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savitri, Lisa
Universitas Kadiri
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PENGGALAKAN KEBIASAAN HIDUP BERSIH DAN SEHAT SEBAGAI UPAYA PENCEGAHAH PENYEBARAN COVID-19 DI KELURAHAN TAMANAN, KOTA KEDIRI Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya
Jurnal Abdimas Ilmiah Citra Bakti Vol. 4 No. 2 (2023)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v4i2.1598

Abstract

Wabah COVID-19 telah menjadi pandemi global yang memengaruhi banyak negara, termasuk Indonesia. Pemerintah dan lembaga kesehatan telah melaksanakan berbagai langkah untuk mencegah penyebaran COVID-19. Salah satu langkah penting adalah menggalakkan kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri. Pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman masyarakat tentang kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19. Metode yang digunakan dalam pengabdian masyarakat ini adalah pendekatan partisipatif dengan melibatkan masyarakat secara aktif dalam pengumpulan data dan pelaksanaan kegiatan. Kegiatan yang dilakukan meliputi penyuluhan, demonstrasi, dan penyediaan fasilitas sanitasi. Aktivitas ini diikuti oleh 50 ibu rumah tangga selama 3 bulan, terdiri dari tiga kegiatan utama penyuluhan kesehatan tentang pola hidup bersih dan sehat untuk mencegah penularan COVID-19. Data untuk penilaian kebiasaan hidup sehat menggunakan instrumen kuesioner dan checklist sebelum dan sesudah pelatihan. Pengetahuan, sikap dan psikomotor ibu rumah tangga dalam penyebaran penularan COVID-19 setelah diberikan pelatihan kebiasaan hidup sehat selama 3 bulan mengalami peningkatan. Penilaian dilakukan dengan membandingkan hasil pre-test dan post-test pelatihan peserta. Setelah mengikuti pelatihan, sebagian besar kemampuan peserta masuk kategori tinggi pada aspek pengetahuan (76%), aspek sikap (82%) dan aspek aspek psikomotor (92%). Berdasarkan hasil pengabdian ini, dapat disimpulkan bahwa penggalakan kebiasaan hidup bersih dan sehat dapat menjadi upaya efektif dalam pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri.
EDUKASI PENJAMINAN MUTU PEMBUATAN MEDIA MIKROBIOLOGI PADA PEMERIKSAAN SEDIAAN KOSMETIK DI PT. TRISZIE LAB INDONESIA Lisa Savitri; Nur Fahma Laili; Datin An Nisa Sukmawati; Syntia Tanu Juwita; Ester Lianawati Antoro; Ida Septika Wulansari; Juan Vega Mahardhika; Yosep Mosse
Jurnal Abdimas Ilmiah Citra Bakti Vol. 4 No. 3 (2023)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v4i3.1610

Abstract

Penjaminan mutu dalam pembuatan media mikrobiologi pada pemeriksaan sediaan kosmetik sangat penting dilakukan untuk memastikan hasil yang akurat dan berkualitas. Pemilihan bahan baku yang berkualitas, pengendalian proses produksi, dan pengujian kualitas produk jadi merupakan aspek-aspek penting dari penjaminan mutu dalam pembuatan media mikrobiologi. Secara deskriptif, hasil perhitungan instrumen menunjukkan bahwa 8 responden (40%) mengalami peningkatan tingkat pengetahuan mengenai penjaminan mutu pembuatan media pemeriksaan mikrobiologi pada sediaan kosmetik setelah diberikan edukasi, sedangkan 12 responden (60%) tidak mengalami perubahan tingkat pengetahuan. Tingkat pengetahuan responden sebelum dan sesudah edukasi dianalisis dengan menggunakan SPSS versi 23 untuk mengetahui signifikansi edukasi. Data diuji menggunakan uji Shapiro-Wilk dan hasilnya menunjukkan nilai sig. sebelum dan sesudah edukasi ? 0,05, menunjukkan bahwa data terdistribusi secara normal. Selanjutnya, data dianalisis menggunakan Wilcoxon Sign Rank Test dan nilai p sebesar 0,038 ? 0,05, yang menunjukkan adanya perbedaan signifikan pada tingkat pengetahuan sebelum dan sesudah edukasi. Pada tahap simulasi, mitra diberikan kesempatan untuk melakukan pembuatan media pemeriksaan mikrobiologi pada sediaan kosmetik. Hasilnya menunjukkan bahwa sebagian besar responden berhasil melakukan proses simulasi dengan benar. Hal ini diharapkan dapat menjadi gambaran dan dapat diaplikasikan langsung dalam proses produksi oleh mitra.
EDUKASI PEMBUATAN MEDIA MIKROBIOLOGI HALAL PADA PEMERIKSAAN SEDIAAN KOSMETIK DI PT. TRISZIE LAB INDONESIA Lisa Savitri; Fendy Prasetyawan; Syntia Tanu Juwita; Juan Vega Mahardhika; Yosep Mosse
Jurnal Abdimas Ilmiah Citra Bakti Vol. 5 No. 2 (2024)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v5i2.2480

Abstract

Teknologi canggih yang terus berkembang di era revolusi industri, mempermudah akses informasi melalui media sosial, majalah, dan koran, memungkinkan perluasan pengetahuan dan pemahaman agama Islam. Kehati-hatian dalam memilih produk, terutama dalam konteks agama Islam, menjadi penting, dan Lembaga Pengkajian Pangan Obat-obatan dan Kosmetika Majelis Ulama Indonesia (LPPOM MUI) memainkan peran kunci dalam menilai dan menetapkan kehalalan produk. Peraturan Pemerintah No 39 tahun 2021 dan Undang-undang No 33 Tahun 2014 menetapkan prosedur sertifikasi halal untuk berbagai produk, termasuk kosmetik. Industri kosmetik di Indonesia tumbuh pesat, didukung oleh populasi muda, pertumbuhan ekonomi, dan peran media sosial. Kosmetik halal tidak hanya mencakup riasan wajah, tetapi juga produk skincare. Alkohol, yang sering digunakan dalam kosmetik, perlu dipertimbangkan, dan fatwa MUI No 11 tahun 2009 menguraikan bahwa alkohol tidak hanya terdiri dari etanol, tetapi juga senyawa lain. Produk kosmetik yang mengandung bahan tidak halal, seperti sodium heparin dari babi, perlu dihindari. Program Pengabdian Masyarakat di Universitas Kadiri bertujuan meningkatkan pemahaman mitra, terutama PT. Triszie Lab Indonesia, dalam analisis mikrobiologi halal untuk kosmetik. Melalui metode pengajaran dan demonstrasi, kegiatan ini melibatkan evaluasi pengetahuan mitra sebelum dan sesudah pelatihan. Hasilnya menunjukkan peningkatan pengetahuan dalam konteks industri kosmetik halal, kesadaran konsumen terhadap produk halal dapat memengaruhi konsep merek secara menyeluruh.
Pemberian Monosodium Glutamat Selama Masa Organogenesis Meningkatkan Perkembangan Embrio Cacat pada Mencit Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.328

Abstract

Monosodium glutamate (MSG) biasanya berbentuk kristal halus dan berwarna putih. Senyawa MSG dibuat melalui proses fermentasi dari bahan dasar pati (gandum) dan gula molasses (tetes tebu) yang diberi nama sebagai garam natrium dan asam glutamat. Pemberian MSG selama induk mencit bunting bersifat embriotoksik. Penelitian ini bertujuan untuk mengetahui efek toksik MSG pada perkembangan embrio selama masa organogenesis. Penelitian pemberian MSG terhadap mencit dimulai pada kebuntingan hari ketujuh yaitu pada tahap organogenesis. Perlakuan dosis MSG yang diberikan adalah dosis 528 mg/kg BB, 696 mg/kg BB, dan 872 mg/kg BB. Peubah yang diamati dalam penelitian ini adalah jumlah fetus hidup atau mati dalam rahim pada kebuntingan hari ke-18, di samping morfologi fetus, bobot fetus dan perkembangan tulang rangka fetus. Hasil penelitian menunjukkan bahwa pemberian MSG yang dilakukan secara terus menerus sejak kebuntingan hari ke-7 hingga 16 membuat MSG tersebut masuk ke dalam tubuh induk mencit yang tidak memiliki kemampuan untuk menetralisir dan mendetokfikasi senyawa-senyawa kimia sehingga terakumulasi pada embrio mencit. Zat-zat tersebut mencapai embrio melalui pembuluh darah dan memengaruhi perkembangan fetus mencit. Penelitian ini menunjukkan bahwa ada pengaruh MSG terhadap perkembangan fetus mencit (Mus musculus) selama periode organogenesis di antaranya yaitu kecacatan pada mata (mikropthalmia dan anoftalmia), hidrosefalus ringan, jumlah metakarpal, jumlah metatarsal, dan ruas cervikal vertebrae tidak sama panjang. Abnormalitas perkembangan fetus mencit selama periode organogenesis meningkat sejalan dengan peningkatan dosis MSG yang diberikan dan bervariasi antar perlakuan. Semakin tinggi dosis MSG yang diberikan pada induk mencit bunting, maka semakin sedikit jumlah fetus yang hidup. Dapat disimpulkan bahwa MSG memengaruhi perkembangan fetus mencit selama masa organogenesis.
The Protective Effect of Kentut Leaf Extract (Paederia foetida L.) on Gastric Histopathology in Escherichia coli-Infected Sepsis Mice Model Savitri, Lisa; Kasimo, Elfred Rinaldo
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.227-233

Abstract

Sepsis, a severe medical condition, signifies the systemic immune response to infection, often leading to organ dysfunction and mortality. Escherichia coli is a significant contributor to sepsis cases, particularly in gastrointestinal disorders. This study aimed to investigate the histopathological changes in the gastric tissue of mice induced by Escherichia coli infection and evaluate the protective effects of kentut leaf extract (Paederia foetida L.). Histopathological analysis revealed distinct alterations in the gastric tissue among different treatment groups. While normal mouse treatment showed no significant changes, negative control (K-) and positive control (K+) groups exhibited inflammation and hyperemia of the gastric mucosa, characterized by necrosis, degeneration, and inflammatory cell infiltration. Treatment with kentut leaf extract (P1, P2, and P3) resulted in milder inflammation compared to controls, indicating a protective effect against gastric mucosal damage induced by Escherichia coli infection. This protective mechanism is attributed to the anti-inflammatory properties of saponins, flavonoids, and essential oils present in kentut leaf extract. These findings underscore the potential therapeutic benefits of kentut leaf extract in mitigating gastric mucosal injury associated with bacterial infection.
The Correlation Between Widal Diagnostic Test, Total Leukocyte Count, and Platelet Count in Suspected Typhoid Fever Patients at RS Aura Syifa Kediri Loviana, Novi; Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.555-558

Abstract

Typhoid fever is an acute systemic infectious disease that is related to poor personal hygiene and environmental sanitation. The diagnosis of typhoid fever is established through laboratory examinations, including serological, hematological, and bacteriological tests. This research aims to determine the correlation between the Widal diagnostic test and the total leukocyte count and platelet count in suspected typhoid fever patients at RS Aura Syifa Kediri. The study design used in this research is quantitative descriptive analysis with a cross-sectional approach. The data was collected retrospectively from secondary sources, specifically medical records of patients from August 1, 2021, to March 31, 2022, at RS Aura Syifa Kediri. The total population comprised 157 patients, and a purposive sampling method was used to select 41 patients who met the inclusion and exclusion criteria. The statistical analysis using the Spearman correlation test resulted in p-values ≥ α = 0.05 for the following correlations: Widal test O with leukocytes (0.538 ≥ 0.05), Widal test H with leukocytes (0.915 ≥ 0.05), Widal test O with platelets (0.476 ≥ 0.05), and Widal test H with platelets (0.965 ≥ 0.05). These findings indicate that there is no significant correlation between the Widal test O and H with the platelet count in patients with typhoid fever. Therefore, it can be concluded that there is no significant relationship between the Widal test O and H results and the platelet count in typhoid fever patients.
An Overview of The Total Leukocyte Count in Pulmonary Tuberculosis Patients at RSUD Gambiran Kediri City Fitri, Devi Nor; Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.671-674

Abstract

The leukocyte count examination in tuberculosis infection serves to monitor the inflammation caused by Mycobacterium tuberculosis. An increasing leukocyte count can lead to complications such as pleuritis. The purpose of this study was to determine the total leukocyte count of pulmonary tuberculosis patients at RSUD Gambiran, Kediri City, based on gender and age. This research was a descriptive retrospective study. Data analysis was conducted using Microsoft Excel and presented in the form of tables and diagrams. The results of this study showed that pulmonary tuberculosis infection was more prevalent in males, with 26 individuals (62%) affected. In terms of age groups, the highest infection rate was in the 21-60 years age range, with 30 individuals (71%) affected. As for leukocyte count elevation (leukocytosis), it was more commonly observed in males with a count of 14.57 µL. In terms of age groups, leukocytosis was frequently observed in the 21-60 years age range, with a count of 48.66 µL. Out of the 42 samples, 19 individuals had a normal leukocyte count, while the remaining 23 individuals experienced leukocytosis. From the results of the study, it can be concluded that not all pulmonary tuberculosis patients experience leukocytosis; some patients maintain a normal leukocyte count.
Testing Antibacterial Activity of Daun Kentut (Paederia foetida L.) Leaf Extract against Escherichia coli in Vitro Savitri, Lisa; Tiyas, Eka Wahyuning; Krissanjaya, Rochmad
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.277-283

Abstract

Escherichia coli is a pathogenic bacterium that causes infectious diseases in the digestive tract and can produce Lipopolysaccharide (LPS), including lipid A. Exposure to endotoxins in lipid A, can cause systemic effects, such as sepsis, which can lead to clinical manifestations and even death. Daun Kentut (Paederia foetida L.) is one of the herbal plants containing bioactive compounds that can inhibit the growth of E. coli bacteria. The aim of this research is to determine the effective concentration of daun kentut leaf extract to inhibit E. coli growth. This study is an experimental research using maceration extraction method and antibacterial disc diffusion method with 3 repetitions at concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, negative control (aquadest), and positive control (ciprofloxacin) conducted from March to April 2023 in the Microbiology Laboratory of Kadiri University. The observation results of inhibition zones in each treatment varied. The presence of these inhibition zones is due to the presence of secondary metabolite compounds in daun kentut leaf extract that have antibacterial activity, such as inhibiting protein synthesis mechanisms, causing damage to cell wall permeability, and ultimately leading to bacterial cell lysis and death. The statistical analysis using the One Way Anova test with a P-value of 0.05 shows that each variable has significant differences and effects. Thus, it can be concluded that the effective extract variation is the 100% concentration because, in this study, the 100% concentration has an inhibition zone of 26.72 mm and is classified as very strong in inhibiting E. coli bacteria.
In Silico BCell Epitope Design of Zika Virus Vaccine Using “Zika Virus Isolate Zika Virus/H sapiensTc/Tha/2006/CVD_06020 Complete Genome” Savitri, Lisa; Juwita, Syntia Tanu; Antoro, Ester Lianawati; Wulansari, Ida Septika; Sukmawati, Datin An Nisa; Freitas, João Gregório; Ornai, Andre Mario Sequeira Alves
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.525-531

Abstract

ZIKV has been found in the cerebrospinal fluid (CSF) and brains of adults infected with viruses that manifest neurological disorders. ZIKV is a mosquito-borne single-stranded RNA virus, which belongs to the family Flaviviridae. The efforts of the scientific community have rapidly increased knowledge about this virus. However, understanding the complexities of ZIKV infection, transmission and pathogenesis remains an urgent challenge. Therefore, it is critical to study competent vectors and natural reservoirs for ZIKV, viral genetic diversity and flavivirus coinfection. Due to the great challenges to develop a ZIKV vaccine, it is still not possible to be immunized against ZIKV infection and related pathologies. The methods are nucleotide search for the Zika virus was carried out in silico, using the NCBI bioinformatics application by providing access to biomedical and genomic information. /H. sapiens-tc/THA/2006/CVD_06-020, the complete genome was then searched for FASTA, then prediction of vaccine epitope using the IEDB. The vaccine candidate peptides were analyzed for their antigenicity using VaxiJen. Proteins were classified by AllerTop to known allergenicity, then ToxinPred to predict and design toxic/non-toxic peptides. There are 30 peptide sequences are predicted to be a candidate of peptides B-cell epitope zika virus vaccine design using “zika virus isolate zika virus/H. sapiens-tc/THA/2006/CVD_06-020, complete genome”.
Leaf Extract of Kentut (Paederia foetida L.) as a Preventive Measure Against Interleukin-6 Expression in the Liver of Mice in a Sepsis Model Injected with Escherichia coli Savitri, Lisa; Freitas, Maria Do Carmo Da Costa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.329-336

Abstract

Kentut leaves (Paederia foetida L.) are a medicinal plant that can be used as a preventative medicine against sepsis. This plant contains secondary metabolite compounds such as alkaloids, flavonoids, triterpenoids, saponins, and other active compounds. This research aims to to determine the influence and effective dosage of Kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in a sepsis model injected with E. coli. The method used was a Completely Randomized Design (CRD). The study involved 24 white male mice divided into 6 groups. Data analysis was performed using One way ANOVA. The average values of IL-6 expression in the mouse livers for each group are as follows: KN at 7.09%±0.06; K+ at 26.36%±0.02; K- at 72.60%±0.05; PI (100mg/kgBW) at 71.04%±0.04; PII (300mg/kgBW) at 62.22%±0.02; and PIII (500mg/kgBW) at 40.92%±0.01. The research results indicate an influence of kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in the sepsis model injected with E. coli, with a significance value of 0.000 or p-value < 0.005. The effective dosage of kentut leaf extract as a preventive measure against IL-6 expression is the PIII dosage of 500mg/kg BW. The anti-inflammatory mechanism in sepsis is thought to be caused by the presence of flavonoids, alkaloids, phenolic acids, and terpenoid compounds. The most likely anti-inflammatory mechanism is believed to involve flavonoids inhibiting cyclooxygenase (COX) and lipoxygenase (LOX) enzymes involved in the synthesis of inflammatory mediators such as prostaglandins and leukotrienes, which can trigger IL-6 production.
Co-Authors Afrilla, Rananda Agung Dwi Wahyu Widodo Agustanti, Khalimatus Nur Eka Alimansur, Moh Alves, Silvina Sandra X. Amanda, Cornelia Aminah Alfiani Ana Retnowati Anjarwati, Aprilia Antoro, Ester Lianawati Apriana, Dita Arif Budi Setiawan Besan, Emma Jayanti Daniati, Mila Putri Datin An Nisa Sukmawati Dhony Hermanto Edda, Ilda Rambu Eka Wahyuningtiyas Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo, Elfred Rinaldo Elly Megasari Ester Lianawati Antoro Ester Lianawati Antoro Ester Lianawati Antoro Ester Lianawati Antoro Evi Laela Sari Farabi, Meka Faizal Fendy Prasetyawan Fitri, Devi Nor Freitas, João Gregório Freitas, Maria Do Carmo Da Costa Herman Hilmi, Mochamad Hanif Hilmi, Mochammad Hanif Ida Septika Wulansari Ida Septika Wulansari Ida Septika Wulansari Ida Septika Wulansari Ihsan, Kharisul Indrayanti, Dian Iza Dwi Muslikha Juan Vega Mahardhika Juwita, Syntia Tanu Kadir, Mujtahid Bin Abd Kadir, Mujtahid Bin And Kasimo, Elfred Khalimatus Nur Eka Agustanti Kharisul Ihsan Kholis, Akbar Nur Kholis, Muhammad Akbar Nur Laili, Nur Fahma La’ally Maula Lian Pandu Farendra Loviana, Novi Mahardika, Juan Vega Maria Do Carmo Da Costa Freitas Masyhuri, Aga Adi Mebung, Konradus Klala Megasari, Elly Meri, Meri Mika Tri Kumala Swandari, Mika Tri Kumala Mosse1, Yosep Muhammad Nurul Fadel Muslikha, Iza Dwi Muttaqin, Saiful Neni Probosiwi Novyananda Salmasfattah Novyananda Salmasfattah Nur Fahma Laili Nur Fahma Laili Nurul Ismillayli Ornai, Andre Mario Sequeira Alves Rahmasari, Kharisma Rasyadan Taufiq Probojati Renaldi Umbu Ndamung Kale Restyana, Anggi Restyana, Anggy Retnowati, Ana Rochmad Krissanjaya Salmasfattah, Novyananda Sanjaya, Rochmad Kris Shinta Mayasari Shinta Mayasari Sueb Sugeng Santoso Syntia Tanu Juwita Syntia Tanu Juwita Syntia Tanu Juwita Syntia Tanu Juwita Syntia Tanu Juwita Tiyas, Eka Wahyuning Tridoso Sapto A Tsamrotul Ilmi Willy Sandhika Wulansari, Ida Septika Yanti, Novirma Yosep Mosse yunadi, frisca dewi Yuneka Saristiana