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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Buletin Veteriner Udayana Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS BULETIN FISIKA Cassowary Jurnal Sewaka Bhakti Widyanatya: Jurnal Pendidikan Agama dan Seni Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika
Ida Bagus Gede Darmayasa
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : SIMBIOSIS

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KUALITAS SATE LILIT YANG BEREDAR DI PASAR TRADISIONAL KARANGASEM, BALI DITINJAU DARI Staphylococcus aureus I Gusti Ayu Laras Pratama Putri; Ida Bagus Gede Darmayasa; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.207 KB)

Abstract

This research aims to find out the quality of curled satay in terms of Staphylococcus aureusthat are spread around in the traditional markets of Karangasem, Bali. Samples of the curled satay were taken from the 4 regions in Karangasem (Selat, Rendang, Sidemen, and Seraya), every region takes 1 traditional market and every market are owned by 2 salesman. The method used in this study is the method of Platting Method (Pelczar and Chan, 2005).  The results shows that the highest of curled satays in traditional markets of Karangasem is found in S. aureusbacteria was obtained for up to 73 x 102 CFU/g found in Sinduwati market, Sidemen on salesman 1 and 57 x 102 in Seraya Tengah market, on salesman 1. Based on the tests that has been done shows that a number of curled satay sold in traditional markets of Karangasem, Bali has exceeded the threshold amount Indonesian National Standard. The maximum lilit of microbial impurities in satay ready consumption for S. aureusbased on Indonesian National Standard is 102CFU/g. Keywords :Curled satay,Platting Method, Staphylococcus aureus, traditional market
DAYA HAMBAT EKSTRAK LENGKUAS PUTIH (Alpinia galanga L.) TERHADAP PERTUMBUHAN Escherichia coli O157:H7 dan Staphylococcus epidermidis Deny Christine Sidabutar; Ida Bagus Gede Darmayasa; Junita Hardini
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.446 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p10

Abstract

Escherichia coli O157:H7 and Staphylocccus epidermidis are bacteria that can cause disease in humans. Alternative control efforts are very important because the use of antibiotics can cause adverse side effects. This study aims to obtain extracts of white galangal (Alpinia galanga L.) in inhibiting the growth of E. coli O157:H7 and S. epidermidis. In this test, white galangal extract was macerated with three solvents at different polarity levels (96% ethanol, n-hexane and ethyl acetate). The ability of white galangal extract in inhibiting bacteria was tested using the Kirby Bauer method (paper disc). The concentration of galangal extract tested was 0%; 5% ; 10% ; 15%; 20% (w/v), control (ciprofloxacin) and MIC (Minimum Inhibition Concentration). To determine the toxicity of the extract, the LC50 test was carried out. Meanwhile, to determine the class of compounds contained in the galangal extract, phytochemical tests were carried out using the phytochemical screening method. The results showed that white galangal extract with 96% ethanol as solvent and n-hexane was able to inhibit the test bacteria. Inhibition against E. coli O157:H7 was most effective in n-hexane solvent with an extract concentration of 20% (inhibition zone diameter 20.2 mm). while for S.epidermidis was n-hexane solvent with a concentration of 20% (inhibition zone diameter 23.5 mm). LC50 against E. coli O157:H7 with 96% ethanol and n-hexane as solvents 11.2% and 10.4%, respectively. Meanwhile, the LC50 of S. epidermidis with 96% ethanol and n-hexane solvents were 9.9% and 9.3%, respectively. Qualitatively, white galangal plant extract contains a class of flavonoid compounds, saponins, terpenoids, and tannins.
POTENSI EKSTRAK BAWANG PUTIH (Allium sativum L.) DALAM MENGHAMBAT PERTUMBUHAN JAMUR Colletotrichum acutatum PENYEBAB PENYAKIT BUSUK PADA BUAH CABAI MERAH (Capsicum annuum L.) PASCA PANEN Fania Agustini; Ida Bagus Gede Darmayasa; Ni Luh Arpiwi
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.518 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p08

Abstract

This study aims to find out the potency of garlic extract (Allium sativum L.) to the growth of Colletotrichum acutatum fungus which cause rot of red chili fruit post-harvest. The treatments were the administration of garlic extract in-vitro and in-vivo. In the in-vitro test, the inhibition of garlic extract was measured using well diffusion method. The in-vivo test was conducted by measuring the diameter area of C. acutatum on red chilies after treatments. The concentration of garlic extract given was 15%(b/v), 20%(b/v), 25%(b/v), 30%(b/v), and 35%(b/v) with positive control of synthetic fungicides and negative control of ethanol solvents 95% and 4 times replications. The average diameter result on the 25%(b/v) concentration of bland power test had the most effective bland zone diameter of 20.1±0.08. Statistically different was real (P?0.05) with negative controls. While in vivo tests the average area of infection area at a concentration of 35%(b/v) had an average diameter area of infection area which was best in inhibiting the growth of C. acutatum fungus.
POTENCY OF Trichoderma asperellum TKD FILTRATE IN INHIBITING Aspergillus parasiticus CONTAMINATION IN PEANUT SEEDS (Arachis hypogaea L.) Nasha Hadi Rahmadanty; Ida Bagus Gede Darmayasa; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p02

Abstract

iThis researchhaimed to determineethe potency off Trichoderma asperellum TKD ffiltrate in inhibiting Aspergillus parasiticus contamination in peanut seeds. The treatment given to A. parasiticus was done in vitro and in vivo. In vitro there were three types methods, dual culture and the inhibition test of the T.asperellum TKD ifiltrate using the diffusion well and the colony method with concentrations of 40% (v/v), 50%(v/v), 60%(v/v),70%(v/v) and 80%(v/v). While in vivo, it was done on peanut seeds added T.asperellumiTKD filtrate with some concentrations similar to the treatment given in vitro. The parameters measured in vitro test were determined through PIRG (Percentage Inhibition of Radial Growth) and diameter inhibition zone, in vivo test parameter was the A. parasiticus colony populations. The results showed T.asperellum TKD filtrate significantly (P?0,05) was able to inhibit A. parasiticus growth iin vitro and iin vivo. The percentageeof T.asperellum TKD bland power against A.parasiticus in vitro with the highest dual culture method was 60.65%. In the method of colony and diffusion well, the concentration of 80% was the highest concentration in inhibiting A. parasiticus, which were 83.00±1.60% and 15.09±0.12 mm. T.asperellum TKD filtrate with a concentration of 80% added to peanut seeds had the highest inhibition on the number of population of A. parasiticus colonies with a percentage decrease of 88.3%.
Co-Authors A.A.S. A. Sukmaningsih Alayna Himawan Alfian Siswanto Ariyanti Ni Kadek Ayu Amelia Bekti Revi Rahayu Cokorda Putra Deny Christine Sidabutar Dwi Dirgantini, Anak Agung Faldy Efriant Pangemanan Fania Agustini Flavia Fionessa Falenda Shanzet I Gede Sugita Artana I Gusti Agung Ayu Ratnawati I Gusti Ayu Laras Pratama Putri I Ketut Gede Rudita I KETUT MUKSIN I Ketut Sundra I Komang Adi Widyastama I Luh Putu Wiwin Astari I Made Dira Swantara I Made Sara Wijana I Made Sugiarta I Nyoman Winyana, I Nyoman I Putu Agus Hendra Wibawa I W BUDIARSA SUYASA I Wayan Kasa I Wayan Sudiarsa I Wayan Sukadana I.P.G. Ardhana Ida Ayu Gde Yadnyawati Ida Bagus Made Suaskara Ilham . Inna Narayani Joko Wiryatno Junita Hardini Kadek Feby Widyantari Kadek Sudarmi Komang Kartika Indi Swari Komang Krisna Lalu Muhammad Sakti Surya Jagat Lintang Pradhanawati Made Ria Defiani MADE SUSUN PARWANAYONI Martin Joni Meitini Proborini Wahyuni Nadia Khairunnisa Nasha Hadi Rahmadanty NI KADEK WIWIK SINTA DEWI Ni Komang Ayu Kumala Dewi Ni Komang Deny Julyeda Ni LUH ARPIWI Ni Luh Putu Eka Suartini Ni Made Indah Suandewi Ni Made Susun Parwanayoni Ni Putu Adriani Astiti Ni Putu Indiani Sahaning Karna Ni Putu Sinta Puspa Dewi Ni Putu Yuliatiningsih Ni Wayan Purni wirathi Ni Wayan Sudatri Nyoman Mega Antari Pande Gde Eka Mardiana Pande Gde Sasmita Julyantoro Pande Putu Purwaningsih Prayitna Dewi, Ida Ayu Putu Cindy Arista Putu Eka Sudaryatma Ratu Ayu Ningrat Retno Kawuri Rochmalia Juniarti Putri Sang Ayu Sri Satya Laksmi Utari SANG KETUT SUDIRGA Siti Didah Alawiah Siti Nurlatifah SRI LESTARI Suasthi, I Gusti Ayu Suta Arta Yan Ramona Yulita Salma Lani