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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Soils BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Pascapanen Pertanian Indonesian Journal of Biotechnology Jurnal Sumberdaya Hayati BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology BIOEDUSCIENCE Jurnal Ilmu Nutrisi dan Teknologi Pakan ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Menara Perkebunan Makara Journal of Science Jurnal Natural Annales Bogorienses Berita Biologi
Anja Meryandini
Biology Department, Faculty Of Mathematics And Natural Sciences, IPB University And Biotechnology Center, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Veterinary

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Journal : JURNAL BIOLOGI INDONESIA

 1 
Hidrolisis Xilan Bagas Menggunakan Xilanase Bacillus subtilis XJ28 dan Karakterisasi Enzimnya A, Gading Wilda; ., Yopi; Meryandini, Anja
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2865.185 KB) | DOI: 10.14203/jbi.v11i1.2150

Abstract

Xylanase extracellular enzyme is produced by various microbes. Hydrolysis of bagasse xylan can produced xylooligosaccharide. The main components of hemicellulose was xylan at bagasse. IdentificationBacillus subtilisXJ28 using primer 16S RNA. The qualitative test used Congo-Red stainning, whereas quantitative test used Dinitrosalicyclic Acid (DNS) methode. The hydrolyzing product was analysed used TLC (Thin Layer Chromatography). This research aims were to characterized xylanase from isolate XJ28 and analyzing the hidrolyzing product from xylan bagasse. Pretreatment process included delignification using sodium hypochlorite 1% and xylans extraction using alkaline (NaOH 15%) as the solvent. The result of the xylans extracted from bagasse was 9,9% xylan and after purification was obtained 3,4% soluble xylan. Xylanase activity has the highest activity at 96 h of incubation time with activity 11,3 U/mL. Xylanase Bacillus subtilis XJ28has the optimum condition at pH 7 and 50 ºC and stable up to 72 hr of  incubation time at room temperature and 4 ºC. The hydrolysis product using xylanase crude enzyme was reducing sugar with molecular weight around  of sucrose and oligosaccharide. Keywords: xylanase, xylan bagasse, hydrolysis, TLC. 
Isolasi dan Seleksi Bacillus sp. dari Ikan Lele (Clarias sp.) serta Potensinya sebagai Probiotik ., Hamtini; ., Widanarni; Meryandini, Anja
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2778.371 KB) | DOI: 10.14203/jbi.v11i1.2151

Abstract

The aims of this study was to isolate and select Bacillus from the gut of catfish as probiotic candidates in the fish feed production. Isolation was conducted by heating samples at 80 °C for 10-15 minutes using Triptone Soy Agar (TSA) media which have been added with 1% skim milk for proteolytic activity and 1% starch for amylolytic activity. Selection was conducted based on pathogenicity test, antibiotic susceptibility test and total suspended solids. Isolate that have ability to degrade feed would be made the growth curves, analysis of protease and amilase activites and also combination of bacteria isolate with feed. Selected isolates as candidate probiotic were identified furthermore using 6S-rRNA gene. Among 16 isolates, there were 7 isolates that have gamma hemolytic activity (PTB 1.1, PTB 1.2, PTB 1.4, PTB 1.7, STB 1.6, STB 1.1 and STB 2.1). Antibiotic susceptibility test showed that 3 isolates were sensitive to the tested antibiotics (PTB 1.4, PTB 1.7 and STB 1.6). These three selected isolates were tested for their ability to degrade fish feed. PTB 1.4 isolate was able to degrade the feed with the smallest residue on the filter paper (0.0068 g). PTB 1.4 isolate also has proteolytic and amylolytic index of 0.61 and 0.60, respectively. Amylase activity of PTB 1.4 isolate added with 1.2% feed reached the highest peak in 120-hour of observation time (0.399 µ/mL) and the highest protease activity was in 72-hour of observation time (6.595  µ/mL). PTB 1.4 isolate has the ability to degrade the feed with the amount of 106 CFU/mL inoculum. Based on 16S-rRNA gene sequences isolate PTB 1.4 was 99% homolog with Bacillus megaterium. Isolation and selection of probiotic candidate from Clarias sp. get PTB 1.4 was a best isolate that there were not pathogenic, sensitive to antibiotic test, had protease and amilase activities. PTB 1.4 isolate had capability to degrade the feed. Keywords: Bacillus, Clarias sp., probiotic, feed 
ISOLASI DAN SELEKSI BACILLUS SP. DARI IKAN LELE (CLARIAS SP.) SERTA POTENSINYA SEBAGAI PROBIOTIK ., Hamtini; ., Widanarni; Meryandini, Anja
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2151

Abstract

The aims of this study was to isolate and select Bacillus from the gut of catfish as probiotic candidates in the fish feed production. Isolation was conducted by heating samples at 80 °C for 10-15 minutes using Triptone Soy Agar (TSA) media which have been added with 1% skim milk for proteolytic activity and 1% starch for amylolytic activity. Selection was conducted based on pathogenicity test, antibiotic susceptibility test and total suspended solids. Isolate that have ability to degrade feed would be made the growth curves, analysis of protease and amilase activites and also combination of bacteria isolate with feed. Selected isolates as candidate probiotic were identified furthermore using 6S-rRNA gene. Among 16 isolates, there were 7 isolates that have gamma hemolytic activity (PTB 1.1, PTB 1.2, PTB 1.4, PTB 1.7, STB 1.6, STB 1.1 and STB 2.1). Antibiotic susceptibility test showed that 3 isolates were sensitive to the tested antibiotics (PTB 1.4, PTB 1.7 and STB 1.6). These three selected isolates were tested for their ability to degrade fish feed. PTB 1.4 isolate was able to degrade the feed with the smallest residue on the filter paper (0.0068 g). PTB 1.4 isolate also has proteolytic and amylolytic index of 0.61 and 0.60, respectively. Amylase activity of PTB 1.4 isolate added with 1.2% feed reached the highest peak in 120-hour of observation time (0.399 µ/mL) and the highest protease activity was in 72-hour of observation time (6.595  µ/mL). PTB 1.4 isolate has the ability to degrade the feed with the amount of 106 CFU/mL inoculum. Based on 16S-rRNA gene sequences isolate PTB 1.4 was 99% homolog with Bacillus megaterium. Isolation and selection of probiotic candidate from Clarias sp. get PTB 1.4 was a best isolate that there were not pathogenic, sensitive to antibiotic test, had protease and amilase activities. PTB 1.4 isolate had capability to degrade the feed. Keywords: Bacillus, Clarias sp., probiotic, feed 
HIDROLISIS XILAN BAGAS MENGGUNAKAN XILANASE BACILLUS SUBTILIS XJ28 DAN KARAKTERISASI ENZIMNYA A, Gading Wilda; ., Yopi; Meryandini, Anja
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2150

Abstract

Xylanase extracellular enzyme is produced by various microbes. Hydrolysis of bagasse xylan can produced xylooligosaccharide. The main components of hemicellulose was xylan at bagasse. IdentificationBacillus subtilisXJ28 using primer 16S RNA. The qualitative test used Congo-Red stainning, whereas quantitative test used Dinitrosalicyclic Acid (DNS) methode. The hydrolyzing product was analysed used TLC (Thin Layer Chromatography). This research aims were to characterized xylanase from isolate XJ28 and analyzing the hidrolyzing product from xylan bagasse. Pretreatment process included delignification using sodium hypochlorite 1% and xylans extraction using alkaline (NaOH 15%) as the solvent. The result of the xylans extracted from bagasse was 9,9% xylan and after purification was obtained 3,4% soluble xylan. Xylanase activity has the highest activity at 96 h of incubation time with activity 11,3 U/mL. Xylanase Bacillus subtilis XJ28has the optimum condition at pH 7 and 50 ºC and stable up to 72 hr of  incubation time at room temperature and 4 ºC. The hydrolysis product using xylanase crude enzyme was reducing sugar with molecular weight around  of sucrose and oligosaccharide. Keywords: xylanase, xylan bagasse, hydrolysis, TLC. 
Co-Authors . Hamim . KURRATAA’YUN . YOPI . YOPI ., Yopi ., Yopi A, Gading Wilda A, Gading Wilda Abd. Rasyid Syamsuri Abdjad Asih Nawangsih Adawiah, Adilah Ade Andriani ADE ANDRIANI Ade Irma Ahmad Thontowi Aisyah, Nadira Alfred Michael ALINA AKHDIYA Amor Tresna Karyawati, Amor Tresna Andika Susantri Anggreandari, Rizky Antonius Suwanto Apon Zaenal Mustopa Ardana Kurniaji Ariandi Arina Amalia Putri Aris Tri Wahyudi Ariyanto, Yogy Satria Armita, Dea Asrianti Basri Atit Kanti Azizah Hikma Safitri Azizah, Malikah Bambang Prasetya Basri, Asrianti Basri, Asrianti Bedah Rupaedah Berutu, Cocok Ana Maryani Berutu, Cocok Ana Maryani Besty Maranatha Birahy, Deford Cristy Candra Sunarti , Titi Cocok Ana Maryani Berutu Cynthia Rizka Riani DAROJATUL ULYA Deden Saprudin DERI YURATMOKO DEWI APRI ASTUTI Dewi, Fitria DIMAS ANDRIANTO DINI NURDIANI Dwi Ambarawati Dyah Iswantini EKA RURIANI Elly Rosyidah Engelhaupt, Martin Esti Utarti Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Fathiah, Muhammad Fadhil Fathin Hamida Ferry Mutia Fitria Dewi Glisina Dwinoor Rembulan Hairani, Atikah Hamim Hamim Hamtini - Hamtini Hanni Tsaaqifah Hari Eko Irianto Hartono, Faisal Diniamal Hasrul Satria Hasrul Satria Nur Hasrul Satria, Hasrul I Komang Gede Wiryawan Ifah Munifah Iman Rusmana Inayah, Mazidah Noer IRA ERDIANDINI, IRA It Jamilah LAKSMI AMBARSARI Lenni Fitri Lenni Fitri Lilis Nuraida Lily Nathalia Loli Natalia Lukman, Zulfiqar Maggy T Suhartono Maggy Thenawidjaja Suhartono Maggy Thenawidjaya Suhartono Mahsunah, Anis Herliyati Manguntungi, Baso Maranatha, Besty Mareistia Fassah, Dilla Maria Sugiharti Marini Wijayanti Muhammad Nur Kholis, Muhammad Nur Muhammad Subhan Hamka MUNTI YUHANA MUTIA, FERY NANIK RAHMANI Nanik Rahmani Nanik Rahmani NAOMI, APRILIA Natalia, Loli Nathalia, Lily Niken Financia Gusmawati NISA RACHMANIA MUBARIK Nunuk Widhyastuti NUNUK WIDHYASTUTI Nur Richana Nur, Hasrul Satria Nurfadhilla Rahmadhani Nurhasna, Aprilia Prihandono, Prima Agung Puspitasari, Dian Japany Rahayu Wulan RAMADHAN, ZULFA AULIA Rika Indri Astuti Rizky Anggreandari Rosyidah, Elly SAFITRI NURLAELA SHANTI RATNAKOMALA Sidiq, Muhammad Raffel Sipriyadi Sitti Rahbiah Akram SOFIYANTO, M. EDY Sri Estuningsih Sri Koerniati Sri Listiyowati SUHARSONO Sumarni Nompo Sunarti, Titi Suyono, Meisy Nawang Titi Candra Sunarti TRIO HENDARWIN Turnip, Enita Romasni Umi Fatmawati Vestika Iskawati Wahidul Hijah Wahyu Afrilasari Wahyu Widosari WIDANARNI WIDANARNI Widosari, Wahyu WULAN, RAHAYU Yantyati Widyastuti Yaya Rukayadi Yopi - Yopi Yopi YOPI YOPI Yopi, YULIANA, META YULIN LESTARI Zahrani, Siti Mutia