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Study of Fermented Food Intervention on the Serum Lipid Profile of Hypercholesterolemia Animals Trial (Meta-analysis) Tryas, Anisha Ayuning; Astawan, Made; Saraswati, Saraswati
Indonesian Food Science and Technology Journal Vol. 8 No. 2 (2025): Volume 8. Number 2, July 2025 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v8i2.36353

Abstract

Abstract— Individuals with hypercholesterolemia (HC) require specific therapy, such as statin medication, to maintain their blood lipid profile (total cholesterol, triglycerides, HDL-C, LDL-C) within the normal range. In vivo, studies have shown that consuming fermented foods for a particular duration can help improve the blood lipid profile of HC animal models. However, meta-analysis studies have not investigated which type of fermented food has the most significant effect on improving the blood lipid profile. The studies included were in vivo research that examined the effects of fermented food interventions on improving the blood lipid profile in HC animal models. The results of this study indicate that fermented food interventions (both plant-based and animal-based) have a highly significant effect (p-value <0.001) on improving the blood lipid profile compared to the HC animal model group without intervention. However, the plant-based fermented food source group tends to show a more significant effect on lipid profiles than those sourced from animals.
INSTANT GRANULES OF Zingiber officinale AND Cajanus cajan LEAVES IMPROVED LIVER HISTOLOGICAL PROFILE IN DIABETIC MODEL RATS Manalu, Johanes Marojahan; Sadiah, Siti; Aziz, Sandra Arifin; Astawan, Made; Ridwan, Taopik; Wresdiyati, Tutik
Jurnal Kedokteran Hewan Vol 19, No 3 (2025): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i3.47330

Abstract

Diabetes mellitus can cause significant microstructural damage in liver tissue. This study evaluated the effectiveness of instant granules of Zingiber officinale and Cajanus cajan leaves on the liver histological profile in diabetic model rats. A total of 25 male Sprague Dawley rats were assigned into five groups. The group consist of negative control (NC), positive control (PC), metformin control group at a dose of 150 mg/kg BW (MET), and two treatment groups of instant granules of Z. officinale and C. cajan leaves at a dose of 300 mg/kg BW (T1) and 150 mg/kg BW (T2) for 28 days treatment. The diabetic condition was obtained by single induction of streptozotocin (35 mg/kg BW). The liver tissue was stained using hematoxylin-eosin staining to observe the cytoplasm intensity, central vein diameter, and nucleus intensity of liver cells in diabetic model rats. The results showed that administration of the instant granules significantly decrease liver microstructural damage (P0.01). The 150 mg/kg BW (T2) dose showed the most optimal effectiveness by successfully decreasing cytoplasmic intensity, reducing central vein dilation, and decreasing nuclear cell intensity. The research result indicate that instant granules have strong hepatoprotective properties that can repair liver microstructural damage caused by diabetes.
PHYSICO-CHEMICAL VALUE AND HYPOGLYCEMIC EFFECT OF INDUSTRIAL-GRADE ETHANOLIC EXTRACT OF PIGEON PEA (Cajanus cajan) LEAVES AND GINGER (Zingiber officinale var. amarum) Wresdiyati, Tutik; Alfarisi, Hamzah; Putri, Anisya Saeila; Abidah, Puri Adzrok; Darawati, Made; Aziz, Sandra; Sadiah, Siti; Astawan, Made; Santoso, Koekoeh
Jurnal Kedokteran Hewan Vol 18, No 1 (2024): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i1.31691

Abstract

This study aimed to analyze flavonoid and 6-gingerol content and to test the hypoglycemic effects of industrial-grade Cajanus cajan (pigeon pea) leaf and Zingiber officinale rhizome (ginger) extracts on hyperglycemic rats. Extraction utilized maceration with 70% alcohol at PT. Insular Multi Natural. Extracts were characterized and analyzed for flavonoids and 6-gingerol content. Hypoglycemic activity was assessed using the oral glucose tolerance test (OGTT) on rats with induced hyperglycemia via 90% sucrose solution. The results indicated higher flavonoid content in C. cajan leaf extract (4090.09338.23 mg/100 g) compared to Z. officinale extract (622.74203.39 mg/100 g), while gingerol content in Z. officinale extract was 701.524.42 mg/100 g. This study concluded that combination of industrial-grade pigeon pea leaves extract and ginger extract reduce the level of blood glucose in hyperglycemic model rats at all combination dose (150:150, 200:100, and 100:200 mg/kg BW). The combination dose of 200:100 mg/kg BW showed the best blood glucose level profile.
PROBIOTIK LOKAL MENINGKATKAN KANDUNGAN IgA USUS HALUS TIKUS YANG DIINFEKSI ENTEROPATHOGENIC E.Coli (EPEC): STUDI IMUNOHISTOKIMIA Wresdiyati, Tutik; Setiorini, Yeni; Laila, Sri Rahmatul; Arief, Irma Isnafia; Astawan, Made
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.921

Abstract

Penelitian ini bertujuan menganalisis pengaruh pemberian probiotik lokal Lactobacillus fermentum (L. fermentum) dan Lactobacillus plantarum (L. plantarum) terhadap profil kandungan IgA usus halus tikus yang diinfeksi enteropatogenik E. coli (EPEC) menggunakan teknik imunohistokimia. Sebanyak 90 ekor tikus jantan galur Sprague Dawley digunakan dan dibagi menjadi enam kelompok perlakuan, yaknikelompok kontrol negatif (A), kelompok perlakuan L. plantarum (B), kelompok perlakuan L. fermentum (C), kelompok perlakuan L. plantarumdan EPEC (D), kelompok perlakuan L. fermentum dan EPEC (E), dan kelompok perlakuan EPEC (F). Perlakuan dilaksanakan selama 21 hari. Deteksi IgA dilakukan dengan teknik imunohistokimia pada jaringan usus halus. Hasil penelitian menunjukkan bahwa perlakuan probiotik lokalL. fermentum selama 2-3 minggu, dan perlakuan L. plantarum selama 2 minggu mampu meningkatkan kandungan IgA di usus halus tikus. Pada tikus yang dipapar EPEC, L. fermentum lebih baik dalam meningkatkan kandungan IgA dibandingkan L. plantarum.
Analisis Karakteristik Pekarangan dalam Mendukung Penganekaragaman Pangan Keluarga di Kabupaten Bogor Azra, Azka Lathifah Zahratu; Arifin, Hadi Susilo; Astawan, Made; Arifin, Nurhayati HS
Jurnal Lanskap Indonesia Vol. 6 No. 2 (2014): Jurnal Lanskap Indonesia
Publisher : http://arl-faperta.ipb.ac.id/

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jli.v6i2.16552

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Pekarangan is a type of traditional Indonesian home gardens that utilize the land around the house with the status and clear boundaries. Pekarangan in rural areas have high agrobiodiversity, good agroecosystem and should be optimized as an area to meet the needs of daily life, especially to support the diversification of food consumption of the household. Therefore, the purpose of this study is to analyze the ecology characteristics of pekarangan to support food consumption diversification of the household. The study was conducted in Bogor Regency, which located in altitudes at 165 – 460 m height with high level of urbanization, from December 2013 to June 2014. Diversity of food in pekarangan, specially the crops and livestocks, is the focus of this research. The results of the study showed that the most common crops in Bogor Regency are vegetables, fruits, and spices plants. Analysis of plant diversity index showed that the pekarangan in Bogor Regency has a diversity in the mid category (1.95) with the dominance of seasonal crops. Diversification of food in pekarangan can be done by optimizing the existing area, utilization of all zoning for the cultivation of diverse functions crops and livestock. For diversification of food consumption, the owner should use different types of food, as well as considering the crop calendar to a wide variety of food that can be consumed on a daily basis. Therefore, it's necessary to educate the housewives about the potency of diversification crops in pekarangan for consumption, strong motivation and assistance from the government, as well as optimizing the role of KWT group to optimize the role of pekarangan for the diversification of food.
EVALUASI KESEPADANAN MUTU GIZI TEMPE KEDELAI PANGAN REKAYASA GENETIK (PRG) DAN NON-PRG SERTA DAMPAK KONSUMSINYA PADA TIKUS PERCOBAAN Maskar, Dadi Hidayat; Hardinsyah, Hardinsyah; Damayanthi, Evy; Astawan, Made; Wresdiyati, Tutik
Jurnal Gizi dan Pangan Vol. 10 No. 3 (2015)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.768 KB) | DOI: 10.25182/jgp.2015.10.3.%p

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ABSTRACTThis study was conducted to evaluate the effect of tempe that were made from Genetically Modified (GM) and non-GM soybean on protein quality, malondialdehide (MDA) levels, intracellular antioxidant superoxide dismutase (SOD) activity in the liver and kidneys, as well as spermatozoa profile of experimental rats. Fourty five Sprague Dawley rats divided into eight treatment grups and one control, fed with tempe and soybean from GM and non-GM at 10% and 20% concentrations for 90 days. The results showed that there was no significant difference in term of protein quality, tempe made from GM soybean is substantially equivalent with tempe made from non-GM soybean. Results showed that group which was given ration of 10% protein from conventional soybean had lower liver and kidney MDA levels as compared to GM tempe 10% and 20% groups, but was not significant compared to conventional soybean 20% and casein 10% groups. While the value of liver and kidney SOD activity were not significantly different (p>0.05) between the groups of rats. There was no significant differences among the spermatozoa profiles treatment groups and control and they were within normal condition. Results of protein quality, MDA, SOD, and spermatozoa profile showed that tempe made from GM soybean was substantially equivalent with the non-GM soybean.Keywords: GM soybean, MDA, non-GM tempe, SOD, spermatozoa profileABSTRAKTujuan penelitian ini adalah untuk mengevaluasi pengaruh tempe Pangan Rekayasa Genetik (PRG) dan non-PRG meliputi kualitas protein, kadar malondialdehida (MDA) hati dan ginjal, aktivitas superoksida dismutase (SOD) hati dan ginjal, dan profil spermatozoa pada tikus percobaan. Sebanyak 45 tikus terbagi ke dalam delapan perlakuan dan satu kontrol yang diberikan perlakuan dengan ransum tempe dan kedelai, baik PRG maupun non-PRG, dengan konsentrasi 10% dan 20% selama 90 hari. Hasil percobaan menunjukkan bahwa tidak ada perbedaan yang signifikan dalam kualitas protein, tempe PRG memiliki nilai yang sama dengan tempe non-PRG. Tikus yang diberi ransum 10% protein kedelai non-PRG mempunyai nilai MDA hati dan ginjal yang lebih rendah dibandingkan dengan tempe PRG 10% dan 20%, tetapi tidak signifikan dengan kelompok kedelai non-PRG 20% dan kasein 10%. Sementara itu, nilai SOD hati dan ginjal tidak signifikan antar grup perlakuan (p>0,05). Tidak ada perbedaan yang signifikan pada profil spermatozoa antar perlakuan. Hasil dari kualitas protein, MDA, SOD, dan profil spermatozoa menunjukkan bahwa kedelai dan tempe PRG memiliki kesamaan substansial dengan kedelai dan tempe non-PRG.Kata kunci: MDA, profil spermatozoa, SOD, tempe kedelai PRG, tempe kedelai non-PRG
EFEK INTERVENSI MINUMAN TEMPE TERHADAP PENURUNAN KADAR LOW DENSITY LIPOPROTEIN Wirawanti, Ika Wirya; Hardinsyah, Hardinsyah; Briawan, Dodik; Astawan, Made
Jurnal Gizi dan Pangan Vol. 12 No. 1 (2017)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.641 KB) | DOI: 10.25182/jgp.2017.12.1.9-16

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The aim of this study was to analyze the effect of tempe drink intervention on lipid profile of hypercholesterolemic subjects. This study used a Randomized Controlled Trial (RCT) design with 51 males and females subjects with the inclusion criterias were adults aged 25-55 years, not being menopause and pregnant, total cholesterol levels ≥200 mg/dl and signed informed consent. The subjects were divided into three treatments - Tempe Drink of A (TDA) formulated from local sprouted soybean, Tempe Drink of B (TDB) formulated from imported soybean and control. The tempe drink was given three glasses a day for four weeks, contained at least 25 g of protein/day. The control treatment was not given the tempe drink. Lipid profile (total cholesterol, low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides (TG)) was collected before and after  intervention. The results showed that both TDA and TDB decreased total cholesterol, LDL, and TG of subjects, respectively 26.7 mg/dl and 17.3 mg/dl; 3.6 mg/dl and 1.0 mg/dl; and 16.2 mg/dl and 3.7 mg/dl compared to control subjects, but did not increase HDL of subjects. This implies that tempe drink had beneficial health effect on lowering total cholesterol, LDL and TG.
Efek intervensi minuman tempe terhadap tekanan darah penderita hipertensi dan hiperkolesterolemia Ansarullah, Alfia; hardinsyah, Hardinsyah; Marliyati, Sri Anna; Astawan, Made
Jurnal Gizi dan Pangan Vol. 12 No. 2 (2017)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.65 KB) | DOI: 10.25182/jgp.2017.12.2.101-108

Abstract

The aim of this study was to determine the effect of tempe drink intervention on blood pressure of hypertension and also hypercholesterolemia subjects. This study used a Randomized Controlled Trial (RCT) design with 30 males and females subjects, who were divided into three treatment groups-tempe drink of A formulated from local sprouted soybean (TDA), tempe drink of B formulated from imported soybean (TDB), and control. The inclusion criterias were adults aged 25-55 years, not being menopause and pregnant, total cholesterol levels ≥200 mg/dl, systolic blood pressure level 121-139 mmHg, diastolic blood pressure level 81-89 mmHg and willing to partcipate in research by signing an informed consent. The tempe drink was given three glasses a day for four weeks continuously, contained at least 25 g of protein/day. The control group was not given the tempe drink. Blood pressure data were collected every week during intervention. The results showed that the effect of TDA and TDB compared to control subjects on systolic blood pressure was significantly different. Other ways the effect of TDA and TDB compared to control subjects on diastolic blood pressure was not significantly different, but the trend was decreasing.
Potential of Bioactive Components in Tempe for the Treatment of Obesity Astawan, Made; Mardhiyyah, Yunita Siti; Wijaya, Christofora Hanny
Jurnal Gizi dan Pangan Vol. 13 No. 2 (2018)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.19 KB) | DOI: 10.25182/jgp.2018.13.2.79-86

Abstract

Obesity has become a global health issue and is one of the factors that trigger degenerative diseases. The correct food consumption management could be a solution for treating obesity. Soybean is a food that is rich in bioactive components and has antiobesity properties through various mechanisms. In Indonesia, nearly 60% of the soybeans are consumed in the form of tempe. The process of fermenting soybeans into tempe causes a bioconversion of nutrients and bioactive components, improving the active physiological abilities. The bioactive components that play a role in the treatment of obesity are isoflavones, proteins, and peptides. These bioactive components help in reducing body weight, lowering the body fat ratio and improve lipid profile. Thus, optimation and popularization of tempe as a functional food in the daily menu supported with correct tempe processing could be a solution in treating obesity.
Assessment of Sodium Content of Processed Food Available in Indonesia Istiqomah, Nurani; Astawan, Made; Palupi, Nurheni Sri
Jurnal Gizi dan Pangan Vol. 16 No. 3 (2021)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.349 KB) | DOI: 10.25182/jgp.2021.16.3.129-138

Abstract

This study was conducted to identify the sodium content in processed food and determine the proportion of products that meet the World Health Organization (WHO) global sodium benchmark criteria. Spesific concern was placed for sodium content in instant noodles. A comparative analysis was conducted to determine the relevance between the WHO and Indonesian Food and Drug Authority (Indonesian FDA) benchmarks in limiting sodium in instant noodles. Data on sodium levels (mg/100 g) of processed food was obtained from the register in the Indonesian FDA, Directorate of Processed Food Registration for 2019 to 2020. There were 3,850 products, consisting of 3,036 Local Products (LP) and 814 Imported Products (IP). These products were grouped into seven food categories and 18 types of food. The highest sodium content was found in chili sauce at 2,254,06 mg/100 g, and the lowest was in wafers at 218.64 mg/100 g. Overall, 2,538 of all products (66.56%) did not meet the sodium criteria based on the WHO benchmark. While for instant noodles, only 14.2% of the products met the sodium criteria based on the Indonesian FDA regulations and only 7.4% comply to the WHO sodium benchmark. The Cohen’s Kappa test showed a strong agreement (K=0.650; 95% CI; p=0.00, strong) between the two regulations in limiting sodium levels in instant noodles. This study provides an overview of sodium levels in processed food in Indonesia. The sodium content in most products including instant noodles, as one of the most frequently consumed products, are still above the recommended value. Therefore, it is necessary to develop sodium benchmarkof wider range of food categories in national level that might contribute to sodium intake, as well as for instant noodles. In order to achieve this goal, involvement of multi stakeholder among government, food industry and expert are also needed to deliver effective policies regarding sodium intake concerns.
Co-Authors A.A. Ketut Agung Cahyawan W Abidah, Puri Adzrok Adurrasyid, Zaid Afifah, Diana N. Afrilia Sandra Ramadhani Agata Tantri Atmaja Ahmad Sulaeman Ahmad Syauqy Alamsah Firdaus Alfarisi, Hamzah Ali, Muhammad Saddam Amilia Dayatri Uray Anak Agung Istri Sri Wiadnyani Ananda Putri Cahyani Ananda Putri Cahyani Andi Early Febrinda Ani Karmila Anjani, Gemala Ans Budi Hartanta Ansarullah, Alfia ANZS BUDY HARTANTA Arif - Hartoyo Armando M Saragih Ati Widya Perana Ayu P.G Prayudani Aziz, Sandra Azra, Azka Lathifah Zahratu B.A. Susila Santosa Bambang Purwantara Bella Dinar Fauqii Cahyani Bernadetha Beatrix Sibarani C Hanny Wijaya Cahyani, Ananda Putri Chitisankul, Wanida T. Dadang Supriatna Dadang Supriatna Dadang Supriatna Dadi Hidayat Maskar, Dadi Hidayat Dahrulsyah - - Damayanti, Aprilia F. Deddy - Muchtadi DEDDY MUCHTADI Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Deddy Muchtadi Diana Nur Afifah, Diana Nur Diini Fithriani Dini Wulan Dari Dodik Briawan Dwi Febiyanti - Dwi Utami, Septi Eiichiro Fukusaki Elis Nurhayati Endang Mahati Endang Prangdimurti Evy Damayanthi Fanie Herdiani Fithriani, Diini Fransiska R Zakaria Hadi Riyadi Hadi Susilo Arifin Hadiningtias, Primanisa Hamzah Alfarisi Hardinsyah Hazmi, Khaidar Herpandi . Hidayati, Mustika I Komang Gede Wiryawan Ichsani, Nadya Ikeu Tanziha Inas Suci Rahmawati Indira Saputra Intan Kusumawati Irma Isnafia Arief Isnafia Arief , Irma Istiqomah, Nurani Jefriaman Sirait Karnila, Rahman Karnila Ketut Adnyane Mudite Khaidar Hazmi koekoeh santoso Komang G Wiryawan Komari Komari Komari Komari Lasmiati, Ni Nengah Laut, Bimaris Tranoya Leonita Maulidyanti LUSIA YUNI HASTANTI Made Darawati Manalu, Johanes Marojahan Mardhiyyah, Yunita Siti Maryani Suwarno Maryani Suwarno Maryani Suwarno Maulidyanti, Leonita Muchtadi, Deddy Muhamad Firdaus Muhamad Firdaus Muhamad Syukur Muhammad Agus Muljanto Muhammad Aries Muhammad Ichsan Mursyid . Mursyid Mursyid Mustika Hidayati Nadya Ichsani Nafisah Nancy Dewi Yuliana nFN Akhyar Ni Nengah Lasmiati Novita, Rias R. Nur Wulandari Nurhayati Arifin Nurhayati H.S. Arifin Nurhayati Nurhayati Nurhayati, Elis Nurina Rachma Adiningsih Palupi, Nurheni Sri Perana, Ati Widya Prasetyawati, Renny Candra Prayudani, Ayu P G Prayudani, Ayu Putri Gitanjali Prima Yaumil Fajri Putri, Anisya Saeila Putri, Sastia P. Putri, Sastia Prama Putty Anggi Lestari Rachma Adiningsih, Nurina Rafidha Irdiani Rahmawati, Irma Sarita Rahmawati, Siti Irma Ramadhani, Afrilia Sandra Ramdhani, Rizal Pauzan Ratnaningsih Eko S. Renny Candra Prasetyawati Rimbawan , Rini Kesenja Rita Khairina RR. Ella Evrita Hestiandari Rudy R Nitibaskara Sadiah, Siti Saithong, Pramuan Salsabila Salsabila Sam Herodian Sandra Arifin Aziz Saputra, Indira Saragih, Armando M SARASWATI SARASWATI Sarwono Waspadji Sarwono Waspadji Sarwono Waspadji Sastia Prama Putri Septi Dwi Utami Setyawati S Karyono Setyawati S. K. Setyawati S. Karyono Setyawati, Amalia Rani Sibarani, Bernadetha Beatrix Siti Harnina Bintari Siti Sa'diah Siti Sadiah Slamet Widodo Soewarno S Soekarto Soewarno Soekarto Sri Anna Marliyati Sri Rahmatul Laila Sri Widowati Sri Widowati Sugeng Heri Suseno Sukarno Sukarno Suliantari . Sundari, Fitria Suci Suratno, Yuhlanny Dewi Sussi Astuti Sutrisno Koswara Taopik Ridwan TATI NURHAYATI Tika Pratiwi Khumairoh Tita Aviana Tjahja Muhandri Tryas, Anisha Ayuning Tuti Wresdiyati Tutik Wresdiyati Utami, Sri Inten V Prihananto Vera Di Nurwati Winiati P. Rahayu Winiati Pudji Rahayu Wirawanti, Ika Wirya Wresdiyati, Tuti Yana Nurdiana Yeni Setiorini Yeni Setiorini Yenni MS Nababan Yuhlanny Dewi Suratno Yuspihana Fitrial