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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Buletin Veteriner Udayana Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS Biotropika BULETIN FISIKA Cassowary Jurnal Sewaka Bhakti Widyanatya: Jurnal Pendidikan Agama dan Seni Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika

Ida Bagus Gede Darmayasa

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 268954

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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THE POTENTIAL OF CHITINASE ENZYMES PRODUCED BY CHITINOLYTIC BACTERIA ISOLATED FROM SHRIMP SHELLS AS BIOCONTROL CANDIDATES IN CONTROLLING PLANT PESTS Faldy Efriant Pangemanan; Ida Bagus Gede Darmayasa; Joko Wiryatno
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Plant pests is one of the problem in agriculture sector that can cause decreasing of harvest productivity. Plant pest invasion will be increased in rainy season, so it need to be controlled. Biological control by chitinolytic bacteria is one of many ways to avoid the using of chemical pesticide. The purpose of this research to find the type of chitinolytic bacteria and utilizing the enzyme chitinase as plant pest biocontrol. Test has been done with spray method of 5 mL with 10%, 20%. 30%, 40% and 50% concentration with 7 treatments include positive and negative controls and 3 repetition. Observation of larva death done for 24 hours after spray, where every 6 hours observations are made. Pseudomonas is a genus that has been identified as chitinolytic bacteria. Larva death is indicated by with percentage result insignificant death which is shown at the 10 – 30% concentration. The percentage result significant death which is shown at the 40 – 50% concentration. 40% and 50% is most effective concentration in killing fruit fly (Drosophila sp.).

DAYA HAMBAT INFUSA RIMPANG KUNYIT (Curcuma longa Linn) TERHADAP PERTUMBUHAN Escherichia coli dan Vibrio sp. pada IKAN KERAPU LUMPUR (Epinephelus tauvina) di PASAR KEDONGANAN KABUPATEN BADUNG, BALI Ni Putu Sinta Puspa Dewi; I. B. Gede Darmayasa; Ni Wayan Sudatri
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACTFish is perishable food because it contains high enough protein and water, therefore to easy damaged by microorganism. Then the control of mircoorganism should be done to maintaining the quality of fish to stay awake.Turmeric is one of the herbs that has compound content curcumin and fenolik being able to control the growth of contaminants microorganism. The objective of this research is to find out the power of immersion of mudfish grouper (Epinephelus tauvina) by infusa turmeric rhizome (Curcuma longaLinn) through sampled from Kedonganan Market, Bali. The treatment given to mudfish grouper was turmeric rhizome infusion withconcentration of 0%, 5%, 10%, 15% and 20% tested in vitro and in vivo on the growth of E. coli and Vibrio sp. The resourcesinhibited turmeric infusa rhizome is determined by counting the population of bacteria test after treatment by the method of dilution sampling (Plating Method). The results showed that turmeric rhizome infusion was able significantly (P<0,05) inhibitionto the growth of E. coli and Vibrio sp. both in vitro and in vivo. The control (0%) in vitro population E. coli and Vibrio sp. each of 5,23x102 CFU/g and 4,98x102 CFU/g higher than with the treatment of concentration 5%, 10%, 15% and 20%. Population E. coli and Vibrio sp. in testing by in vivo (concentration 0%) each is obtained 4,17x102 CFU/g dan 4,20x102 CFU/g in statistic is different (P<0,05) with the concentration 10%, 15% and 20%. Keywords: Epinephelus tauvina, Curcuma longa Linn, E. coli, Vibrio sp.

ISOLASI DAN IDENTIFIKASI BAKTERI PELARUT FOSFAT POTENSIAL PADA TANAH KONVENSIONAL DAN TANAH ORGANIK Ilham .; Ida Bagus Gede Darmayasa; Retno Kawuri
SIMBIOSIS Vol 2 No 1 (2014)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.8 KB)

Phosphate solubilizing bacteria are decomposer bacteria functioning as major role in soilenrichment. That is because it is able to perform phosphate dissolution mechanism by excreting lowparticles weight of organic acids. The bacteria utilize simple carbon compounds (rootexudatesofplantsandthe restof theplant).Phosphate solubilizing bacteria activity will be high at atemperature of 300C - 400C. This study aims to isolate and identify thephosphate solubilizingbacteria intheconventional soil and organic soil originated from Japan. Conventional and organic soil sample aresent from Collage Of Agricultural Ibaraki University in Japan. The Isolation of pathogenic bacteria isperformed by the dilution method (platting method). Results of isolation of bacteria lisolates obtained 2on ground phosphate conventional solubilizing (TKO1 andTKO2) and 4 isolates of bacteria onsoilorganicsolubilizingphosphate(TOR1, TOR2, TOR3andTOR4). Bacteria lisolates TKO2 highestphosphate solubilizing. Based on the identificati on results using the Microgen ™ Kit GNA + B - IDSystem and Determine Bergeys 's Manual of Biology (Garrity et al., 2006)TKO2 isolate is identified asYersinia sp.Keywords : Phosphate solubilizing bacteria, conventional soil, organic soil, Yersinia sp.

EFFECTIVENESS OF Trichoderma asperellum TKD WITH MEDIATOR OF MANURE TO CONTROL FUSARIUM DISEASE ON RED PEPPER PLANT (Capsicum annum L.) Nyoman Mega Antari; Ida Bagus Gede Darmayasa; Junita Hardini
SIMBIOSIS Vol 8 No 2 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Red pepper is agricultural products in Indonesia which is often attacked by fusarium wilt disease due to the fungus Fusarium oxysporum f.sp. capsici, so in this study aims to control of fusarium wilt disease using Trichoderma asperellum TKD with mediator of manure. Method in this study is RAL (Completely Randomized Design) with 7 treatments P1 = Control I (without treatment); P2 = Control II (F.oxysporum f.sp. capsici (disease)); P3 = disease + T. asperellum TKD 10 mL; P4 = disease + T. asperellum TKD 20 mL; P5 = disease + T.asperellum TKD 30 mL; P6 = disease + T. asperellum TKD 40 mL; P7 = disease + T. asperellum TKD 50 mL. The results showed that T.asperellum TKD was able to inhibit F.oxysporum f.sp. capsici in in vitro by 100%. The highest percentage of fusarium wilt disease (100%) is shown in plants that are only given F.oxysporum f.sp. capsici and without T. asperellum TKD, as well as plant height, total of fresh and dried leaves in plants added by T. asperellum TKD is better compared to plants that are not given T. asperellum TKD. The best plant height, number of freh leaves, and lowest number of dry leaves found in plants with 50 mL of T.asperellum TKD. Keywords: biocontrol, pathogenic fungi, Trichoderma asperellum TKD

ISOLASI, IDENTIFIKASI DAN UJI POTENSI BAKTERI YANG BERPERAN PADA PENGOLAHAN AIR LIMBAH YANG MENGANDUNG RHODAMIN B DALAM BIOSISTEM TANAMAN Sang Ayu Sri Satya Laksmi Utari; Ida Bagus Gede Darmayasa; I Wayan Budiarsa Suyasa
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.366 KB)

Rhodamin B is a dangerous synthetic dyes substances used as a fabric dye in thetextile industry. Biodegradation is one way that is used in the processing of liquid wasteRhodamin B. Biodegradation technique is leveraging the ability of microbial activitiesdegrade or eliminate pollutant compounds. The main objective of this research was toinvestigate the characteristic of bacteria that are capable of living on wastewater containingRhodamin B and the effectiveness of single and microbial consortium isolates to degradeRhodamin B on wastewater. The isolation of the bacteria was done by plating method and theidentification of the bacteria by using a Kit API 20E. Test of bacterial potential of RhodaminB dyes substance carried out in the Microbiology Laboratory, Biology Department, Facultyof Science, Udayana University. It was found in this research that five bacterial isolates wereobtained (Pseudomonas sp., Shigella spp., Stenotrophomonas sp., Pasteurella sp. dan Proteussp.). Pasteurella sp. had the highest percentage degrade effectiveness of 40.55%. Microbialpotential degrade Rhodamin B by Pasteurella sp. dan Proteus sp. results showed significantdifferences (P <0.05) to control.Keyword: bacteria, biodegradation, Rhodamin B

OBSERVATION OF TYPES OF GULMA IN CORN (Zea mays L.) PLANT PLANTS IN LAND SAWAH DESA BONGKASA ABIANSEMAL BADUNG, BALI PROVINCE Komang Krisna; Martin Joni; I. B.Gd. Darmayasa
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Research on weed inventory in maize land aims to see the diversity of existing weeds growing on the area of ??corn (Zea mays (L.)) in the fields of Bongkasa Abiansemal Badung Village, Bali Province. This observation will be expected to obtain information about the types of weeds that exist, so that weeds and control of weeds in corn (Zea mays (L.)) are optimally implemented. The study was conducted from June to August 2018. In observations using an exsplorating sampling method. In order to produce better and more systematic results, observations were made to make lanes and observations on the research land so that it could easily observe the growth of disturbing plants growing on the observed maize crops. In a 1-month old corn plant, 8 tribes were found with 16 species. In the 2month old corn plant, 14 tribes were found with 28 species of weeds, while in the 3-month-old maize there were 22 tribes with 40 species of weeds. The 22 tribes are Amaranthaceae, Asteraceae, Balsaminaceae, Capparidaceae, Commelinaceae, Convolvulaceae, Cyperaceae, Euphorbiaceae, Goodeniaceae, Laminaceae, Mimosaceae, Loganiaceae, Malvaceae, Onagraceae, Oxalidaceae, Poaceae, Portulacaceae, Rubiaceae, Scrophulariaceae, Solanaceae, Urtcaceae, Violaceae, Urtcaceae, with 40 species namely Amaranthus gracilis (Desf), Amaranthus spinosus (L), Ageratum conoyzoides L., Eclipta alba L., Emilia sonchifolia (L.), Vernonia cinerea (L, Synedrella nodiflora (L.), Tridax procumbens, Impatatiens platypetalaL Cleome rutidosperma DC, Cleome viscosaL, Murdania nudiflora (L.) Brenan, Impomea triloba L., Paedenia foetida L., Impomea gassicaulis Rot, Cyperus rotundus L. Kylliangia monocephala (Endl). Euphorbia hirta L. Phyllantus urinaria L., Phyllantus debilis Klein. Elephantopus scaber C., Basilicum polystachyon (L.), Mimosa pudica (L). Spigelia anthelmia L., Sida rhombifolia L., Ludwigia perennis L., Oxalis corniculata L. Dactyloctenium aegy ptium (L.), Digitaria ciliaris (Retz.), Eragrotis tenella (L.), Echinoclhoa colonum (L.), Elusine indica L., Portulaca oleraceae L., Hedyotis diffusa (L.) Lamk, Lindernia crustacea (L.), Scoparia dulcis L., Physalis angulata L., Capsicum frutescens, Urtica grandidentata L, Hybanthus attenuates L. Keywords: weed, Zea mays L., Bongkasa Village.

UJI FITOKIMIA DAN DAYA HAMBAT EKSTRAK DAUN JUWET (Syzygium cumini) TERHADAP PERTUMBUHAN Escherichia coli DAN Staphylococcus aureus ATCC Kadek Sudarmi; Ida Bagus Gede Darmayasa; I Ketut Muksin
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

Bacterial Escherichia coli dan Staphylococcus aureus ATCCis a normal flora bacteria that naturally exist human body. This bacteria may by pathogenic if it exceeds acertain limits. To prevent the disease caused, need to be overcome by using herbal ingredients one of them is juwet leaves (Syzygiumcumini).The purpose of the study was to determine wheter the extract of juwet leaf (S. cumini) can inhibited the growth of E. coli and S. aureus ATCC and to know compounds contained in juwet leaf extract (S. cumini). This research used diffusion wells methodwith 6 treatments and 4 replications. Date analysis using completely randomized design (CRD) with using ANOVA variance analysis. If the date obtained has a real difference test level at the 5% (P? 0,5) that continue with Duncan test. Concentration of the extract tested was 0%, 5%, 10%, 15%, 25% and 50%. Juwet leaf extract is able to inhibited the growth of E. coli and S. aureus ATCC which is shown by the formation of clear zone. Concentration extract leaf which is effective to inhibit E. coli and S. aureus ATCC is the concentration 50% (18,9 mm) for E. coli and (16,5 mm) for S. aureus ATCC. Screening of phytochemical test of juwet leaf extract positive containing alkaloids, phenolic, steroids and saponins. Keywords : juwet leaf extract (Syzygium cumini), Escherichia coli, Staphylococcus aureus ATCC

BIODEGRADATION OF CACAO (Theobroma cacao L.) RIND WASTE BY Aspergillus niger WITH VARIATION OF INOCULUM CONCENTRATION AND INCUBATION TIME Alfian Siswanto; Ida Bagus Gede Darmayasa; I Ketut Muksin
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Crop waste or cacao (Theobroma cacao L.) processing waste that doesn’t handle with care can polluting the environment around the farm. Rind of cacao can be used for composting, but it can be less effective because the nutrient of fresh cacao rind too complex and the ratio of C/N too high. This research intention to knows the potential of Aspergillus niger to degrade cellulose on waste cacao rind who have high ratio of C/N, with combination inoculum concentration and incubation time. The methods of this research begin with isolation and identification of A. niger from litter of cacao field, then the in vitro test done by CMCA (Carboxyl Methyl Cellulose Agar) selective media, and then the cacao rind was testing by in vivo method. The results of this research shown that A. niger can degrade cellulose by in vitro ways, it can be seen by the clear zone that formed on CMCA selective media. The in vivo test on cacao rind shown decreasing of C/N ratio between 18,940%-40,623%. Combination of A3B3 treatment (15 mL inoculum concentration, 28 days incubation time) indicate the lowest C/N ratio 18,940%.

ISOLASI DAN OPTIMALISASI PERTUMBUHAN BAKTERI PELARUT FOSFAT (BPF) PADA LIMBAH TAHU CAIR DENGAN MENGGUNAKAN KONSENTRASI KARBON (C) YANG BERBEDA Siti Didah Alawiah; Ida Bagus Gede Darmayasa; I Ketut Sundra
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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This research aimed to determine the concentration giving Carbon (C) the right to know the waste liquid as solvent Bacterial growth media Phosphate (BPF) performed in the Laboratory of Microbiology, University of Udayana. Experimental research method by adding Dextrosa concentrations varying the liquid waste that has been given out bacterial isolates phosphate solvent the concentration of 0 g (as a control), 0,54 g, 0,77 g and 1,00 g Dextrosa. The results showed the growth of bacteria Solvent Phosphate (BPF) E. gergoviae no significant differences (P <0.05) between the control and treatment of 0,54 g and 1,00 g, 0,77 g where as treatment showed significant differences (P> 0.05) are marked with the number of total number of bacterial colonies of E. gergoviae as many as 2,45 CFU / mL. Keywords : Tofu Liquid Waste, Bacteria Solvent Phosphate (BPF) E. gergoviae, Dextrosa.

DAYA HAMBAT EKSTRAK KASAR DAUN KALIANDRA (Calliandra calothyrsus Meissn.) TERHADAP JAMUR KONTAMINAN PADA PAKAN KONSENTRAT AYAM RAS PEDAGING I Gede Sugita Artana; IB.G Darmayasa; Meitini W. Proborini
SIMBIOSIS Vol 4 No 2 (2016)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (733.435 KB)

ABSTRACT This aims of the study were to determine the species of fungi contaminated chicken broiler consentrate and to find out the inhibition of crude extract's leaves of caliandra. The samples were taken in Selat, Karangasem. Maceration method was conducted to extracted leaves of caliandra, disk diffusion agar method was conducted to find out the inhibition of crude extract leaves of caliandra. Result showed that there were six types of fungi on chichken broiler consentrate such as Aspergilus flavus, Aspergilus niger, Aspergillus fumigatus, Penicillium brevicompactum, Penicillium candidum dan Trichoderma viridae. The result also showed that 100.000 ppm extract concentrate inhibit Aspergillus flavus growth on 12.72 ± 0,27 mm. Keywords : fungi, inhibition strength, crude extract’s, caliandra, Aspergillus flavus

Co-Authors A.A.S. A. Sukmaningsih Alayna Himawan Alfian Siswanto Anak Agung Gede Indraningrat Ariyanti Ni Kadek Ayu Amelia Bekti Revi Rahayu Cokorda Putra Deny Christine Sidabutar Dwi Dirgantini, Anak Agung Faldy Efriant Pangemanan Fania Agustini Flavia Fionessa Falenda Shanzet I Gede Sugita Artana I Gusti Agung Ayu Ratnawati I Gusti Ayu Laras Pratama Putri I Ketut Gede Rudita I KETUT MUKSIN I Ketut Sundra I Komang Adi Widyastama I Luh Putu Wiwin Astari I Made Dira Swantara I Made Sara Wijana I Made Sugiarta I Nyoman Winyana, I Nyoman I Putu Agus Hendra Wibawa I W BUDIARSA SUYASA I Wayan Kasa I Wayan Sudiarsa I Wayan Sukadana I.P.G. Ardhana Ida Ayu Gde Yadnyawati Ida Bagus Made Suaskara Ilham . Inna Narayani Joko Wiryatno Junita Hardini Kadek Feby Widyantari Kadek Sudarmi Komang Kartika Indi Swari Komang Krisna Lalu Muhammad Sakti Surya Jagat Lintang Pradhanawati Made Ria Defiani MADE SUSUN PARWANAYONI Martin Joni Meitini Proborini Wahyuni Nadia Khairunnisa Nasha Hadi Rahmadanty NI KADEK WIWIK SINTA DEWI Ni Komang Ayu Kumala Dewi Ni Komang Deny Julyeda Ni LUH ARPIWI Ni Luh Putu Eka Suartini Ni Made Indah Suandewi Ni Made Susun Parwanayoni Ni Putu Adriani Astiti Ni Putu Indiani Sahaning Karna Ni Putu Sinta Puspa Dewi Ni Putu Yuliatiningsih Ni Wayan Purni wirathi Ni Wayan Sudatri Nyoman Mega Antari Pande Gde Eka Mardiana Pande Gde Sasmita Julyantoro Pande Putu Christine Putri Purnami Pande Putu Purwaningsih Prayitna Dewi, Ida Ayu Putu Cindy Arista Putu Eka Sudaryatma Ratu Ayu Ningrat Retno Kawuri Rochmalia Juniarti Putri Sang Ayu Sri Satya Laksmi Utari SANG KETUT SUDIRGA Siti Didah Alawiah Siti Nurlatifah SRI LESTARI Suasthi, I Gusti Ayu Suta Arta Yan Ramona Yulita Salma Lani

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