Pratiwi Yuniarti Martoyo Purwiyatno Hariyadi Rahadina Praba Melati Rahmawati Rahmawati Raini Panjaitan Ramadhani Meutia, Yuliasri Ratu Ayu Dewi Sartika Rika Puspitasari MZ Rinto . Sari, Ratna Nurmalita SEDARNAWATI YASNI Shanti Emawati Siti Nurjanah Siti Nurjanah Soegiarto, Salma Rachmanda Sri Estuningsih Suardana, A. A. Md. Ananda Putra Suci Apsari Pebrianti Sudiyono Sudiyono Sukarno Sukarno Supar, Supar Sutrisno Hadi Purnomo Tri Isti Rahayu Ulfah Amalia Uswatun Hasanah Vanessa Len Cahya Agustine Wati, Ari Kusuma Wijaya, Agung Dwi Wilis, Nabila Sekar Winiati P Rahayu Winiati P Rahayu Winiati P. Rahayu Winiati Pudji Rahayu Wiweka, Toh Jaya Yati Maryati Yati Maryati Yesica M. R. Sinaga Yesica Marcelina Romauli Sinaga Yuli Yanti Yuliasri Ramadhani Meutia Yusma Yennie Yusma Yennie