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Optimalisasi Deteksi Dini Kanker Serviks Melalui Peningkatan Kapasitas Tenaga Kesehatan dan Edukasi Komunitas di Puskesmas Ngariboyo, Kabupaten Magetan Sari, Aditya Sita; Rahniayu, Alphania; Kurniasari, Nila; Rahaju, Anny Setijo; Mastutik, Gondo; Fauziah, Dyah; Kusumastuti, Etty Hary; Susilo, Imam; Sandhika, Willy; Mustokoweni, Sjahjenny; Ilmiah, Khafidhotul
Jurnal Pengabdian Masyarakat Bhinneka Vol. 4 No. 1 (2025): Bulan September
Publisher : Bhinneka Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58266/jpmb.v4i1.549

Abstract

Latar belakang: Kanker serviks merupakan masalah kesehatan global dengan angka morbiditas dan mortalitas yang tinggi, termasuk di Indonesia. Deteksi dini melalui pemeriksaan Pap smear masih menjadi metode yang sering dipakai, namun akurasi hasilnya sangat bergantung pada teknik pengambilan sampel yang adekuat. Tujuan: Pengabdian masyarakat ini bertujuan untuk Meningkatkan pengetahuan dan keterampilan tenaga kesehatan di Puskesmas Ngariboyo dalam teknik pengambilan sediaan Pap smear yang memadai, dan Meningkatkan kesadaran dan pengetahuan masyarakat tentang deteksi dini kanker serviks. Metode: Metode kegiatan terdiri dari dua bagian utama. Pertama, pelatihan bagi tenaga kesehatan yang meliputi webinar dan praktikum offline untuk mengukur adekuasi sediaan berdasarkan Sistem Bethesda. Kedua, penyuluhan dan skrining offline bagi 95 warga dan 101 peserta pemeriksaan Pap smear. Hasil Hasil pelatihan menunjukkan peningkatan signifikan pengetahuan tenaga kesehatan (nilai 80-100 meningkat dari 58,82% menjadi 82,35%). Penyuluhan juga berhasil meningkatkan pengetahuan masyarakat (nilai 80-100 meningkat dari 80% menjadi 94,74%). Hasil skrining menunjukkan 100% sediaan adekuat dan 100% hasil Negative for Intraepithelial Lesion or Malignancy (NILM). Kesimpulan: Disimpulkan bahwa program ini efektif dalam meningkatkan kapasitas tenaga kesehatan dan kesadaran masyarakat, sekaligus menghasilkan data dasar skrining kanker serviks yang berkualitas bagi wilayah Ngariboyo.
Ekspresi p16 dan CDK4 pada Berbagai Stadium T Karsinoma Laring Diana Purnamasari; Dyah Fauziah
Majalah Patologi Indonesia Vol. 29 No. 1, Januari 2020
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v29i1.404

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BackgroundDefect in cell cycle control affect cell proliferation and play an important role in pathogenesis of cancer. Recently, some proteins areknown to have an influence in cell cycle proliferation. P16 and CDK4 may have influenced in laryngeal carcinoma. CDK4, anoncogene, can form cyclin D1-CDK4 complex that inactivates pRb so that the cell cycle goes from G1 phase to S phase. P16, atumor suppressor protein, can prevent cyclin D1-CDK4 complex so that inhibit cell proliferation.MethodsThis research was an observational analysis study with paraffin block samples, consists of 3 samples of stage T1 and each 10samples of stage T2, T3 and T4 in Laboratory of Anatomical Pathology of RSUD dr. Soetomo that was collected from 2013-2015.Samples were stained with antibody of p16 and CDK4. P16 and CDK4 expression were assessed based on the percentage andintensity of tumor cells that were stained. The differences between variables were analyzed by Kruskal-Wallis. The correlationbetween variables was analyzed by Spearman correlation test.ResultsP16 expression of carcinoma larynx T1 (88.33±7.64), T2 (85.00±5.27), T3 (61.00±21.83), and T4 (62.00±25.30). Besides CDK4expression of carcinoma larynx T1 (43.33±15.28), T2 (47.00±14.18), T3 (75.00±8.50), and T4 (70.00±9.43). Statistic analysisshowed significant differences between p16 and CDK4 expression on each stade of carcinoma larynx (p=0.017 and p=0.000). Andthere were corelation between p16 and CDK4 expression on each stage of carcinoma larynx (p=0.000 and p=0.000).ConclusionThe lower p16 expression and the higher CDK4 expression showed the higher stage of carcinoma larynx.
Analysis of Minichromosome Maintenance-2 (MCM-2) and Cyclin D1 Expression in Meningioma Kurniawati, Yuliana; Fauziah, Dyah
Folia Medica Indonesiana Vol. 54, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

World Health Organization histopathological grading of meningioma is associated with recurrence and clinical outcome. Grade II meningioma can be difficult to distinguish with grade I especially in case in which mitosis is not easily identified. MCM-2 and Cyclin D1 play crucial role in cell cycle and have been reported overexpressed in many malignant tumors. The aim of this observational analytic study was to analyze the differences and correlation between MCM-2 and Cyclin D1 in various meningioma grading. Immunohistochemystry with MCM-2 and Cyclin D1 was performed on 25 paraffin blocks of grade I, II and III meningiomas at the Laboratory of Anatomical Pathology, Dr. Soetomo Hospital. The immunoexpression are evaluated using Labelling Index, then analyzed statistically. The results showed significant difference in expression of MCM-2 within various meningioma grading (p=0.000) and significant difference in expression of Cyclin D1 within grade I and II also within grade I and III (p<0.050), but not in grade II and III (p>0.050). There was also positive correlation between MCM-2 and Cyclin D1 expression in various meningioma grading (rs=0.683, p=0.000). MCM-2 may play role in distinguishing various meningioma grading. Cyclin D1 can distinguish grade I and II also grade I and III, but not grade II and III. Overexpression MCM-2 was along with Cyclin D1 in various meningioma gradings.
Analysis of Maspin Expression in Invasive Ductal Carcinoma of the Breast on Stages IIA and IIIB Hardika, Arif Satria; Fauziah, Dyah
Folia Medica Indonesiana Vol. 54, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

Mammary Serine Protease Inhibitor (maspin) is a tumor suppressor gene, a member of the serine protease inhibitor (serpin) family that works by inhibiting motility of cell movement, invasion and metastasis. Maspin expression is expected to be a prognostic factor as well as a predictive factor in mammary tumors. However, in some recent studies, maspin has a variety of expressions. Although it is known that no maspine appears as an indicator of tumor progression and metastasis, recent study has shown that maspine expression is associated with an aggressive phenotype of breast cancer and with a poor prognosis. Correlations between maspine expression and poor prognosis have also been reported in pancreatic, ovarian, thyroid, bladder and lung cancers. Knowledge of the expression and role of this maspin as well as its relationship with the pathogenesis of breast invasive ductal carcinoma is still small. The aim of this study was to look at differences in maspin expression in breast-invasive ductal carcinoma of stage IIA and stage IIIB groups. This research method used analytic observational research with cross sectional approach. The samples were invasive carcinoma of NST paraffin at the Department of Anatomic Pathology of Dr Soetomo Hospital, Surabaya, from January to December 2015. Thirty samples were divided into two groups, namely stage IIA and stage IIIB groups and immunohistochemical examination with maspin antibody was carried out. The difference of maspin expression in stage IIA and stage IIIB was analyzed using Mann-Whitney statistic test. There were significant differences in maspin expression between stage IIA and stage IIIB groups, where stage IIA has a high maspin expression rather than stage IIIB.
A case of Lhermitte-Duclos disease: the classical “tiger stripes” in magnetic resonance imaging Sensusiati, Anggraini Dwi; Apriawan, Tedy; Fauziah, Dyah
Medical Journal of Indonesia Vol. 35 No. 1 (2026): March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.cr.258118

Abstract

Dysplastic gangliocytoma or Lhermitte-Duclos disease (LDD), is a rare benign cerebellar tumor often presenting with nonspecific symptoms such as intracranial hypertension, cerebellar dysfunction, or cranial nerve deficits, and associated with phacomatosis. The characteristic “tiger stripe” sign on magnetic resonance imaging (MRI) is highly suggestive of LDD, though nodular medulloblastoma remains an important differential diagnosis. We report a 51-year-old woman with arm and leg phacomatosis who presented with a right cerebellar hypodense lesion on computed tomography extending frontally. MRI demonstrated the hallmark “tiger stripe” appearance, and histopathology following surgery confirmed LDD. On follow-up, despite evidence of a recurrent mass on MRI, her cranial nerve paresis improved postoperatively. Imaging revealed hypointense striations on T1 and alternating linear hyperintensities on T2 sequences, obscuring the cerebellar folia, features that distinguish LDD from other cerebellar tumors. This case underscores the diagnostic value of MRI, emphasizing early recognition to prevent misdiagnosis and guide surgical planning.
Profile of Glioma Patients at Dr. Soetomo General Academic Hospital, Surabaya, Indonesia, 2018-2022 Rizki Ahmad Fauzan; Djohan Ardiansyah; Dyah Fauziah; Yudha Haryono
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 17 No. 1 (2026): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V17I12026.74-80

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Highlights: This study provides updated insights into glioma profiles in Indonesia, focusing on a large patient cohort from a major referral hospital. Most gliomas were large tumors located in the cerebrum, with midline shift and heterogeneous contrast enhancement being the primary radiological features observed. Glioblastoma was the predominant type, yet no significant correlation was found between radiological features and histopathological grading, highlighting a potential gap in predictive diagnostics.   Abstract Introduction: Glioma is a common brain tumor that significantly impacts patients’ quality of life by affecting motor, sensory, cognitive, and emotional functions. This study aimed to provide a holistic and comprehensive analysis of glioma characteristics by examining clinical, radiological, and histopathological data from patients treated at Dr. Soetomo General Academic Hospital, Surabaya, Indonesia, over five years from 2018 to 2022. Methods: This retrospective cross-sectional study analyzed records of 204 glioma patients from Dr. Soetomo General Academic Hospital, Surabaya, Indonesia (2018-2022). Data included demographics, clinical symptoms, tumor size and location, radiological features, and histopathological grading. Statistical analysis used Fisher’s exact test (p<0.05), and data were processed using the International Business Machines Corporation (IBM) Statistical Package for the Social Sciences (SPSS) version 23.0. Results: The most affected age group was 30-39 years old (19.1%), and it was predominantly male (59.8%). Headache was the most common presenting symptom (33.8%). Tumors were predominantly located in the cerebrum (75.9%), and most lesions measured >4 cm (76.9%). Midline shift was observed in 69.1% of cases. Contrast enhancement was described as heterogeneous in 35.8% and homogeneous in 1.5%, while 62.7% of cases lacked enhancement data. Histopathologically, the World Health Organization (WHO) grade 4 gliomas (glioblastoma) were the most frequent (39.22%). No significant association was found between radiological features (midline shift or contrast enhancement) and histopathological grading (p>0.05). Conclusion: Gliomas were predominantly found in male patients and were most common among individuals in their younger adult years. The primary clinical symptom was headache, with tumors frequently located in the cerebrum and typically presenting as large masses. Histopathologically, glioblastoma represented the most frequent and aggressive type identified.
Ekspresi Protein HER-2/neu pada Berbagai Tipe Karsinoma Ovarium Barliana Barliana; Faroek Hoesin; Dyah Fauziah
Majalah Patologi Indonesia Vol. 23 No. 1, Januari 2014
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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Background HER-2/neu is second family of epidermal growth factor receptor (EGFR). Amplification of HER-2/neu gene presented in some carcinoma, especially breast carcinoma. Due to increasing incidency of ovarian carcinoma and its poor prognosis because of chemoresistancy, a study on HER-2/neu expression on ovarian carcinoma is conducted. Methods An observasional analytic design with cross sectional approach. Population and samples were paraffin blocks of many type ovarian carcinoma from archives in the Laboratory of Anatomical Pathology of Dr. Soetomo General Hospital, during January-December 2012. Fourty seven samples were obtained and stained with HER-2/neu antibody. HER-2/neu expression assessed by semiquantitative gradation of intensity. Difference of HER-2/neu expression in many type ovarian carcinoma was analyzed with Chi-Square test by probabilitas exact . Results There were 47 cases with mean age 48.51 years (range 19-68 years). Fourteen cases (29.8%) showed HER-2/neu overexpression (score 3+), Statistical analysis showed significant difference of HER-2/neu expression in ovarian carcinoma (p=0.000; coefficient contingency = 0.606). It was showed musinous and clear cell type had more overexpression of HER-2/neu. Conclusion There was HER-2/neu overexpression in ovarian carcinoma especially in mucinous and clear cell type. Key words: clear cell, endometrioid, HER-2/neu, mucinous, ovarian carcinoma, serous.
Ekspresi HER-2/neu dan Ki-67 pada Karsinogenesis Endometrium Febrina Diah Puspitasari; Faroek Hoesin; Dyah Fauziah
Majalah Patologi Indonesia Vol. 23 No. 1, Januari 2014
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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Background Endometrial cancer is the most prevalent gynecological cancer in the Western World. In Dr. Soetomo Hospital, endometrial carcinoma is the second commonest cancer affecting women. HER-2/neu is a receptor on the cell surface which activate cellular proliferation. Ki-67 protein is present in the nucleus of cells that are actively undergoing proliferation. The role of HER-2/neu and Ki-67 overexpression in carcinogenesis of endometrial carcinoma is not clear. This study is to determine the difference of HER-2/neu and Ki-67 expression in normal endometrium, simple hyperplasia without atypia, and endometrial carcinoma endometrioid. Methods This study used cross sectional design. Samples were retrieved from Pathology archives at Dr. Soetomo Hospital during January 2011-December 2011. Normal endometrium in proliferation phase, simple hyperplasia without atypia, and endometrial carcinoma endometrioid were collected and stained with immunohistochemistry using HER-2/neu and Ki-67. Statistical analysis with Kruskal-Wallis and Mann Whitney were performed. Result There were 9 samples with glandular proliferation, 18 samples with simple hyperplasia without atypia, and 18 samples with endometrial carcinoma endometrioid type. Statistical analysis showed no significant difference of HER-2/neu expression in proliferation phase, simple hyperplasia without atypia, and endometrial carcinoma endometrioid (p=0.054) but Ki-67 expression had significant difference in proliferation phase, simple hyperplasia without atypia, and endometrial carcinoma endometrioid (p=0.001). Conclusion There was not significant difference in HER-2/neu expression but significant difference in Ki-67 expression on glandular proliferation, simple hyperplasia without atypia, and endometrial carcinoma endometrioid. Key words : endometrioid, endometrium, HER-2/neu, Ki-67, simple hyperplasia without atypia.
Pola Ekspresi Vascular Endothelial Growth Factor (VEGF) Dikaitkan dengan Pertumbuhan Tumor dan Edema Peritumoral pada Astrositoma Diah Prabawati Retnani; Dyah Fauziah
Majalah Patologi Indonesia Vol. 22 No. 3, September 2013
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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ABSTRAK Latar belakang Astrositoma adalah tumor dari sel astrosit dengan variasi derajat histopatologik, disertai ukuran edema peritumoral. Peningkatan derajat keganasan, ukuran tumor dan pembentukan edema peritumoral diperlukan proses angiogenesis. Vascular Endothelial Growth Factor (VEGF) adalah salah satu faktor penting dalam proses angiogenesis. VEGF diperlukan dalam proliferasi, survival dan migrasi sel endotel dan permiabilitas vaskuler. Peranan VEGF dalam pembentukan edema peritumoral masih dalam perdebatan. Penelitian ini bertujuan untuk menganalisis hubungan antara pola ekspresi VEGF dan peningkatan derajat histopatologik, ukuran tumor dan insiden edema peritumoral pada astrositoma. Metode Arsip histopatologik astrositoma derajat II, III, IV (Glioblastoma) dari RSUD Dr. Soetomo Surabaya selama kurun waktu Januari 2009 hingga Januari 2012 dikumpulkan. Pada tiga puluh kasus astrositoma yang memenuhi kriteria inklusi dilakukan pemeriksaan imunohistokimia dengan VEGF Rabbit. Hubungan pola ekspresi VEGF dan derajat histopatologik, ukuran tumor dan kejadian edema peritumoral dianalisis menggunakan uji Spearman. Hasil Sebanyak 29 dari 30 kasus menunjukkan ekspresi VEGF positif. Dari 29 kasus dengan ekspresi VEGF positif tersebut, 13 kasus adalah astrositoma derajat IV, 22 kasus dengan ukuran tumor > 2cm, dan 17 kasus disertai edema peritumoral. Analisis statistik ekspresi VEGF memiliki pengaruh terhadap peningkatan derajat histopatologik (p=0,023), rs=0,413) dan ukuran tumor (p=0,005, rs= 0,499) namun tidak berpengaruh terhadap kejadian edema peritumoral (p=0,273). Kesimpulan Penelitian ini mengungkapkan bahwa ekspresi VEGF dapat meningkatkan derajat histopatologik dan besar tumor, namun tidak berpengaruh terhadap pembentukan edema peritumoral pada astrositoma. Kata kunci: Astrositoma, VEGF, derajat histopatologik, edema peritumoral. ABSTRACT Background Astrocytoma is a glial tumor that derived from astrocyte cells with various tumor size, histopathologic grading and peritumoral edema. Angiogenesis plays role in tumor grading, tumor size and formation of peritumoral edema. VEGF is needed for proliferation, survival, migration of endothel cell and vascular permiability. The role of VEGF to regulate peritumoral edema in brain tumor is still debated. The purpose of this study was to analyze the relationship between VEGF expression and tumor size, histopathologic grading and peritumoral edema of astrocytoma. Methods Pathology archives of grade II, III and IV astrocytomas were retrieved at Dr Soetomo Hospital Surabaya between Januari 2009-Januari 2012. Thirty cases of astrocytomas that fulfilled the conclusion criteria were stained with VEGF rabbit monoclonal antibody (clone EP1176Y). Relationship between VEGF expression and histopathologic grading, tumor size and peritumoral edema were analyzed using Spearman correlation test. Results There were 29 of 30 cases of astrocytom showing positif VEGF expression, 13 cases of grade IV astrocytoma, 22 cases had tumor size more than 2 cm, and 17 cases showing peritumoral edema. Statistical analyzis revealed that VEGF expression had a significant association with histopathologic grading ( p=0.023; rs=0.413) and tumor size (p=0.005; rs=0.499). In contrast, VEGF expression had no significant assoiation with peritumoral edema (p=0.273). Conclusion This study revealed that VEGF plays role in increasing histopathologic grading and tumor size in astrocytoma but its role in peritumoral edema is not significant. Key words : astrocytoma, VEGF, histopathologic grading, peritumoral edema.
Ekspresi Epidermal Growth Factor Receptor (EGFR) dan Ki-67 pada Astrositoma Fitriana .; Dyah Fauziah
Majalah Patologi Indonesia Vol. 24 No. 3, September 2015
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

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ABSTRAK Latar belakang Insiden astrositoma di RSUD Dr. Soetomo Surabaya meningkat dalam tiga tahun terakhir. Keterlibatan berbagai growth factor dan reseptornya serta penanda proliferasi dalam patogenesis molekuler neoplasma astrocytic banyak diteliti secara ekstensif. Epidermal growth factor receptor (EGFR) dan Ki-67 terlibat dalam transduksi sinyal mitogenik dan jalur proliferasi sel. Tujuan penelitian ini menganalisis ekspresi EGFR dan Ki-67 pada astrositoma derajat II,III, dan IV, serta menganalisis korelasi antara ekspresi EGFR dan Ki-67 pada derajat histopatologi astrositoma. Metode Rancangan penelitian yang digunakan adalah cross sectional. Sampel dibagi dalam; 6 astrositoma derajat II, 7 astrositoma derajat III, and 13 astrositoma derajat IV yang telah didiagnosis selama periode 2009-2013. Sampel dilakukan pulasan immunohistokimia dengan antibodi monoklonal EGFR dan Ki-67. Perbedaan ekspresi EGFR dan Ki-67 pada berbagai derajat astrositoma dianalisa menggunakan uji Kruskal-Wallis. Hubungan antara ekspresi EGFR dan Ki-67 pada astrositoma dianalisa menggunakan uji Spearman. Hasil Hasil analisa statistik ekspresi EGFR pada astrositoma derajat II, III, dan IV menunjukkan perbedaan bermakna (nilai p=
Co-Authors Agung Dwi Suprayitno Agustin, Leonita Alpha Fardah Athiyyah Alphania Rahniayu Anak Agung Gede Sugianthara Anang Endaryanto Andi Syarti Anggoro, Adhitri Anny Setijo Rahaju, Anny Setijo Ardiansyah, Djohan Ariani, Grace Arif Satria Hardika Askandar Tjokroprawiro Barliana Barliana Budi Utomo Cindy Angelina Limantara Cita Rosita Sigit Prakoeswa Deasy Fetarayani Dewi Astuti Kurniawati Dewi Sartika A. W. Dian Yuliartha Lestari Djuanda, Stephanie N. Dwirani Rosmala Pratiwi Dyah Erawati, Dyah Edi Suyanto Elvin Nuzulistina Emillia Wijayanti Esti Hindariati Faradila Budi Saputri Faroek Hoesin Febria Rizky Patikawa Febrina Diah Puspitasari Ferdiansyah Mahyudin Fira Soraya Fitriana . Gatot Soegiarto Gilang Adi Firmansyah Gondo Mastutik Gunawan, Vania A. Hardika, Arif Satria Heriyawati, Heriyawati Hermawan Udiyanto Ilmiah, Khafidhotul Imam Susilo INDRA YULIATI, INDRA Ingrid Suryanti Surono Khafidhotul Ilmiah Kurniawati, Yuliana Kusumastuti, Etty H. Kusumastuti, Etty Hari KUSUMASTUTI, ETTY HARY Lefi, Achmad Lies Mardiyana, Lies Lilihata, Jilientasia G. Maharani, Andi RK. Mamiek Dwi Putro MANUEL HUTAPEA MANUEL HUTAPEA, MANUEL Marhana, Isnin A. Mayasari, Diana Mega Kahdina Meyta Riniastuti MUHAMMAD RIZKI YAZNIL Nabilah Puspa Utami Nasrun Bakri Nila Kurniasari Nila Kurniasari Nugraha, Ricardo A. Nugroho, Gilang MS. Nunuk Mardiana, Nunuk Prastiya Indra Gunawan Purnamasari, Diana Qonitatillah, Ana Rahaju, Anny S. Rahmadewi Rahmadewi Retnani, Diah Prabawati Ridholia Ridholia Ridholia Ridholia, Ridholia Rinjani, Lalu GP. Rizki Ahmad Fauzan Rizkie T Trisnawaty Rosyid, Alfian N. ROY YUSTIN SIMANJUNTAK ROY YUSTIN SIMANJUNTAK, ROY YUSTIN S.Pd. M Kes I Ketut Sudiana . Sarah Fauzia Sari, Aditya Sita Semedi, Bambang P. Sensusiati, Anggraini Dwi Sjahjenny Mustokoweni, Sjahjenny SUHATNO - SUHATNO -, SUHATNO Supriadi Supriadi Sutrisno Sutrisno Tedy Apriawan Teguh Prakosa, Teguh Tri Wulanhandarini Ulinta Pasaribu Ummi Maimunah wahjoe djatisoesanto Wahyu, Dwi wihasto suryaningtyas Willy Sandhika Wiratama, Priangga A. Wiratama, Priangga Adi Wirawan, Putu Tanisya Putri Yandi, I KR. Yaznil, Muhammad Rizki Yetti Hernaningsih Yudha Haryono Yuliana Kurniawati Yusuf, Muhtarum