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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Bios Logos BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Wacana, Jurnal Sosial dan Humaniora Proceeding International Conference on Global Resource Conservation Journal of Mathematical and Fundamental Sciences Jurnal Sumberdaya Hayati Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology REINWARDTIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Forest and Society Floribunda Proceeding Biology Education Conference Makara Journal of Science Reinwardtia Jurnal Biosilampari: Jurnal Biologi

TATIK CHIKMAWATI

Bogor Agricultural University

Author-ID : 247116

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Characterization of Rambutan Cultivars (Nephelium lappaceum) Based on Leaf Morphological and Genetic Markers Manggabarani, Andi Madihah; Chikmawati, Tatik; Hartana, Alex
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12221

Rambutan (Nephelium lappaceum) is an economically important plant which is native to Indonesia and Malaysia. The diversity of rambutan in Indonesia is abundance especially in Kalimantan where the wild relatives still grow naturally. Rambutan cultivars are usually differed from each other based on fruit morphological characters. However, rambutan tree begins to fruiting for the first time in 3-4 years. Therefore, another character is needed to characterize each cultivar in a short period. The objectives of this study were to distinguish rambutan cultivars using leaf morphological and Inter-Simple Sequence Repeat (ISSR). As many as 30 rambutan cultivars collected from Cipaku Orchard and Mekar Sari Park were observed for their morphological and ISSR characters. Six characters of were surveyed for leaf morphological character. For the genetic character, 6 out of 31 ISSR primers were assessed which resulted in 58 polymorphic bands (87%). As a result, leaf morphological characters overlapped among cultivars causing difficulties distinguishing each cultivar. ISSR marker, three major clusters have been identified according to UPGMA method. Index similarity among rambutan accessions from ISSR data ranged from 48-93%. As a conclusion, ISSR marker could be potentially applied rambutan cultivars characterization.

PEMANFAATAN TUMBUHAN OBAT SECARA EMPIRIS PADA SUKU MANDAILING DI TAMAN NASIONAL BATANG GADIS SUMATERA UTARA Nasution, Aswarina; Chikmawati, Tatik; Walujo, Eko Baroto; Zuhud, Ervizal A.M.
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Empirical Utilization of Medicinal Plant on Mandailing Tribe inÂ Batang Gadis National Park North SumatraABSTRACTMandailing tribe is an indigenous tribe that inhabits the area around Batang Gadis National Park (BGNP), North Sumatra. They have knowledge related to the use of plants for traditional medicine. Nevertheless, the information about this local knowledge is not uncover yet. This study aims to reveal the knowledge of the Mandailing tribe in utilizing plants as a traditional medicine. The research location was in 4 villages around BGNP. Data were collected through interviews with respondents and direct survey in the field. Data were analyzed descriptively qualitative. The results showed that there were about 81 plant species used for treatment covered in 38 families to treat 41 types of diseases. The most widely used medicinal plant species are from the Compositae family. Herbs dominant used by the community as a medicinal plant comprised 50 species of plants. The high diversity of medicinal plants indicated that utilization of plants for health is the main priorities of a Mandailing tribe.Keywords: Biodiversity, disease, local knowledge, Mandailing tribe, traditional medicine Â ABSTRAKSuku Mandailing merupakan suku asli yang mendiami kawasan di sekitar Taman Nasional Batang Gadis (TNBG), Sumatra Utara. Mereka memiliki pengetahuan terkait pemanfaatan tumbuhan untuk obat tradisional. Namun informasi terkait pengetahuan lokal tersebut belum diungkapkan. Penelitian ini bertujuan untuk mengungkap pengetahuan Suku Mandailing dalam memanfaatkan tumbuhan sebagai obat tradisional. Lokasi penelitian berada di 4 desa di sekitar TNBG. Pengumpulan data melalui wawancara dengan respoden serta survey langsung di lapangan. Data dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan ada sekitar 81 spesies tumbuhan yang digunakan untuk pengobatan yang tercakup dalam 38 famili untuk mengobati 41 jenis penyakit. Spesies tumbuhan obat yang paling banyak digunakan berasal dari Famili Compositae. Habitus herba dominan digunakan masyarakat sebagai tumbuhan obat yang meliputi 50 spesies tumbuhan. Tingginya keanekaragaman tumbuhan obat menunjukkan bahwa pemanfaatan tumbuhan untuk kesehatan adalah prioritas utama Suku Mandailing.Â Kata Kunci: Biodiversitas, suku Mandailing, obat tradisional, pengetahuan lokal, penyakit

Morphological Diversity of Local Sorghum Cultivar (Sorghum bicolor) of East Nusa Tenggara, Indonesia Tnunay, Ite Morina Yostianti; Chikmawati, Tatik; Miftahudin, Miftahudin
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Sorghum has great potential to be developed in marginal lands of Indonesia including East Nusa Tenggara. However, the information about sorghum diversity in this area was very limited. This research aimed to describe morphological variation, and assessing the value of genetic diversity based on morphological characters of local sorghum cultivar of East Nusa Tenggara. The exploration and sample collection were conducted in 3 islands, i.e.: Timor, Sumba, and Flores. The observation of morphological characters was based on sorghum descriptor. Morphological character similarities were analyzed using Simple Matching (SM) coefficient, and a dendrogram was constructed using Unweighted Pair Group Method with Arithmetic Average (UPGMA) method. The results showed that 36 accessions of sorghum in East Nusa Tenggara varied in 17 morphological characters. At the similarity coefficients of 48%, all sorghum accessions were separated into 2 groups based on the presence or absence of aleurone layer. Group I consisted of 12 accessions with no aleurone layer, while group II consisted of 24 accessions has aleurone layer. The genetic diversity of sorghum of East Nusa Tenggara is low (I=0.62–0.71 and h=0.37-0.42). The genetic diversity between populations (HT=0.4203) is higher than within populations (HS=0.3961). This study provides the first complete information about sorghum diversity in East Nusa Tenggara that will be useful as basic information for sorghum development in this area in the future.

MORFOLOGI SPORA DAN PERKEMBANGAN GAMETOFIT Davallia denticulata dan Davallia trichomanoides Meliza, Rezika; Chikmawati, Tatik; Sulistijorini, Sulistijorini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 6, No 1 (2019): June 2019
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Spore Morphology and Gametophyte Development of Davallia denticulata and Davallia trichomanoidesABSTRACTDavallia denticulata and D. trichomanoides are two attractive and decorative fern species for ornamental. Spore morphology has an important role in fern taxonomy, while media composition has important role in the growth and development of their gametophytes. Such information on the two fern species was lacking. Therefore, this study aimed to reveal the information of the spore morphology and gametophyte developmental stages of D. denticulata and D. trichomanoides on three different media. The spores were collected from Bogor, West Java. The spores were sown in three sterile media. Spore morphology and gametophyte development were observed under a stereoscopic microscope. Both gametophyte species reached their mature stage at 25 weeks after planting on the different media compositions. D. denticulata showed the best gametophyte development, and formed mature gametophytes on the media of vermiculite, sphagnum moss, and perlite, while D. trichomanoides grew best into maturity stage on the media containing vermiculite, and sphagnum moss. Thus, the presence of sphagnum moss in the media is an important material for the growth and development of Davallia gametophyte.Keywords: Davallia, development, gametophyte, growth, media ABSTRAKDavallia denticulata dan D. trichomanoides merupakan dua spesies tumbuhan paku yang menarik dan indah untuk tanaman hias. Morfologi spora memiliki arti penting dalam taksonomi tumbuhan paku, sedangkan komposisi media berperan penting untuk pertumbuhan dan perkembangan gametofitnya. Informasi seputar hal ini terkait dua spesies tumbuhan paku tersebut belumlah ada. Penelitian ini bertujuan untuk mengungkap informasi mengenai ciri morfologi spora dan tahapan perkembangan gametofit D. denticulata dan D. trichomanoides pada tiga komposisi media berbeda. Pengambilan spora dilakukan di Bogor, Jawa Barat. Spora ditumbuhkan pada tiga media steril. Morfologi spora dan perkembangan gametofit diamati menggunakan mikroskop stereo. Kedua spesies memiliki waktu perkembangan terbaik untuk mencapai tahap gametofit dewasa yaitu 25 minggu pada komposisi media yang berbeda. D. denticulata berkembang dengan baik, dan membentuk gametofit dewasa pada media vermiculite, lumut sphagnum, dan perlite. D. trichomanoides berkembang hingga tahap gametofit dewasa dengan baik pada media vermiculite, dan lumut sphagnum. Dengan demikian keberadaan lumut sphagnum pada media sangat penting untuk pertumbuhan dan perkembangan gametofit Davallia.Kata Kunci: Davallia, gametofit, media, perkembangan, pertumbuhan

Pengetahuan Fauna (Etnozoologi) Masyarakat Tengger di Bromo Tengger Semeru Jawa Timur Batoro, Jati; Setiadi, Dede; Chikmawati, Tatik; Purwanto, Y.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 1 (2012): February 2012
Publisher : Universitas Atma Jaya Yogyakarta

Penelitian ini bertujuan mempelajari pengetahuan tentang pemanfaatan hewan berpotensi, pelestarian lingkungan oleh masyarakat Tengger. Mempelajari interaksi antara masyarakat dan lingkungannya dan aspek praktek, persepsi serta representasinya. Metode penelitian digunakan survei exploratif meliputi inventarisasi jenis hewan di kandang, lingkungan rumah, wilayah konservasi hutan Taman Nasional Bromo Tengger Semeru (TN.BTS) meliputi nama lokal dan nama ilmiah. Metode dengan teknik ethnodirect, sampling meliputi wawancara langsung, semistruktural terhadap penduduk, pemangku adat, dukun serta pengumpulan informasi dengan pendekatan bersifat partisipasif (participatory ethnobotanical appraisal, PEA). Jenis hewan peliharaan mempunyai nilai ekonomi dapat dipergunakan sumber bahan pangan bagi masyarakat Tengger. Pengetahuan keanekaragaman satwa liar dan binatang yang dimanfaatkan masyarakat Tengger meliputi 110 jenis, hanya sekitar 6% saja yang sering digunakan dalam kehidupan sehari-hari rumah tangganya, diantaranya adalah untuk bahan pangan, ritual, obat-obatan, dan lain-lainnya.

KEANEKARAGAMAN DAN KELIMPAHAN ANGGREK EPIFIT DI KAKI GUNUNG LIANGPRAN, KALIMANTAN TIMUR Effendi, Surianto; Ariyanti, Nunik Sri; Chikmawati, Tatik
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Liangpran Mountain is located within the Heart of Borneo (HoB) Conservation Area which has high biodiversity. The exploration of orchids has been carried out in several locations in the HoB region and recorded a high diversity of wild orchid. This research was conducted to explore and inventory the orchids from the foothill of Mount Liangpran, and record the diversity and abundance of epiphytic orchids in the study sites. This research was carried out by making track of exploration along the Nuyung river estuary to the Tebing Lapah. The richness and abundance data were collected in three plot of 1000 x 20 m. The host tree characteristics and distribution of epiphytic orchid on the host tree were recorded. Fourteen species orchids were collected, and the most dominant are Coelogyne asperata and Agrostophyllum stipulatum with an abundance of 23.53% each. The epiphytic orchids in host trees are spread from the zone II to V based on the Johansson?s sections, in which zone IV is the most diverse. Nine species of the host trees associated with the collected orchids were identified and grouped into five families. The characters of host trees are 7?57 m height,12?112 cm diameters, and 2 types of bark surfaces (rough and intermediate).

PERSPEKTIF GENDER SUKU OSING DI BANYUWANGI DALAM PENILAIAN KEMANFAATAN TANAMAN Prasetyo, Budi; Chikmawati, Tatik; Walujo, Eko Baroto; Zuhud, Ervizal A.M
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3689

The different roles and responsibilities of male and female of the Osing tribe influence the knowledge of the use of plants. The aims of research is to analyze the values of the benefits of plants based on gender perception. The research used a qualitative and quantitative methods. Data collection for the value of plant species utilization were carried out using Pebble Distribution Method (PDM) and analysis used Index of Cultural Significance (ICS). The Osing community recognised 86 plant species that were grouped in to eight beneficial groups. The male in three villages have a better understanding of ethnobotanical knowledge and practice than female on crops as staple foods, medicines, fruits, and firewood, while understanding of ornamental plants are relatively the same for both male and female. Female have a broader and more detailed understanding on vegetables, plant species used as building construction, and traditional rituals. The high PDM value in eight beneficial groups was supported by the high value of each ICS.

Ethnobotany of MandailingTribe in Batang Gadis National Park Nasution, Aswarina; Chikmawati, Tatik; Walujo, Eko Baroto; Zuhud, Ervizal Amir Muhammad
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Â Batang Gadis National Park (BGNP) located in Bukit Barisan Mountains, Sumatera Utara. A Mandailing tribeÂ who lives around the BGNP, has the unique local knowledge, such as processing young stem of rattan (Calamus manan) into pakkat (traditional food) and use rimbang (Solanum torvum) to neutralize toxins. These local knowledge could be lost because it only inherited orally from generation to generation. This study was aimed to reveal ethnobotany knowledge of Mandailing Tribe. The study was conducted in November 2015 in four villages around the BGNP, Sibanggor Jae, Hutabaringin Julu, Pastap Jae, and Botung Villages. Data were collected by interviewing informants in each village as well as the field survey through two approaches, emic and etic. A total of 262 plant species is used by Mandailing Tribe for subsistence and commercial needs. The highest utilization is for foodÂ (106 species), followed by traditional medicines (81 species), firewood (29 species), building materials (35 species), and animal feed (25 species). People also used plant for household appliances, agricultural equipment, art materials, ropes and wrap, and pest control materials. Eme/rice (Oryza sativa) have the highest Index of Cultural Significance (ICS) values. The existence of this species is maintained for its local wisdom. Thus, involvement ofÂ localÂ community will give great contribution to manage and conserve the BGNP area.Â

Ecology of Rafflesia arnoldii (Rafflesiaceae) in Pandam Gadang West Sumatra Pranata, Syafroni; Sulistijorini, Sulistijorini; Chikmawati, Tatik
Journal of Tropical Life Science Vol 9, No 3 (2019)
Publisher : Journal of Tropical Life Science

Pandam Gadang has tropical forests which become the habitat of Rafflesia (Rafflesia arnoldii) which grows as a parasite of Tetrastigma (Vitaceae). Study on R. arnoldii is needed since its population continuously decrease as a result of habitat destruction of its host. The aim of the study was to determine habitat conditions, vegetation structure, and characteristic of the host species of Rafflesia; to identify environmental factors, and determine the Rafflesia habitat suitability in Pandam Gadang Koto Tinggi Village, West Sumatra. Analysis of vegetation in the habitat where Rafflesia grows using a circular plot. The number of individuals Rafflesia found was recorded and completed with observations of its morphology. Micro-climate measurements were carried out together with data collection in the field. Flower morphological analysis was descriptively presented. Environmental parameters were-processed with Canoco software 4.56 using Canonical Correspondence Analysis. Habitat suitability was analyzed using Maximum Entropy. The 17 Rafflesia individuals found on Pandam Gadang were R. arnoldii, known as "Cendawan Biriang", living parasitically on Tetrastigma leucostaphylum. The results of analysis of vegetation in the Rafflesia habitat showed that there were 250 individual plants consisting of 42 species and 21 genera. Seedlings most dominant was Lauraceae, belt were dominated by Moraceae, Meliaceae, and Euphorbiaceae. Trees were dominated by Euphorbiaceae, Moraceae, and Meliaceae. Existence and survival of R. arnoldii depended on soil pH 7-7.8, environmental humidity 83-91.8 % and distance from the nearest river 3-27.8 m.

Evaluation of Pulasan (Nephelium ramboutan-ake) Genetic Diversity in Bogor, West Java, Using Microsatellite Markers Puhili, Amelia Luisyane; Chikmawati, Tatik; Djuita, Nina Ratna
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.09

Pulasan (Nephelium ramboutan-ake (Labill.) Leenh) fruit is highly similar to rambutan rapiah (Nephelium lappaceum) fruit with ovate shape, sweet and sour fresh taste, but it has a thick rind. The diversity of pulasan is little informed including in Bogor. The objective of this study was to analyze the genetic diversity of pulasan from Bogor revealed by microsatellite marker. The DNA of 63 individuals from 10 populations of pulasan were extracted using CTAB method and amplified using two primer sets, LMLY6 (GA)9(CA)2(GA)4 dan LMLY12 (CT)11. DNA amplification product was visualized and arranged in a matrix of binary data then analyzed the value of the number of different alleles (Na), the number of effective alleles (Ne), Shannon information index (I), heterozygosity (He), and the percentage of polymorphism (PLP). The results of the analysis showed the highest genetic diversity was found in North Bogor (He=0.313). The genetic diversity within a population (61%) was higher than that among populations (39%). A dendrogram was constructed using the Unweighted Pair Group Method with arithmetic Mean (UPGMA). The similarity index ranged from 52 to 100% that means there are close relationships among individuals. Cluster analyses grouped some individuals originated from different locations in the same group. The levels of heterozygosity within a population was determined by the history of each individual in a population.

Co-Authors Adhy Widya Setiawan Adi Basukriadi ADI BASUKRIADI Afri Irawan Afri Irawan, Afri Ahmad Dwi Setyawan Akrom, Akrom Alex Hartana Alex Hartana Alex Hartana ALEX HARTANA Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Amelia Louisyane Puhili Amelia Luisyane Puhili Andayaningsih, Dwi Andi Madihah Manggabarani Anjar Tri Wibowo Anne Kusumawaty Anne Kusumawaty Arifin Surya Dwipa Irsyam Arifin Surya Dwipa Irsyam Asardi, Istiqoma Aswarina Nasution Awik Puji Dyah Nurhayati Badriah, Siti Nurul Billyardi Ramdhan Budi Prasetyo Budi Prasetyo BUDI SETIADI DARYONO Christyne SPLS Napitu Daniel Happy Putra Deby Arifiani Deby Arifiani, Deby Dede Setiadi Deden Girmansyah Dedy Darnaedi Dedy Darnaedi Dedy Darnaedi DEVI ALVITASARI Devi Alvitasari, Devi Devi Eka Lestari Dorly Dorly Dorly, Dwi Andayaningsih Dwi Putri Ramadhani Dwi Suci Setyaningsih Dwi Sunarti Puspitasari Dwi Sunarti Puspitasari, Dwi Sunarti Effendi, Surianto Eka Setiawan EKO BAROTO WALUJO Eko Baroto Walujo Eko Baroto Walujo Eko Baroto Waluyo Elizabeth A. Widjaja Elizabeth A. Widjaja Ervizal A.M Zuhud Ervizal Amir Muhammad Zuhud Ervizal AMZU Eva Kristinawati Putri Fadel Nugraha Furqon Al Muzakki Furqon Al Muzakki Gunawan Gunawan Gunawan Gunawan Haris Maulani HIMMAH RUSTIAMI Himmah Rustiami Himmah Rustiami Himmah Rustiami Himmah Rustiami, Himmah I Gusti Wayan Murjana Yasa I Putu Gede P. Damayanto I Putu Gede P. Damayanto I PUTU GEDE P. DAMAYANTO I PUTU GEDE P. DAMAYANTO, I PUTU GEDE P. Ibna Hayati IBNUL QAYIM Isna Arofatun Nikmah J PERRY GUSTAFSON Jati Batoro Jati Batoro Khoon Meng Wong Kuswata Kartawinata KUSWATA KARTAWINATA Lana Maulana Latifah, Vida Rahma Manggabarani, Andi Madihah Marlina Ardiyani maulana, lana Meitha, Karlia Meliza, Rezika Mentari Putri Pratami Mentari Putri Pratami Mien A. Rifai Mien A. Rifai Miftahudin . Miftahudin Miftahudin Miftahudin Miftahudin Muchlis . Mugi Mumpuni Muhammad Efendi Muhammad Efendi Muhammad Efendi, Muhammad Muhammad Rifqi Hariri Muhammad Rifqi Hariri Muzzazinah Muzzazinah Muzzazinah Muzzazinah Nasrianti Syam NASRIANTI SYAM, NASRIANTI SYAM, NASRIANTI Nasution, Aswarina Nasution, Aswarina Nina Ratna Djuita Novi Novitasari, Risti Nugroho, Giri NUNIK SRI ARIYANTI Nurul Aini Nurul Aini Oktavia Pradikaningrum, Novi Peniwidiyanti Peniwidiyanti Pieter Agusthinus Riupassa Pranata, Syafroni Priyanti Priyanti Priyanti Priyanti Priyanti, Pudji Widodo Puhili, Amelia Luisyane Puspita Dewi Sopyati Putro, Karso Suryo Qothrunnada - Sungkar Rambe, Saidah Putri Ratna Djuita, Nina Rezika Meliza Ridha Mahyuni Ridha Mahyuni Ridha Mahyuni Ridha Mahyuni Risti Novitasari Rizki, Fitri Sri Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Safniyeti Safniyeti Safniyeti Safniyeti, Safniyeti Sartika, Dian Siti Halimatussadiah Sobir Sobir Sobir Sobir Sobir, Sri Endarti Rahayu Sri Endarti Rahayu, Sri Endarti Suharsono Suharsono Suharsono, Sulistijorini Sulistijorini Syadwina Hamama Dalimunthe Syadwina Hamama Dalimunthe Syafroni Pranata Tira Tahnia Titien Ngatinem Praptosuwiryo Titien Ngatinem Praptosuwiryo Titin Ngatinem Praptosuwiryo Tnunay, Ite Morina Yostianti Wakhidah, Anisatu Z. WISNU HANDOYO ARDI WISNU HANDOYO ARDI, WISNU HANDOYO Y. Purwanto Y. Purwanto Yayan Wahyu Candra Kusuma Yohanes Purwanto Yusuf, Adib Fakhruddin Zulhendra zulhendra Zumaidar Zumaidar

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