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Journal : Jurnal Natural

The role of Pediococcus pentosaceus IL13 in the fermentation of tuna waste, Euthynnus affinis RAMADHAN, ZULFA AULIA; SUNARTI, TITI CANDRA; MERYANDINI, ANJA
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.43091

Abstract

Processing tuna fish (Euthynnus affinis) produces wastes like heads, tails, and viscera, which can pollute the environment. However, this waste is nutrient-rich and can serve as a source of peptones, antimicrobials, and antioxidants. Align with Sustainable Development Goals (SDGs) through the zero-waste concept, which emphasizes reducing waste production, this study aims to analyze the role of Pediococcus pentosaceusIL13 in the fermentation of tuna fish waste. The fermentation process was conducted over 30 days using two types of fermentation, spontaneously and with the addition of P. pentosaceus IL13 culture. Bacterial dynamics, pH, protein content, and amino acid concentration were monitored to assess fermentation performance. The study found a reduction in total bacteria and lactic acid bacteria (LAB) along with an increase in pH in both types of fermentation over time. Maximum protein and free amino acid levels were observed on day 10. In the spontaneous fermentation, protein concentrations were 5.590.15% in the liquid phase and 20.051.19% in the solid phase. In the fermentation with added culture, protein concentrations were 6.590.57% in the liquid phase and 19.560.00% in the solid phase. Amino acid concentrations were 4821.11149.3 ppm for spontaneous fermentation and 6271.11377.1 ppm for fermentation with culture. The protein hydrolysates in the liquid phase have antibacterial activity against S. typhi.
In silico characterization of adh1 gene encoding alcohol dehydrogenase 1 (ADH1) from non-conventional yeast, Wickerhamomyces and Pichia spp HARTONO, FAISAL DINIAMAL; MERYANDINI, ANJA; ASTUTI, RIKA INDRI
Jurnal Natural Volume 25 Number 3, October 2025
Publisher : Universitas Syiah Kuala

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Abstract

Wickerhamomyces anomalus and Pichia kudriavzevii have high potential to produce bioethanol under high stress condition, due to their stress-tolerant properties. To elucidate and develop an efficient and sustainable bioethanol production, characterization of ethanol fermentation reactions is highly substantial. Ethanol fermentation employs key enzyme ADH1 encoded by ADH1 gene, important for conversion of acetaldehyde to ethanol. However, structural studies about alcohol dehydrogenase1 from these genera of yeasts are limited. This study aimed to detect the alcohol dehydrogenase 1 gene from Pichia spp. Using computational-bioinformatics approaches. The adh1 gene was amplified by PCR, visualized by electrophoresis, and analysed for sequence homology by BlastN and BlastP. The enzyme structure was constructed by SWISS-MODEL and I-TASSER with validation by Ramachandran plot, QMEAN4, and Local Quality Estimate. The Similarity and homology analysis of ADH1 genes and their corresponding protein sequence of yeast isolates showed that the ADH1gene was successfully detected. Multiple sequence alignment (MSA) and phylogenetic tree revealed that W. anomalus BT1-BT6 has close evolutionary relationship with ADH1 from Saccharomyces cerevisiae sequence while P. kudriavzevii IP4 showed different pattern. The ADH 1 enzyme model, generated using the SWISS-MODEL web server, demonstrated the best stereochemical quality, with a Ramachandran plot value of 100% for W. anomalus BT1 and 99.3% for P. kudriavzevii IP4. Superimposition of 3D-predicted model of ADH1 from W. anomalus BT1 and P. kudriavzevii 1P4 showed an exact match with amino acid in Zn2+ binding sites, confirming the ADH1 metaloenzyme properties. These findings provide structural insights about ADH1 genes and protein properties which can be used further for the development of efficient and high productivity of bioethanol productions through genetic and protein engineering.
Co-Authors . Hamim . KURRATAA’YUN . YOPI . YOPI ., Yopi ., Yopi A, Gading Wilda A, Gading Wilda Abd. Rasyid Syamsuri Abdjad Asih Nawangsih Adawiah, Adilah Ade Andriani ADE ANDRIANI Ade Irma Ahmad Thontowi Aisyah, Nadira Alfred Michael ALINA AKHDIYA Amor Tresna Karyawati, Amor Tresna Andika Susantri Anggreandari, Rizky Antonius Suwanto Apon Zaenal Mustopa Ardana Kurniaji Ariandi Arina Amalia Putri Aris Tri Wahyudi Ariyanto, Yogy Satria Armita, Dea Asrianti Basri Atit Kanti Azizah Hikma Safitri Azizah, Malikah Bambang Prasetya Basri, Asrianti Basri, Asrianti Bedah Rupaedah Berutu, Cocok Ana Maryani Berutu, Cocok Ana Maryani Besty Maranatha Birahy, Deford Cristy Candra Sunarti , Titi Cocok Ana Maryani Berutu Cynthia Rizka Riani DAROJATUL ULYA Deden Saprudin DERI YURATMOKO DEWI APRI ASTUTI Dewi, Fitria DIMAS ANDRIANTO DINI NURDIANI Dwi Ambarawati Dyah Iswantini EKA RURIANI Elly Rosyidah Engelhaupt, Martin Esti Utarti Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fathiah, Muhammad Fadhil Fathin Hamida Ferry Mutia Fitria Dewi Glisina Dwinoor Rembulan Hairani, Atikah Hamim Hamim Hamtini - Hamtini Hanni Tsaaqifah Hari Eko Irianto Hartono, Faisal Diniamal Hasrul Satria Hasrul Satria Nur Hasrul Satria, Hasrul I Komang Gede Wiryawan Ifah Munifah Iman Rusmana Inayah, Mazidah Noer IRA ERDIANDINI, IRA It Jamilah LAKSMI AMBARSARI Lenni Fitri Lenni Fitri Lilis Nuraida Lily Nathalia Loli Natalia Lukman, Zulfiqar Maggy T Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaya Suhartono Mahsunah, Anis Herliyati Manguntungi, Baso Maranatha, Besty Mareistia Fassah, Dilla Maria Sugiharti Marini Wijayanti Muhammad Nur Kholis, Muhammad Nur Muhammad Subhan Hamka MUNTI YUHANA MUTIA, FERY NANIK RAHMANI Nanik Rahmani Nanik Rahmani NAOMI, APRILIA Natalia, Loli Nathalia, Lily Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nunuk Widhyastuti NUNUK WIDHYASTUTI Nur Richana Nur, Hasrul Satria Nurfadhilla Rahmadhani Nurhasna, Aprilia Prihandono, Prima Agung Puspitasari, Dian Japany Rahayu Wulan RAMADHAN, ZULFA AULIA Rika Indri Astuti Rizky Anggreandari Rosyidah, Elly SAFITRI NURLAELA SHANTI RATNAKOMALA Sidiq, Muhammad Raffel Sipriyadi Sitti Rahbiah Akram SOFIYANTO, M. EDY Sri Estuningsih Sri Koerniati Sri Listiyowati SUHARSONO Sumarni Nompo Sunarti, Titi Suyono, Meisy Nawang Titi Candra Sunarti TRIO HENDARWIN Turnip, Enita Romasni Umi Fatmawati Vestika Iskawati Wahidul Hijah Wahyu Afrilasari Wahyu Widosari WIDANARNI WIDANARNI Widosari, Wahyu WULAN, RAHAYU Yantyati Widyastuti Yaya Rukayadi Yopi - Yopi Yopi YOPI YOPI Yopi, YULIANA, META YULIN LESTARI Zahrani, Siti Mutia