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All Journal International Journal of Public Health Science (IJPHS) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Kedokteran Syiah Kuala Majalah Kedokteran Bandung e-Journal Pustaka Kesehatan Jurnal Kesehatan Masyarakat Indonesian Journal of Tropical and Infectious Disease JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Journal of Agromedicine and Medical Sciences JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Elkawnie MIX : Jurnal Ilmiah Manajemen The Indonesian Journal of Occupational Safety and Health Majalah Kedokteran Sriwijaya Universa Medicina Paediatrica Indonesiana Jurnal Bioteknologi & Biosains Indonesia (JBBI) Medical Laboratory Technology Journal Journal Of Vocational Health Studies Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Jurnal Kesehatan Prima Indonesian Journal for Health Sciences (IJHS) SRIWIJAYA JOURNAL OF MEDICINE Journal of Health Sciences Action Research Literate (ARL) Jurnal Lingkar Mutu Pendidikan JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Public Health and Preventive Medicine Archive Berita Kedokteran Masyarakat Jurnal Indonesia Sosial Sains Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) ASPIRATOR - Journal of Vector-Borne Diseases Studies Jurnal Lingkar Mutu Pendidikan

Dini Agustina

Departemen Mikrobiologi Fakultas Kedokteran Universitas Jember, Jember

Author-ID : 254299

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Bacterial and parasitic contamination of raw vegetable in Jember regency, Indonesia : potential risk for food-borne diseases Diana Chusna Mufida; Elvia Rahmi Marga Putri; Dini Agustina; Enny Suswati; Muhammad Ali Shodikin; Yunita Armiyanti; Bagus Hermansyah; Wiwien Sugih Utami; Angga Mardro Raharjo
International Journal of Public Health Science (IJPHS) Vol 11, No 4: December 2022
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v11i4.21875

A raw vegetable is one of the transmission factors in human food-borne infection. Contamination might start from the plantation, distribution, until at serving time in the dining table. A higher level of contamination will increase the risk of food-borne illness. The study aims to determine bacteria and parasite contamination in some raw vegetables that people usually consume in Jember Regency, East Java, Indonesia. In this study, we collected raw vegetable from eight traditional markets. We chose randomly at each market five samples of vegetables that usually directly consumed, like lettuce, tomato, cabbage, basil, long bean, and cucumber. Pathogens we identified limited on bacteria and parasite like helminth and protozoan group. This study showed that 91% contaminated by E. coli, 84% by Staphylococcus aureus, and 79% contaminated by Vibrio cholerae. Besides bacteria, 36% of samples contaminated by the helminth group, and protozoa contaminated 27%. The most vegetables contaminated was lettuce, even in bacterial or parasitic contamination. This study concluded that there is contamination in raw vegetables usually directly consumed in Jember Regency. It will be a potential risk factor for food-borne illness in the future.

PROTEIN PILI 96,4 kDa Klebsiella pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Laila Rizqi Kurniawati; M Ali Shodikin; Dini Agustina; Kristianningrum Dian Sofiana
Indonesian Journal for Health Sciences Vol 5, No 1 (2021): March
Publisher : Universitas Muhammadiyah Ponorogo

Klebsiella pneumoniae merupakan bakteri genus Klebsiella dalam famili Enterobactericeae. Klebsiella pneumoniae menyebabkan infeksi serius. Faktor virulensi penting dalam perlekatan awal bakteri untuk menginfeksi inang pili dan Outer Membrane Protein (OMP). Pili sebagai faktor virulensi dapat melekat kuat secara spesifik dan mampu menghindari pembersihan yang dilakukan oleh sel inang. Kemampuan adhesi suatu bakteri dipengaruhi oleh kemampuannya dalam mengagglutinasi eritrosit. Berdasarkan studi pendahuluan yang telah dilakukan, berat molekul protein pili yang muncul dari hasil elektroforesis SDS-PAGE yaitu 96,4 kDa memiliki pita tebal dan berkonsentrasi tinggi serta belum ada penelitian membuktikan bahwa protein pili 96,4 kDa merupakan protein hemaglutinin dan adhesin.Tujuan penelitian ini untuk mengetahui peran protein pili 96,4 kDa bakteri Klebsiella pneumoniae sebagai protein hemaglutinin dan adhesin. Penelitian ini dilakukan dengan mengisolasi protein pili Klebsiella pneumoniae kemudian dilakukan uji aglutinasi menggunakan sel eritrosit mencit dan uji adhesi menggunakan sel enterosit mencit. Hasil yang diperoleh dianalisis menggunakan Saphiro-Wilk. Hasil uji hemaglutinasi dengan menggunakan sel eritrosit mencit didapatkan titer hemaglutinasi paling tinggi untuk berat molekul protein pili 96,4 kDa adalah pada titer 1. Indeks adhesi dengan titer pengenceran memiliki hubungan yang bermakna yaitu 0,016 (p 0,05) dengan nilai r = -0,847, sehingga disimpulkan protein pili 96,4 kDa Klebsiella pneumoniae merupakan protein hemaglutinin dan adhesin.

Characterization of pili protein with molecular mass of 85 kDa Escherichia coli as an adhesin and a hemagglutinin Dini Agustina; Siti Marissa Aisyah; Ika Rahmawati Sutejo; Diana Chusna Mufida
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art5

Background: Escherichia coli (E. coli) is a rod-shaped gram-negative bacterium included in Multi Drug Resistant (MDR) bacteria. There are several strategies in place to prevent and treat E. coli bacterial infections to reduce some the incidences of MDR. One of them is the development of a protein-based vaccine. Pili is one of virulence factors in E. coli surface proteins that can mediate attachments of bacteria to host cells (adhesin or hemagglutinin) and can be used as protein-based vaccine candidates. Objective: This study aims to determine characterization of pili protein with molecular mass of 85 kDa Escherichia coli bacteria as an adhesin and a hemagglutinin. Methods: This study is a true experimental laboratories and a descriptive study to determine roles of pili protein with molecular mass of 85 kDa as an adhesin and a hemagglutinin. Samples used in this study were a stock of E. coli in the Microbiology Laboratory Faculty of Medicine, Universitas Negeri Jember (UNEJ). A hemagglutination test in this study used mouse erythrocytes while an adhesion test used mouse enterocytes. Isolation and purification of E. coli pili protein, isolation of mouse erythrocytes, isolation of mouse enterocytes, the hemagglutination test and the adhesion test were methods in this study. A data analysis with a correlation-regression was used to determine relationships between the adhesion index and titer pili 85 kDa with a limit of significance, 0.05 (p <0.05). Results: The hemagglutination test showed no ability of agglutination with the formation of red aggregate points starting from the first dilution. An analysis with Pearson correlation indicated a relationship between titer pili 85 kDa E. coli and adhesion index (p = 0.009; R = -0.921).Conclusion: Pili protein 85 kDa E. coli plays a role as an adhesin protein and do not have a role as a hemagglutinin protein.

Correlation between physical characteristics of the indoor environment toward the concentration of fungi in the inpatient rooms of dr. Soebandi Regional Hospital Jember, East Java Salsabilla Maula Zalfa El Hamzah; Astuti Setyawardani; I Nyoman Semita; Dini Agustina
Public Health and Preventive Medicine Archive Vol. 8 No. 1 (2020)
Publisher : Universitas Udayana

Background and purpose: Air quality is an important determinant of a healthy life. One indicator of air pollutants in the room is fungi, which in hospital setting may cause nosocomial infection. This research aims to find out a correlation between physical characteristics of the indoor environment toward the concentration of fungi in the inpatient rooms of dr. Soebandi Regional Hospital Jember, East Java. Methods: This research uses a cross-sectional method with a total of four inpatient rooms. We measured physical room characteristicsincluding room temperature, humidity, light intensity, and room density. The concentration of fungi was measured by collecting air sample using passive air sampling method with a potato dextrose agar as the media to optimize the fungal growth. The data was processed with Saphiro-Wilk and Pearson correlation analysis using SPSS 25.0. Results: The ranges of room temperature and humidity were 29.6- 32.2°C and 41-59%, respectively; while the light intensity and roomdensity were 10-30 and 1.88-2.38 person/10 m2. The bivariate analysis showed a correlation between two components of physical characteristic of the indoor environment, room temperature and room density, with the concentration of fungi. Conclusion: There is a correlation between room temperature and room density with the concentration of fungi in the inpatient rooms of dr. Soebandi Regional Hospital Jember. The management of hospitals should take measures to improve the air quality within inpatient rooms.

Hubungan antara Higiene Perorangan dan Kejadian Koinfeksi Cacingan pada Penderita Tuberkulosis Lailatis Shofia; Bagus Hermansyah; Enny Suswati; Dini Agustina; Diana Chusna Mufida; Muhammad Ali Shodikin
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Vol 4, No 1, 2021
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v4i1.98

Tuberkulosis (TB) dan cacingan merupakan penyakit infeksi dengan jumlah penderita yang banyak di Indonesia. Tingginya prevalensi cacingan di Indonesia memungkinkan terjadinya koinfeksi STH pada pasien TB paru yang menyebabkan imunitas anti M. tuberculosis menurun sehingga respon terhadap pengobatan tuberkulosis menjadi tidak maksimal. Cacingan salah satunya dipengaruhi oleh higiene perorangan. Tujuan penelitian ini adalah untuk menganalisis hubungan antara higiene perorangan dan kejadian koinfeksi cacingan pada penderita TB di Kecamatan Tempurejo Kabupaten Jember. Jenis penelitian ini adalah penelitian observasional dengan desain analitik cross sectional. Populasi yang digunakan adalah pasien TB di Kecamatan Tempurejo dalam periode waktu September – Oktober 2019. Data yang digunakan dalam penelitian ini adalah data primer berupa hasil wawancara dan hasil pemeriksaan feses. Pemeriksaan feses dilakukan menggunakan metode sedimentasi dan floatasi. Analisis data dilakukan menggunakan uji Fisher. Hasil penelitian ini menunjukkan bahwa kejadian koinfeksi cacingan pada pasien TB di Kecamatan Tempurejo Kabupaten Jember sebesar 9,67% dan disebabkan oleh dua spesies STH yaitu A. lumbricoides (66,7%) dan Hookworm (33,3%). Higiene perorangan responden terdiri atas higiene baik (64,5%) dan higiene buruk (35,5%), dimana 66,7% kejadian koinfeksi cacingan terjadi pada responden dengan higiene perorangan buruk. Hasil uji Fisher menunjukkan bahwa tidak ada hubungan yang signifikan antara higiene perorangan dengan kejadian koinfeksi cacingan pada pasien TB di Kecamatan Tempurejo Kabupaten Jember (p-value sebesar 0,281).

Inhibition of Klebsiella pneumoniae adhesion in mice enterocytes by antibodies of hemagglutinin pili protein with MW 12.8 kDa of Klebsiella pneumoniae Dini Agustina; Sumarno Retoprawiro; Noorhamdani AS
Journal of Tropical Life Science Vol. 4 No. 1 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Klebsiella pneumoniae as one of the most common cause of VAP is also the second most common cause of community- and hospital-acquired gram negative bloodstream infection. The process of infection caused by direct contact with infectious agents begins with the host cell adhesion process either by pili or by afimbria adhesin (AFA). There were no reported researches on the hemagglutinin pili protein of K.pneumoniae as adhesion factors.This study was conducted in order to determine the hemagglutinin pili protein of K.pneumoniae, polyclonal antibody produced from pili protein immunization, and its ability to inhibit K.pneumoniae adhesion in mice enterocytes. Adhesion inhibition test used HA antibody with the implementation of dose dilutions of 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200 and 0 (control). While immunocytochemistry test used HA pili protein with the implementation of dose dilutions of 1/10000, 1/20000, 1/40000, 1/80000, 1/160000, 1/320000 and 0 (control). Hemagglutinin pili protein found in K.pneumoniae had MW 12.8 kDa. Pearson correlation analysis of adhesion test showed there were significant correlation between antibody dilution titer with bacterial adhesion (p = 0.032, R = -0.797). Furthermore, Anova analysis of IT showed that there were significant differences between the various dilution titer with antigen-antibody reaction (p=0.000). Antibody of hemagglutinin pili protein with MW 12.8 kDa of K.pneumoniae can inhibit the adhesion of K.pneumoniae to the enterocytes of mice.

Spontaneous pneumothorax in a child with miliary tuberculosis: a case report Muhammad Ali Shodikin; Dini Agustina
Paediatrica Indonesiana Vol 62 No 5 (2022): September 2022
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/pi62.5.2022.364-6

Spontaneous pneumothorax is a rare but fatal complication of miliary tuberculosis. We report a 13-year-old boy presenting with shortness of breath. He was diagnosed with miliary tuberculosis with spontaneous pneumothorax, which showed significant improvement after the insertion of a thoracostomy tube and anti-tuberculosis drug therapy.

Pili Protein 65.5 kDa of Klebsiella pneumoniae Induced a Decrease in IL-10 in Mice Dini Agustina; Mei Liana Wati; Desie Dwi Wisudanti; Muhammad Ali Shodikin; Diana Chusna Mufida; Enny Suswati
Majalah Kedokteran Bandung Vol 54, No 3 (2022)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v54n3.2690

Klebsiella pneumoniae is one of the leading cause of nosocomial infection worldwide with clinical isolates mostly found in Multi-Drug Resistant (MDR) or Extensively Drug-Resistant (XDR). This makes therapeutic options due to this bacteria limited. Several studies have shown that the pili protein of K. pneumoniae with different molecular weights plays a role in the virulence factor, as they are hemagglutinin and adhesin proteins. Pili protein 65.5 kDa of K. pneumoniae can be considered as the antigen candidate for vaccines. This study aimed to determine the immune response based on the IL-10 level as induced by pili protein 65.5 kDa K. pneumoniae. This study was conducted from October 2020 to March 2021 at the Faculty of Mathematics and Natural Sciences and Faculty of Medicine of Jember University. This was true experimental with post-test only controlled group design on 27 male BALB/c mice which divided into 3 groups: K1, K2, and K3. The groups were induced with sterile PBS, pili protein and Freund’s adjuvant, and Freund’s adjuvant only, respectively. Samples were drawn through cardiac puncture, then serum was taken to measure the IL-10 levels using the ELISA method. Hemagglutination test showed a positive result with 1/8 as the highest titer. Results showed that the mean IL-10 levels were 290.92±45.33; 235.05±44.53; 218.54±64,81 for K1, K2, and K3, respectively. One-way ANOVA test results showed a statistical difference between groups (p=0,019). This research shows that pili protein 65.5 kDa K. pneumoniae decreases the IL-10 level. However, when it is provided with the Freund’s Adjuvant, there is no proof of the immunogenicity when compared to the control group.

Analysis of Milking Hygiene and Its Association to Staphylococcus Aureus Contamination in Fresh Cow Milk Nurus Saffana Yulianto; Yunita Armiyanti; Dini Agustina; Bagus Hermansyah; Wiwien Sugih Utami
JURNAL KESEHATAN LINGKUNGAN Vol. 15 No. 4 (2023): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v15i4.2023.275-282

Introduction: Staphylococcus aureus foodborne disease is caused by the consumption of food contaminated with Staphylococcus aureus enterotoxins. Milk is a type of food that contains many nutrients but is susceptible to contamination by Staphylococcus aureus. Jember is one of the districts in East Java that produces cow milk. Previous research showed that the level of milk consumption in the community in 2018 was 3.1 kg/capita/year and 42% consumed pasteurized milk, which has lower quality and may still be contaminated with bacteria. The purpose of this study was to determine the relationship between milking hygiene and Staphylococcus aureus contamination. Methods: A cross-sectional study was conducted involving 36 cow milkers with traditional milking, selected by total sampling. The independent variable was milking hygiene and the dependent variable was Staphylococcus aureus contamination. Milking hygiene practice data were collected through direct observation using a questionnaire. Staphylococcus aureus contamination data were tested using Total Plate Count (TPC) and identified using Gram staining and catalase tests. Results and Discussion: The TPC test results showed that 61.1% of cow's milk fulfilled the Indonesian National Standard for Staphylococcus aureus contamination. There was a relationship between milking hygiene and Staphylococcus aureus contamination. The most influential milking hygiene was cage, udder, and teat hygiene. Conclusion: Staphylococcus aureus contamination did not meet the standards. Therefore, improving the sanitation and hygiene of cages, as well as udder and teat hygiene by dairy farmers, is necessary.

ANTIBIOGRAM OF PATIENTS WITH DIABETIC FOOT AT DR. SOEBANDI REGIONAL HOSPITAL OF JEMBER, INDONESIA Suswati, Enny; Mustofa, Habib; Hermansyah, Bagus; Agustina, Dini; Sakinah, Elly N
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 17 No 1 (2023): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v17i1.5753

Diabetic foot is a complication of diabetes mellitus that uses antibiotics to control the infection as the primary therapy. This study aimed to determine the type of bacteria and antimicrobial sensitivity patterns in diabetic foot. The samples were taken from the medical records of the patients with diabetic foot, who performed the swab culture and antimicrobial susceptibility testing in the inpatient and outpatient care in dr. Soebandi Regional Hospital. The samples used were the patients diagnosed with diabetic foot from January 1, 2014, to December 31, 2018. The data in this study were univariately analyzed. Forty-three pathogens were isolated from 40 patients with 12 ESBL isolates. The most common bacteria found were Gram-negative (90.7%), including Escherichia coli (33.33%), Klebsiella pneumoniae (12.82%) Pseudomonas aeruginosa (12.82%), Enterobacter cloacae (7.69%) and Proteus mirabilis (7.69%). Then, there were also 9.3% Gram-positive bacteria of all isolates, with Staphylococcus aureus as the most dominant species (50%). The antibiotic antimicrobial susceptibility testing also showed that Imipenem, amikacin, fosfomycin, cefoxitin, and netilmicin were the most sensitive antibiotics. The most common type of bacteria found was Escherichia coli, while the antibiotic still sensitive in most bacteria was imipenem.

Co-Authors Ali Santosa Anaditya Wahyu Kumudhaningsih Anastasia Citra Purwani Angga Mardro Raharjo Angga Mardro Raharjo, Angga Mardro Aprilya Pratiwi, Nadilla Arief Subhan Aryasatya, Dimas Widyadhana Bhanu Astuti Setyawardani Bagus Dwi Kurniawan Bagus Dwi Kurniawan Bagus Hermansyah Bagus Hermansyah Budi Suharjo Cahyaningrum, Endiningtyas Cholis Abrori, Cholis Cicih Komariah De Rorna, Masrahma Reinataya Denaneer Rahmadatu Desie Dwi Wisudanti Diana C. Mufida Diana Chusna Mufida Diana Chusna Mufida Dina Helianti Dion Krismashogi Dharmawan Dua , Mikhael Dwita Aryadina Rachmawati El Milla, Kardiana Izza Ellen Ocktavironita Elvia Rahmi Marga Putri Enny Suswanti ENNY SUSWATI Enny Suswati Erfan Efendi Erma Sulistyaningsih Fitri, Nadya Eka Gama Wisnu Sanjaya Habib Mustofa Habibbur Rochman Salim Habibi, Ali Hamzah, Salsabilla Maula Zalfa El Hanifa Rizki A.S. Hibatulloh, Muhammad Farhan Hidayat Hidayat Himma, Alyatul I Nyoman Semita Iftinan, Karenzha Ika Rahmawati Sutejo Intan Palupi Irawan Fajar Kusuma Jamalullail, Jamalullail Jauhani, Muhammad Afiful Jauhar Firdaus Kirana Nadyatara Kristianningrum Dian Sofiana Kusiyanto Taslim, Fitranda Laila Rizqi Kurniawati Lailatis Shofia Laksmi Indreswari M. Ali Shodikin Mei Liana Wati Miftakhul Huda Muhammad A. Shodikin Muhammad Ali Shodikin Muhammad Dimas Arya Candra Permana Mukhamad Najib Napitupulu , Frengki Nastiti Bekti Utami Nihayah Lukman Nisrina Salsabila Firmansyah Nita Alfianti Noorhamdani AS Noorhamdani AS Nugraha, Bima Setia Sandya Nurul Indah Saffanah Nurul Indah Saffanah Nurus Saffana Yulianto Pralampita, Pulong Wijang Primadana, Aditya Putra Uta, Diego Rafi Putra, Muhamad Dwi Eka Putra, Yudha Ananta Khaerul Ramadhani, Alfiyah Regina Finka Rinda Yanuarisa Rony Prasetyo Rosita Dewi Rustono - Ryan Ravi Is Syahputra S.Pd. M Kes I Ketut Sudiana . Sa'adah, Ajeng Samrotu Sakinah, Elly N Salsabila, Yuna Annisa Salsabilla Maula Zalfa El Hamzah Saputra, Antonius Dwi Sarah Marsa Tamimi Semita, I Nyoman septa surya wahyudi, septa surya Setyawardani, Astuti Sheilla Rachmania, Sheilla Shodikin, M. Ali Shofia, Lailatis Siti Marissa Aisyah Siti Zulaikha Sumarno Retoprawiro Supangat Supangat Syahputri, Siti Ananda Hardita Tristianti, Farmitalia Nisa Udi Rusadi Ulfa Elfiah Ulin Nuha Jazmin Widaningsih, Titi Widhiasari Normaningtyas Wiwien Sugih Utami Yudha Nurdian Yuli Hermansyah Yuli Rahmantia, Vania Yuna Annisa Salsabila Yunita Armiyanti

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