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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Bios Logos BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Wacana, Jurnal Sosial dan Humaniora Proceeding International Conference on Global Resource Conservation Journal of Mathematical and Fundamental Sciences Jurnal Sumberdaya Hayati Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology REINWARDTIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Forest and Society Floribunda Proceeding Biology Education Conference Makara Journal of Science Reinwardtia Jurnal Biosilampari: Jurnal Biologi

TATIK CHIKMAWATI

Bogor Agricultural University

Author-ID : 247116

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Growth and Development of Apogamous Adiantum lunulatum Burm. f. Gametophyte from Dry and Humid Areas in Java Island Novitasari, Risti; Chikmawati, Tatik; Sulistijorini, Sulistijorini
Journal of Tropical Life Science Vol 7, No 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.15

Adiantum lunulatum Burm. f. has been widely used in aesthetic needs and medical fields. Detail information about the life cycle of A. lunulatum is still unknown, particularly about its gametophyte generation. Present work aimed to study and compare the growth and development of the gametophytes of A. lunulatum from dry and humid areas. Research method consists of two stages: (1) Spores were collected from two locations, Pasuruan and Bogor and (2) Spores were planted and observed the growth and development of gametophyte. The result showed that natural habitat of the plant in various dry and humid areas, affecting the rate of growth and development rates of A. lunulatum gametophyte. The gametophyte from the dry area showed faster rate of growth and development than that of humid areas. The spores collected from dry area need 9 weeks for germination, growth, and development while the spores of humid area took 22 weeks.

Gametophyte Morphology and Development of Six Species of Pteris (Pteridaceae) from Java Island Indonesia Puspitasari, Dwi Sunarti; Chikmawati, Tatik; Praptosuwiryo, Titien Ngatinem
Journal of Tropical Life Science Vol 5, No 2 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.02.08

The morphology of sporophyte, the type of reproduction, and cytology of Pteris had been reported, while the gametophyte morphology of Pteris in Java island has not been studied yet. The objective of this study was to describe the gametophyte morphology and development of P. biaurita, P. ensiformis,Â P.exelsa, P.longipinnula, P. tripartita, andÂ Â P. vittata in Java island. Spores were obtained from fertile leaves of Pteris plants originated from several locations in Java island. The number of spores per sporangium were counted from fresh fertile leaves with mature sporangia. As much as 0.002 g spores was sown in transparent box with sterile medium contain of vermiculite, spaghnum moss, and perlite with ratio 2:2:1. The gametophyte development of each species was observed under a microscope every 7 days. The spores of P. ensiformis were germinated faster, 10 days after sowing, while the spores of P.longipinnula were germinated slower, 18 days after sowing.Â The pattern of spore germination is Vittaria-type. The development of gametophyte is Ceratopteris-type in common, but in a few cases is the Adiantum-type. The gametophyte development of observed Pteris spesies are varied in six characters including the number of filament cell, germinated time, formation time of notch and gametangia, margin shape, and development type.

Anatomical and Cytological Features of Spathoglottis plicata from Java Island Chikmawati, Tatik
Journal of Tropical Life Science Vol 3, No 2 (2013)
Publisher : Journal of Tropical Life Science

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Spathoglottis plicata is a ground orchid that displays large variation in flower features. In spite of large variation on morphological characters, its anatomical and cytological characters are not well known. This paper described the leaf anatomical and chromosomal features of S. plicata. Leaf anatomy was observed from both transverse and paradermal sections of leaves. Chromosome numbers were observed from root tip cells. Epidermis of all S. plicata variants comprised of two cell shapes, the rectangle cells located above veins, while the polygonal one located in between veins. Stomatal type was brachitetracyclic, which were only found on lower epidermis. Leaf transversal section comprised of one layer of upper and lower epidermis, spongy mesophyll, and vascular tissue. The number of leaf hair varied depended on the original habitat and altitute. All variants of S. plicata had same chromosome number (2n=40).

INDIGOFERA: â€œKINI DAN NANTIâ€ Muzzazinah, Muzzazinah; Chikmawati, Tatik; Rifai, Mien A.; Ariyanti, Nunik Sri
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract- A few people know Indigofera, but as a admirer of batik and tenun, these plant gives hope for the preservation of batik. Indigofera has a variety of benefits , but more commonly known as blue dye in batik and tenun. The problems of restore the glory of Indigofera from Indonesia who had experienced the triumph can be divided into three principal. There are the increasing scarcity of Indigofera plant, the lack of willingness of the community batik for the cultivation, the process of indigo dyeing longer and complicated, and the results of staining with the natural color is not uniform. The problem by the business presence of more efficient synthetic dyes,Â the price of synthetic dyes is cheaper. While research on Indigofera dye is still limited and stagnate, this is a problem should be a concern Botanist .Â Â Keywords: Indigofera, batik, tenun, natural dye

Pertumbuhan dan analisis kualitatif tanin, saponin dan flavonoid dari Selaginella plana, S. willdenovii and S. mayeri pada tiga naungan berbeda (The growth and qualitative analysis of tannin, saponin and flavonoid in Selaginella plana, S. willdenovii an Chikmawati, Tatik; Sopyati, Puspita Dewi; Miftahudin, Miftahudin
JURNAL BIOS LOGOS Vol 3, No 1 (2013): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.3.1.2013.14503

Abstrak Selaginella yang banyak dijumpai melimpah di Indonesia sangatÂ berpotensi sebagai sumber antioksidan alami. Kadar bahan bioaktif dalam tumbuhanÂ sangat tergantung pada lingkungan tumbuhnya, namun penelitian aspek budidaya dari Selaginella di Indonesia yang berkaitan dengan bahan bioaktif belum banyak dilakukan.Â Penelitian awal tentang aspek budidaya Selaginella telah dilakukan khususnya untuk mengetahui naungan yang tepat bagi pertumbuhannya serta pengaruhnya terhadap kandungan bahan bioaktif. Penelitian ini menggunakan rancangan petak terpisah dengan naungan sebagai petak utama dan jenis Selaginella sebagai anak petak. S. plana, S. willdenovii dan S. mayeri diberi perlakuan naungan dengan paranet 40%, 65%, 80%, dan tanpa naungan. Uji kandungan tanin, saponin, dan flavonoid secara kualitatif diketahui dengan melakukan ekstraksi dengan alkohol. Pertambahan cabang total, pertambahan bobot basah total, bobot basah, dan bobot kering biomassa dipengaruhi secara nyata oleh naungan dan jenis Selaginella. Masing-masing jenis Selaginella memerlukan intensitas cahaya yang berbeda untuk mendukung pertumbuhannya. Semua jenis yang diuji juga mengandung kadar tanin, saponin, dan flavonoid berbeda secara kualitatif. Kata kunci:Â Selaginella, naungan, tanin, saponin, flavonoid Abstract Selaginella found abandonly in Indonesia is very potensial as the source of natural antioxidant. The amount of bioactive material of Selaginella depends on the environment, but research on the amount of bioactive material correlated to the cultivated aspect has not been done in Indonesia. The preeliminary research of the cultivation of Selaginella wasÂ investigated especially to know the right shading for the best growth of Selaginella. The right shading may also affect bioactive content of Selaginella. This research used split plot design with level of shading as main plot and species as sub plot. S. plana, S. willdenovii, and S. mayeri were grown under paranet with 40%, 65%, 80% shading, and non shading treatments. Bioactive compound was extracted using alcohol 70% and analyzed for tannin, saponin, and flavonoid contents. Results indicated that level of shading and species affected total branch increase, total fresh weight increase, fresh and dry weight of biomass.Â Each species required different light intensity to support its growth. All species in the experiment contained different qualitative level of tannin, saponin, and flavonoid. Keywords: Selaginella, shade, tanin, saponin, flavonoid

Rye (Secale cereale L.) and Wheat (Triticum aestivum L.) Simple Sequence Repeat Variation within Secale spp. (Poaceae) TATIK CHIKMAWATI; . MIFTAHUDIN; J PERRY GUSTAFSON
HAYATI Journal of Biosciences Vol. 20 No. 4 (2013): December 2013
Publisher : Bogor Agricultural University, Indonesia

Transferability of 25 rye and 20 wheat simple sequence repeat (SSR) markers were examined across Secale spp. to elucidate their genetic relationships. One hundred percent of rye and 75% of wheat SSR markers could be amplified in weedy and wild forms of Secale. However, only 84% of rye SSR markers were amplified in S. sylvestre indicating that this species had the furthest relation to the cultivated rye. The PCR products resulting from rye SSR tended to have similar size as the original size, while wheat SSRs tended to have a shorter size than that of the original size. Polymorphism in the allelic number and size was found in 68% of the rye and 40% of the wheat SSR markers. Several alleles were unique for the annual and perennial rye taxa, which were counted for 22 and 15%, respectively. Some SSR markers also produced specific alleles that could be used to identify certain taxa. The genetic relationships within the genus Secale based on SSR markers were discussed.

THE ANATOMICAL CHARACTERS OF Ampelocissus (Vitaceae) IN SUMATRA Syadwina Hamama Dalimunthe; Tatik Chikmawati; Elizabeth A. Widjaja
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

The revision of Ampelocissus was performed by observing the anatomical character of Ampelocissus leaves. The leaves were collected from 33 collection numbers of Herbarium Bogoriense (BO) and four collection numbers from Sumatra exploration. The purpose of this study is to update the information of diversity and to support species concept delimitation of Ampelocissus based on the anatomical study, especially on the sterile plant. Anatomical characters were observed on nine species and one variety of Sumatran Ampelocissus. Ampelocissus species is varied by the anatomical characters, i.e.: shape of the anticlinal wall of abaxial and adaxial epidermal cell, number of palisade layers, upper epidermal cell thickness, leaf thickness, presence of papillae on stomata neighboring cell, type, and shape of calcium oxalate crystals, also stomata position. The cluster analysis of Ampelocissus in Sumatra based on 16 anatomical characters showed the coefficient similarity in the range of 0.48 - 0.81. The research showed that the leaf anatomical characters can be used as additional characters to distinguish the species of Ampelocissus. Keywords: calcium oxalate crystals, diversity, papillae, similarity coefficient, species concept ABSTRAK Ciri Anatomi Ampelocissus (Vitaceae) di Sumatera Revisi Ampelocissus dilakukan dengan mengamati ciri anatomi daun dari marga Ampelocissus. Sebanyak 33 nomor koleksi Herbarium Bogoriense (BO) dan empat nomor koleksi hasil eksplorasi di Sumatera digunakan. Penelitian ini bertujuan untuk memperbarui informasi mengenai keanekaragaman dan mendukung konsep jenis Ampelocissus berdasarkan ciri anatomi, terutama pada tumbuhan steril. Studi anatomi dilakukan pada sembilan jenis dan satu varietas Ampelocissus di Sumatera. Jenis-jenis Ampelocissus di Sumatera bervariasi pada ciri anatomi yaitu bentuk dinding antilkinal sel epidermis pada bagian abaksial dan adaksial, jumlah lapisan jaringan tiang, ketebalan sel epidermis atas, ketebalan daun, kehadiran papila, jenis dan bentuk kristal kalsium oksalat, serta posisi stomata terhadap sel epidermis pada bagian abaksial daun. Analisis pengelompokan Ampelocissus di Sumatera menggunakan 16 ciri antomi dan menghasilkan fenogram dengan koefisien kemiripan 0,48 - 0,81. Ciri anatomi daun dapat digunakan sebagai ciri tambahan yang memiliki nilai taksonomi dalam membedakan jenis-jenis Ampelocissus di Sumatera.

Perspektif Kultural Pengelolaan Lingkungan pada Masyarakat Adat Cikondang Kabupaten Bandung Jawa Barat Billyardi Ramdhan; Tatik Chikmawati; Eko Baroto Waluyo
Jurnal Sumberdaya Hayati Vol. 1 No. 1 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.1.7-14

Peoples of Traditional Cikondang Village, located in the village of Lamajang, Pangalengan, Bandung City West Java have a very close relation to the environment along with its natural resources. This relation is expressed in the form of procedures and rules on the management and utilization of landscape units as well as the diversity of plants as a form of adaptation to the natural environment. This research was conducted through ethnographic method for photographing people’s lives and ethnobotany to know the biological resources that are important to Cikondang’s people’s daily lives. Descriptive and analytical methods are used through interviews with 87 respondents and 4 key informants. Results of research on landscape units that were identified by the community, indicates that in terms of the character, function, and its management there are 9 units of landscape that is both natural landscape and which has been modified by Cikondang community activities. Each unit of the landscape is characterized by vegetation cover according to the type of value in order for daily life. Meanwhile, cultural factors, economic, and political could be a trigger to the landscape transformation of one type of landscape into other landscapes.

Pertumbuhan dan Kandungan Bahan Bioaktif Selaginella plana dan Selaginellla willdenovii pada Beberapa Media Tanam Miftahudin .; Dwi Suci Setyaningsih; Tatik Chikmawati
Jurnal Sumberdaya Hayati Vol. 1 No. 1 (2015)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.1.1.1-6

Selaginella, a genus of Pteridophyte, is known as an herb that contains of several bioactive compounds. The growth and bioactive compounds of plant were affected by plant environment, such as media types. The objective of the research was to obtain an appropriate plant media for growing S. plana dan S. willdenovii, and its effect on the content of bioactive compounds. The experiment consisted of two factors, plant growth media and the species of Selaginella. The first factor consisted of eight plant growth media, i.e.: soil, rice husk, rice husk : soil = 1:1 (v/v), burned rice husk, soil : burned rice huks = 1:1 (v/v), burned rice husk : rice husk = 1:1 (v/v), burned rice husk : rice husk = 3:1 (v/v), and burned rice husk : rice husk = 1:3 (v/v). The second factor was two spesies of Selaginella, i.e.: S. plana and S. willdenovii. Ethanol extract of Selaginella was qualitatively analyzed for flavonoid, tannin, and saponin content. The result showed that both fresh and dry weights of Selaginella were influenced by the type of plant media, Selaginella species, and their interactions. S. plana and S. willdenovii grew best on medium containing soil: burned rice = 1:1 (v/v). The highest flavonoid content of S. plana and S. willdenovii was also obtained from the plant grown on the same media. Comparing to the bioactive compound content of natural plant, saponin content of S. plana and S. willdenovii was increased by the treatment of plant media. The treatment also increased tannin content of S. plana, but not of S. willdenovii.

Variasi Struktur Anatomi Daun Beberapa Jenis Alpinia Roxb. di Malesia Eka Setiawan; Miftahudin Miftahudin; Marlina Ardiyani; Tatik Chikmawati
Al-Kauniyah: Jurnal Biologi Vol 14, No 2 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i2.17446

AbstrakKetidaksesuaian sistem klasifikasi Alpinia berdasarkan ciri morfologi dengan ciri molekuler menyebabkan sistem klasifikasi pada marga ini masih bermasalah. Data tambahan dari anatomi Alpinia diperlukan untuk mendukung sistem klasifikasi pada marga ini. Sayatan paradermal adaksial dan abaksial dari daun tujuh jenis Alpinia diamati. Sampel diambil dari koleksi Kebun Raya Bogor (KRB) yang mewakili jenis A. galanga, A. mutica, A. padacanca, A. macrocrista, A. malaccensis, A. pusilla, dan A. ligulata. Sebelas ciri Alpinia diamati seperti; ukuran stomata, jumlah stomata, indeks stomata, kerapatan stomata, tipe stomata, ukuran sel tetangga, jumlah sel tetangga, bentuk sel tetangga, ukuran epidermis, jumlah epidermis, dan bentuk epidermis. Hasil pengamatan menunjukkan bahwa beberapa ciri dapat digunakan untuk membedakan jenis Alpinia, seperti bentuk dan ukuran epidermis; ukuran, kerapatan dan indeks stomata; serta ukuran sel tetangga. Sementara ciri lainnya, seperti tipe stomata dan bentuk sel tetangga pada semua jenis Alpinia memiliki bentuk yang sama sehingga tidak dapat digunakan untuk membedakan jenis Alpinia.Abstract The incompatibility of Alpinia classification system based on morphological and molecular character causes classification system of this genus is still problematic. Additional data from Alpinia anatomy are needed to support the classification system in this genus. Adaxial and abaxial sides paradermal incisions of seven Alpinia species were observed. The samples were taken from the Bogor Botanical Gardens (KRB) collection representing A. galanga, A. mutica, A. padacanca, A. macrocrista, A. malaccensis, A. pusilla, and A. ligulata. Eleven Alpinia characteristics were observed; stomatal size, number of stomata, stomatal index, stomatal density, stomatal type, neighboring cells size, number of neighboring cells, neighboring cell shape, epidermal cells size, number of epidermal cells, and epidermal cells shape. The results showed that several characteristics can be used to distinguish Alpinia species, such as the shape and size of epidermal cells; the size, density and index of stomata; and the size of neighboring cells. Several other characteristics, such as the type of stomata and shape of neighboring cells in all species of Alpinia have the same shape so the characters cannot be used to distinguish Alpinia species.

Co-Authors Adhy Widya Setiawan Adi Basukriadi ADI BASUKRIADI Afri Irawan Afri Irawan, Afri Ahmad Dwi Setyawan Akrom, Akrom Alex Hartana ALEX HARTANA Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Amelia Louisyane Puhili Amelia Luisyane Puhili Andayaningsih, Dwi Andi Madihah Manggabarani Anjar Tri Wibowo Anne Kusumawaty Anne Kusumawaty Arifin Surya Dwipa Irsyam Arifin Surya Dwipa Irsyam Asardi, Istiqoma Aswarina Nasution Awik Puji Dyah Nurhayati Badriah, Siti Nurul Billyardi Ramdhan Budi Prasetyo Budi Prasetyo BUDI SETIADI DARYONO Christyne SPLS Napitu Daniel Happy Putra Deby Arifiani Deby Arifiani, Deby Dede Setiadi Deden Girmansyah Dedy Darnaedi Dedy Darnaedi Dedy Darnaedi DEVI ALVITASARI Devi Alvitasari, Devi Devi Eka Lestari Dorly Dorly Dorly, Dwi Andayaningsih Dwi Putri Ramadhani Dwi Suci Setyaningsih Dwi Sunarti Puspitasari Dwi Sunarti Puspitasari, Dwi Sunarti Effendi, Surianto Eka Setiawan EKO BAROTO WALUJO Eko Baroto Walujo Eko Baroto Walujo Eko Baroto Waluyo Elizabeth A. Widjaja Elizabeth A. Widjaja Ervizal A.M Zuhud Ervizal Amir Muhammad Zuhud Ervizal AMZU Eva Kristinawati Putri Fadel Nugraha Furqon Al Muzakki Furqon Al Muzakki Gunawan Gunawan Gunawan Gunawan Haris Maulani Himmah Rustiami HIMMAH RUSTIAMI Himmah Rustiami Himmah Rustiami Himmah Rustiami, Himmah I Gusti Wayan Murjana Yasa I PUTU GEDE P. DAMAYANTO I Putu Gede P. Damayanto I Putu Gede P. Damayanto I PUTU GEDE P. DAMAYANTO, I PUTU GEDE P. Ibna Hayati IBNUL QAYIM Isna Arofatun Nikmah J PERRY GUSTAFSON Jati Batoro Jati Batoro Junaidi, Muhamad Khoon Meng Wong Kuswata Kartawinata KUSWATA KARTAWINATA Lana Maulana Latifah, Vida Rahma Manggabarani, Andi Madihah Marlina Ardiyani maulana, lana Meitha, Karlia Meliza, Rezika Mentari Putri Pratami Mentari Putri Pratami Mien A. Rifai Mien A. Rifai Miftahudin . Miftahudin Miftahudin Miftahudin Miftahudin Muchlis . Mugi Mumpuni Muhammad Efendi Muhammad Efendi Muhammad Efendi, Muhammad Muhammad Rifqi Hariri Muhammad Rifqi Hariri Muzzazinah Muzzazinah Muzzazinah Muzzazinah Nasrianti Syam NASRIANTI SYAM, NASRIANTI SYAM, NASRIANTI Nasution, Aswarina Nasution, Aswarina Nina Ratna Djuita Novi Novitasari, Risti Nugroho, Giri NUNIK SRI ARIYANTI Nurul Aini Nurul Aini Oktavia Pradikaningrum, Novi Peniwidiyanti Peniwidiyanti Pieter Agusthinus Riupassa Pranata, Syafroni Priyanti Priyanti Priyanti Priyanti Priyanti, Pudji Widodo Puhili, Amelia Luisyane Puspita Dewi Sopyati Putro, Karso Suryo Qothrunnada - Sungkar Rambe, Saidah Putri Ratna Djuita, Nina Rezika Meliza Ridha Mahyuni Ridha Mahyuni Ridha Mahyuni Ridha Mahyuni Risti Novitasari Rizki, Fitri Sri Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Safniyeti Safniyeti Safniyeti Safniyeti, Safniyeti Sartika, Dian Siti Halimatussadiah Sobir Sobir Sobir Sobir Sobir, Sri Endarti Rahayu Sri Endarti Rahayu, Sri Endarti Suharsono Suharsono Suharsono, Sulistijorini Sulistijorini Syadwina Hamama Dalimunthe Syadwina Hamama Dalimunthe Syafroni Pranata Tira Tahnia Titien Ngatinem Praptosuwiryo Titien Ngatinem Praptosuwiryo Titin Ngatinem Praptosuwiryo Tnunay, Ite Morina Yostianti Tri Atmowidi Wakhidah, Anisatu Z. WISNU HANDOYO ARDI WISNU HANDOYO ARDI, WISNU HANDOYO Y. Purwanto Y. Purwanto Yayan Wahyu Candra Kusuma Yohanes Purwanto Yusuf, Adib Fakhruddin Zulhendra zulhendra Zumaidar Zumaidar

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