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All Journal Bioscience Pendidikan Biologi Biocelebes Jurnal Penelitian Pendidikan IPA (JPPIPA) Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Biologi Tropis Jurnal Agrotek Tropika JURNAL PENDIDIKAN TAMBUSAI FONDATIA AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Pelita Eksakta Sainstek : Jurnal Sains dan Teknologi Jurnal Abdi Insani Journal On Teacher Education (Jote) Al Jahiz: Journal of Biology Education Research MASALIQ: Jurnal Pendidikan dan Sains Jurnal Serambi Biologi
Irdawati Irdawati
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary Other

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Potency of Yeast from Orange Peel’s Ecoenzyme as Antimicrobe Elsa Sri Handayani; Dezi Handayani; Irdawati Irdawati; Violota Violita
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Orange peels can still be utilized even though they are categorized as waste. Orange peels still have quite a lot of nutrients and contain various active compounds. Orange peels can be used as a source of organic material for the manufacture of ecoenzyme. The ecoenzyme produced from orange peel has a distinctive and fragrant aroma so that it is preferred compared to ecoenzyme made from other organic materials. During the ecoenzyme fermentation process, there are various types of bacteria and fungi that grow, but their roles are not yet known. The results of previous research obtained eight yeast isolates from the fermentation process of orange peel ecoenzyme, but only six isolates can be tested because two isolates are contaminated. The purpose of this study was to see the potential of yeast from citrus peel ecoenzyme as an antimicrobial. The antimicrobial activity test method used was the agar solid diffusion test. The presence of antimicrobial activity is characterized by a zone of inhibition around the colony of yeast isolates. The results showed that all isolates of yeast from orange peels ecoenzyme were able to inhibit the growth of E. coli and only five isolates were able to inhibit the growth of S. aureus, but none were able to inhibit C. albicans. The diameter of the inhibition zone against E. coli ranged from 8.3 mm to 17.5 mm while S. aureus ranged from 7.8 mm to 12.9 mm. Therefore, yeast derived from orange peel ecoenzyme has potential as an antimicrobial, especially against E. coli. and S. aureus.
ANALYSIS SPECIFIC ACTIVITY OF XYLANASE ENZYME PRODUCED BY THE CONSORTIUM OF THERMOPHILIC BACTERIA FROM SAPAN SUNGAI ARO HOT SPRING Feby Yeriska; Mulia; Irdawati; Linda Advinda; Dwi Hilda Putri
Jurnal Serambi Biologi Vol. 8 No. 3 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Thermophilic bacteria are bacteria that are able to survive in high temperatures ranging from 45 – 80oC so that they have the potential to produce enzymes that are stable against heat. Thermophilic bacteria can be found in various places in nature that have potential hot springs. One of the potentials of thermophilic bacteria in producing enzymes is the xylanase enzyme. Xylanase is able to hydrolyze xylan (hemicellulose) into xylo-olisaccharides and xylose. In the industrial world, xylanase enzymes are used as paper whiteners, increase the volume of bread, as juice purification, and are used in the manufacture of detergents. This research is a type of descriptive research that aims to determine the specific activity of the xylanase enzyme and the levels of xylanase protein produced by thermophilic bacteria SSA (Sapan Sungai Aro) with bacterial isolates SSA3, SSA4, SSA5, SSA6, SSA7, SSA8, SSA13, SSA14 and SSA15. The results showed that the bacterial isolates SSA3, SSA4, SSA5; isolates SSA6, SSA7, and SSA8; and SSA13, SSA14 and SSA15 respectively had specific activity of the xylanase enzyme with an average value of 0.1614 Unit/mg; 0.1638 Unit/mg and 0.1473 Unit/mg. The final measurement results showed that the highest specific activity of the xylanase enzyme was in the consortium of thermophilic bacteria isolates SSA6, SSA7, and SSA8 with an average of 0.1638 Unit/mg.
PRODUCTION OF SPESIFIC XYLANASE ENZYME BY MUDIAK SAPAN HOT WATER THERMOPHILIC BACTERY Irdawati Irdawati; Muhammad Deedat Ayasy; Azwir Anhar; Linda Advinda; Yusrizal Yusrizal
Bioscience Vol 8, No 1 (2024): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/bsc.v8i1.122728

Abstract

Xylanase is an extracellular enzyme capable of hydrolyzing hemicellulose so that it can convert xylan into xylose. Thermostable xylanase enzymes can be produced by thermophilic bacteria. Thermophilic bacteria are used because they have advantages such as the ability to increase enzyme production in adjustable catalytic specifications. Bacteria are not only in a single form but also exist in a mixed form called a consortium. Compared with a single isolate, the performance of the consortium is better. Consortium is a mixture of microbial populations in the form of communities that have cooperative, commensal, and mutualistic relationships. This study aims to look at the cooperation between the consortium isolates and the consortium's ability to produce xylanase enzymes. This research is a descriptive study. The bacterial consortium is fermented in beechwood xylan medium. The results of this study were that MSS 11, MS 18, MS 16 consortium produced the highest xylanase enzyme activity, namely 12,887.Xilanase merupakan enzim ekstraseuler yang mampu menghidrolisis hemiselulosa sehingga dapat merubah xilan menjadi xilosa. Enzim xilanase termostabil dapat diproduksi oleh bakteri termofilik. Bakteri termofilik digunakan karena dapat memiliki keunggulan seperti aktivitas dan peningkatan spesifikasi katalisis yang dapat diatur. bakteri tidak hanya dalam bentuk tunggal namun juga ada dalam bentuk campuran yang disebut konsorsium. Dibandingkan dengan isolat tunggal, kinerja konsorsium lebih baik. Konsorsium merupakan campuran populasi mikroba dalambentuk komunitas yang mempunyai hubungan kooperatif, komensal, dan mutualitsik. Penelitian ini bertujuan untuk melihat kerjasama antar isolat konsorsium dan kemampuan konsorsium dalam menghasilkan enzim xylanase. Penelitian ini bersifat deskriptif. Pada penelitian ini, konsorsium bakteri difermentasikan dalam medium beechwood xylan. Hasil penelitian ini adalah konsorsium MSS 11, MS 18, MS 16 menghasilkan aktivitas enzim xylanase spesifik tertinggi yaitu 12,887.
ANALISIS PENGARUH TINGKAT FREE FATTY ACID (FFA) TERHADAP KUALITAS CRUDE PALM OIL (CPO) PADA PABRIK INDUSTRI KELAPA SAWIT PT. Agro Muko Po. Mill Yuandry, Syafrina; Irdawati, Irdawati
Biocelebes Vol. 18 No. 1 (2024)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v18i1.16882

Abstract

Indonesia merupakan salah satu negara yang memiliki potensi yang besar didalam sektor pertanian dan perkebunan, salah satu jenis tanaman yang saat ini dijadikan sebagai komoditas perkebunan yaitu kelapa sawit. Kelapa sawit merupakan salah satu komoditas perkebunan dan industri sebagai penghasil berbagai minyak seperti minyak industri, minyak masak, serta minyak bahan bakar atau biodiesel. Proses produksi nya dimulai dengan melakukan buah kelapa sawit sampai berubah menjadi Crude Palm Oil (CPO) atau minyak kelapa sawit. Salah satu parameter utama dalam menentukan kualitas dari CPO adalah kadar free fatty acid (FFA) yang terkandung didalam nya. FFA merupakan asam yang di bebaskan pada hidrolisa dari lemak yang ada pada kelapa sawit. Peningkatan kandungan FFA pada minyak kelapa sawit disebabkan oleh berbagai faktor salah satunya yaitu terjadinya kerusakan morfologi dan mikroorganisme pada buah kelapa sawit. Semakin tinggi presentase kandungan FFA pada CPO maka semakin rendah pula kualitas CPO tersebut, hal ini karena kadar FFA dapat menyebabkan banyak perubahan pada CPO. yaitu dapat menyebabkan ketengikan, perubahan rasa pada minyak, serta warna pada minyak yang dihasilkan. Selain itu tingginya kadar FFA didalam minyak kelapa sawit juga dapat menyebabkan menurunnya nilai jual pada CPO sehingga akan mengakibatkan kerugian perusahaan.
Variations in Alginate Concentration as a Material for Immobilizing Thermophilic Bacteria on the Characteristics of Beads Summaiati, Titi; Irdawati, Irdawati
Jurnal Serambi Biologi Vol. 9 No. 2 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i2.346

Abstract

Immobilization is a technique for physically confining or placing microbial cells in a certain space, where the cells still maintain catalytic activity. The cell immobilization technique aims to facilitate product purification, increase productivity, make it easier to control cell stability and microbial cells can be used repeatedly. One carrier that is often used as a matrix to trap microorganism cells is alginate. Alginate is a type of polysaccharide obtained through extraction from brown algae. The immobilization technique with aginate cells was chosen because of its non-toxic nature, simple procedure for immobilization and cheap price for industrial applications. Alginate concentration affects the physical and chemical properties of the beads produced, such as size, shape and color of the beads. This study aims to compare variations in alginate concentration as a material for immobilizing thermophilic bacteria on the characteristics of the beads. This research is descriptive research. Beads are made with varying concentrations, namely 2%, 3%, 4%, 5% and 6% alginate. Data in the form of beads characteristics which include size, shape and color of beads. Based on research, the results obtained are that the higher the alginate concentration, the larger the diameter of the beads, the color of the beads will be more intense and the shape of the beads will tend to be round, and the beads will be sturdier, and vice versa.
Produksi Energi Listrik Bakteri Termofilik MS 12 sebagai Microbial Fuel Cell pada Substrat TMM (Termofilik Minimum Media) Aryeni Fatwah, Monika; Irdawati, Irdawati; Andrian, Richard
Jurnal Serambi Biologi Vol. 9 No. 2 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i2.347

Abstract

Energi listrik merupakan aspek penting dalam kehidupan sehari-hari, namun sumber daya untuk produksi energi ini semakin terbatas. Keterbatasan ini mendorong perlunya perhatian serius terhadap penggunaan energi. Pentingnya aspek ramah lingkungan dalam pembangkitan energi listrik menjadi sorotan utama, salah satunya produksi energi listrik menggunakan mikroorganisme sebagai biokatalisator atau biasa dikenal dengan Microbial Fuel Cell (MFC). Tujuan penelitian untuk mengetahui produksi energi listrik dengan memanfaatkan bakteri termofilik sebagai MFC pada substrat TMM. Penelitian dilakukan di Laboratorium Mikrobiologi, Departemen Biologi, FMIPA, UNP. Pada penelitian ini digunakan isolat bakteri termofilik MS 12 yang berasal dari sumber mata air panas Mudiak Sapan. Data dianalisis secara deskriptif dengan menampilkan grafik dan gambar. Hasil penelitian memperlihatkan produksi tegangan listrik isolat MS 12 mencapai nilai tertinggi yaitu 640.5 mV pada waktu 20 jam inkubasi.
Growth Pattern of Thermophilic Bacterial Isolate SSA-16 from Sapan Sungai Aro Hot Springs Suherman, Donny; Irdawati, Irdawati
Jurnal Serambi Biologi Vol. 9 No. 2 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i2.350

Abstract

Thermophilic bacteria are a group of microorganisms that grow optimally at temperatures of more than 45°C and the general growth range is between 45°C to 80°C. To determine the growth pattern of thermophilic bacteria originating from the Sapan Sungai Aro hot springs, isolate SSA-16 was carried out by measuring the level of bacterial turbidity. The method used in this research is a descriptive approach using two replications (duplo). The isolate that is the focus is SSA-16. Bacteria were incubated for 24 hours at 2 hour intervals at a temperature of 50ºC and their growth was measured using a UV-Vis Spectrophotometer with a wavelength of 600 nm. The OD value is measured on a scale of 0 to 2. From the results of the tests that have been carried out, you can see the results of the Optical Density (OD) test to see the growth pattern of SSA-16 bacterial isolates using a UV-Vis spectrophotometer (600 nm) through several phases. The first phase is the lag phase which occurs from the 2nd to the 6th hour, the second is the exponential phase which occurs from the 8th to the 14th hour and reaches the highest average of 0.706 and in the next hour the bacterial growth is at the Stationary or bacterial growth can be declared stagnant.
Karakteristik Pola Pertumbuhan Bakteri Termofilik Isolat MS-12 dari Sumber Air Padas Mudiak Sapan Uthami, Feranis Nadia; Irdawati, Irdawati
MASALIQ Vol 4 No 1 (2024): JANUARI
Publisher : Lembaga Yasin AlSys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/masaliq.v4i1.2621

Abstract

The growth pattern of thermophilic bacteria can be observed through turbidity testing, representing each growth phase: lag, exponential, stationary, and death phases. To evaluate and understand the growth pattern of thermophilic bacteria, particularly MS-12 isolate from the Mudiak Sapan (MS) hot springs, it can be analyzed through Optical Density (OD) testing using a UV-Vis spectrophotometer (600 nm). The OD test is commonly employed to determine the turbidity level in a solution by measuring the light passing through the solution, proportional to the number of cells or particles within it. The testing method used in this study is descriptive with two replications. The research was conducted at the Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Padang State University on December 9, 2023. Bacteria were incubated for 12 hours at a temperature of 50ºC, and their growth was measured using a UV-Vis spectrophotometer with a wavelength of 600 nm. Turbidity measurements were taken every 2 hours during the 12-hour incubation period. The growth pattern of MS-12 bacterial isolate, as observed from the measurements, can be considered favorable. At the 2-hour measurement in the second hour, bacterial growth averaged at 0.1535 (lag phase). Measurements from the 3rd to the 8th hour reached an average of 0.8105 (exponential phase). The 9th and 10th hours exhibited a stationary phase, while the measurements in the last 2 hours indicated a death phase, signifying a decline in the bacterial growth pattern.
Produksi Biolistrik dengan Microbial Fuel Cell (MFC) dari Bakteri Termofilik Vestimarta, Aldi Wahyuda; Irdawati, Irdawati
MASALIQ Vol 4 No 1 (2024): JANUARI
Publisher : Lembaga Yasin AlSys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/masaliq.v4i1.2632

Abstract

The current availability of electrical energy is not proportional to the large demand. This emphasizes the importance of saving electrical energy. To replace fossil fuel power plants that cannot be renewed. So Microbial Fuel Cells (MFC) is used, which is a technology that is able to convert chemical energy from organic matter into electrical energy through the process of oxidation and metabolism of anaerobic bacteria in electroactive biofilms at the anode. This study aims to determine the production of bioelectricity with Microbial Fuel Cell using consortium thermophilic bacteria on TMM substrate. This research was conducted in November 2023 in the microbiology laboratory, FMIPA, UNP. Data analysis was carried out descriptively by displaying images and graphs. Observation of the development of SSA 16 thermophilic bacteria was carried out for 24 hours by checking the voltage once every 2 hours. And the highest voltage result was 749 mv at the 10th hour of measurement.
Isolasi dan Karakterisasi Bakteri Asam Laktat (BAL) pada Fermentasi Durian Montong (Durio zibethinus Murr.) Viona, Alda; Fevria, Resti; Irdawati, Irdawati; Putri, Dwi Hilda
MASALIQ Vol 4 No 1 (2024): JANUARI
Publisher : Lembaga Yasin AlSys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/masaliq.v4i1.2636

Abstract

Lactic acid bacteria is a group of bacteria that produce lactic acid as the main product in fermentation. Tempoyak is a processed durian fruit product made by anaerobic spontaneous fermentation for 3-7 days. The fermentation process that occurs in making tempoyak is lactic acid fermentation. Carbohydrates are broken down into glucose, then BAL will ferment the glucose to produce lactic acid (main product), ethanol and CO2 (by-product). The aim of this research was to isolate lactic acid bacteria (LAB) from fermented durian. This research method is descriptive. 8 BAL isolates were obtained and identified macroscopically and microscopically by gram staining method. Based on the research that has been done, colonies of gram-positive bacteria in the form of bacilli and coccus cells were obtained. Gram-positive bacteria have cell wall characteristics with thicker peptidoglycan so that color absorption from violet crystals absorbed in cells will survive.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abror Abror Agustien Agustien Ahmad, Nurafni Fajarna Do Andrian, Richard Anthoni Agustien Apriansa, Apriansa Ardi Ardi Armiliandi, Rada Aryeni Fatwah, Monika Az-Zahra, Fauziah Azwir Anhar Bob Refianto Deni Kurniati Des M Des Desi Nurmayenti Dezi Handayani Dezi Handayani Dwi Hilda Putri Dwi Khayrun Nissha Elfah rahma Elsa Sri Handayani Eria Marina Sepriyani Fahra Fahra Fahra Fahra Fajri, Dilham Hidayatul Faradilla Rattriana Fathma Dwi Fatiha Fauzana Ahmad Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Francantika, Manja Ghiffari, Muhammad Gustina Indriati Heffi Alberida Helendra Helpida Helpida Ilsa Septia Putri Inayatul Fatia Indrawani Matondang Irma Leilani Jannah Koftiah Khalif Alfajri Kurnia Wulansari Larashinda, Mentari Linda Advinda Lufri Lufri Luthfi, Afdal Mades Fifendy Marissa Angelina Maulina, Adinda Rizky Mei Niarti Melsi Pratiwi Yusni Monhartini Monhartini Moralita Chatri Muhamad Sholichin Muhammad Aulia Rahman Muhammad Deedat Ayasy Muhyiatul Fadilah Mulia Mulia Mulia Mulyadi, Rival Muskhir, Mukhlidi Nada Nafion Nia Ramadhanti Nisa Afifah Novicka Putri Anggraeni Nur Shofiatun Nisa Nur Vaizi Nurfatihah Z, Zahara Oksa Afdarina Putri Andriani Putri Ardila Sari Putri Rachma Auliya Putri Rahma Padilla Putri Sarinami Rahmi, Elva Refni Syahleli Afni Rizka Putri Alti Roza Yolanda Sasmeri Sasmeri Selaras, Ganda Hijrah Sri Wahyuni Suci Fajrina Suci Fajrina Suherman, Donny Summaiati, Titi Syahrastani, Syahrastani Syamsuardi Syamsuardi Syamsurizal S Threesia Anugrah Ilahi Tomi Apra Santosa Uthami, Feranis Nadia Vauzia Vauzia Vestimarta, Aldi Wahyuda Violeta Violita Violita Violita Violita Viona, Alda Wahyuda Vestimarta, Aldi Wardah Hayati, Wardah Wella Antika Weni Yulastri Windi Nopitasari Wirda Taufik Witri Nofita Safitri Y Rilda Yetria Rilda Yuandry, Syafrina Yuni Ahda Yusrizal Y Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zulyusri, Zulyusri