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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Teknologi Dan Industri Pangan Jurnal Lanskap Indonesia Jurnal Pengolahan Hasil Perikanan Indonesia Media Gizi dan Keluarga Jurnal Sain Veteriner Jurnal Veteriner Jurnal Ilmu Lingkungan Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Majalah Kedokteran Bandung Jurnal Keteknikan Pertanian Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Medical Journal of Indonesia Indonesian Journal of Agricultural Science Jurnal Penelitian Pascapanen Pertanian Indonesian Journal of Human Nutrition Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) agriTECH Warta IHP (Warta Industri Hasil Pertanian) INDONESIAN JOURNAL OF PHARMACY Indonesian Food Science and TechnologyJournal JURNAL PANGAN Agroteknika Jurnal Mutu Pangan : Indonesian Journal of Food Quality Canrea Journal: Food Technology, Nutritions, and Culinary Journal World Nutrition Journal Narra J Jurnal Info Sains : Informatika dan Sains Jurnal Kedokteran Hewan Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research)

MADE ASTAWAN

Department Of Food Science And Technology, Faculty Of Agricultural Engineering And Technology, IPB University, Bogor, 16680, Indonesia

Author-ID : 235630

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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KARAKTERISTIK KONSENTRAT PROTEIN TERIPANG PASIR (Holothuria scabra J.) DENGAN BAHAN PENGEKSTRAK ASETON Karnila, Rahman; Astawan, Made; Sukarno, Sukarno; Wresdiyati, Tutik
Jurnal Perikanan dan Kelautan Vol 16, No 02 (2011)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.16.02.%p

Sea cucumber (Holothuria scabra J.) is a fishery that has a nutrientcontent and high economic value. This study aims to know: (1) nutrient content(proximate) sea cucumber fresh meat, (2) the yield of protein concentratemanufacture of sea cucumbers, (3) nutrient content of sea cucumber proteinconcentrate, and (4) total amino acid content of protein concentrates of seacucumber.Penelitian in five stages of the experiment: (1) preparation of meat freshsea cucumbers, (2) analysis of nutrient content (proximate) sea cucumber freshmeat, (3) the analysis of protein concentrate yield of seacucumbers, (4) analysis ofnutrient content (proximate) protein concentrate sea cucumbers, and (5) analysisof total amino acid content of sea cucumber protein concentrate. The resultsshowed nutrient content (proximate) sand sea cucumber fresh meat that is 87.03%water (bb): 76.64% protein (bk); 4.16% fat (bk), 14.34% ash (bk), and 4 , 93%carbohydrate (bk). The average yield produced in the manufacture of seacucumber protein concentrate was 9.87%. While the nutrient content (proximate)sea cucumber protein concentrate that is 8.97% water, 66.07% protein, 0.89% fat;11.53% ash, and 12.54% carbohydrate. The highest amino acid content in seacucumber protein concentrate is a proline (5.17%) and glutamic acid (3.23%).

Potensi Rambut Jagung sebagai Minuman Fungsional Salsabila Salsabila; Nurheni Sri Palupi; Made Astawan
JURNAL PANGAN Vol. 30 No. 2 (2021): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33964/jp.v30i2.542

Tanaman jagung dapat dimanfaatkan mulai dari akar, daun sampai buahnya. Akar tanaman jagungsudah banyak dimanfaatkan sebagai obat, daun jagung dimanfaatkan sebagai pakan ternak, dan buah jagungdimanfaatkan untuk sayur, popcorn, tepung jagung dan lainnya. Umumnya masyarakat tidak menggunakanrambut jagung dan menganggapnya sebagai sampah, padahal dalam rambut jagung terkandung senyawabioaktif, seperti flavonoid, fenolik, alkaloid, glikosida dan beta sitosterol yang berguna bagi kesehatan.Pada era global ini, jumlah radikal bebas semakin meningkat, sehingga berbagai senyawa bioaktif sangatdibutuhkan, terutama yang bersifat antioksidan. Konsumsi antioksidan yang dapat diperoleh dari rambutjagung diharapkan dapat menurunkan stres oksidatif sehingga dapat mencegah munculnya berbagaipenyakit degeneratif. Artikel ini merangkum bukti terkait potensi rambut jagung sebagai minuman fungsional.Metode yang digunakan adalah systematic review pada berbagai artikel ilmiah. Hasil menunjukkan bahwarambut jagung memiliki potensi sebagai minuman fungsional dikarenakan mengandung senyawa bioaktif(fenolik) yang cukup tinggi. Pada rambut jagung segar mengandung total fenolik sebesar 23,58 μgGAE/g,dan aktivitas antioksidan sebesar 50–83,54 persen. Pada rambut jagung kering mengandung total fenoliksebesar 38,7–7288,64 μgGAE/g dan aktivitas antioksidan sebesar 25–91persen.

Uji Biologis Iodium dari Rumput Laut (Eucheuma cottonii L.) dan Pengaruhnya terhadap Kemampuan Belajar Tikus Percobaan Astawan, Made; Wresdiyati, Tutik; Komari, Komari; Lasmiati, Ni Nengah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2005): February 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.2837

Intelligent is one of human quality factors that can be influenced by nutrition. Iodine is one of trace element nutrition that necessary for intelligent level. The purpose of this research was to learn the effect of seaweed as iodine source on rats learning ability. The rats were divided into four groups based on the ratio: A (without potassium iodide/KI-seaweed flour (SF) 0%), B (with KI-SF 0%), C (without KI-SF 5%) and D (without KI-SF 10%). The ratio had no significant effect on rats growth, but significant on iodine retention, neuron cell number and rats learning ability. Iodine retention value had a positive correlation with the neuron cell number. The neuron cell number of group A, B, C and D were 72.83, 89.33, 93.92 and 100.83 cells per view area, respectively. The number of neuron cells gave a significant influence on rats learning ability. Total time needed by the rats in food retrieval test for the group A, B, C and D was 57.84, 38.17, 33.39 and 20.55 seconds, respectively. The more neuron cell number, the more learning ability.

The Effect of Seaweed Eucheuma cottonii on Superoxide Dismutase (SOD) Liver of Hypercholesterolemic Rats TUTIK WRESDIYATI; ANS BUDI HARTANTA; MADE ASTAWAN
HAYATI Journal of Biosciences Vol. 15 No. 3 (2008): September 2008
Publisher : Bogor Agricultural University, Indonesia

Intracellular antioxidant superoxide dismutase (SOD) was reported decreased in the liver and kidney of hypercholesterolemic rats. This study was conducted to observe the effect of seaweed Eucheuma cottonii powder on the profile of blood cholesterol and the level of SOD in liver tissues of hypercholesterolemic rats by using immunohistochemical technique. Twenty male Wistar rats were used for this study. Those rats were divided into four groups; (i) negative control group (A), (ii) hypercholesterolemia group treated by 5% seaweed powder (B), (iii) hypercholesterolemia group treated by 10% seaweed powder (C), and (iv) Positive control group or hypercholesterolemia group (D). The experiment was carried out for 35 days. Hypercholesterolemia condition (> 130 mg/dl), except group A, was achieved by feeding the rats with commercial diet containing 1% cholesterol. Drinking water was given ad libitum for 40 days. The results showed that seaweed powder decreased the total cholesterol, low density lipoprotein (LDL), triglyceride, and increased the level of high density lipoprotein (HDL) and SOD status in the liver tissues of hypercholesterolemic rats. The treatment of 10% seaweed powder gave better results than that of 5%. These results suggested that dietary fiber such in the seaweed powder has antioxidant activity. Key words: superoxide dismutase (SOD), seaweed-Eucheuma cottonii, hypercholesterolemia, liver, immunohistochemistry

The Utilization of Seaweed as a Source of Dietary Fiber to Decrease the Serum Cholesterol in Rats MADE ASTAWAN; TUTIK WRESDIYATI; ANZS BUDY HARTANTA
HAYATI Journal of Biosciences Vol. 12 No. 1 (2005): March 2005
Publisher : Bogor Agricultural University, Indonesia

The cholesterol lowering effect of seaweed (Eucheuma cottonii) powder as a source of dietary fiber was evaluated in hypercholesterolemic rats. Four groups of five male Sprague Dawley hypercholesterolemic rats were fed a 0% cholesterol-0% seaweed powder (negative control); 1% cholesterol-5% seaweed powder; 1% cholesterol-10% seaweed powder; and 1% cholesterol-0% seaweed powder (positive control) for 35 days. Seaweed powder contained feed did not affect the growth of rats but significantly lowered the serum total cholesterol, LDL cholesterol, triglyceride, and atherogenic index. The lowest serum cholesterol was found in the hypercholesterolemic rats fed with 1% cholesterol-10% seaweed powder. The values of total-cholesterol, LDL-cholesterol, and triglyceride were 67.7, 33.0, and 47.3 mg/dl, respectively.

The Immunohistochemical Profile of Superoxide Dismutase (SOD) in the Liver Tissue of Hypercholesterolemic Rats TUTIK WRESDIYATI; MADE ASTAWAN; LUSIA YUNI HASTANTI
HAYATI Journal of Biosciences Vol. 13 No. 3 (2006): September 2006
Publisher : Bogor Agricultural University, Indonesia

This study was conducted to observe intracellular antioxidant cooper,zinc-superoxide dismutase (Cu,Zn-SOD) in liver tissue of rats under hypercholesterolemic condition by using immunohistochemical technique. A total of twenty male Wistar rats were used for this study. Those rats were divided into two groups; (i) control group and (ii) hypercholesterolemic group, which were fed died containing 1% cholesterol for eight weeks. Rat livers were taken at the end of treatment, and processed by using paraffin embedding standard method. The tissues were stained immunohistochemically to Cu,Zn-SOD. Observation of Cu,Zn-SOD content in the tissue was performed qualitatively in the cytoplasm and quantitatively in the nucleus of hepatocytes based on colour intensity of enzyme reaction product. The profile of antioxidant-Cu,Zn-SOD decreased (P < 0.05) in the hypercholesterolemic group compared to the control group. Key words: Superoxide dismutase (SOD), hypercholesterolemia, liver, rat

Comparison Between the Potential of Tempe Flour Made from Germinated and Nongerminated Soybeans in Preventing Diabetes Mellitus Made Astawan; Inas Suci Rahmawati; Ananda Putri Cahyani; Tutik Wresdiyati; Sastia Prama Putri; Eiichiro Fukusaki
HAYATI Journal of Biosciences Vol. 27 No. 1 (2020): January 2020
Publisher : Bogor Agricultural University, Indonesia

This study was aimed to compare the chemical characteristics of tempe flour made from nongerminated soybean (NST) and germinated soybean (GST), especially on their capacity in preventing diabetes mellitus (DM). Soybeans were germinated for 20 hours in the dark until 2.5-5.0 mm of the radicle emerged. The ungerminated soybeans and the germinated soybeans were then processed into tempe and tempe flour. The two types of tempe flour were subjected to proximate analysis, amino acid profiling, antioxidant capacity, total phenol content, isoflavone content, and α-amylase and α-glucosidase enzyme inhibition analyses. GST was superior in preventing DM in the protein content and antioxidant parameters, as these were significantly higher (p<0.05) than in NST. On the other hand, NST was superior in preventing diabetes in the isoflavon (daidzein, genistein, and total isoflavone) and α-amylase inhibition IC50 parameters which were significantly better (p<0.05) than in GST. On the contrary, the diabetes-preventing parameters total phenols, α-glucosidase inhibition IC50, and insulinotropic amino acids (arginine, alanine, phenilalanine, isoleucine, leucine, and lysine) were not different (p>0.05). Therefore, GST and NST both have potential in preventing diabetes through different mechanisms.

Tempe and Soybean var. Grobogan-Indonesia Increased The Number of Osteoblasts and Osteocytes, Inhibited Osteoclast Damage in The Tibia Bone of Rats Tutik Wresdiyati; Alamsah Firdaus; Made Astawan
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.144

Tempe, an Indonesian traditional food, is a product of processed soybeans by fermentation. Both tempe and soybeans contain a variety of nutrients, such as isoflavone, Ca, P, and Mg that are important for bone health. This study aimed to observe the influence of Indonesian var. Grobogan tempe flour and soybean flour on the profile of osteoblasts, osteocytes, and osteoclasts in the tibia bone of rats. The rats were divided into five groups; casein, tempe flour 10%, tempe flour 20%, boiled soybean flour 10%, and boiled soybean flour 20% groups. The treatment was conducted for 90 days. The results showed that the treatment using tempe flour and soybean flour increased the number of osteoblasts and osteocytes significantly compared to the casein group. Tempe flour 20% showed the best result. In addition, the number of osteoclasts in the tibia bone of rats that were treated with tempe flour and soybean flour were smaller than that of negative control group. The treatment using soybean flour (10% and 20%) or tempe flour (10% and 20%) decreased the number of osteoclasts in the tibia bone of rats. It is suggested that both tempe and soybeans can be utilized for osteoporosis prevention.

EVALUASI KESEPADANAN MUTU GIZI TEMPE KEDELAI PANGAN REKAYASA GENETIK (PRG) DAN NON-PRG SERTA DAMPAK KONSUMSINYA PADA TIKUS PERCOBAAN Dadi Hidayat Maskar; Hardinsyah Hardinsyah; Evy Damayanthi; Made Astawan; Tutik Wresdiyati
Jurnal Gizi dan Pangan Vol. 10 No. 3 (2015)
Publisher : Food and Nutrition Society of Indonesia

ABSTRACTThis study was conducted to evaluate the effect of tempe that were made from Genetically Modified (GM) and non-GM soybean on protein quality, malondialdehide (MDA) levels, intracellular antioxidant superoxide dismutase (SOD) activity in the liver and kidneys, as well as spermatozoa profile of experimental rats. Fourty five Sprague Dawley rats divided into eight treatment grups and one control, fed with tempe and soybean from GM and non-GM at 10% and 20% concentrations for 90 days. The results showed that there was no significant difference in term of protein quality, tempe made from GM soybean is substantially equivalent with tempe made from non-GM soybean. Results showed that group which was given ration of 10% protein from conventional soybean had lower liver and kidney MDA levels as compared to GM tempe 10% and 20% groups, but was not significant compared to conventional soybean 20% and casein 10% groups. While the value of liver and kidney SOD activity were not significantly different (p>0.05) between the groups of rats. There was no significant differences among the spermatozoa profiles treatment groups and control and they were within normal condition. Results of protein quality, MDA, SOD, and spermatozoa profile showed that tempe made from GM soybean was substantially equivalent with the non-GM soybean.Keywords: GM soybean, MDA, non-GM tempe, SOD, spermatozoa profileABSTRAKTujuan penelitian ini adalah untuk mengevaluasi pengaruh tempe Pangan Rekayasa Genetik (PRG) dan non-PRG meliputi kualitas protein, kadar malondialdehida (MDA) hati dan ginjal, aktivitas superoksida dismutase (SOD) hati dan ginjal, dan profil spermatozoa pada tikus percobaan. Sebanyak 45 tikus terbagi ke dalam delapan perlakuan dan satu kontrol yang diberikan perlakuan dengan ransum tempe dan kedelai, baik PRG maupun non-PRG, dengan konsentrasi 10% dan 20% selama 90 hari. Hasil percobaan menunjukkan bahwa tidak ada perbedaan yang signifikan dalam kualitas protein, tempe PRG memiliki nilai yang sama dengan tempe non-PRG. Tikus yang diberi ransum 10% protein kedelai non-PRG mempunyai nilai MDA hati dan ginjal yang lebih rendah dibandingkan dengan tempe PRG 10% dan 20%, tetapi tidak signifikan dengan kelompok kedelai non-PRG 20% dan kasein 10%. Sementara itu, nilai SOD hati dan ginjal tidak signifikan antar grup perlakuan (p>0,05). Tidak ada perbedaan yang signifikan pada profil spermatozoa antar perlakuan. Hasil dari kualitas protein, MDA, SOD, dan profil spermatozoa menunjukkan bahwa kedelai dan tempe PRG memiliki kesamaan substansial dengan kedelai dan tempe non-PRG.Kata kunci: MDA, profil spermatozoa, SOD, tempe kedelai PRG, tempe kedelai non-PRG

EFEK INTERVENSI MINUMAN TEMPE TERHADAP PENURUNAN KADAR LOW DENSITY LIPOPROTEIN Ika Wirya Wirawanti; Hardinsyah Hardinsyah; Dodik Briawan; Made Astawan
Jurnal Gizi dan Pangan Vol. 12 No. 1 (2017)
Publisher : Food and Nutrition Society of Indonesia

The aim of this study was to analyze the effect of tempe drink intervention on lipid profile of hypercholesterolemic subjects. This study used a Randomized Controlled Trial (RCT) design with 51 males and females subjects with the inclusion criterias were adults aged 25-55 years, not being menopause and pregnant, total cholesterol levels ≥200 mg/dl and signed informed consent. The subjects were divided into three treatments - Tempe Drink of A (TDA) formulated from local sprouted soybean, Tempe Drink of B (TDB) formulated from imported soybean and control. The tempe drink was given three glasses a day for four weeks, contained at least 25 g of protein/day. The control treatment was not given the tempe drink. Lipid profile (total cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides (TG)) was collected before and after intervention. The results showed that both TDA and TDB decreased total cholesterol, LDL, and TG of subjects, respectively 26.7 mg/dl and 17.3 mg/dl; 3.6 mg/dl and 1.0 mg/dl; and 16.2 mg/dl and 3.7 mg/dl compared to control subjects, but did not increase HDL of subjects. This implies that tempe drink had beneficial health effect on lowering total cholesterol, LDL and TG.

Co-Authors A.A. Ketut Agung Cahyawan W Adurrasyid, Zaid Afifah, Diana N. Afrilia Sandra Ramadhani Agata Tantri Atmaja Ahmad Sulaeman Ahmad Syauqy Alamsah Firdaus Alfia Ansarullah Ali, Muhammad Saddam Amilia Dayatri Uray Anak Agung Istri Sri Wiadnyani Ananda Putri Cahyani Ananda Putri Cahyani Andi Early Febrinda Ani Karmila Anjani, Gemala Ans Budi Hartanta ANZS BUDY HARTANTA Arif - Hartoyo Armando M Saragih Ati Widya Perana Ayu P.G Prayudani Azka Lathifah Zahratu Azra B.A. Susila Santosa Bambang Purwantara Bella Dinar Fauqii Cahyani Bernadetha Beatrix Sibarani C Hanny Wijaya Cahyani, Ananda Putri Chitisankul, Wanida T. Dadang Supriatna Dadang Supriatna Dadang Supriatna Dadi Hidayat Maskar Dahrulsyah - - Damayanti, Aprilia F. Deddy - Muchtadi DEDDY MUCHTADI Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Diana Nur Afifah, Diana Nur Diini Fithriani Dini Wulan Dari Dodik Briawan Dwi Febiyanti - Dwi Utami, Septi Eiichiro Fukusaki Elis Nurhayati Endang Mahati Endang Prangdimurti Evy Damayanthi Fanie Herdiani Fithriani, Diini Fransiska R Zakaria Hadi Riyadi Hadi Susilo Arifin Hadiningtias, Primanisa Hamzah Alfarisi Hardinsyah Hazmi, Khaidar Herpandi . Hidayati, Mustika I Komang Gede Wiryawan Ichsani, Nadya Ika Wirya Wirawanti Ikeu Tanziha Inas Suci Rahmawati Indira Saputra Intan Kusumawati Irma Isnafia Arief Isnafia Arief , Irma Jefriaman Sirait Karnila, Rahman Karnila Ketut Adnyane Mudite Khaidar Hazmi Komang G Wiryawan Komari Komari Komari Komari Lasmiati, Ni Nengah Laut, Bimaris Tranoya Leonita Maulidyanti LUSIA YUNI HASTANTI Manalu, Johanes Marojahan Mardhiyyah, Yunita Siti Maryani Suwarno Maryani Suwarno Maryani Suwarno Maulidyanti, Leonita Muchtadi, Deddy Muhamad Firdaus Muhamad Firdaus Muhamad Syukur Muhammad Agus Muljanto Muhammad Aries Muhammad Ichsan Mursyid . Mursyid Mursyid Mustika Hidayati Nadya Ichsani Nafisah Nancy Dewi Yuliana nFN Akhyar Ni Nengah Lasmiati Novita, Rias R. Nur Wulandari Nurani Istiqomah Nurhayati Arifin Nurhayati H.S. Arifin Nurhayati Nurhayati Nurhayati, Elis Nurina Rachma Adiningsih Palupi, Nurheni Sri Perana, Ati Widya Prasetyawati, Renny Candra Prayudani, Ayu P G Prayudani, Ayu Putri Gitanjali Prima Yaumil Fajri Putri, Sastia P. Putri, Sastia Prama Putty Anggi Lestari Rachma Adiningsih, Nurina Rafidha Irdiani Rahmawati, Irma Sarita Rahmawati, Siti Irma Ramadhani, Afrilia Sandra Ramdhani, Rizal Pauzan Ratnaningsih Eko S. Renny Candra Prasetyawati Rimbawan , Rini Kesenja Rita Khairina RR. Ella Evrita Hestiandari Rudy R Nitibaskara Saithong, Pramuan Salsabila Salsabila Sam Herodian Sandra Arifin Aziz Saputra, Indira Saragih, Armando M SARASWATI SARASWATI Sarwono Waspadji Sarwono Waspadji Sarwono Waspadji Sastia Prama Putri Septi Dwi Utami Setyawati S Karyono Setyawati S. K. Setyawati S. Karyono Setyawati, Amalia Rani Sibarani, Bernadetha Beatrix Siti Harnina Bintari Siti Sa'diah Siti Sadiah Slamet Widodo Soewarno S Soekarto Soewarno Soekarto Sri Anna Marliyati Sri Rahmatul Laila Sri Widowati Sri Widowati Sugeng Heri Suseno Sukarno Sukarno Suliantari . Sundari, Fitria Suci Suratno, Yuhlanny Dewi Sussi Astuti Sutrisno Koswara Taopik Ridwan TATI NURHAYATI Tika Pratiwi Khumairoh Tita Aviana Tjahja Muhandri Tryas, Anisha Ayuning Tuti Wresdiyati Tutik Wresdiyati Utami, Sri Inten V Prihananto Vera Di Nurwati Winiati P. Rahayu Winiati Pudji Rahayu Wresdiyati, Tuti Yana Nurdiana Yeni Setiorini Yeni Setiorini Yenni MS Nababan Yuhlanny Dewi Suratno Yuspihana Fitrial

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