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All Journal HAYATI Journal of Biosciences Indonesian Journal of Tropical and Infectious Disease Sari Pediatri Jesya (Jurnal Ekonomi dan Ekonomi Syariah) Oceana Biomedicina Journal Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Indian Journal of Forensic Medicine & Toxicology Griya Journal of Mathematics Education and Application Narra J Folia Medica Indonesiana
Usman Hadi
Dept. Of Internal Medicine School Of Medicine Airlangga University/Dr. Soetomo Hospital
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health

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The role of mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) on cytokine production during dengue virus infection Masyeni, Sri; Kuntaman, Kuntaman; Aryati , Aryati; Sofro, Muchlis AU.; Hadi, Usman; Mastutik, Gondo; Purnomo, Windu; Santosa, Agus; Iqhrammullah, Muhammad; Yohan, Benediktus; Nelwan, Erni J.; Sasmono, R. Tedjo
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.167

Abstract

Inability to understand the pathogenesis of severe dengue, in particular the control mechanism of immune responses, has led to high mortality rate for patients with dengue shock syndrome (DSS). The aim of this study was to determine the control mechanism of cytokine production by mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) during DENV infection. Peripheral blood mononuclear blood cells (PBMC), isolated from healthy individuals, were infected with dengue virus (DENV)-2 strain SJN-006 Cosmopolitan genotype (isolated from Bali, Indonesia). The relative gene expression of SOCS-3, TLR-3, NFκB, and the cytokine genes (interleukin (IL)-6, IL-8, interferon inducible protein 10 (IP-10), and macrophage inflammatory protein-1 beta (MIP-1β)) were measured using qRT-PCR at 6, 12 and 24 hours post infection (hpi). Student t-test and Mann-Whitney test were used to compare the gene expressions while causal correlations were analyzed using regression test and path analyses. DENV-2 infection increased the gene expression of SOCS-3, TLR-3, and NFκB after 12 and 24 hpi. The expression of IL-6, IL-8, IP-10, and MIP-1β genes was increased and peaked at different times post-infection. NFκB and SOCS-3 genes likely have role in the upregulation of IL-8 and IL-6 gene expression, respectively. MIP-1β gene expression was significantly induced by both NFκB and SOCS-3. In conclusion, our study suggested that SOCS-3, TLR-3, and NFκB are important in regulating the production of IL-6, IL-8, IP-10, MIP-1β during early phase of DENV-2 infection. This enriches our understanding on pathogenesis pathway of DENV-associated cytokine storm.
Effector function and neutrophil cell death in the severity of sepsis with diabetes mellitus Margalin, Brilliant; Arfijanto, Muhammad V.; Hadi, Usman
Narra J Vol. 4 No. 1 (2024): April 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i1.532

Abstract

Sepsis, a life-threatening condition resulting from immune dysregulation, is typically triggered by bacterial infections and commonly coexists with diabetes mellitus. Neutrophils are the first responders to infection and require regulated activation to control pathogen and damage-associated molecular patterns. Dysregulation of neutrophil activation leads to uncontrolled inflammatory responses, often observed in both sepsis and diabetes patients. Neutrophil dysregulation, characterized by effector dysfunction and inadequate cell death processes, can serve as a biomarker for assessing sepsis severity, particularly in diabetic patients. This review article provides information on the relationship between effector function, neutrophil cell death, and the severity of sepsis in individuals with diabetes mellitus, aiming to shed light on the mechanisms underlying sepsis progression. Topics covered in the review include an overview of effector function of neutrophil cells, mechanisms of neutrophil cell death, and dysregulation of effectors and neutrophil cell death processes in sepsis severity with diabetes mellitus.
Association Between Neopterin Levels and Outcome in 30 Day HIV/AIDS Naive Patients Juanita, Lydia; Hadi, Usman; Arfijanto, Vitanata
Biomolecular and Health Science Journal Vol. 2 No. 2 (2019): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.175 KB) | DOI: 10.20473/bhsj.v2i2.14965

Abstract

Introduction: HIV/AIDS is still a major problem throughout the world even in Indonesia. Some markers can provide information regarding the description of immune activity in HIV/AIDS. Neopterin produced by macrophages as a catabolic result of Guanosine Triphosphate (GTP) is one of them. Neopterin has been widely studied as a prognostic indicator of the course in HIV/AIDS. Until now there have been no studies examine the association between neopterin levels and outcomes in 30 days of HIV/AIDS patients.Methods: A prospective longitudinal analytic study involved 56 samples of HIV/AIDS naive patients from January to April 2019. Serum neopterin levels were measured by the Enzyme Linked Immunosorbent Assay (ELISA) method. Outcome is a living condition or death within the first 30 days of the patient being treated. Discriminant function analysis was used to determine the association between variable and controlling confounding factors using the "R" program. Outputs of p value was significant if <0.05.Results: The average age of the study subjects was 39.7 ± 11 years old with predominantly male gender (71.4%). The median serum neopterin level of the study subjects was 168.13 nmol/L with the lowest levels of 11.78 nmol/L and the highest level of 196.95 nmol/L. Outcomes were grouped live and died, each group at 50%. There was a significant positive association between serum neopterin levels and outcomes in 30 days (p=0.02)Conclusion: Role of neopterin levels still need to be proven as a prognostic factor by evaluating other factors that influence the outcome of HIV/AIDS patients.
Pengaruh Model Pembelajaran Kooperatif Tipe TGT berbantuan Quizizz Terhadap Kemampuan Literasi Matematis Siswa Hadi, Usman; Hayati, Laila; Novitasari, Dwi; Sripatmi, Sripatmi
Griya Journal of Mathematics Education and Application Vol. 5 No. 2 (2025): Juni 2025
Publisher : Pendidikan Matematika FKIP Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/griya.v5i2.615

Abstract

This study aims to determine the effect of the cooperative learning model, specifically the Teams Games Tournaments (TGT) type assisted by Quizizz, on the mathematical literacy skills of 11th-grade students at MAN 1 Mataram in the academic year 2024/2025. This research is a quasi-experimental study using a posttest-only control group design. The population consisted of three 11th-grade science classes with a total of 118 students, and the sample included two classes: XI IPA 1 as the control group, which received direct instruction, and XI IPA 2 as the experimental group, which received the TGT model assisted by Quizizz. The sampling technique used was cluster random sampling. Data were collected through a mathematical literacy skills test administered after the intervention and were analyzed using normality and homogeneity tests. Based on the results, a non-parametric test chi-square test was employed along with the calculation of the effect size. The findings showed a difference in the average scores between the control group (49) and the experimental group (78), indicating the influence of the TGT model assisted by Quizizz on students' mathematical literacy. The effect size value of d = 0.465 falls within the medium category, suggesting a moderate impact. Therefore, the TGT cooperative learning model assisted by Quizizz contributes positively to enhancing students' mathematical literacy, particularly in function-related topics such as trigonometry, logarithms, and algebra. Future studies are recommended to further explore the implementation of this model across different educational levels, subject matter, and broader learning contexts.
Quantity of Antibiotic Use and Resistance Pattern of Gut Normal Flora Escherichia coli at Intensive Care Unit and Tropic Infection Ward, Dr Soetomo Hospital, Surabaya, Indonesia Sholeh, Mohammad Akbaruddin; Kuntaman, Kuntaman; Hadi, Usman
Folia Medica Indonesiana Vol. 56, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Resistance to antibiotics is increasing worldwide, including in Indonesia. The use of antibiotics is the most common cause of microorganism resistance. Individuals who received antibiotic therapy impacton changes of normal micro-flora resistance through selective pressure. This study aims to analyze the relationship of the quantity of antibiotic use with the pattern of resistance of gut normal flora Escherichia coliamong patients in Intensive Care Unit (ICU) and Tropic Infection Ward, Dr. Soetomo Hospital, Surabaya. This study was cross-sectional design with 64 samples (32 the ICU and 32 in Tropic Infection Ward). The total sample were collected for three Months. Identification of intestinal microflora was done with McConkey differential selective medium, followed by IMViC biochemical test, and sensitivity test by antibiotic disc diffusion method. Data were analyzed with Chi square test and Fisher'sExact test. There was no significant difference (p=0.441) in the quantity of antibiotic use between ICU and Tropic Infection Ward. There was no significant difference (p>0.05) in normal intestinal flora Escherichia coli resistance pattern between Tropic Infection Ward and ICU against12 types of antibiotics. Ceftriaxone was the most widely used antibiotic in the Tropic Infection Ward of 54 DDD and ICU of 100 DDD. The highest percentage of intestinal flora Escherichia coli resistance at the ICU was ceftriaxone as manyas 18 (56.3%) and at Tropic Infection Ward on ciprofloxacin and levofloxacin 20 (62.5%). The other study in Primary Health center, showed that theresistance rates of both wards were significantly different as compared to intestinal flora of patients in primary health center (p <0.001), in whichthe use of antibiotics in primary health center was also lower (1.6 DDD). There was no significant difference (p>0.05) The pattern of bacterial resistance between patient with and without antibiotic use at Tropic Infection Ward and ICU Conclusion: the quantity of antibiotic use was not significantly different against resistnt gut flora between patients in ICU and Tropic Infection ward. The pattern of bacterial resistance between patient with and without antibiotic use was also not significantly different.
Molecular Genotyping of Tsst-1 Gene Staphylococcus Aureus from Clinical Specimen Puspitarini, Marinda Dwi; Setyarini, Wahyu; Hadi, Usman; Kuntaman, Kuntaman
Folia Medica Indonesiana Vol. 57, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Staphylococcus aureus (S. aureus) is the most common pathogen in humans that causes various infections. Variations in infections due to S. aureus are related to the presence of virulence factors, one of which is Toxic Shock Syndrome Toxin-1 (TSST-1) which is the main cause of Toxic Shock Syndrome (TSS). This study aimed to explore the genetic pattern of the tsst-1 genes among MSSA and MRSA from clinical isolates of patients at Dr. Soetomo Academic Hospital, Surabaya-Indonesia. PCR examination was performed on 106 clinical samples of S. aureus isolates to determine the presence of the tsst-1 genes; subsequently, phylogenetic analysis was performed. The positive tsst-1 genes were found in 3 (5.7%) MSSA isolates and 2 (3.8%) MRSA isolates from 106 samples, of which were divided into 53 MSSA isolates and 53 MRSA isolates. No significant difference was noticed between the tsst-1 genes on both the MSSA and MRSA bacteria (p = 0.648). The positive tsst-1 genes were found in 1 (4.5%) of pus specimens and 2 (16.7%) of blood specimens from MSSA isolates. However, the positive tsst-1 genes were found in neither sputum nor urine specimens. The genes were found in 2 (16.7%) of blood specimens from MRSA isolates. Based on the phylogenetic tree, Indonesian tsst-1 isolates belonged to the same clade as Japan, Iran, Iraq, India, Egypt and the United Kingdom isolates. The prevalence of tsst-1 genes of both MSSA and MRSA from clinical isolates in Dr. Soetomo Academic Hospital Surabaya are 5.7% and 3.8%, respectively. The tsst-1 genes of Surabaya-Indonesia, Japan, Iran, Iraq, India, Egypt and the United Kingdom belonged to the same clade.
Correlation of Population and Environmental Behavior with Rat Density Rate in Plague Disease-Focus, Threatened and Safe Areas in Tutur District, Pasuruan Regency, 2016 Lestari, Evi Noerista; Andajani, Susilowati; Hadi, Usman
Folia Medica Indonesiana Vol. 55, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Plague disease remains a public health problem in the world. According to the International Health Regulations (IHR), an outbreak is an infectious disease that has the potential to cause an outbreak. The purpose of this study was to analyze a significant relationship between human behavior and the environment with the density of the number of rats in the focus, threatened and outbreak-safe areas in Tutur Regency, Pasuruan Regency, in 2016. This was an observational analytic study with cross correlation sectional where the research variables are population behavior, environment, and the number of rat densities observed once at a time. The results showed that there was no significant relationship between the behavior (knowledge, attitude, and practice) of the population with the density of rats in the focus area of the Surorowo hamlet outbreak, in the endangered outbreaks of the Ngaro hamlet, and in the outbreak-safe area of the North Ngandong hamlet in Tutur District. Whereas, there is a significant relationship between environmental conditions and the amount of rat density in the focus area of the outbreak of the Surorowo hamlet (p: 0.047 or p
Analysis of Quality of Antibiotic Usage on Patient with Internal and Surgical Service Ma'rifah, Nurul; Hasmono, Didik; Hadi, Usman; Kuntaman, Kuntaman
Folia Medica Indonesiana Vol. 57, No. 1
Publisher : Folia Medica Indonesiana

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Abstract

It is well-known that hospitals are health facility with the widely use of antibiotics. It is about 13-37% from the total hospitalized patients in developed countries use antibiotic, even in developing countries can reach 30-80%. There is identified correlation between antibiotic use and the development of bacterial resistance. Even though the resistance cannot be eliminated, but its development can be suppressed by the increasing of prudent use of antibiotics. The aim of this study was to determine the quality of antibiotic use on internal medicine and surgical patients in Aisiyah Hospital Bojonegoro. The study was a prospective cross sectional observational analytical study of among patients of internal and surgical who received antibiotic therapy in the period of August - September 2017. The total 50 samples were collected in this study which consists of 33 internal medicine and 17 surgical patients. From 50 samples, there were 16 types of antibiotics with the total use of 81 of antibiotic use. As the result, in internal medicine patients there were 22 (40%) of appropriate use of antibiotics, 4 (7.27%) of inappropriate use and 29 (52.73%) use of antibiotics without indication. In surgical patients, there were 12 (46.15%) of appropriate use of antibiotics, 2 (7.69%) of inappropriate use and 12 (46.15%) use of antibiotics without indication. This study showed that more than 50% of antibiotic use were inappropriate, and mainly antibiotic with no indication, among patients hospitalized in Aisyiyah Hospital Bojonegoro.
The role of mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) on cytokine production during dengue virus infection Sri Masyeni; Kuntaman Kuntaman; Aryati Aryati; Muchlis AU. Sofro; Usman Hadi; Gondo Mastutik; Windu Purnomo; Agus Santosa; Muhammad Iqhrammullah; Benediktus Yohan; Erni J. Nelwan; R. Tedjo Sasmono
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.167

Abstract

Inability to understand the pathogenesis of severe dengue, in particular the control mechanism of immune responses, has led to high mortality rate for patients with dengue shock syndrome (DSS). The aim of this study was to determine the control mechanism of cytokine production by mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) during DENV infection. Peripheral blood mononuclear blood cells (PBMC), isolated from healthy individuals, were infected with dengue virus (DENV)-2 strain SJN-006 Cosmopolitan genotype (isolated from Bali, Indonesia). The relative gene expression of SOCS-3, TLR-3, NFκB, and the cytokine genes (interleukin (IL)-6, IL-8, interferon inducible protein 10 (IP-10), and macrophage inflammatory protein-1 beta (MIP-1β)) were measured using qRT-PCR at 6, 12 and 24 hours post infection (hpi). Student t-test and Mann-Whitney test were used to compare the gene expressions while causal correlations were analyzed using regression test and path analyses. DENV-2 infection increased the gene expression of SOCS-3, TLR-3, and NFκB after 12 and 24 hpi. The expression of IL-6, IL-8, IP-10, and MIP-1β genes was increased and peaked at different times post-infection. NFκB and SOCS-3 genes likely have role in the upregulation of IL-8 and IL-6 gene expression, respectively. MIP-1β gene expression was significantly induced by both NFκB and SOCS-3. In conclusion, our study suggested that SOCS-3, TLR-3, and NFκB are important in regulating the production of IL-6, IL-8, IP-10, MIP-1β during early phase of DENV-2 infection. This enriches our understanding on pathogenesis pathway of DENV-associated cytokine storm.
Co-Authors Ade Rochaeni Agung Dwi Laksono Agung Dwi Wahyu Widodo Agus Santosa Agus Santosa Agustinus Vincent Anggoro DB Sachro Arfijanto, Muhammad V. Arfijanto, Vitanata Aryati , Aryati Aryati Aryati Aryati Aryati Aryati Aryati Aryati Aryati Asmarawati, Tri Pudy Baehaqi Benediktus Yohan Benediktus Yohan Bramanton Bramantono Bramantono, Bramantono Brian Eka Rahman Dicky Febrianto Didik Hasmono Djazuly Chalidyanto Eddy Soewandojo Eko Budi Koendhori, Eko Budi Erika Marfiani Erni J. Nelwan Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Erwin Astha Triyono Fendy Suhariadi Florentina Sustini Gondo Mastutik Hardiono Hardiono, Hardiono Hari Paraton Haryo N. Yahya Helmia Farida Hening Laswati Putra Herawati Y Heru Suswojo Hidajat, Boerhan Insani Budiningsih Iqhrammullah, Muhammad Isidro Lumanpauw Ismi Masyithah Jaap M. Middeldorp Jeine Stela Akualing Juanita, Lydia Kuntaman Kuntaman Kuntaman Kuntaman Laila Hayati, Laila Lestari, Evi Noerista M Vitanata M.M. Hapsari Ma'rifah, Nurul Margalin, Brilliant Margyaningsih, Nur Ita Marinda Dwi Puspitarini Mariyatul Qiptiyah, Mariyatul Mei Wulan, Sri Mardjiati Monique Keuter Muchlis AU Sofro Muchlis AU. Sofro Muhammad Iqhrammullah Muhammad Miftahussurur Muhammad Vitanata Arfijanto, Muhammad Vitanata Muhammad Vitanata Arifijanto Musofa Rusli Nasronudin Nasronudin Nenci Siagian Nurul Ma'rifah P. Fans P.J,van den Broek Pathak, Yashwant Prihartini Prihartini Purnomo, Windu Puspa Wardhani Puspa Wardhani Puspitarini, Marinda Dwi R. Tedjo Sasmono R. Tedjo Sasmono Rachmad Suhanda Rachman, Brian Eka Ronald Pratama Adiwinoto Sasmono, R. Tedjo Setyarini, Wahyu Sholeh, Mohammad Akbaruddin Sofro, Muchlis AU Sofro, Muchlis AU. Sri Masyeni, Dewa Ayu Putri Sripatmi Sripatmi, Sripatmi Suci Andriani Sugiyatmi, Tri A. Suharto Suharto Susilowati Andajani, Susilowati Tenta Hartian Hendyatama Tripudi Asmarawati Troy Fonda Wahyu Setyarini Widodo J. Pudjirahardjo Widodo Jatim Pudjirahardjo Windu Purnomo Yoes Prijatna Dachlan Yohan, Benediktus