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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrointek Jurnal Penelitian Pascapanen Pertanian agriTECH Biota Warta IHP (Warta Industri Hasil Pertanian) Caraka Tani: Journal of Sustainable Agriculture Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Forest and Society Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL PANGAN Jurnal Mutu Pangan : Indonesian Journal of Food Quality Budimas : Jurnal Pengabdian Masyarakat SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Jurnal Fakultas Teknik UNISA Kuningan Journal of Livestock and Animal Health (JLAH) Food and Agro-industry (FAGI) Journal Food and Agro-industry (FAGI) Journal Jurnal Standardisasi

Ratih Dewanti -Hariyadi

Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus IPB, Darmaga, Jl. Lingkar Akademik, Babakan, Kec. Dramaga, Bogor, Jawa Barat 16680

Author-ID : 209263

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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RAPID DETECTION OF Salmonella IN SHRIMP BY POLYMERASE CHAIN REACTION [Deteksi Cepat Salmonella pada Udang dengan Polymerase Chain Reaction] Ulfah Amalia; Ratih Dewanti-Hariyadi; Achmad Poernomo
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

RAPID DETECTION OF Salmonella IN SHRIMP BY POLYMERASE CHAIN REACTION [Deteksi Cepat Salmonella pada Udang dengan Polymerase Chain Reaction]Ulfah Amalia1,2), Ratih Dewanti-Hariyadi2,3)* and Achmad Poernomo4)1) Faculty of Fisheries and Marine Science, Diponegoro University, Semarang2) Department of Food Science and Technology, Bogor Agricultural University, Bogor3) Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center, Bogor Agricultural University, Bogor4) Center for Research and Development and Product Processing and Marine Biotechnology Fisheries (CRDPPMBF), the Ministry of Marine Affairs and Fisheries, Jakarta Accepted September 27th 2013 / Approved July 4th 2014ABSTRACT Shrimp is an important non-oil commodity for foreign trade in Indonesia. However, rejection of shrimp exports by the importing countries is still commonly encountered. In 2011, the USFDA recorded two cases of Salmonella spp. contamination in shrimp products from two shrimp processing companies in Indonesia. Analysis of Salmonella spp. in seafood is generally performed using a conventional method which takes at least 5 days. The objective of the study is to get a Salmonellae rapid detection method in shrimp by PCR. In this study, optimization of PCR protocol method to detect Salmonella invA gene was conducted using six different annealing temperatures (59, 59.5, 60.8, 62, 64 and 64.5°C). The results showed that 64°C was the optimum annealing temperature to detect the 284 bp fragment of Salmonella invA gene. The PCR based detection method has a DNA detection limit of 27.81mg/mL and 10°CFU/mL of viable salmonellae with 100% specificity. The PCR protocol is capable of detecting six different Salmonella serovars (S. Enteritidis, S. Hadar, S. Heidelberg, S. Kentucky, S. Paratyphi and S. Typhimurium) but none of the non salmonellae isolates. Application of the PCR assay on Salmonella in shrimp after the selective enrichment step suggested that all 16 samples were positive for Salmonella. At the same time, the conventional method could only detected 3 samples for Salmonella positive.

Cronobacter sakazakii MEMASUKI KONDISI VIABLE BUT NONCULTURABLE SELAMA PEMBENTUKAN BIOFILM Yesica M. R. Sinaga; Ratih Dewanti-Hariyadi; - Suliantari
Jurnal Teknologi dan Industri Pangan Vol. 27 No. 2 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Studies show that nonsporeformer food-borne pathogens may enter a viable but nonculturable (VBNC) state under stress conditions. This research aimed to study the ability of Cronobacter sakazakii to enter a VBNC state during biofilm formation on stainless steel (SS) surfaces and its resuscitability. C. sakazakii YRt2a pGFPuv mutant and wildtype (WT) originally isolated from powder infant formula (PIF) were used in this study. Biofilms were developed on SS surfaces in 1/10 Trypticase Soy Broth (TSB). Culturability of the biofilms was monitored by swabbing and plating the WT or mutant sessile cells onto Trypticase Soy Agar (TSA) or TSA containing 100 μg/mL ampicillin (TSAA), respectively. Meanwhile, their viability was measured using direct microscopic (DMC) count based on green fluorescence for mutant isolates and direct viable count (DVC) for the WT using a fluorescence microscope. Biofilm of C. sakazakii pGFPuv mutant on SS entered VBNC state after 25 days of incubation, while the WT C. sakazakii biofilms was still culturable until day 63. Sodium pyruvate in solid and liquid medium was not able to resuscitate the biofilm cells of C. sakazakii pGFPuv in VBNC state. C. sakazakii pGFPuv mutants enter VBNC state faster than the WT isolates. Depleted nutrient is thought to drive biofilm of C. sakazakii pGFPuv to enter VBNC.

SINTASAN Cronobacter sakazakii pGFPuv SELAMA PENYIMPANAN JAGUNG PIPILAN BERKADAR AIR AWAL BERBEDA DI BERBAGAI RH Karina Nola Sinamo; - Suliantari; Ratih Dewanti-Hariyadi
Jurnal Teknologi dan Industri Pangan Vol. 27 No. 2 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Cronobacter sakazakii is an opportunistic foodborne pathogen reported to cause necrotizing enterocolitis, bacteremia, and meningitis in certain groups of infant. C. sakazakii has been reported to survive at low aw or dryness. Presence of wild-type C. sakazakii in dry product is difficult to be distinguished from naturally occuring C. sakazakii. A pGFPuv mutant of C. sakazakii has been reported to have similar growth pattern, thus has the potential to be used in further investigation. The study aimed to evaluate the effect of initial moisture content and relative humidity (RH) on the survival rate of C. sakazakii pGFPuv in corn kernels during storage at room temperature. The study consists of drying corn kernels to achieve moisture contents of 12 and 16% (w.b), inoculation of C. sakazakii pGFPuv, and storage at RH 50, 70 and 90% for 12 weeks. Every two week, corn kernels were sampled and the moisture content was measured using oven method, water activity was measured with aw meter, and total C. sakazakii pGFPuv was enumerated by spread plate method. Meanwhile, total bacteria, mold and yeast were enumerated by pour plate method. Corn kernels achieved equilibrium moisture content and aw after two weeks of storage. The number of C. sakazakii decreased rapidly during storage at RH 70 and 90%, however they could survived at RH 50% for 12 weeks, especially when the initial moisture content was 16%. The total bacteria decreased by 3.5-3.9 Log CFU/g during storage at three RHs, but mold and yeast increased rapidly at RH 90%.

SELEKSI ISOLAT BAKTERI ASAM LAKTAT ASAL TEMPE DAN TAPE SEBAGAI KANDIDAT PROBIOTIK Raini Panjaitan; Lilis Nuraida; Ratih Dewanti-Hariyadi
Jurnal Teknologi dan Industri Pangan Vol. 29 No. 2 (2018): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Lactic acid bacteria (LAB) have been isolated from Indonesian fermented foods such as tempe and tape. Some lactic acid bacteria are known to have health benefits and as probiotics. The objective of this study was to evaluate the probiotic potential of lactic acid bacteria isolated from tempe and tape. LAB isolated from tape were evaluated for their tolerance towards low pH (pH 2.0) and 0.5% bile salt. LAB isolated from tape and tempe resistant to low pH and bile salt were then evaluated for their antimicrobial activities against pathogenic bacteria (E. coli ATCC 25922, S. typhimurium ATCC 14028, L. monocytoge-nes ATCC 7644, S. aureus ATCC 25923 and B. cereus ATCC 11778). The isolates were also tested for their adherence properties, consisting of hidrophobicity test using microbial adhesion to solvents (MATS) assay, autoaggregation as well as coaggregation between LAB and pathogens. The results of this study indicated that three isolates from tape (L. fermentum 1 BK2-5, L. fermentum 2 BK2-7, P. acidilactici NG6-4) showed good tolerance to pH 2.0 and 0.5% bile salt. L. fermentum S21209 isolated from tempe showed the strongest antimicrobial activity against the five pathogens tested. Based on the adhesion to xylene, L. fermentum 1 BK 2-5 was categorized as strong hydrophobic, followed by L. fermentum S21209 and L. fermentum TS4-5. Meanwhile, L. fermentum TS 4-5, L. fermentum S21209, L. fermentum 1 BK 2-5 and P. pentose-ceus 1 W2SR04 have the highest autoaggregation ability. L. fermentum S21209 also had the highest co-aggregation ability to the five pathogenic bacteria tested. The ability of LAB to coaggregate with all pathogens tested correlated with their strong antimicrobial properties. Based on the resistance toward low pH and bile salt, antimicrobial activity and adherence properties, L. fermentum S21209 from tempe and L. fermentum 1 BK 2-5 from tape are the most potential as probiotic candidates.

SIMPLEKS DAN MULTIPLEKS PRE-ENRICHMENT-PCR UNTUK DETEKSI Salmonella Enteritidis DAN Typhimurium PADA KARKAS AYAM Siti Nurjanah; Winiati P. Rahayu; Ratih Dewanti-Hariyadi; Ni Gusti Ayu Made Widyatari Asthiti; Rahadina Praba Melati
Jurnal Teknologi dan Industri Pangan Vol. 32 No. 2 (2021): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2021.32.2.148

A PCR assay has been developed and applied to detect Salmonella contamination in chicken carcasses. However, a concentration fewer than 3 cells per gram lead to false-negative results due to difficulties in the DNA extraction. The objective of this study was to evaluate of the influence of pre-enrichment on the sensitivity of simplex and multiplex PCR methods the detection of for Salmonella spp., S. Enteritidis and S. Typhimurium in chicken carcasses. Artificial contamination was done using very low number of Salmonella Hadar, S. Enteritidis dan S. Typhimurium and pre-enrichment was carried out by 8 hours incubation in non-selective (BPW) medium. The results showed that simplex PCR could detect Salmonella spp., S. Enteritidis and S. Typhimurium at initial numbers of 2.3, 0.9 and 2.3 MPN/mL of cells in broth medium, respectively. A multiplex PCR could detect mixed culture of the three Salmonella serovars at an initial number of 0.73 MPN/mL of cells. When compared to non-enrichment treatment, simplex pre-enrichment-PCR gave an increase in the percentage of positive results in chicken carcasses (n= 12), from 75 to 100% for Salmonella spp., from 8 to 58% for S. Typhimurium, and from 58 to 75% for S. Enteritidis. Increasing in the positive percentage was also occurred at multiplex pre-enrichment-PCR, however the concentration of S. Enteritidis primer was not optimum for detection. Pre-enrichment step significantly increases the sensitivity of PCR-based assay for detection Salmonella.

KINERJA METODE CEPAT UNTUK EVALUASI KEBERSIHAN PERALATAN PRODUKSI PANGAN Vanessa Len Cahya Agustine; Siti Nurjanah; Ratih Dewanti Hariyadi
AGROINTEK Vol 15, No 3 (2021)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v15i3.7066

Sanitation programs for equipment in food production are designed to reduce the number of microorganisms. Food industries verify the fullfilment of sanitation programs using various methods. This study aimed to evaluate the performance of several methods for evaluate of cleanliness of food production equipment. Escherichia coli, Klebsiella and protein residues that were artificially contaminated on stainless steel plates and the number of bacteria and or protein were analyzed using microbiological method with chromogenic media EB, ATP method with bioluminesence assay and protein method with semiquantitative method. The sensitivity and correlation of each method was measured using statistical data. The results showed that the chromogenic media EB and ATP bioluminesence were able to detect the contamination of Escherichia coli and Klebsiella at the lowest concentration of 1 log CFU. The result using chromogenic media EB showed correlation values were 0.9846 and 0.9946, while ATP method showed the correlation value is 0.9785 and 0.9681. ATP and protein method are able to detect protein at high concentrations, but ATP method is positively correlated to the amount of protein residue with a correlation value is 0.884. Concluded that the chromogenic media EB, ATP and protein method able to detect contamination on equipment with fullfilment the performance evaluation.

Analisis Kecukupan Panas Pada Proses Pasteurisasi Puree Mangga (Mangifera Indica L) Ermi Sukasih; nFN Setyadjit; Ratih Dewanti Hariyadi
Jurnal Penelitian Pascapanen Pertanian Vol 2, No 2 (2005): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v2n2.2005.56-65

Selama ini suhu dan waktu pasteurisasi puree mangga masih mengacu pada produk lain karena belum ada data tentang suhu dan waktu yang optimal pasteurisasi puree mangga sehingga perlu dilakukan penelitian untuk menghitung kecukupan panasnya. Penelitian ini bertujuan untuk menguji ketahanan panas mikroba penyebab kerusakan puree mangga melalui penghitungan nilai 0 (waktu yang diperlukan untuk mereduksi mikroba sebesar satu siklus log pada suhu tertentu, nilai z (perubahan suhu yang menyebabkan reduksi mikroba sebesar satu nilai D) dan menghitung nilai P (kecukupan panas pada proses pasteurisasi). Uji ketahanan panas dilakukan terhadap isolat mikroba dari puree mangga yang telah rusak yaitu isotat bakteri A, isolat bakteri B, isolat khamir C, isolat khamir E, isolat kapang G dan isolat kapang H dengan media puree mangga yang disterilisasi pada suhu 121oC selama 15 menit. Selain itujuga dilakukan terhadap mikroba populasi alami dalam puree Illangga yaitu dengan memanaskan puree mangga dan menghitung jumlah mikrobanya. Metode yang digunakan adalah metode tabung pada kombinasi suhu pemanasan 55,65,75 dan 85oC selama 0, 5,10, 15 dan 20 menit dengan tiga kali ulangan. Parameter yang diamati adalah jumlah mikroba akhir setelah pemanasan. I-lasil penelitian menunjukkan bahwa isolat bakteri A dengan nilai z sebesar 21 ,23oC mempunyai ketahanan panas yang lebih tinggi dari isolat bakteri B dengan nilai z sebesar 15,04oC, isolat khamir C dengan nilai z sebesar 24,39oC mempunyai ketahanan panas lebih tinggi dari isolat khamir E dengan nilai z sebesar 20,28oC, dan isolat kapang G dengan nilai z sebesar 18,76oC mempunyai ketahanan panas lebih tinggi dari kapang H dengan nilai z adalah 17,48oC. Sementara itu mikroba tunggal hasil isolasi mempunyai ketahanan panas lebih rendah daripada populasi mikroba yang secara alami terdapat pada puree mangga. Dari uji ketahanan panas diperoleh bahwa mikroba yang paling tahan panas adalah bakteri populasi alami dengan nilai z sebesar 52,91oC, dan ini digunakan untuk menghitung nilai P. Berdasarkan perhitungan diperoleh bahwa nilai P puree mangga adalah 15,5 menit. Hal ini berarti bahwa puree mangga akan tercapai kecukupan panasnya bila dipasteurisasi pada kombinasi suhu dan waktu yang mempunyai nilai P sebesar 15,5 menit. Heat adequacy analysis of mango puree pasteurization process The optimum lime and temperature for pasteurization of mango puree is still unavailable, so it requires to do research on this area. Determination of heat resistant microorganism isolated from rotten mango puree was conducted to determine the D, z and P value. Isolated microbes used were bacteria A, bacteria B, yeast C, yeast E, mold G, mold H and they were inoculated into sterilized mango puree. In addition, the nonsterilized mango puree containing mixture of unknown microorganisms was also evaluated for its heat resistance. Mango puree was pasteurised at 55, 65, 75 and 85oC for 5, 10, 15 and 20 minutes. The results showed that bacteria A with z value of 21.23oC has higher heat resistant than bacteria B with z value of 15.04oC, yeast C with z value of 24.39oC has higher heat resistant than yeast E with z value of 20.28oC and mold G with z value of 18.76oC has higher heat resistant than mold H with z value of 17,48oC. The single microorganism isolated has lower heat resistant than the natural population microorganism in mango puree. The natural population bacteria of mango puree has highest heal resistant with z value of 52.91 oC. This value was used to determine of P value. The result was 15.5 minutes, which means that mango puree will achieve adequate heal treatment if it is pasteurized at time and temperature which has P value of 15.5 minutes.

Isolasi dan Identifikasi Bakteri Asam Laktat Penghasil Inhibitor Enzim HMG-KoA Reduktase dari Bekasam Sebagai Agen Pereduksi Kolesterol Rinto Rinto; Ratih Dewanti; Sedarnawati Yasni; Maggy Thenawidjaja Suhartono
agriTECH Vol 35, No 3 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The purpose of this research was to obtain statins producer bacteria as a HMG-CoA reductase (HMGR) enzyme inhibitor to reduced cholesterol biosynthesis. Stages of this research were the isolation of compactin and lovastatin resistant bacteria, statin production, analysis of culture extracts to inhibition of HMG-CoA reductase and identification of bacteria. The results showed that the 20 isolates of compactin and lovastatin resistant bacteria, there are 5 bacterial isolates produced statins. They were L3.3.4; C3.4.2; C3.3.5; C3.4.4 and L3.3.3; with the statins content were 9.491; 1.536; 0.065; 0.060; and 0.040 ppm. Selection of the 5 bacterial isolates resulted 2 bacteria which had inhibition ability to HMGR enzyme activity. They were Lactobacillus acidophilus and Lactobacillus delbruckii sp. delbruckii with inhibitory ability were 66.67% and 58.33%, respectively.ABSTRAKPenelitian ini bertujuan memperoleh bakteri penghasil statin sebagai inhibitor enzim HMG-KoA reduktase (HMGR), penghambat sintesis kolesterol. Tahapan penelitian yang dilakukan adalah isolasi bakteri yang resisten terhadap compactin dan lovastatin, produksi statin, uji penghambatan ekstrak dari kultur bakteri terhadap HMG-KoA reduktase dan identifikasi bakteri. Hasil penelitian menunjukan bahwa dari 20 isolat bakteri yang resisten terhadap compactin maupun lovastatin, terdapat 5 isolat bakteri yang potensial menghasilkan statin, yaitu isolat L3.3.4; C3.4.2; C3.3.5; C3.4.4 dan L3.3.3; dengan kandungan statin berturut-turut adalah 9.491; 1,536; 0,065, 0,060, dan 0,040 ppm. Seleksi terhadap 5 isolat menghasilkan 2 bakteri yang mempunyai kemampuan penghambatan terhadap aktivitas enzim ¸Â•ÝWtu Lactobacillus acidophilus dan Lactobacillus delbruckii sp. delbruckii dengan kemampuan penghambatan berturut-turut adalah 66,67% dan 58,33%.

Kajian Isolat Bakteri Asam Laktat dalam Menurunkan Kolesterol Secara In Vitro dengan Keberadaan Oligosakarida Yati Maryati; Lilis Nuraida; Ratih Dewanti Hariyadi
agriTECH Vol 36, No 2 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

This work evaluated the abilities of five isolates of lactic acid bacteria (LAB) from different sources, i.e Lactobacillus fermentum S21209 and Lactobacillus plantarum 1-S27202 from tempe, Lactobacillus rhamnosus R23 and Pediococcus pentosaceus 1-A38 from human breast milk and a commercially available human isolates Lactobacillus acidophilus FNCC0051 in lowering cholesterol by in vitro and metabolizing the prebiotic oligosaccharide compounds. The effects of oligosaccharide compounds on the performance of the LAB isolates in lowering cholesterol were also evaluated. The tests were done in MRS based medium in vitro with or without oligosaccharides i.e. galactooligosacharrides (GOS), fructooligosaccharides (FOS), inulin, hydrolyzed inulin or combination of oligosaccharides as prebiotics. The results revealed that all isolates were able to reduce cholesterol in the medium, and the highest cholesterol reduction was observed for L. acidophilus FNCC0051 and L. rhamnosus R23. There are two different mechanism in the loweringof cholesterol; cholesterol assimilation and cholesterol binding on the cell surface. For the case of P. pentosaceus 1-A38, it involves the assimilation, while the other four isolates may involve cholesterol binding on the cell surface. In addition, the tested LAB’s has different ability to use prebiotics, as shown by the reduction of total sugar in the medium. Oligosaccharides metabolism by L. acidophilus FNCC0051 and L. rhamnosus R23 resulted in several organic acid and SCFA with lactic acid produced as the largest proportion followed by acetic acid. Furthermore, the proportion of propionic and butyric acids were influenced by the type of isolates and carbon source. L. acidophilus FNCC 0051 was able to reduce cholesterol in the MRS based medium with oligosaccharides and their combination as carbon source and cholesterol reducing ability seems to involve both assimilation and cholesterol binding on the cell surface.ABSTRAKPenelitian ini mengevaluasi lima isolat bakteri asam laktat (BAL) dari sumber yang berbeda, yaitu Lactobacillus fermentum S21209 dan Lactobacillus plantarum 1-S27202 dari tempe, Lactobacillus rhamnosus R23 dan Pediococcus pentosaceus 1-A38 dari ASI dan isolat komersial Lactobacillus acidophilus FNCC 0051 dari percernaan manusia dalam kemampuannya menurunkan kolesterol secara in vitro dan kemampuannya memetabolisme senyawa oligosakarida prebiotik. Pengaruh senyawa oligosakarida terhadap kemampuan isolat BAL terpilih untuk menurunkan kolesterol juga dievaluasi. Pengujian dilakukan pada media berbasis MRS dengan atau tanpa oligosakarida terdiri dari galaktooligosakarida (GOS), fruktooligosakarida (FOS), inulin, hidrolisat inulin atau kombinasi oligosakarida sebagai prebiotik. Hasil penelitian menunjukkan bahwa semua isolat mampu menurunkan kolesterol, dan penurunan kolesterol tertinggi ditunjukkan oleh isolat L. acidophilus FNCC0051 dan L. rhamnosus R23. Penurunan kolesterol diduga terjadi melalui dua cara yang berbeda. Mekanisme penurunan kolesterol oleh isolat P. pentosaceus 1-A38 melibatkan asimilasi kolesterol, sedangkan pada keempat isolat lainnya kemungkinan melibatkan pengikatan kolesterol pada permukaan sel. Selain itu, isolat BAL juga memiliki kemampuan yang berbeda dalam memanfaatkan oligosakarida prebiotik, terlihat pada penurunan total gula dalam medium. Metabolisme senyawa oligosakarida oleh L. acidophilus FNCC0051 dan L. rhamnosus R23 menghasilkan beberapa asam organik termasuk SCFA dengan proporsi terbesar asam laktat diikuti oleh asam asetat. Selain itu, proporsi asam propionat dan butirat dipengaruhi oleh jenis isolat dan sumber karbon. L. acidophilus FNCC 0051 mampu menurunkan kolesterol dalam media berbasis MRS dengan keberadaan oligosakarida baik tunggal maupun kombinasi sebagai sumber karbon dan melibatkan mekanisme baik asimilasi dan pengikatan kolesterol pada permukaan sel.

Prevalensi Kapang Okratoksigenik dan Kandungan Okratoksin A pada Kopi Selang Semende Rika Puspitasari MZ; Harsi Dewantari Kusumaningrum; Ratih Dewanti Haryadi
agriTECH Vol 40, No 2 (2020)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Kecamatan Semende, Kabupaten Muara Enim merupakan daerah penghasil kopi dengan produksi mencapai 92% dari komoditi hasil pertaniannya. Kopi selang adalah istilah lokal untuk kopi yang dipanen pada Desember- Juli sebelum musim panen raya. Penelitian ini bertujuan untuk mengetahui prevalensi kapang oktratoksigenik dan kandungan okratoksin A pada kopi selang Semende. Sejumlah 40 sampel kopi selang dikumpulkan dari petani (buah dan biji kopi beras), pengumpul (biji kopi beras), dan pengolah (bubuk kopi). Sampel dianalisis kadar air, mutu mikrobiologi dan kandungan okratoksin. Rata-rata kadar air buah kopi dan biji kopi asal petaniditemukan sebesar 69,88% dan 13,90%, sedangkan biji kopi asal pengumpul dan bubuk kopi asal pengolah sebesar 13,45% dan 2,97%. Kadar air biji kopi sedikit melebihi ketentuan dalam SNI yaitu 12,5%. Rata-rata angka lempeng total dan angka kapang-khamir pada bubuk kopi di tingkat pengolah adalah 1,90 Log CFU/g dan 1,99 Log CFU/g, masih memenuhi persyaratan yang berlaku untuk bubuk kopi. A. niger ditemukan pada buah kopi asal petani (50%), biji kopi asal petani (90%) maupun pengumpul (90%) dan pada bubuk kopi (30%). A. ochraceus hanya ditemukan pada biji kopi petani (10% ) dan bubuk kopi (30%). Hal ini menunjukkan bahwa kapang pencemar sudah ditemukan sejak dari tingkat petani. Walaupun demikian, kandungan okratoksin A relatif rendah dan masih memenuhi persyaratan yang ditentukan (maksimum 5 ppb). Okratoksin A pada biji kopi asal pengumpul ditemukan pada kisaran 0,86 ppb-2,81 ppb, sedangkan pada biji kopi asal petani sebesar 0,14 ppb dan pada bubuk kopi asal pengolah sebesar 0,19 ppb.

Co-Authors - Suliantari Abd. Rahman As-syakur Achmad Poernomo Achmad Poernomo Achmad Poernomo Amiroh Amiroh Amiroh Amiroh, Amiroh Arimah Arimah Ayu Intan Sari Aziz, Kholifatul Azura Ulfa, Azura Budi Nurtama C. C. Nurwitri Cahyadi, Muhammad Cynthia Cynthia Cynthia CynthiaCynthia David Yudianto Dedi Fardiaz Ekawati Purwijantiningsih Ekawati Purwijantiningsih, Ekawati Eko Hari Purnomo Endang Tri Rahayu, Endang Tri Ermi Sukasih Estuningsih - Estuningsih - Fadiyah Hanifaturahmah Fenny Larasati Firman Fajar Perdhana Gathot Winarso, Gathot Gino Nemesio Cepeda Harsi D. Kusumaningrum Hesty Nur Fadia Istiana (alm.) Istiana Istiana, Istiana (alm.) Joko Riyanto Karina Nola Sinamo Komala, Kemal Lendrawati Lendrawati Lilis Nuraida Maggy T. Suhartono Maggy Thenawidjaja Suhartono Mala Nurilmala Muhammad Nur Mutaqin, Muhammad Zakki Nandika, Muhammad Rizki Nanin Anggraini, Nanin nFN Setyadjit Ni Gusti Ayu Made Widyatari Asthiti Ni Made Vina Citanirmala Nugroho Indrotristanto Nur Richana Nur Wulandari Nur Wulandari Nuri Andarwulan Nurwitri, C. C. Oryssa Sathalica Pradianti Pawestri, Wari Prasetio, Wiji Pratiwi Yuniarti Martoyo Pratiwi Yuniarti Martoyo Purwiyatno Hariyadi Rahadina Praba Melati Rahmawati Rahmawati Raini Panjaitan Ramadhani Meutia, Yuliasri Ratu Ayu Dewi Sartika Rika Puspitasari MZ Rinto . Sari, Ratna Nurmalita SEDARNAWATI YASNI Shanti Emawati Siti Nurjanah Siti Nurjanah Soegiarto, Salma Rachmanda Sri Estuningsih Suardana, A. A. Md. Ananda Putra Suci Apsari Pebrianti Sudiyono Sudiyono Sukarno Sukarno Supar, Supar Sutrisno Hadi Purnomo Tri Isti Rahayu Ulfah Amalia Uswatun Hasanah Vanessa Len Cahya Agustine Wati, Ari Kusuma Wijaya, Agung Dwi Wilis, Nabila Sekar Winiati P Rahayu Winiati P Rahayu Winiati P. Rahayu Winiati Pudji Rahayu Wiweka, Toh Jaya Yati Maryati Yati Maryati Yesica M. R. Sinaga Yesica Marcelina Romauli Sinaga Yuli Yanti Yuliasri Ramadhani Meutia Yusma Yennie Yusma Yennie

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