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All Journal Bioscience Pendidikan Biologi Biocelebes Jurnal Penelitian Pendidikan IPA (JPPIPA) Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Biologi Tropis Jurnal Agrotek Tropika JURNAL PENDIDIKAN TAMBUSAI FONDATIA AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Serambi Biologi Pelita Eksakta Sainstek : Jurnal Sains dan Teknologi Jurnal Abdi Insani Journal On Teacher Education (Jote) International Journal of Education and Literature (IJEL) MASALIQ: Jurnal Pendidikan dan Sains Jurnal Serambi Biologi
Irdawati Irdawati
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Veterinary Other

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The Potential of Hot Water Mudiak Sapan Thermophilic Bactery Consortium Formulation in Producing Xylanase Enzyme Irdawati; Indrawani Matondang; Linda Advinda; Azwir Anhar; Yusrizal Y
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5309

Abstract

The xylanase enzyme has high commercial value in the industrial sector, including the food industry, animal feed, bleaching of pulp/pulp, lignocellulosic bioconversion as fuel, and in the food industry, namely the cheese, bread and meat industries, while in the non-food industry this can be used in detergents. Xylanase enzymes can be produced from microorganisms, one of which is thermophilic bacteria. Thermophilic bacteria are known to be able to produce thermostable enzymes and proteins that are heat resistant. Xylanase production in compatible bacterial consortia was higher than monoculture. The purpose of this study was to determine the bicultur consortium that has the potential to produce xylanase enzymes. This research is an experimental research. Enzyme activity testing used the Miller method with DNS (Dinitrosalycilic acid) reagent using a completely randomized design (CRD) with 3 repetitions. Data on the results of xylanase activity were analyzed by ANOVA test and DMRT follow-up test with a 5% significance level.The results of the thermophilic bacterial isolates in the consortium that have the potential to produce xylanase enzymes are MS18 & MSS15,  MSS15, MS16 and MS18 & MS16.
Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production Irdawati Irdawati; Fahra Fahra; Dwi Hilda Putri; Dezi Handayani; Yusrizal Yusrizal
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.3597

Abstract

Biofuels are fuels derived from biomass and consist of biogas, biodiesel and bioethanol. Bioethanol is a biofuel whose main ingredients are from plants and generally use microorganisms in the fermentation process. One way is to use thermophilic bacteria with the advantage of low contamination levels and better product quality. Bacteria that exist in nature are not only in a single form but also exist in the form of a consortium in which there are beneficial or detrimental bacterial interactions. Favorable bacterial interactions indicate that the bacteria are compatible. Using a consortium with compatible bacteria gives better results than using a single bacteria. This study aims to determine the compatibility and effect of a consortium of thermophilic bacteria from Mudiak Sapan hot springs on biofuel yields. This research is a descriptive research. To test the cooperation between isolates, a compatibility test was carried out using the disk diffusion method. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%.
The Validity and Practicality of SSCS-Based Student Worksheet on Ecology Material and Environmental Change Syamsurizal; Irdawati; Violita; Refni Syahleli Afni; Muhamad Sholichin
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4444

Abstract

This research aims to produce student worksheet based on SSCS (Search, Solve, Create and Share). This type of research is development research using the Plomp model. The development stage of this model starts from the initial investigation stage, the development or prototyping stage, and the assessment stage. The instruments used in this development research were teacher questionnaire sheets, student response questionnaire sheets, self-evaluation sheets, and SSCS-based LKPD validation sheets (Search, Solve, Create and Share) by experts, as well as SSCS-based LKPD practicality assessment sheets (Search, Solve, Create and Share) which is filled in by teachers and students. The results showed that the SSCS-based LKPD (Search, Solve, Create and Share) was very valid with a score of 86.31%. The practicality assessment of student assessment in field trials (group evaluation) with a score of 92.00% with very practical criteria, and the practicality assessment by the teacher obtained a score of 96.00% with very practical criteria. It can be concluded that the LKPD based on SSCS (Search, Solve, Create and Share) that has been developed is included in the category of very valid and very practical.
Implementation of the Problem Based Learning Model to Increase the Activity and Learning Outcomes of Students in the Digestive System Material and the Human Respiratory System Class XI MAN 2 Tanah Datar Sri Wahyuni; Syamsurizal; Irdawati; Violeta; Suci Fajrina
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4537

Abstract

This study aims to determine the effect of the model Problem based Learning in  improve results and learning activities in biology learning system material digestion and the human respiratory system. The type of research carried out is in accordance with the problem researched is study action class (Classroom action research). The population in this study were students of class XI MIPA MAN 2 Tanah Datar who were enrolled in the 2022/2023 academic year. The sample in this study were students in class XI MIPA 1 and XI MIPA 2 MAN 2 Tanah Datar. Data collection techniques used in this study were observation, questionnaires, documentation and field notes. The data analysis technique used to analyze activity data and student learning outcomes is the percentage technique and the t test. The results of this study indicate that the average student learning outcomes have increased from cycle I (68.55 %) to cycle II (86.20%). In line with the learning activities of students also increased from cycle I (48 %) to cycle II (77%). The conclusion of this study is that the Problem Based Learning (PBL) learning model can increase the activity and learning outcomes of students in biology class XI IPA MAN 2 Tanah Datar.
POTENTIAL OF THERMOPHIC BACTERIAL XILANASE AS ENVIRONMENTALLY FRIENDLY FABRIC BLEACH Nur Shofiatun Nisa; IRDAWATI IRDAWATI; Dwi Hilda Putri; Dezi Handayani; Y Yusrizal
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The main conclusion contains what is the implication to other studies or to the future brought by the study. Xylanase is an enzyme that is widely used in industry because this enzyme can hydrolyze xylan (hemicellulose) into xylo-oligosaccharides and xylose. This enzyme can be produced by thermophilic bacteria. One of the benefits of xylanase is as an environmentally friendly fabric bleaching process (biobleaching). The use of xylanase enzymes can reduce the use of toxic chemicals such as chlorine by about 20-40%. The purpose of this study was to determine the effect of temperature and pH on xylanase production activity using rice straw xylan extract as a substrate and to see the potential of MS 18 thermophilic bacterial xylanase on fabric brightness. This research is a descriptive study with temperature and pH optimization treatment using 6 treatments and 3 replications, respectively. Enzyme activity was measured using a spectrophotometer with a wavelength of 540 nm. The data obtained are displayed in the form of tables and figures. The xylanase potential on the brightness level of the fabric was seen based on the results of the kappa number. The results of this study obtained the optimum temperature of xylanase activity at a temperature of 50℃ with the highest xylanase 3.792 U/mL. while at pH the optimum pH of xylanase activity was found at pH 7 with the highest xylanase production of 4.448 U/mL. The potential of xylanase on the brightness of the fabric based on the results of the kappa number is 2.04 lower than without using xylanase, which is 6.46.
Uji Daya Hambat Ekstrak Daun Gambir (Uncaria gambir Roxb.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus Putri Rahma Padilla; Mades Fifendy; Irdawati; Dezi Handayani
Jurnal Serambi Biologi Vol. 7 No. 3 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Staphylococcus aureus merupakan salah satu mikroflora normal yang berada dalam mulut, apabila dipengaruhi oleh faktor predisposisi seperti perubahan kuantitas mikroorganisme menjadi tidak seimbang akan menimbulkan infeksi. Beberapa penyakit dalam rongga mulut dan sekitarnya yang dapat disebabkan oleh Staphylococcus aureus yaitu abses, ginggivitis, angular cheilitis, parotitis, staphyloccal mucositis dan denture stomatitis. Untuk mengatasi infeksi Staphylococcus aureus dapat digunakan suatu alternatif, salah satunya mencari senyawa-senyawa yang berpotensi sebagai antibiotik dari tumbuhan yang memiliki khasiat obat. Pengobatan untuk infeksi Staphylococcus aureus adalah dengan pemberian antibiotik yang dapat mengahambat pertumbuhan atau mematikan Staphylococcus aureus yang menginfeksi. Salah satu tanaman yang dapat dimafaatkan sebagai obat tradisional sebagai antibiotik adalah daun gambir (Uncaria gambir). skrining fitokimia dari ekstrak daun gambir menunjukan terdapatnya kandungan golongan senyawa kimia di dalam simplisia dan ekstrak etanol gambir yaitu alkaloid, flavonoid, saponin, dan tannin yang mempunyai sfektivitas sebagai antioksidan. Penelitian ini merupakan penelitian eksperimen, menggunakan metode RAL yang terdiri dari 6 perlakuan dan 3 pengulangan dengan pemberian ekstrak daun gambir konsentrasi 10%, 20%, 30%, 40%, 50% serta kontrol positif (Amoxicilin 10%). Hasil penelitian menunjukkan bahwa ekstrak daun gambir mampu menghambat pertumbuhan bakteri Staphylococcus aureus. Konsentrasi optimum ekstrak daun gambir dalam menghambat pertumbuhan bakteri Staphylococcus aureus pada konsentrasi 50% sebesar 21,6 mm.
Compatibility Test of the Biculture Consortium of Thermophilic Bacteria from Mudiak Sapan Hot Fahra Fahra; Irdawati Irdawati
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Thermophilic bacteria are bacteria that are resistant to hot temperatures and have several other advantages in producing industrial fields. Bacteria also exist in the form of a mixture (consortium) where the bacteria work together to produce enzymes. This study aims to examine the compatibility of thermophilic bacterial isolates. This research is descriptive in nature using the Spread Plate Method. The results showed that the thermophilic bacterial isolates tested (MS 9, MS 12, MS 18 and MS 17) and all their compatible partners were seen with no inhibition zones. Keywords : Biofuel, thermophilic bacteria, compatibility test.
Endophytic Fungi from Simpei Fern Rhizomes and Its Phosphate Solubilization Activity Windi Nopitasari; Dezi Handayani; Linda Advinda; Irdawati Irdawati
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Phosphate is very abundant in the soil, but only a small amount was dissolved. Some fungi have the ability to dissolve phosphates. This fungi is expected to overcome the excessive use of phosphate fertilizers. Those fungi ussualy found in soil or rhizorfere and sometimes they are endophytic. Rhizome of the simpei fern has endophytic fungi and its phosphate solubilizing activity was not known yet. This study aims to isolate endophytic fungi from rhizome of the simpei fern and to determine its phosphate solubilizing activity. Samples of simpei fern rhizome were taken from Nagari Aka Barayun, Harau District, Lima Puluh Kota District, West Sumatra. Surface sterilization using 0.5% NaOCL. The isolation medium for endophytic fungi used PDA medium and the phosphate solvent activity test used Pikovskaya medium. Six isolates of endophytic fungi were successfully isolated from the rhizome of the simpei fern and only three isolates were able to dissolve phosphate. The isolates are isolates R4, R5, and R6. Phosphate solubilizing activity of isolate R5 was higher than other isolates, although all isolates had a low phosphate solubility index.
Isolation of Phosphate-Solubilizing Fungi from Forest Soils of the Harau Valley Roza Yolanda; Dezi Handayani; Azwir Anhar; Irdawati Irdawati
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The decline in the quality of agricultural products due to nutrient deficiencies such as phosphate is a major problem for agriculture in Indonesia. Technological advances have produced a way to overcome phosphate availability, namely by using phosphate-solubilizing fungi. Organic acids produced by phosphate-solubilizing fungi help reduce phosphate binding in the soil. This study aims to determine the activity of phosphate-solubilizing fungi isolated from Harau Valley Forest soil. Soil sampling was done by the purposive sampling method. The sampling area was along the Aka Barayun Forest in the Harau Valley. Isolation of fungi was carried out using the multilevel dilution method and poured on PDA medium. The fungus activity test was carried out using Pikovskaya media. Based on the results of the study, 6 isolates were obtained, 3 of which had phosphate solubilizing activity, as indicated by the formation of a clear zone around the fungal colonies. Fungal isolates were measured for colony zone diameter, clear zone diameter, and phosphate solubility index (IKF). The isolate with the highest activity was isolate T1, with an IKF of 1.0. The ability to dissolve phosphate of the fungal isolates obtained was categorized as low.
Isolation of Endophytic Fungi from Trichoma of the Simpei Fern (Cibotium barometz (L.) J. Sm) and Phosphate Solubilizing Activity Test Nia Ramadhanti; Dezi Handayani; Azwir Anhar; Irdawati
Jurnal Serambi Biologi Vol. 8 No. 2 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The problem that is often faced by farmers in West Sumatra is the decreasing level of soil fertility which has an impact on crop production due to a lack of phosphate uptake by plants. The problem of phosphate availability can be overcome by utilizing phosphate solubilizing fungi (CPF) because they can be used as biofertilizers. CPF is usually isolated from the soil or rhizosphere, but this fungus is not necessarily compatible with the host plant, so the isolation of endophytic fungi, especially indigenous fungi, is better because it can provide phosphate. CPF can be isolated from plant tissues such as trichomes. The aim of this study was to isolate endophytic fungi from chimpanzee fern trichomes and test their phosphate solubilizing activity. Samples were obtained from Nagari Aka Barayun, Harau District, Fifty Cities District, West Sumatra. Isolation of fungi using Potato Dextrose Agar (PDA) medium, phosphate solvent activity test using Pikovskaya medium. The results showed that 5 isolates of endophytic fungi were successfully isolated from chimpanzee fern trichomes with different macroscopic morphologies: spherical shape, various colors, cotton-like texture, smooth surface. Phosphate solubilizing activity test showed that only ETK 4 isolate had phosphate solubilizing activity with the highest IKF of 0.7.
Co-Authors Abdul Razak Abdul Razak Abdul Razak Abror Abror Agustien Agustien Ahmad, Nurafni Fajarna Do Andrian, Richard Anggraeni, Novicka Putri Anthoni Agustien Apriansa, Apriansa Ardi Ardi Armiliandi, Rada Aryeni Fatwah, Monika Az-Zahra, Fauziah Azwir Anhar Bob Refianto Deni Kurniati Des M Des Desi Nurmayenti Dezi Handayani Dezi Handayani Dwi Hilda Putri Elfah rahma Elsa Sri Handayani Fahra Fahra Fahra Fahra Fahra, Fahra Fajri, Dilham Hidayatul FAJRINA, SUCI Faradilla Rattriana Fatiha, Fathma Dwi Fauzana Ahmad Feby Yeriska Fevria, Resti Fitri Angraini Fitri Arsih Francantika, Manja Ghiffari, Muhammad Gustina Indriati Heffi Alberida Helpida Helpida Ilsa Septia Putri Inayatul Fatia Indrawani Matondang Irma Leilani Jannah Koftiah Khalif Alfajri Kurnia Wulansari Larashinda, Mentari Linda Advinda Lufri Lufri Luthfi, Afdal Mades Fifendy Marissa Angelina Maulina, Adinda Rizky Mei Niarti Melsi Pratiwi Yusni Monhartini, Monhartini Moralita Chatri Muhamad Sholichin Muhamad Sholichin Muhammad Aulia Rahman Muhammad Deedat Ayasy Muhyiatul Fadilah Mulia Mulia Mulia Mulyadi, Rival Muskhir, Mukhlidi Nada Nafion Nia Ramadhanti Nisa Afifah Nissha, Dwi Khayrun Nur Shofiatun Nisa Nur Vaizi Nurfatihah Z, Zahara Putri Andriani Putri Ardila Sari Putri Rachma Auliya Putri Rahma Padilla Putri Sarinami Rahmi, Elva Refni Syahleli Afni Rizka Putri Alti Roza Yolanda Sasmeri Sasmeri Selaras, Ganda Hijrah Sri Wahyuni Suci Fajrina Suherman, Donny Summaiati, Titi Syahrastani, Syahrastani Syamsuardi Syamsuardi Syamsurizal S Tomi Apra Santosa Uthami, Feranis Nadia Vauzia Vauzia Vestimarta, Aldi Wahyuda Violeta Violita Violita Violita Violita Viona, Alda Wahyuda Vestimarta, Aldi Wardah Hayati, Wardah Wella Antika Weni Yulastri Windi Nopitasari Wirda Taufik Witri Nofita Safitri Wulansari, Kurnia Y Rilda Y Yusrizal Yetria Rilda Yuandry, Syafrina Yusrizal Y Yusrizal Y Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Yusrizal Zakri, Dwika Febriana Zulyusri, Zulyusri