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All Journal PROSIDING SEMINAR NASIONAL BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Pro Food Jurnal Farmasi Sains dan Komunitas Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pengabdian Pada Masyarakat Indonesian Journal of Chemistry Jurnal Kesehatan Prima Gorontalo Journal of Public Health INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Jurnal Pengabdian Masyarakat Indonesia Majalah Ilmiah UPI YPTK Research Fair Unisri JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Buletin Pengabdian Prosiding Seminar Nasional Unimus
Stalis Norma Ethica
Universitas Muhammadiyah Semarang
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Journal : Pro Food

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KARAKTERISASI BAKTERI LIPOLITIK Bacillus sp. PADA WADI ORGAN PENCERNAAN IKAN SIDAT (Anguilla sp.): Characterization of Lipolitic Bacteria Bacillus sp. in Wadi of Digestive Organs of Sidat Fish (Anguilla sp.) Vicky Mahendra Nurzhulian; Ayu Rahmawati Sulistyaningtyas; Stalis Norma Ethica
Pro Food Vol. 7 No. 2 (2021): Pro Food (Jurnal Ilmu dan Teknologi Pangan)
Publisher : Fakultas Teknologi Pangan dan Agroindustri, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/profood.v7i2.205

Abstract

ABSTRACT One of the leading enzymes having the potential to donate profit of billions of dollars in food and health sector is lipase. Lipase can be produced from microorganisms including bacteria. Lipolytic bacteria can be found in the digestive tract of fish. This study aims to obtain lipolytic bacteria ifrom the wadi fermentation products of digestive organs of Sidat Fish (Anguilla sp.) and to identify bacterial species based on the 16S rRNA gene sequence. Samples of wadi fermentation products of fish digestive organs were serially diluted and cultured on Nutrient Agar (NA). The purified bacterial isolates were microscopically identified and tested for their lipolytic activity using Tween-80 agar media. Bacterial isolates with the highest lipolytic index were subjected to bacterial identification based on the 16S rRNA gene using the Polymerase Chain Reaction (PCR) method. The results showed that from the prepared wadi samples, five bacterial isolates coded WFAD-1 to WFAD-5 (WFAD stands for Wadi Fermentation of Anguilla sp. Digestive organs) could be obtained. Of these 5 isolates, two of them, WFAD-1 and WFAD-3, were capable of producing lipase and in particular, WFAD-1 had the highest lipolytic index of 2.95. BLAST analysis result on the amplified 16S rRNA gene of WFAD-1 isolate revealed a similarity level 85.79% and query coverage of 61% with Bacillus velezensis. Based on microscopic and molecular identification results, the lipolytic isolate WFAD-1 can be categorized into the genus Bacillus. As conclusion, Bacillus sp. WFAD-1 isolated from wadi fermentation product of digestive organs of ikan sidat has a potential as a source of bacterial lipase. ABSTRAK Salah satu enzim utama berpotensi menyumbang keuntungan miliaran rupiah di bidang pangan dan kesehatan adalah lipase. Lipase dapat dihasilkan dari mikroorganisme termasuk bakteri. Bakteri lipolitik dapat ditemukan di saluran pencernaan ikan. Penelitian ini bertujuan untuk mendapatkan bakteri lipolitik pada produk fermentasi wadi organ pencernaan ikan Sidat (Anguilla sp.) dan mengidentifikasi spesies bakteri tersebut berdasarkan urutan gen 16S rRNA. Sampel hasil fermentasi wadi organ pencernaan sidat diencerkan bertingkat lalu dikultur pada media Nutrient Agar (NA). Isolat bakteri yang telah dimurnikan diidentifikasi secara mikroskopis dan diuji aktivitas lipolitiknya menggunakan media agar Tween-80. Isolat bakteri dengan indeks lipolitik tertinggi diidentifikasi berdasarkan gen 16S rRNA dengan metode Polymerase Chain Reaction (PCR). Hasil penelitian menunjukkan bahwa dari sampel wadi yang telah dibuat, dapat diperoleh lima isolat bakteri yang diberi kode WFAD-1 hingga WFAD-5 (WFAD singkatan dari Wadi Fermentation of Anguilla sp. Digestive organ). Dari 5 isolat tersebut, dua di antaranya yaitu WFAD-1 dan WFAD-3 mampu menghasilkan lipase dan khususnya WFAD-1 memiliki indeks lipolitik tertinggi yaitu 2,95. Hasil analisis BLAST pada produk amplifikasi gen 16S rRNA isolat WFAD-1 menunjukkan tingkat kemiripan 85,79% dan cakupan query 61% dengan Bacillus velezensis. Berdasarkan hasil identifikasi mikroskopis dan molekuler, WFAD-1 lipolitik dapat dikategorikan dalam genus Bacillus. Sebagai kesimpulan, Bacillus sp. WFAD-1 yang diisolasi dari produk fermentasi wadi organ pencernaan ikan sidat berpotensi sebagai sumber lipase bakterial.  
Co-Authors A.A. Ketut Agung Cahyawan W Abdillah, Rifqi Aditya Rahman Ernanto Aditya Rahman Ernanto Aditya Rahman Ernanto Afriansyah, M. Ardi Afriansyah, Muhammad Ardi Agus Sabdono Agus Suyanto Agus Suyanto Aida Zakiyatul Fikriyah Ainutajriani Ainutajriani Ainutajriani, Ainutajriani Alisha Triwahyuni Alvira Intar Bella Cahya Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Ana Hidayati Mukaromah Andri Sukeksi Aprianti, Nurannisa Fitria Aprilia Indra Kartika, Aprilia Indra Arfiyanti, Mega Pandu Arham, Chaerul Aufit Fahima Aulia Harun Aulia Meirizka Saputra Ayanti, Bio Putri Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Ayu Rahmawati Sulistyaningtyas Cendani, Tinezia Rivan Dewi Seswita Zilda Dhea Ayu Lestari Dian Purwo Jatinging Putri Diatri, Devita Dilin Rahayu Nataningtyas Diyanah, Dina Dwi Pamaya Elly Rustanti Ellyka Purwaningrum Endang Semiarti Endang Semiarti Endang Tri Wahyuni Maharani Ernanto, Aditya Rahman Fanda Indriyani Fandhi Adi Wardoyo Farabi, Meka Faizal Fauziyyah, Dinah Febrianti, Pepi Feri Feri Fitrah, Reza Fuad, Hayatun Hayatun Fuad Hikmah, Annisa Nurul Ika Dyah Kurniati Istini Istini Izza, Naimatul JAKA WIDADA Jaka Widada Janah, Inayah Qori Nur Jimat, Resi Tondho Jumardi, Muhamad Kazi Mohammad Zillur Rahman Khoirunisa, Asiva L. Hartanto Nugroho Lestari, Citra Rahayu Maya Dian Rakhmawatie Maya Linda Shafira Meturan, Dessy Marselina Meyliana, Adinda Mirza, Sa’if Revallindo Mudyawati Kamaruddin Muhammad Ardi Afriansyah Muhammad Ardi Afriansyah Mukarromah, Ana Hidayanti Musa, Susan Hasanah Mutia Srikandi Fitria Nanik Marfu'ati Ningsi, Ana Lestari Noverson Lidaya Nur Hidayah Nur Hidayah Nur Hidayati Nur Hidayati Nurcahyono Nurcahyono Nurcahyono Nurcahyono, Nurcahyono Nurrahman Nurrahman Nurrahman Nurrahman Nurrahman Nurrahman Oedjijono Oedjijono, Oedjijono Okta Yosiana Dewi Pratama, Aji Sukma Puji Lestari Putri, Vivi Vahtalava Radna Safitri Rafiyanto, Achmad Ragil Saptaningtyas Ragil Saptaningtyas Ragil Saptaningtyas, Ragil Richard David Silvestre Riswan Riswan Rivana Ariyadi Rosyida Azis Rizki Sakti Imam Muchlissin Samiroh Samiroh, Samiroh Setia Iriyanto Setia Iriyanto Silvestre, Richard David Siska Nurprihandayani Siti Aminah Siti Aminah Siti mutmainnah Sofyan Yosias Beslar Sri Darmawati Sri Darmawati Sri Darmawati Sri Elvira Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi Sri Sinto Dewi suardi suardi Sukowiyono Sukowiyono Sukowiyono Sukowiyono Sulistyaningtyas, Ayu Rahmawati Sultistyaningtyas, Ayu Rahmawati Tamimi, Iqbal Theresia Liyana Sejati Tri Joko Raharjo Tri Joko Raharjo Viandy, Amartya Desta Vicky Mahendra Nurzhulian Vradea Pramesta Wa Ode Inayatul Wahyuni, Arti Widyani, Dhiyananda Wikanastri Hersoelistyorini Wikanastri Hersoelistyorini Yuni Nurkuntari Zakiya, Roma Dhona Beauty