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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrikultura Jurnal AGROTEKNOLOGI Jurnal Ilmu Dasar AL KAUNIYAH Techno: Jurnal Penelitian AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Agro AJIE (Asian Journal of Innovation and Entrepreneurship) UNEJ e-Proceeding Jurnal Penelitian Pertanian Terapan Panrita Abdi - Jurnal Pengabdian pada Masyarakat Agriprima : Journal of Applied Agricultural Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA) ANNALES BOGORIENSES Agrin : Jurnal Penelitian Pertanian Warta Pengabdian Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Karinov Jurnal ABDINUS : Jurnal Pengabdian Nusantara Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Journal of Community Development BIOVALENTIA: Biological Research Journal PAPUMA: Journal of Comunnity Services Biosaintifika: Journal of Biology & Biology Education Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Annales Bogorienses Jurnal Bioteknologi & Biosains Indonesia Agricultural Power Journal
Parawita Dewanti
Jember University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Jurnal Agro

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Respon kultur antera tembakau terhadap media bernutrisi dan AgNPs secara in vitro Khozin, Mohammad Nur; Ahnaf, Yusuf Dary; Dewanti, Parawita; Restanto, Didik Pudji; Iryono
Jurnal AGRO Vol. 12 No. 2 (2025)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/j.agro.49119

Abstract

Tanaman tembakau (Nicotiana tabacum L.) merupakan tanaman musiman bernilai ekonomi tinggi, namun produksinya di Indonesia terus mengalami penurunan dalam beberapa tahun terakhir. Penggunaan varietas hibrida dapat menjadi solusi karena memiliki produktivitas dan ketahanan penyakit yang lebih baik, tetapi pembentukannya secara konvensional memerlukan waktu lama. Teknik kultur antera dapat digunakan untuk mempercepat pembentukan galur murni karena menghasilkan tanaman homozigot hanya dalam satu generasi. Penelitian ini bertujuan untuk menganalisis interaksi antara jenis media kultur (MS dan Chu’s N6) dengan penambahan beberapa konsentrasi Nanopartikel silver (AgNPs) yaitu 0,0; 2,5; 5,0; 7,5; dan 10,0 mg L⁻¹ terhadap pertumbuhan antera tembakau secara in vitro. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 10 perlakuan dan 3 ulangan, kemudian data dianalisis menggunakan ANOVA dan diuji lanjut DMRT dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa media MS merupakan media terbaik dalam mempercepat dan memperbanyak induksi swelling, kalus, tunas, dan akar, sedangkan konsentrasi AgNPs terbaik diperoleh pada 2,5 mg L⁻¹ yang mampu meningkatkan respons pertumbuhan serta sedikit mengurangi tingkat kontaminasi media. Hasil penelitian ini menunjukkan bahwa kombinasi media MS dan AgNPs dosis rendah mampu meningkatkan efisiensi regenerasi antera tembakau dan berpotensi digunakan sebagai protokol standar dalam produksi galur murni secara cepat, efisien, dan steril di laboratorium kultur jaringan. ABSTRACT Tobacco (Nicotiana tabacum L.) is a seasonal crop with high economic value, but its production in Indonesia has continued to decline in recent years. The use of hybrid varieties can be a solution because they have better productivity and disease resistance, but their conventional formation requires a long time. Anther culture techniques can be used to accelerate the formation of pure lines because they produce homozygous plants in just one generation. This study aims to determine the interaction between the types of culture media (MS and Chu's N6) with the addition of several concentrations of silver nanoparticles (AgNPs), namely 0, 2.5, 5, 7.5, and 10 mg L⁻¹ on the growth of tobacco anthers in vitro. The study used a Completely Randomized Design (CRD) with 10 treatments and 3 replications, then the data were analyzed using ANOVA and further tested with DMRT at the 95% level. The results showed that MS medium was the best medium in accelerating and increasing the induction of swelling, callus, shoots, and roots, while the best concentration of AgNPs was obtained at 2.5 mg L⁻¹ which was able to increase the growth response and slightly reduce the level of media contamination. The findings of this study indicate that the combination of MS medium and low-dose AgNPs can increase the efficiency of tobacco anther regeneration and has the potential to be used as a standard protocol in the production of pure lines quickly, efficiently, and sterilely in tissue culture laboratories.
Co-Authors . Usmadi Abdul jalil Adinda, Intan Dwi Ahnaf, Yusuf Dary Al Ayyubi, Nabila Nur Aisyah Alfian, Firdha Narulita Alifah Farida Sa’adah Alifatul Aqidah Arya Wiranegara Azmi Saleh Bagus Tripama Bambang Soegiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Banun Kusumawardani Budi Kristanto Budi Kriswanto Cesha Ananda Putri Desi Kartika Sutrisno Dwi Setyati Dwika Nano Hariyanto Erma Sulistyaningsih Fanata, Wahyu Indra Duwi Firdausi, Intan Firdha Narulita Alfian Firdha Narulita Alfian Firdha Narulita Alfian Firdha Narulita Alfian Hakim, Mohammad Sulton Harsanti, Restiani Sih Hidayat Hidayat Hidayat Ika Purnamasari Indraloka, Aldy Bahaduri Intan Dwi Ambarwati Inyana Dwi Agustien Iryono Kacung Hariyono Khofifa, Rendryana Aulia Nur Khozin, Mohammad Nur Laela Endah Rahmadhani Laily Ilham Widuri Laily Ilman Widuri, Laily Ilman Lenny Widjayanthi Magfiroh, Illia Seldon Maisaro, Maisaro Maryam, Safira Arikha milga sari Mohammad Ali Mudhor Mona, Muhammad Dima Say Muhammad Hazmi Munandar, Denna Eriani Nadiya, Nisma Riyadh Nafisah Iqmatullah Ni Made Ayu Gemuh Rasa Astiti Nina Oktaria Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Olandino Tome Francisco Dorosario de Sousa Popy Hartatie Hardjo Pralampita, Pulong Wijang Prayoga, Mohammad Candra Purnama Okviandari Purnama Okviandari, Purnama Puspito, Agung Nugroho Putri, Cesha Ananda Putri, Widya Kristyanti Raden Soedradjad Rahmadhani, Laela Endah Raisah Bani Ratnasari, Tri Restanto, Didik Restanto, Didik Pudji Ristiyana, Suci Rizky Harikurniawan Rudi Joelijanto Santoso, Luthfi Nur Aprillia Sasmita, Heni Dwi Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Siti Kamalia Siti Nurul Afidah Slameto Slameto Soegiharto, Bambang Sri Hartatik Sulistiyono Syafira Fatihatul Husna Taufik, Rahadian Falqi Tri Agus Siswoyo Tri Agus Siswoyo TRI HANDOYO Tri Wahyu Saputra Ubaidillah, Mohammad Ummi Solikhah, Ummi Veronenci Yuliarbi Farlisa Wachju Subchan Wahyu Indra Duwi Fanata Wakhidaturrohmah, Ninik Wardatus Sholeha Widuri, Laily Ilham Widya Kristiyanti Putri Wulan Nursyiam Ningtiyas Wulanjari, Distiana Yani Corvianindya Rahayu