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All Journal International Journal of Public Health Science (IJPHS) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Kedokteran Syiah Kuala Majalah Kedokteran Bandung e-Journal Pustaka Kesehatan Jurnal Kesehatan Masyarakat Indonesian Journal of Tropical and Infectious Disease JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Journal of Agromedicine and Medical Sciences JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Elkawnie MIX : Jurnal Ilmiah Manajemen The Indonesian Journal of Occupational Safety and Health Majalah Kedokteran Sriwijaya Universa Medicina Paediatrica Indonesiana Jurnal Bioteknologi & Biosains Indonesia (JBBI) Medical Laboratory Technology Journal Journal Of Vocational Health Studies Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan NurseLine Journal Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Jurnal Kesehatan Prima JPSCR : Journal of Pharmaceutical Science and Clinical Research Indonesian Journal for Health Sciences (IJHS) SRIWIJAYA JOURNAL OF MEDICINE Journal of Health Sciences JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Public Health and Preventive Medicine Archive Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) ASPIRATOR - Journal of Vector-Borne Diseases Studies
Dini Agustina
Departemen Mikrobiologi Fakultas Kedokteran Universitas Jember, Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Efek Penambahan Vitamin C terhadap Aktivitas Klindamisin dalam Menghambat Pertumbuhan Streptococcus pneumoniae secara In Vitro Bagus Dwi Kurniawan; Dini Agustina; Erfan Efendi
Pustaka Kesehatan Vol 6 No 2 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i2.9744

Abstract

Pneumonia is one of the lower respiratory tract infection involving respiratory bronchioles and alveoli that mostly caused by Streptococcus pneumoniae.Clindamycin is one of the drug of choice for pneumoniae. This drugs inhibits the growth of S. pneumoniae by producing ROS (Reactive Oxygen Species). Recent study has shown that vitamin C could generate ROS leading to DNA-damage on bacteria. The aims of this study was to know the possible benefit effect of adding vitamin C towards clindamycin inhibition activity against the growth of S. pneumoniae in vitro. This research was conducted by using 7 groups which consist of negative control (aquades), positive control (clindamycin), and test group 1 until 5 that given the combination of clindamycin and vitamin C as follows 2,5 mg/ml; 5 mg/ml; 10 mg/ml; 20 mg/ml; and 40 mg/ml. The inhibition effect of growing S. pneumoniae shown by the inhibition zone diameter. The average of inhibition zone on negative control, positive control, and test group were 5; 24,12; 24,62; 26,08; 27,58; 28,74; and 29,76 mm. These result show a significant correlation based on Pearson test (the coefficient correlation’s result is +0,910). The logarithmic regression revealed the minimal concentration of vitamin C was 1,767 mg/ml. It can be concluded that vitamin C increased the activity of clindamycin by inhibiting the growth of S.pneumoniae in vitro. Keywords: Streptococcus pneumoniae, ROS, Clindamycin, Vitamin C
Respon Imunogenitas Protein Permukaan 19 kDa Streptococcus pneumoniae (Immunogenicity Response of Streptococcus pneumoniae 19 kDa Surface Protein) Muhammad Dimas Arya Candra Permana; Dini Agustina; Bagus Hermansyah
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Pneumonia is the main common cause of children death in the world. Microbiological studies found the major causes of pneumonia is Streptococcus pneumoniae. Infection of S.pneumoniae influenced by several virulence factors, one of which is surface protein. The purpose of this study was to determine the immunogenicity of 19 kDa S.pneumoniae surface protein through hemagglutination inhibition and adhesion inhibition test. Adhesion inhibition test used antibodies that is formed from surface protein, to inhibit bacterial adhesion to the enterocytes. Hemagglutination inhibition test also used antibodies that formed from surface protein, to inhibit the agglutination of erythrocytes caused by S.pneumoniae. The results showed that antibodies, which was formed from 19 kDa S.pneumoniae surface protein, were able to inhibit hemagglutination process up to 1X dilution. Adhesion inhibition test showed that the higher antibody dilution, the greater the adhesion index. Pearson correlation analysis of the adhesion test showed that there was a significant correlation between antibody dilution with bacterial adhesion index (R= -0,787; p=0,036). It can be concluded that the 19 kDa surface protein of S.pneumoniae was immunogenic.   Keywords: Streptococcus pneumoniae, surface protein, antibody
Efektivitas Kombinasi Vankomisin dan Vitamin C terhadap Pertumbuhan MRSA (Methicillin Resistant Staphylococcus aureus) (Effectiveness of Vancomycin and Vitamin C Combination on MRSA (Methicillin Resistant Staphylococcus aureus) Growth) Ulin Nuha Jazmin; Dini Agustina; Rony Prasetyo
Pustaka Kesehatan Vol 6 No 1 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i1.6796

Abstract

Nephrotoxicity due to prolonged use of vancomycin becomes a big issue in patients with MRSA infections and the use of vitamin C has been proved to decrease this side effect. Vitamin C with its combination with antibiotic will halt MRSA growth by forming Reactive Oxygen Species (ROS). This study aimed to determine the effect of vancomycin and vitamin C combination in order to halt the MRSA growth and the minimum concentration of vitamin C which can decrease the Minimum Inhibitory Concentration (MIC) of vancomycin. This study used a quasi experimental design with 7 groups, 1 as negative control which only given vancomycin and 6 other groups which given vancomycin and vitamin C with following concentration 0,4; 0,8; 1,6; 3,2; 6,4; and 12,8 mg/mL. We used microdilution method with 4 repetitions to measure the MIC value. This study revealed that there were no difference between control and other groups, the MIC of vancomycin is 1 µg/mL. Combination effect was measured by Fractional Inhibitory Concentration Index (FICI) calculation which showed indifferent effect to all groups. This study concluded that combination of vancomycin and vitamin C effect has no difference with control group and by adding vitamin C until 12,8 mg/mL did not decrease MRSA growth. Keywords: MRSA, vancomycin, vitamin C, Minimum Inhibitory Concentration (MIC)
Efek Ekstrak Air Kulit Buah Delima (Punica granatum L) terhadap Salmonella typhimurium secara In Vivo (The Effect of Pomegranate (Punica granatum L) Peel Aqueous Extract on Salmonella typhimurium In Vivo) Anastasia Citra Purwani; Dini Agustina; Yuli Hermansyah
Pustaka Kesehatan Vol 3 No 2 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Salmonella typhi infection is commonly treated by an antimicrobial agent such as levofloxacin. At the moment, herbal usage has risen as an alternative therapy. Pomegranate (Punica granatum L) is a commonly used herb which contains flavonoid as its major active compound. Aqueous extract of pomegranate peel had been known for its antimicrobial effect to S. typhi on in vitro study. The aim of this research was to investigate the activity of aqueous extract of pomegranate peel to S. typhimurium in vivo and find the antimicrobial effect of different concentrations. The study was conducted by using typhoid fever model in mice infected by S. typhimurium. Each mice was treated with aqueous pomegranate peel extract in three consentrations: 0,65 mg/ml, 1,3 mg/ml and 2,6 mg/ml for treatment groups and levofloxacin 1,3 mg/ml for positive control group. Ileum of each mice was isolated and cultured. The result of post hoc LSD test showed that negative control group significantly different with treatment group and the result of Pearson test showed p=0,000 and r = -0,865. In conclusion, aqueous extract of pomegranate peel had an antimicrobial activity on S. typhimurium in vivo where the higher extract concentration the lesser number of S. typhimurium colony.   Keywords: Antimicrobial, Pomegranate, Salmonella typhimurium
Daya Antibakteri Kombinasi Kitosan Cangkang Udang Putih (Litopenaeus vannamei) dan Siprofloksasin terhadap Salmonella typhi (Antibacterial Activity of Combination of White Shrimp (Litopenaeus vannamei) Shells Chitosan and Ciprofloxacin against Salmonella Sarah Marsa Tamimi; Dini Agustina; Cicih Komariah
Pustaka Kesehatan Vol 5 No 2 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

yphoid fever is an acute infectious disease in the intestinal tract caused by Salmonella typhi. The prevalence of typhoid fever in Indonesia in 2013 was 81% per 100,000 population. Ciprofloxacin is a large-spectrum antibiotics, but in some countries ciprofloxacin has been known to be resistant to S. typhi. Therefore, other methods are needed to improve the effectiveness of ciprofloxacin by combining with chitosan. Chitosan is a polysaccharide which known to be effective in some positive and negative Gram bacteria and able to overcome the resistance of some antibiotics. The research objective was to determine the effect of antibacterial and Minimum Inhibitory Concentration (MIC) of the combination of Litopenaeus vannamei chitosan and ciprofloxacin against S. typhi. The method used to obtain the value of the MIC was microdilution method with quasy-experimental research design and posttest only control group design. MIC which already been obtained then used to find the Fractional Inhibitory Concentration Index (FICI) using a checkerboard test to interpret the effect of the combination. The MIC combination showed different results in each repetition that chitosan: 15.63 to 62.5 µg/mL and ciprofloxacin from 0.03 to 0.5 µg/mL and known to have a smaller value than the MIC single. While the value of FICI from 5 repetitions was 0.476. So, it could be concluded that the combination of chitosan and ciprofloxacin has an antibacterial effect on S. typhi and have a synergistic combination effect. Keywords: Salmonella typhi, chitosan, ciprofloxacin, microdilution method, checkerboard test.
Immunodetection of Adhesin Pili Protein 38.6 kDa K. pneumoniae Using Western Blot Dini Agustina; Diana Chusna Mufida; Nurul Indah Saffanah
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 2 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i2.10127

Abstract

Abstract: Klebsiella pneumoniae is a pathogen that causes infections in communities and hospitals. These bacteria have many virulence factors that play an important role in the pathogenicity of infection and antibiotic resistance. Pili protein Klebsiella pneumoniae with 38.6 kDa as one of the virulence factors, that have roles as hemagglutinin dan adhesin protein so potentially as a vaccine candidate. This research aimed to immunodetection pili protein 38.6 kDa using Western Blot.  Western Blot was used to detect that protein with specific primary antibodies. These antibodies are obtained from mice serum-induced intraperitoneally with Pili protein 38.6 kDa Klebsiella pneumoniae. Protein bands that appear on the membrane of Western Blot results are proteins with a molecular weight of 85.6 kDa, 65.5 kDa, 46.9 kDa, and 29.4 kDa. This study concludes that Pili protein 38.6 kDa Klebsiella pneumoniae as the target protein does not appear in the Western Blot result.Abstrak: Klebsiella pneumoniae adalah patogen yang menyebabkan infeksi di komunitas maupun rumah sakit. Bakteri tersebut memiliki banyak faktor virulensi yang berperan penting dalam patogenisitas terjadinya infeksi dan resistensi antibiotik. Protein pili Klebsiella pneumoniae dengan 38,6 kDa sebagai salah satu faktor virulensi, memiliki peran sebagai protein hemagglutinin dan adhesin sehingga berpotensi sebagai kandidat vaksin. Tujuan penelitian ini untuk imunodeteksi protein pili 38,6 kDa dengan menggunakan Western Blot. Western Blot digunakan untuk mendeteksi protein itu dengan spesifik antibodi primer. Antibodi ini diperoleh dari serum tikus yang telah diinduksi secara inteperitoneal dengan protein Pili 38,6 kDa Klebsiella pneumoniae. Band protein yang muncul pada membran hasil Western Blot adalah protein dengan berat molekul 85,6 kDa; 65,5 kDa; 46,9 kDa dan 29,4 kDa. Dari penelitian ini dapat disimpulkan bahwa protein Pili 38.6 kDa Klebsiella pneumoniae sebagai protein target tidak muncul.
PENGARUH PERSONALISASI IKLAN ONLINE TERHADAP SIKAP DAN MINAT BELI KONSUMEN Dini Agustina; Mukhamad Najib; Budi Suharjo
MIX: JURNAL ILMIAH MANAJEMEN Vol 6, No 3 (2016): MIX: Jurnal Ilmiah Manajemen
Publisher : Universitas Mercu Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.605 KB)

Abstract

Abstract. Personalized online advertisement is expected to minimize irritation of theadvertisements, and it may be beneficial to help consumers in searching informationbefore they are making a purchase decision. At present, consumers are having a diverseattitude toward the personalized online advertising, some are in favor, some are neutraland some are actually opposed to. Consumer's attitude play an important role consumerpurchase intention. Therefore, it is very critical for the personalized online advertisingto provide more value added to the consumer based on their purchase behavior bygiving the relevant information, while at the same time keeping the consumer's privacy.Despite of a lot of effort has been put in place to improve the effectiveness ofpersonalized online advertising, it has yet gained more positive response from theconsumer. This research is using the survey method that involving 200 respondents anddata analysis is using SEM (Structural Equation Modeling).
THE RELATION OF PERSONAL HYGIENE WITH THE RISK OF OCCURRENCE OF SOIL-TRANSMITTED HELMINTH CO-INFECTION IN TB PATIENTS IN THE WORKING AREA OF THE JENGGAWAH HEALTH CENTER IN JEMBER REGENCY Ellen Ocktavironita; Bagus Hermansyah; Angga Mardro Raharjo; Diana Chusna Mufida; Dini Agustina; Muhammad Ali Shodikin
Majalah Kedokteran Sriwijaya Vol 52, No 2 (2020): Majalah Kedokteran Sriwijaya
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/mks.v52i2.11977

Abstract

Tuberculosis (TB) and soil-transmitted helminth (STH) infections are infectious diseases with a high prevalence in Indonesia. STH co-infection in TB patients can worsen the prognosis of TB disease due to the dysregulation of the immune response. The high prevalence of STH infections in Indonesia is caused by environmental factors such as poor personal hygiene habits. Jenggawah Subdistrict is an area with a high number of TB cases in Jember Regency and most of the area is in the form of agricultural fields and plantations that have a suitable humidity and temperature for the development of STH. The purpose of this study was to determine the relationship of personal hygiene risk factors with the risk of STH co-infection in TB patients in the work area of the Jenggawah Health Center in Jember Regency. This study used an observational analytic design with a cross sectional method and was conducted from September to December 2019. The research was conducted at the TB Polyclinic in Jenggawah Health Center in Jember Regency with a total sample of 26 respondents who were given a personal hygiene questionnaire. Stool examination was conducted at the Laboratory of Parasitology FK UNEJ using sedimentation and flotation methods. The results showed the incidence of STH co-infections was 15.3%, good personal hygiene was 57.7%, and bad personal hygiene was 42.3%. Fisher exact test results showed that there was no significant relationship between personal hygiene risk factors and the risk of the occurrence of STH co-infection in TB patients in the working area of the Jenggawah Health Center in Jember Regency (p = 1,000).
The Role of Pili Protein 38,6 kDa Klebsiella pneumoniae as a Hemagglutinin and Adhesin Protein which Serves as a Virulence Factor Regina Finka; Dini Agustina; Dwita Aryadina Rachmawati; Enny Suswati; Diana Chusna Mufida; Ali Shodikin
Journal of Agromedicine and Medical Sciences Vol 5 No 2 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i2.9558

Abstract

Klebsiella pneumoniae is an infectious bacteria in various parts of the body. The presence of proteins in pili that will bind to cell surface receptors (adhesin proteins) and cell membrane sugar molecules (hemagglutinin proteins) is a very influential factor in the ability of K. pneumoniae to enter the human body. The purpose of this study was to determine the role of pili 38.6 kDa K. pneumoniae protein as a hemagglutinin and adhesin protein which functions as a virulence factor. This type of research is pure experimental research using the electrophoresis method (SDS-PAGE) to obtain protein. The protein obtained was then tested for hemagglutination and adhesion test using erythrocyte cells and enterocytes of BALB / C mice to determine their role as hemagglutinin and adhesin proteins. Conclusion of this study pili protein 38.6 kDa Klebsiella pneumoniae acts as a hemagglutinin and adhesion protein which functions as a virulence factor. Keywords: Klebsiella pneumonia, 38,6 kDa Protein, Hemagglutinin, Adhesin
Modulation of Antibiotic Activity Against Streptococcus pneumoniae by N-acetylcysteine and ascorbic acid Dini Agustina; Bagus Dwi Kurniawan; Intan Palupi
Journal of Agromedicine and Medical Sciences Vol 2 No 3 (2016)
Publisher : Faculty of Medicine, University of Jember

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Abstract

Streptococcus pneumoniae is one of the bacterial pathogens that cause pneumonia. Pneumonia is the biggest cause of infant mortality in Indonesia around 23%. The main therapy pneumonia is antibiotics, but in some cases combined with ascorbic acid or N-acetylcysteine, which serves as a complementary therapy. The purpose of this study was to determine the effect of adding ascorbic acid or N-acetylcysteine ​​on antibiotics towards the growth of S. pneumoniae in vitro. The method used in this research is the method of germ-disc diffusion susceptibility test with S. pneumoniae growth inhibition is indicated by the formation of inhibition zone. Positive controls used clindamycin and erythromycin. The average diameter of inhibition zone on the negative control, positive control, and treatment of 1 to 5 on the addition of clindamycin with ascorbic acid are respectively 5; 24.12; 24.62; 26.08; 27.58; 28.74; and 29.76 mm. The data has a strong correlation to the Pearson test (R=+0,910). On a logarithmic regression analysis found minimal concentration of ascorbic acid can inhibit is 1.767 mg/ml. While the addition of erythromycin with N-acetylcysteine ​​are respectively 28.02; 25.62; 23.46; 22.42; and 21.68 mm. Pearson correlation test results of these data are the opposite relationship between the diameter of inhibition zone and the concentration of N-acetylcysteine ​​with a very strong correlation coefficient. Logarithmic regression of the minimum concentration of N-acetylcysteine ​​which may decrease the antibacterial activity of erythromycin inhibit the growth of Sreptococcus pneumoniae amounted 1,66mg/ml. It is concluded that ascorbic acid increases the activity of clindamycin in inhibiting the growth of S. pneumoniae in vitro and N-acetylcysteine ​​may decrease the antibacterial activity of erythromycin in inhibiting the growth of S. pneumoniae in vitro. Keywords: S. Pneumoniae, ascorbic acid, N-asetilsitein, inhibition zone
Co-Authors Alfianti, Nita Ali Santosa Anaditya Wahyu Kumudhaningsih Anastasia Citra Purwani Angga Mardro Raharjo Angga Mardro Raharjo, Angga Mardro Astuti Setyawardani Bagus Dwi Kurniawan Bagus Dwi Kurniawan Bagus Hermansyah Bagus Hermansyah Budi Suharjo Cahyaningrum, Endiningtyas Cholis Abrori, Cholis Cicih Komariah De Rorna, Masrahma Reinataya Denaneer Rahmadatu Desie Dwi Wisudanti Diana C. Mufida Diana Chusna Mufida Diana Chusna Mufida Dion Krismashogi Dharmawan Dwita Aryadina Rachmawati El Milla, Kardiana Izza Ellen Ocktavironita Elvia Rahmi Marga Putri Enny Suswanti Enny Suswati ENNY SUSWATI Erfan Efendi Erma Sulistyaningsih Fitri, Nadya Eka Gama Wisnu Sanjaya Habib Mustofa Habibbur Rochman Salim Habibi, Ali Hamzah, Salsabilla Maula Zalfa El Hanifa Rizki A.S. Hibatulloh, Muhammad Farhan Himma, Alyatul I Nyoman Semita Iftinan, Karenzha Ika Rahmawati Sutejo Intan Palupi Irawan Fajar Kusuma Jauhar Firdaus Kirana Nadyatara Kristianningrum Dian Sofiana Kusiyanto Taslim, Fitranda Laila Rizqi Kurniawati Lailatis Shofia Laksmi Indreswari M. Ali Shodikin Mei Liana Wati Miftakhul Huda Muhammad A. Shodikin Muhammad Ali Shodikin Muhammad Dimas Arya Candra Permana Mukhamad Najib Nastiti Bekti Utami Nihayah Lukman Nisrina Salsabila Firmansyah Nita Alfianti Noorhamdani AS Noorhamdani AS Nugraha, Bima Setia Sandya Nurul Indah Saffanah Nurul Indah Saffanah Nurus Saffana Yulianto Pralampita, Pulong Wijang Primadana, Aditya Putra Uta, Diego Rafi Putra, Muhamad Dwi Eka Putra, Yudha Ananta Khaerul Ramadhani, Alfiyah Regina Finka Rinda Yanuarisa Rony Prasetyo Ryan Ravi Is Syahputra S.Pd. M Kes I Ketut Sudiana . Sa'adah, Ajeng Samrotu Sakinah, Elly N Salsabila, Yuna Annisa Salsabilla Maula Zalfa El Hamzah Saputra, Antonius Dwi Sarah Marsa Tamimi Semita, I Nyoman septa surya wahyudi, septa surya Setyawardani, Astuti Shodikin, M. Ali Shofia, Lailatis Siti Marissa Aisyah Siti Zulaikha Sumarno Retoprawiro Supangat Supangat Syahputri, Siti Ananda Hardita Tristianti, Farmitalia Nisa Ulfa Elfiah Ulin Nuha Jazmin Widhiasari Normaningtyas Wiwien Sugih Utami Yudha Nurdian Yuli Hermansyah Yuli Rahmantia, Vania Yuna Annisa Salsabila Yunita Armiyanti