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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Bios Logos BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Wacana, Jurnal Sosial dan Humaniora Proceeding International Conference on Global Resource Conservation Journal of Mathematical and Fundamental Sciences Jurnal Sumberdaya Hayati Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology REINWARDTIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Forest and Society Floribunda Proceeding Biology Education Conference Makara Journal of Science Reinwardtia Jurnal Biosilampari: Jurnal Biologi
TATIK CHIKMAWATI
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Evaluation of Pulasan (Nephelium ramboutan-ake) Genetic Diversity in Bogor, West Java, Using Microsatellite Markers Amelia Luisyane Puhili; Tatik Chikmawati; Nina Ratna Djuita
Journal of Tropical Life Science Vol. 6 No. 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.09

Abstract

Pulasan (Nephelium ramboutan-ake (Labill.) Leenh) fruit is highly similar to rambutan rapiah (Nephelium lappaceum) fruit with ovate shape, sweet and sour fresh taste, but it has a thick rind. The diversity of pulasan is little informed including in Bogor. The objective of this study was to analyze the genetic diversity of pulasan from Bogor revealed by microsatellite marker. The DNA of 63 individuals from 10 populations of pulasan were extracted using CTAB method and amplified using two primer sets, LMLY6 (GA)9(CA)2(GA)4 dan LMLY12 (CT)11. DNA amplification product was visualized and arranged in a matrix of binary data then analyzed the value of the number of different alleles (Na), the number of effective alleles (Ne), Shannon information index (I), heterozygosity (He), and the percentage of polymorphism (PLP). The results of the analysis showed the highest genetic diversity was found in North Bogor (He=0.313). The genetic diversity within a population (61%) was higher than that among populations (39%). A dendrogram was constructed using the Unweighted Pair Group Method with arithmetic Mean (UPGMA). The similarity index ranged from 52 to 100% that means there are close relationships among individuals. Cluster analyses grouped some individuals originated from different locations in the same group. The levels of heterozygosity within a population was determined by the history of each individual in a population.
Growth and Development of Apogamous Adiantum lunulatum Burm. f. Gametophyte from Dry and Humid Areas in Java Island Risti Novitasari; Tatik Chikmawati; Sulistijorini Sulistijorini
Journal of Tropical Life Science Vol. 7 No. 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.15

Abstract

Adiantum lunulatum Burm. f. has been widely used in aesthetic needs and medical fields. Detail information about the life cycle of A. lunulatum is still unknown, particularly about its gametophyte generation. Present work aimed to study and compare the growth and development of the gametophytes of A. lunulatum from dry and humid areas. Research method consists of two stages: (1) Spores were collected from two locations, Pasuruan and Bogor and (2) Spores were planted and observed the growth and development of gametophyte. The result showed that natural habitat of the plant in various dry and humid areas, affecting the rate of growth and development rates of A. lunulatum gametophyte. The gametophyte from the dry area showed faster rate of growth and development than that of humid areas. The spores collected from dry area need 9 weeks for germination, growth, and development while the spores of humid area took 22 weeks.
Ethnobotany of MandailingTribe in Batang Gadis National Park Aswarina Nasution; Tatik Chikmawati; Eko Baroto Walujo; Ervizal Amir Muhammad Zuhud
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.09

Abstract

 Batang Gadis National Park (BGNP) located in Bukit Barisan Mountains, Sumatera Utara. A Mandailing tribe  who lives around the BGNP, has the unique local knowledge, such as processing young stem of rattan (Calamus manan) into pakkat (traditional food) and use rimbang (Solanum torvum) to neutralize toxins. These local knowledge could be lost because it only inherited orally from generation to generation. This study was aimed to reveal ethnobotany knowledge of Mandailing Tribe. The study was conducted in November 2015 in four villages around the BGNP, Sibanggor Jae, Hutabaringin Julu, Pastap Jae, and Botung Villages. Data were collected by interviewing informants in each village as well as the field survey through two approaches, emic and etic. A total of 262 plant species is used by Mandailing Tribe for subsistence and commercial needs. The highest utilization is for food  (106 species), followed by traditional medicines (81 species), firewood (29 species), building materials (35 species), and animal feed (25 species). People also used plant for household appliances, agricultural equipment, art materials, ropes and wrap, and pest control materials. Eme/rice (Oryza sativa) have the highest Index of Cultural Significance (ICS) values. The existence of this species is maintained for its local wisdom. Thus, involvement of  local  community will give great contribution to manage and conserve the BGNP area. 
Ecology of Rafflesia arnoldii (Rafflesiaceae) in Pandam Gadang West Sumatra Syafroni Pranata; Sulistijorini Sulistijorini; Tatik Chikmawati
Journal of Tropical Life Science Vol. 9 No. 3 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.03.02

Abstract

Pandam Gadang has tropical forests which become the habitat of Rafflesia (Rafflesia arnoldii) which grows as a parasite of Tetrastigma (Vitaceae). Study on R. arnoldii is needed since its population continuously decrease as a result of habitat destruction of its host. The aim of the study was to determine habitat conditions, vegetation structure, and characteristic of the host species of Rafflesia; to identify environmental factors, and determine the Rafflesia habitat suitability in Pandam Gadang Koto Tinggi Village, West Sumatra. Analysis of vegetation in the habitat where Rafflesia grows using a circular plot. The number of individuals Rafflesia found was recorded and completed with observations of its morphology. Micro-climate measurements were carried out together with data collection in the field. Flower morphological analysis was descriptively presented. Environmental parameters were-processed with Canoco software 4.56 using Canonical Correspondence Analysis. Habitat suitability was analyzed using Maximum Entropy. The 17 Rafflesia individuals found on Pandam Gadang were R. arnoldii, known as "Cendawan Biriang", living parasitically on Tetrastigma leucostaphylum. The results of analysis of vegetation in the Rafflesia habitat showed that there were 250 individual plants consisting of 42 species and 21 genera. Seedlings most dominant was Lauraceae, belt were dominated by Moraceae, Meliaceae, and Euphorbiaceae. Trees were dominated by Euphorbiaceae, Moraceae, and Meliaceae. Existence and survival of R. arnoldii depended on soil pH 7-7.8, environmental humidity 83-91.8 % and distance from the nearest river 3-27.8 m.
Endemic Bamboo (Poaceae, Bambusoideae) of the Lesser Sunda Islands I Putu Gede P. Damayanto; Himmah Rustiami; Miftahudin Miftahudin; Tatik Chikmawati
Jurnal Biodjati Vol 8, No 1 (2023): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i1.25015

Abstract

More than 40 species of bamboo were known from the Lesser Sunda Islands (LSI), including several endemic species. Endemic species are often highly specialized, found in limited geographical areas, and vulnerable to extinction if their habitat is destroyed. This study aimed to provide an account of endemic bamboo species on the LSI. Fieldwork involved collecting samples of bamboo in the LSI. Herbarium specimens of bamboo deposited in the Herbarium Bogoriense (BO), collected from the LSI were examined, and endemic LSI species were recorded. The conservation status followed the International Union for Conservation of Nature (IUCN) red list categories and criteria. The conservation status of unassessed species was evaluated using IUCN red list categories and criteria. A spatial analysis was also conducted. Data were analyzed and presented descriptively. There are 10 endemic bamboo species in the LSI, namely Bambusa ooh Widjaja & Astuti from Bali (conservation status: endangered), Chloothamnus reholttumianus (Soenarko) Widjaja from Sumba (vulnerable), C. schmutzii (S.Dransf.) Widjaja from Sumbawa and Flores (near threatened), Dinochloa kostermansiana S.Dransf. from Sumba and Flores (near threatened), D. sepang Widjaja & Astuti from Bali (vulnerable), Fimbribambusa rifaiana Widjaja from Alor (near threatened), Gigantochloa aya Widjaja & Astuti from Bali (vulnerable), G. baliana Widjaja & Astuti from Bali (vulnerable), G. taluh Widjaja & Astuti from Bali (vulnerable), and Schizostachyum purpureum Damayanto & Widjaja from Sumba (data deficient). They can be found at an altitude of 20–1700 m in various habitats, including forests, bamboo forests, villages, botanical gardens, along a road, slopes, valleys, riverbanks, and around a lake.
THE TYPIFICATION OF GIGANTOCHLOA TALUH WIDJAJA & ASTUTI (POACEAE, BAMBUSOIDEAE) I Putu Gede P. Damayanto; Himmah Rustiami; Miftahudin Miftahudin; Tatik Chikmawati
REINWARDTIA Vol 22, No 1 (2023): Volume 22, No 1 (2023): Reinwardtia
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2023.4567

Abstract

The name of Gigantochloa taluh Widjaja & Astuti (Poaceae, Bambusoideae) was invalid because of failure to meet the requirements of the International Code of Nomenclature for algae, fungi, and plants, that there was no herbarium location of type specimens was mentioned. A typification was provided here to address this issue.
Diversity and Distribution Patterns of Epiphytic Orchid in the Nirmala Tea Plantation, Bogor West Java Nurul Aini; Tatik Chikmawati; Sulistijorini Sulistijorini; Nina Ratna Djuita; Nunik Sri Ariyanti
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 1 (2022): April 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i1.35076

Abstract

Tea plantations can provide microclimate conditions for many epiphytic species, including orchids, but information on their diversity is not yet available. Epiphytic orchid diversity study has been carried out at the Nirmala Tea Plantation, Bogor, West Java. The main objective of this study was to reveal the diversity of epiphytic orchids in the Nirmala Tea Plantation. The data were obtained by vegetation analysis using the purposive sampling method by making 54 plots sized 5 x 5m2 at two different altitudes, 1050 and 1500 masl. The quantitative data were analyzed by calculating the critical value and morisita indices. The correlation between epiphytic orchid and microclimate variables was processed using Spearman bivariate correlation, performed using Canonical Correspondence Analysis. A total of 15 epiphytic orchids were found at an altitude of 1050 m asl and 10 species of epiphytic orchids at an altitude of 1500 m asl. Seven species, Agrostophyllum longifolium Rchb.f., Appendicula reflexa Blume, Bulbophyllum sp.2, Dendrochilum sp., Eria sp.1, Flickingeria sp, Pholidota carnea Lindl, were found at both altitudes. The diversity of epiphytic orchids is affected by altitude, light intensity, and wind speed. The distribution pattern of epiphytic orchids in Nirmala Tea Plantation is clumped. The results of this study report the diversity of orchids grown in tea plantations for the first time. This information is helpful for the cultivation and conservation of orchid diversity in the future.
Characterization of Rambutan Cultivars (Nephelium lappaceum) Based on Leaf Morphological and Genetic Markers Andi Madihah Manggabarani; Tatik Chikmawati; Alex Hartana
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12221

Abstract

Rambutan (Nephelium lappaceum) is an economically important plant which is native to Indonesia and Malaysia. The diversity of rambutan in Indonesia is abundance especially in Kalimantan where the wild relatives still grow naturally. Rambutan cultivars are usually differed from each other based on fruit morphological characters. However, rambutan tree begins to fruiting for the first time in 3-4 years. Therefore, another character is needed to characterize each cultivar in a short period. The objectives of this study were to distinguish rambutan cultivars using leaf morphological and Inter-Simple Sequence Repeat (ISSR). As many as 30 rambutan cultivars collected from Cipaku Orchard and Mekar Sari Park were observed for their morphological and ISSR characters. Six characters of were surveyed for leaf morphological character. For the genetic character, 6 out of 31 ISSR primers were assessed which resulted in 58 polymorphic bands (87%). As a result, leaf morphological characters overlapped among cultivars causing difficulties distinguishing each cultivar. ISSR marker, three major clusters have been identified according to UPGMA method. Index similarity among rambutan accessions from ISSR data ranged from 48-93%. As a conclusion, ISSR marker could be potentially applied rambutan cultivars characterization.
Variasi Sisik Tumbuhan Paku Terestrial di Kampus IPB, Dramaga, Bogor : Variations of Terrestrial Scale Fern on The Campus of IPB University, Dramaga, Bogor Siti Halimatussadiah; Nina Ratna Djuita; Tatik Chikmawati Chikmawati
Jurnal Sumberdaya Hayati Vol. 9 No. 3 (2023): 2023
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.3.109-118

Abstract

IPB Dramaga campus has high biodiversity, one of which is terrestrial ferns. Ferns have scales or hairs that cover the rhizome, petiole and leaf blade. The characteristic of scales can be used in the identification of fern species. The aim of this study was to determine the morphological diversity of terrestrial fern scales collected from the IPB Dramaga campus. The fern scales was observed under a light microscope connected to an indomicro and the density was calculated. The rhizome and petiole scales found in the form of lanceolate, ovate, subulate, linear, and reniform. The tip of the scales varies from thick, and needle-like, thin, and thick. The edges of the scales vary, namely threaded, flat, or banded. The attachments of the base of the scales found were, peltate. pseudopeltate, and basifix. The color of the scales varies from transparent, yellow, light brown, fawn, and dark brown. The scale density ranged from 0.6 to 21.3/mm2. The Pteridaceae have more characteristics in common between their genera than the other two family, but these similarities cannot be used for taxonomic identification and classification, because they are not specific for each genus.
Phytochemical Composition of Selaginella spp. from Java Island Indonesia Chikmawati, Tatik; Setyawan, Ahmad Dwi; Miftahudin, Miftahudin
Makara Journal of Science Vol. 16, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

For a long time, people in West Java, Indonesia have used Selaginella as a traditional cure for several ailments including fever, minor wounds, broken bones, women’s health disorders or postnatal bleeding. However, information on the active compounds of the plant from Java Island has not been fully expounded. The objective of the research was to reveal the diversity of bioactive compounds and amentoflavone content of Selaginella from Java Island in order to optimize the use of this plant as a medicinal plant. Selaginella plants collected from 29 locations in Java were extracted and subjected to colorimetric and thin layer chromatography test to qualitatively analyze the bioactive compounds of alkaloid, phenol, and steroid. In addition, high performance liquid chromatography was performed to analyze the amentofalvone content of Selaginella extract. The research result showed that all the examined Selaginella species contained alkaloid, flavonoid, saponin, tannin, and steroid, but they did not contain hydroquinone. Seven of them contained the biflavonoid type of amentoflavone. The highest amentoflavone concentration, 6.87 ppm, was found in S. subalpina that originated from Gedung Songo, Central Java. Those results indicated that more than one Selaginella species originating from Java Island had marked potential for use as medicinal plants.
Co-Authors Adhy Widya Setiawan ADI BASUKRIADI Adi Basukriadi Afri Irawan Afri Irawan, Afri Ahmad Dwi Setyawan Akrom, Akrom Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana ALEX HARTANA Alex Hartana Alex Hartana Alex Hartana Amelia Louisyane Puhili Amelia Luisyane Puhili Andayaningsih, Dwi Andi Madihah Manggabarani Anjar Tri Wibowo Anne Kusumawaty Anne Kusumawaty Arifin Surya Dwipa Irsyam Arifin Surya Dwipa Irsyam Asardi, Istiqoma Aswarina Nasution Awik Puji Dyah Nurhayati Badriah, Siti Nurul Billyardi Ramdhan Budi Prasetyo Budi Prasetyo BUDI SETIADI DARYONO Christyne SPLS Napitu Daniel Happy Putra Deby Arifiani Deby Arifiani, Deby Dede Setiadi Deden Girmansyah Dedy Darnaedi Dedy Darnaedi Dedy Darnaedi DEVI ALVITASARI Devi Alvitasari, Devi Devi Eka Lestari Dorly Dorly Dorly, Dwi Andayaningsih Dwi Putri Ramadhani Dwi Suci Setyaningsih Dwi Sunarti Puspitasari Dwi Sunarti Puspitasari, Dwi Sunarti Effendi, Surianto Eka Setiawan Eko Baroto Walujo Eko Baroto Walujo EKO BAROTO WALUJO Eko Baroto Waluyo Elizabeth A. Widjaja Elizabeth A. Widjaja Ervizal A.M Zuhud Ervizal Amir Muhammad Zuhud Ervizal AMZU Eva Kristinawati Putri Fadel Nugraha Furqon Al Muzakki Furqon Al Muzakki Gunawan Gunawan Gunawan Gunawan Haris Maulani HIMMAH RUSTIAMI Himmah Rustiami Himmah Rustiami Himmah Rustiami Himmah Rustiami, Himmah I Gusti Wayan Murjana Yasa I Putu Gede P. Damayanto I Putu Gede P. Damayanto I PUTU GEDE P. DAMAYANTO I PUTU GEDE P. DAMAYANTO, I PUTU GEDE P. Ibna Hayati IBNUL QAYIM Isna Arofatun Nikmah J PERRY GUSTAFSON Jati Batoro Jati Batoro Junaidi, Muhamad Khoon Meng Wong Kuswata Kartawinata KUSWATA KARTAWINATA Lana Maulana Latifah, Vida Rahma Manggabarani, Andi Madihah Marlina Ardiyani maulana, lana Meitha, Karlia Meliza, Rezika Mentari Putri Pratami Mentari Putri Pratami Mien A. Rifai Mien A. Rifai Miftahudin . Miftahudin Miftahudin Miftahudin Miftahudin Muchlis . Mugi Mumpuni Muhammad Efendi Muhammad Efendi Muhammad Efendi, Muhammad Muhammad Rifqi Hariri Muhammad Rifqi Hariri Muzzazinah Muzzazinah Muzzazinah Muzzazinah Nasrianti Syam NASRIANTI SYAM, NASRIANTI SYAM, NASRIANTI Nasution, Aswarina Nasution, Aswarina Nina Ratna Djuita Novi Novitasari, Risti Nugroho, Giri NUNIK SRI ARIYANTI Nurul Aini Nurul Aini Oktavia Pradikaningrum, Novi Peniwidiyanti Peniwidiyanti Pieter Agusthinus Riupassa Pranata, Syafroni Priyanti Priyanti Priyanti Priyanti Priyanti, Pudji Widodo Puhili, Amelia Luisyane Puspita Dewi Sopyati Putro, Karso Suryo Qothrunnada - Sungkar Rambe, Saidah Putri Ratna Djuita, Nina Rezika Meliza Ridha Mahyuni Ridha Mahyuni Ridha Mahyuni Ridha Mahyuni Risti Novitasari Rizki, Fitri Sri Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Safniyeti Safniyeti Safniyeti Safniyeti, Safniyeti Sartika, Dian Siti Halimatussadiah Sobir Sobir Sobir Sobir Sobir, Sri Endarti Rahayu Sri Endarti Rahayu, Sri Endarti Suharsono Suharsono Suharsono, Sulistijorini Sulistijorini Syadwina Hamama Dalimunthe Syadwina Hamama Dalimunthe Syafroni Pranata Tira Tahnia Titien Ngatinem Praptosuwiryo Titien Ngatinem Praptosuwiryo Titin Ngatinem Praptosuwiryo Tnunay, Ite Morina Yostianti Tri Atmowidi Wakhidah, Anisatu Z. WISNU HANDOYO ARDI WISNU HANDOYO ARDI, WISNU HANDOYO Y. Purwanto Y. Purwanto Yayan Wahyu Candra Kusuma Yohanes Purwanto Yusuf, Adib Fakhruddin Zulhendra zulhendra Zumaidar Zumaidar