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Chemical Composition and Anticancer Activity Of the Essential Oil Of Surian Heartwood Rita K Sari; Wasrin Syafii; Suminar S Achmadi; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 9, No 2 (2011): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.853 KB) | DOI: 10.51850/jitkt.v9i2.140

Abstract

Chemical constituents of the essential oil of water and steam distillates of surian heartwood (Toona sinensis) were analyzed by a gas chromatograph-mass spectrometer. Then, the oil was investigated in vitro anticancer bioassay for its possible antioxidant activity by DPPH free radical scavenging and antiproliferative effects by MTT method using Raji lymphoma cancer cell lines (ATCC CCL 86), HeLa cervical cancer cell lines (ATCC CCL2), and Vero normal cell lines (ATCC CCL 81). The essential oil yield from water and steam distillates of surian heartwood was 0.4% (w/w). Thirty-two compounds were identified, that consisted mainly of oxygenated sesquiterpenes (77%). The most representative compounds were spathulenol (21%), isospathulenol (7%), α-cadinol (7%), β-cedrenoxide (6%), and torreyol (5%). The oil showed less effective antioxidant, but the oil had high antiproliferative effects on Raji and HeLa cancer cells with IC50 28 and 134 μg ml-1, respectively when compared to Vero normal cells (IC50 1412 μg ml-1). Our findings suggest that the essential oil of surian heartwood might be considered as a potentially anticancer agent on human cancer cells, especially lymphoma cancer cells.Key words: antioxidant activity, antiproliferative effects, cancer cell lines, essential oil, Toona sinensis
Lime Pretreatment on Jabon Wood to Improve Its Reducing Sugar Yield Yusup Amin; Wasrin Syafii; Nyoman J Wistara; Bambang Prasetya
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 2 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.519 KB) | DOI: 10.51850/jitkt.v12i2.75

Abstract

The purpose of this study was to determine the performance of enzymatic hydrolysis on lime pretreated jabon wood (Anthocephalus cadamba Miq). Proceeding hydrolysis process, the morphology and crystallinity changes of pretreated jabon were also examined. In the present study, jabon wood meal was put into digester and then lime was loaded at varied lime loading, i.e. at 0.1, 0.3, and 0.5 g Ca(OH)2 g-1 of dry biomass in 90 ml of water. The slurry was then homogenized and heated in the oil bath at 100, 125 and 150 C for 2, 4 and 6 hours. Enzymatic hydrolysis was performed in accordance to the NREL procedures using a cellulase enzyme. The enzyme loading variation was 10, 20 and 40 FPU g-1 of the substrate. the RSY of pretreated jabon increased up to 9.7 folds from that of its control. The highest RSY was obtained for pretreatment with 0.5 g Ca(OH)2 g-1 dried biomass at 150 °C for 4 hours and hydrolyzed with 40 FPU of cellulase (118 mg equivalent glucose g-1 dry biomass). This yield was comparable with 14.4% More than a third of the lignin and hemicellulose content (45.83 and 65.52%) were degraded by lime pretreatment.Keywords: enzymatic hydrolysis, jabon wood, lime pretreatment, reducing sugar yield
Sifat Kimia dan Dimensi Serat Kayu Mangium (Acacia mangium Willd.) dari Tiga Provenans. Chemical Properties and Fiber Dimension of Acacia mangium Willd. from Three Provenances Wasrin Syafii; Iskandar Z Siregar
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 4, No 1 (2006): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.477 KB) | DOI: 10.51850/jitkt.v4i1.286

Abstract

The objectives of this research are to evaluate the chemical components and the fiber dimension of Acacia mangium Willd from three provenances.  The wood samples used in this study are grown in Parungpanjang, Bogor originated from three provenances namely Queensland (QLD), Papua New Guinea (PNG), and East Indonesia (EI). The chemical components measured in this study are cellulose, hemicelluloses, lignin, and extractives, while fiber dimensions and its derivatives determined are Runkle Ratio, Felting Power, Muhlsteph Ratio, Flexibility Ratio, and Coefficient of Rigidity. This research indicated that among three provenances investigated, the Queensland provenance is better than two others as sources of raw material for pulp and paper due to the highest cellulose content and lowest lignin and extractives contents. Likewise, from fiber dimension aspect, Queensland provenance also showed better properties than that of PNG and EI
Sifat Anti Jamur Kayu Kupa (Syzygium polycephalum (Mig)) (Antifungal Properties of Kupa Wood (Syzygium polycephalum Mig.)) Renhart Jemi; Wasrin Syafii; Fauzi Febrianto; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 8, No 2 (2010): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.91 KB) | DOI: 10.51850/jitkt.v8i2.214

Abstract

The objectives of this research were to evaluate the extractive substances of heartwood of Kupa wood (Syzygium polycephalum (Mig)) and its potention as bio-active substance to wood destroying fungi i.e., Schizophyllum commune Fr and Pleurotus sp. Successive extraction method was used in this experiment. The heartwood part was then converted into 40 mesh wood flour and followed by extraction using methanol, chloroform, ethyl acetate, n-hexane and buthanol solvents. The extractives obtained were subjected to wood destroying fungi S. commune and Pleurotus sp. The results indicated that the extractive content of S. polychephalum mostly dominated by substance diluted in chloroform (2.87%), followed by ethyl acetate (0.38%), n-hexane (0.33%) and buthanol (0.05%). All the wood extracts of S. polychephalum potentially contain anti-fungal compound to inhibit the growth of S. commune Fr and Pleurotus sp fungi. N-hexane and ethyl acetate extracts of S. polychephalum are the most active extracts. Isolation of ethyl acetate fraction resulted in nine (9) active compounds (G.1-G.9) that could inhibit the growth both S. commune and Pleurotus sp with IC(50) values 49.33-61.71 ppm and 48.84-64.61 ppm, respectively. It was found that G.2 compound of ethyl acetate has anti fungal substance namely 3-O-glucosyl-3’,4’ 5-trihydroxyflavonol.
Aktivitas Antidiabetes Ekstrak Etanol Jabon (Anthocephalus cadamba) (Antidiabetic Activity of Jabon (Anthocephalus cadamba) Ethanol Extracts) Laela N. Anisah; Wasrin Syafii; Rita K. Sari; Gustan Pari
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 13, No 2 (2015): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.939 KB) | DOI: 10.51850/jitkt.v13i2.28

Abstract

Diabetes mellitus (DM) merupakan penyakit gangguan metabolik yang menjadi masalah utama kesehatan di dunia termasuk Indonesia. Penelitian ini bertujuan untuk menetapkan rendemen zat ekstraktif tanaman jabon,  aktivitas antidiabetesnya secara in vitro  terhadap enzim -glukosidase serta menganalisis kandungan kimia ekstrak teraktifnya   Ekstrak jabon dihasilkan dari proses maserasi dengan etanol 95% pada berbagai bagian pohon (daun, kulit, kayu). Hasil penelitian menunjukkan bahwa kadar ekstrak tertinggi terdapat pada bagian daun (16,5%), diikuti bagian kulit (4,62%) dan kayu (2,04 %). Berdasarkan uji aktivitas penghambatan enzim   -glukosidase, ekstrak etanol daun jabon merupakan ekstrak teraktif dengan nilai IC50 7,24 µg ml-1 (sangat aktif), sedangkan ekstrak etanol bagian kulit dan kayu tergolong  tidak aktif (IC50 > 100  µg ml-1). Hasil uji fitokimia secara kualitatif menunjukkan kelompok senyawa yang terkandung di dalam ekstrak etanol daun jabon adalah flavonoid, hidroquinon, saponin, tannin, alkaloid, terpenoid dan steroid yang  diduga  berperan  dalam  menghambat  aktivitas  enzim    -glukosidase.  Analisis  GCMS mendeteksi adanya senyawa fenolik asam quinat dan katekol serta turunan asam lemak (asam heksadekanoat metil ester) yang diduga memiliki aktivitas antidiabetes. Berdasarkan hasil tersebut, ekstrak etanol daun jabon sangat berpotensi sebagai sumber obat andiabetes.
Sifat Anti Jamur Kayu Kupa (Syzygium polycephalum (Mig)) (Antifungal Properties of Kupa Wood (Syzygium polycephalum Mig.)) Renhart Jemi; Wasrin Syafii; Fauzi Febrianto; Muhammad Hanafi
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 8, No 2 (2010): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.91 KB) | DOI: 10.51850/jitkt.v8i2.224

Abstract

The objectives of this research were to evaluate the extractive substances of heartwood of Kupa wood (Syzygium polycephalum (Mig)) and its potention as bio-active substance to wood destroying fungi i.e., Schizophyllum commune Fr and Pleurotus sp. Successive extraction method was used in this experiment. The heartwood part was then converted into 40 mesh wood flour and followed by extraction using methanol, chloroform, ethyl acetate, n-hexane and buthanol solvents. The extractives obtained were subjected to wood destroying fungi S. commune and Pleurotus sp. The results indicated that the extractive content of S. polychephalum mostly dominated by substance diluted in chloroform (2.87%), followed by ethyl acetate (0.38%), n-hexane (0.33%) and buthanol (0.05%). All the wood extracts of S. polychephalum potentially contain anti-fungal compound to inhibit the growth of S. commune Fr and Pleurotus sp fungi. N-hexane and ethyl acetate extracts of S. polychephalum are the most active extracts. Isolation of ethyl acetate fraction resulted in nine (9) active compounds (G.1-G.9) that could inhibit the growth both S. commune and Pleurotus sp with IC(50) values 49.33-61.71 ppm and 48.84-64.61 ppm, respectively. It was found that G.2 compound of ethyl acetate has anti fungal substance namely 3-O-glucosyl-3’,4’ 5-trihydroxyflavonol.
Bioactivity Test of Mindi Extractives (Melia azedarach Linn) Using Brine Shrimp Lethality Test Wasrin Syafii; Rita K Sari; Siti Maemunah
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 1 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.924 KB) | DOI: 10.51850/jitkt.v12i1.82

Abstract

The aims of this research were to determine the yield of extracts from continuous extraction of heartwood, sapwood, inner bark, branch, and leave of Toona sinensis in n-hexane, ethyl acetate, and methanol solvents and their bioactivity using brine shrimp lethality test (BSLT) was evaluated. The results indicated that the yield of leave, inner bark, branch, heartwood, and sapwood extracts were 11.0, 6.7, 1.9, 1.6, and 1.6%, respectively. The highest yield of extracts was obtained from extraction in ethyl acetate (16.1%), followed by methanol (3.5%), and n-hexane (3.1%). The BSLT tests showed that the ethyl acetate extracts of all tree parts have higher bioactivities (LC50 1-52 μg ml-1) than the n-hexane extracts (LC50 40-181 μg ml-1), and the methanolic extracts (LC50 49-1375 μg ml-1). The ethyl acetate extract from inner bark has the highest bioactivity (LC50 μg ml-1). The qualitative analysis detect the ethyl acetate extract from inner bark containing saponins, alkaloids, phenolics, flavonoids, triterpenoids, steroids, and glycosides.Key words: Artemia salina, bioactivity, brine shrimp lethality test, Toona sinensis
Pengaruh Durasi Steam terhadap Kualitas Arang Aktif Limbah Sagu (The Effect of Steam Duration on Quality and Characteristics of Activated Charcoal of Sago Waste) Herman Siruru; Wasrin Syafii; Nyoman J Wistara; Gustan Pari
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 16, No 2 (2018): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3037.633 KB) | DOI: 10.51850/jitkt.v16i2.447

Abstract

Sago waste is a potential biomass that can be used as active charcoal raw material. The objectives of this research were to know the effect of steam duration on quality and characteristics of activated charcoal of sago waste. Carbonization process was carried out at 400 ⁰C for four hours, then activation was carried out at 100 ml bar steam pressure, at a temperature of 800 ⁰C with variations of steam duration 60, 80 and 100 minutes and activation without steam, only heating for 60 minutes. The proximate test used the SNI standard, observed the functional group using Fourier Transform Infrared Spectrophotometer (FTIR) and observed the active charcoal structure using X-ray diffraction (XRD). The results showed that the yield, moisture content, volatile, ash content, fixed carbon, iodine absorption of activated charcoal of sago bark and sago soft core were, respectively, 39.22–84.10%, 4.63–6.39%, 3.03–5.06%, 7.74–33.43%, 61.54–88.63%, 158.60–972.60 mg g-1. XRD analyzes showed that the degree of crystalline activated charcoal of sago bark waste with the highest steam time of 100 minutes (35.98%) compared with the degree of crystalline other activated charcoal is only about 20.57-7.30%. FTIR spectroscopy results from activated charcoal of sago waste also identified nitrogen compounds such as NO­2 at wave numbers 1370-1390 cm-1; 1530-1560 cm-1; 1620-1660 cm-1 and phosphorus compounds such as P-S at 200-500 cm-1 wave number; P=S at the wave number 500-850 cm-1.
Aktivitas Antioksidan dan Toksisitas Akut Zat Ekstraktif dari Residu Penyulingan Surian (Toona sinensis Roemor) Rita K Sari; Desi Melianti; Wasrin Syafii; Dewi R Agungpriyono
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 11, No 2 (2013): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.786 KB) | DOI: 10.51850/jitkt.v11i2.194

Abstract

The objective of this research was to determine the yield, antioxidant activity, acute toxicity, and phytochemical compound of extractives from left over distilling liquid and distillation residues of various part of surian (Toona sinensis). The extracts from the left over distilling liquid of leaves, heartwood, and sapwood were assigned as LL, HL, and SL, respectively, whereas LB, HB, and SB were respectively the extracts from leaves, heartwood, and sapwood of boiled solid residue. The yield of LL, LB, SL, HL, SB, and HB were 28.78, 10.18, 2.11, 1.74, 0.92, and 0.81% respectively. Based on antioxidant activity test, the LB, LL, and HL extracts were classified as very active with EC50 of 5.45, 5.70, and 5.91 mg ml-1 respectively. The SL was classified as active (EC50 32.36 mg ml-1), the SB and HB extracts were classified as inactive (EC50 > 100 mg ml-1). Based on acute toxicity test, LB is the best natural antioxidant because it was non toxic material (LD50 47752 mg kg-1 BW), while the LL and HL extracts were clasified as low toxicity with LD50 4518.56 and 1999.86 mg kg-1 BW (body weight). The LB extract contained phenol hydroquinone, flavonoids, and tannins (total phenolic content: 94 mg g-1 GAE).
The Color Change of Jabon Wood Stained by Bark Extract of Samak Wood (Syzygium inophyllum) Muflihati Muflihati; Deded S Nawawi; Istie S Rahayu; Wasrin Syafii
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 1 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.699 KB) | DOI: 10.51850/jitkt.v12i1.78

Abstract

The water soluble extract of samak (Syzygium inophyllum) bark was applied as natural dye for jabon wood (Anthocephalus cadamba). Ten percent of the extract concentration was used for staining of jabon wood by soaking methods for 74 h at room temperature, and for 3 h at 70 oC, respectively. The stained jabon wood performances were evaluated as the color change and stability by hot water leaching test. The stained and leached woods were assessed by determination of color changes using CIELab method and FTIR analysis. Based on the CIELab analysis, the brightness (L* value) of stained jabon wood decreased, and tend to more reddish (a* value increased) and yellowish (b* value slightly increased) compared to these of unstained wood, as the results, the color of jabon wood changed from light grey to reddish orange after staining with samak bark extract. The leaching test of stained wood to hot water changed the color from reddish orange to dull orange. FTIR analysis indicated that part of the extract stained jabon wood was quite stable to hot water treatment.Key words : bark extract, jabon wood, natural dye, samak wood, Syzygium inophyllum
Co-Authors Abdul Aziz Darwis Achmad Achmad Achmad Achmad Adesna Fatrawana Adesna Fatrawana Akhiruddin Maddu Akhirudin Maddu Akhirudin Maddu Amril Aman Anak Agung Istri Sri Wiadnyani Ani Suryani Anne Carolina Bambang Prasetya Bambang Prasetya Bambang Prasetya Bintang C. H. Simangunsong Buchari Buchari Buchari Buchari Deded Sarip Nawawi Denny Irawati Desi Melianti Dewi R Agungpriyono E Gumbira Sa, E Gumbira Eka Novriyanti Eka Novriyanti Eko Budi Santoso Eko S Pribadi Elis Nina Herliyana ENDANG SUHENDANG Erdy Santosa Erdy Santosa Evie Nihayah Fabianus Ranta Fahriya Puspita Sari Faizatul FALAH Fathul Yusro Fauzi Febrianto Gunawan Pasaribu Gunawan Pasaribu Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustan Pari Gustini Syahbirin Herman Siruru Herman Siruru Hikma Yanti Hiroyuki Yamamoto Husni Y Rosadi, Husni Y I MADE ARTIKA I Nyoman Jaya Wistara IGK Tapa Darma Illah Sailah Imam Wahyudi Indrawan, Imam Wahyudi Irmanida Batubara Irnayuli R. Sitepu Irnayuli R. Sitepu Iskandar Z Siregar Istie Rahayu Izza Firdausi Hadiyanto Jemi, Renhart Juliasman Juliasman Karliati, Tati Khaswar Syamsu Kosei Yamauchi Kurnia Sofyan Kurnia Sofyan Kurnia Sofyan Laela N. Anisah Laela Nur Anisah Latifah K. Darusman Latifah K. Darusman Lucky Risanto M. Daud Maggy T Suhartono Maggy T Suhartono Maman Turjaman Maman Turjaman Maya Ismayati Mody Lempang Mody Lempang Mody Lempang Muflihati Muflihati Muhammad Adly Rahandi Lubis Muhammad Fadli Muhammad Hanafi Nissa Nurfajrin Solihat Norman Razief Azwar Nugraha, Arifin Budiman Nur Adi Saputra Nur Azizah Pudja Mardi utomo Pudja Mardi utomo Purwoko, Agus Rahmi Mauladdini Renhart Jemi Rita K Sari Rita Kartika Sari Romi Irka Putra S Heris Anita Saat Egra Santiyo Wibowo Santiyo Wibowo Santiyo Wibowo Saptadi Darmawan Saptadi DARMAWAN Simon Taka Nuhamara Simon Taka Nuhamara Siruru, Herman Siti Maemunah Siti Sa'diah Siti Sa'diah Soetrisno Hadi Soetrisno Hadi Sri Familasari Suminar A Achmadi Suminar S Achmadi Syamsul Arifin Tohru Mitsunaga Umi Cahyaningsih Widya FATRIASARI Widya Fatriasari Widya Fatriasari Widya FATRIASARI Yanico Hadi Prayogo Yanotama T Laksana Yanti Hikma Yusup AMIN