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All Journal Jurnal Veteriner Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biomedical Engineering Indonesian Journal of Tropical and Infectious Disease WMJ (Warmadewa Medical Journal) YARSI Medical Journal Biomolecular and Health Science Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology Jurnal Keperawatan Soedirman (The Soedirman Journal of Nursing) Folia Medica Indonesiana
Retno Handajani
Department Of Biochemistry, 00Faculty Of Medicine, Universitas Airlangga, Surabaya
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : Microbiology Indonesia

 1 
The Prevalence and Subtype Distribution of Hepatitis C Virus Infection among Hemodialysis Patients in a Private Hospital in Surabaya, Indonesia MOCHAMAD AMIN; JUNIASTUTI .; TAKAKO UTSUMI; YOSHIHIKO YANO; MOCHAMAD YUSUF; MOCHAMMAD THAHA; PRIYO BUDI PURWONO; RETNO HANDAJANI; SOETJIPTO .; HAK HOTTA; YOSHITAKE HAYASHI; MARIA INGE LUSIDA
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.539 KB) | DOI: 10.5454/mi.6.4.5

Abstract

The prevalence of hepatitis C virus (HCV) infection has been as high as approximately 80% in patients with maintenance hemodialysis in public hospitals in Indonesia. However, the prevalence in private hospitals has not been examined yet. The aim of  this study was  to  investigate  the prevalence of anti-HCV antibody and  the subtype distribution in patients with hemodialysis in a private hospital in Surabaya, Indonesia. Sera were obtained from 41 hemodialysis patients in a private hospital in Surabaya. The positive prevalence of anti-HCV antibody was carried out by  the enzyme-linked  immunosorbent assay (ELISA). Anti-HCV-positive sera were subjected  to  reverse transcription-PCR (RT-PCR) to detect HCV RNA and then direct sequencing. The HCV subtype was examined by phylogenetic analysis. Twenty five patients (61%) out of 41 were positive for anti-HCV antibody, and HCV-RNA was detected in 19 patients. The positive prevalence of anti-HCV antibody was 7.7% (one out of 13 patients) among patients who had undergone hemodialysis  for  less  than one year, whereas  it was 85.7%  (24 out of  28  patients)  among  patients who  had  undergone  hemodialysis  for  over  one  year.  Phylogenetic  analysis revealed HCV-1a  (52.6%) was  the most common  subtype,  followed by 1b  (15.8%), 1c  (15.8%), 2a  (5.3%), and 3k (5.3%). In conclusion,  the prevalence of HCV  infection among hemodialysis patients  in a private hospital was as high as  that  in general hospitals. The predominant subtype was HCV-1a, which  is  in accordance with  the previous studies  in general hospitals  in Surabaya,  Indonesia.
Genotype of Hepatitis B Virus Coinfection in Typhoid Patients SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.254 KB) | DOI: 10.5454/mi.9.3.6

Abstract

Typhoid fever can cause liver disorder and may result in complication. Studies revealed hepatic involvement in thypoid known as typhoid hepatitis. Our previous paper reported the existance of hepatitis B virus (HBV) coinfection in the serum of patients with typhoid using nested-polymerase chain reaction (PCR). Based on sequence divergence, HBV has been classified into 10 genotypes (A-J), which in prediction response and correlates with clinical outcome of chronic HBV infection. This study was conducted to determine the genotypes of HBV in typhoid patients coinfected with HBV in Tuban. Sera obtained from 5 typhoid patients positive HBV by nested PCR). Study was performed by direct sequencing BigDye V1.1 Terminator Cycle Sequencing kit and ABI Prism 310 Genetic Analyzer. Analys had been using the Genetix version 10 software to create the phylogenetic tree. Phylogenetic analysis showed 3 samples as genotype B and 2 two samples as genotype C.
Prevalence of Hepatitis B Virus Infection in Blood Donors Based on Titer Hepatitis B Surface Antigen Examination (HBsAg) SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.189 KB) | DOI: 10.5454/mi.13.4.5

Abstract

Hepatitis B remains a global public health problem. Infection from hepatitis B virus (HBV) can be transmitted through a blood test or a blood transfusion. This study was conducted to identify the prevalence of HBV infection in blood donors based on examination of HBsAg titers . Blood donors from Tuban Red Cross used as sample. The method used in this research is HBsAg titers examination performed by ELISA according to the procedureoutlined in the Kit. HBsAg titers positive mostly found in men. In men from 13 samples (8.67%) are HBsAg titers positive of 150 samples while in woman all negative for HBsAg titers from 137 samples. The average titer positive was 3.095 with a standard deviation of 0.187. While HBsAg titers negative have average of 0.03 with a standard deviation of 0.14. This study showed that the prevalence of HBV infection in blood donors is most numerous in men with HBsAg titers positive number of 8.67%.
Co-Authors A Kentjono, Widodo Adi, Ginanjar Sasmito Afrina Zulaikah Amin, Indah Binti Mohd Anggraeni, Zuhrotul Eka Yulis Ayesie Natasha Zulka B Notopuro, Paulus Bakhtiar, Arief Bakhtiar, Arief Bayu Satria Wiratama David S. Perdanakusuma Edhi Rianto Elvaretta, Clarissa Ely Rahmatika Nugrahani Ely Rahmatika Nugrahani, Ely Rahmatika Fis Citra Ariyanto Gatut Suhendro Ginanjar Sasmito Adi HAK HOTTA Harianto Notopuro Hendri Susilo Hendy Muagiri Margono Henydhar Bramastivira Mahdani Hernomo Ontoseno Kusumobroto Humairah, Ira I Gusti Agung Komang Diafari Djuni Hartawan Indri Safitri Indri Safitri, Indri Juniastuti Juniastuti Kawilarang, Arthur Pohan Khaerunnisa, Siti Luki Indriaswati Lukitasari, Lina Lukitasari, Lina Margarita Maramis, Margarita MARIA INGE LUSIDA Maria Inge Lusida MOCHAMAD AMIN MOCHAMAD AMIN Mochamad Amin Mochamad Yusuf Alsagaff Mochammad Amin Mochammad Thaha MOCHAMMAD THAHA Nurwasis Nurwasis Nyilo Purnami Palupi, Dian Eka Roro Poernomo Boedi Setiawan Prabowo, Gwenny Ichsan Pranawa Pranawa Priyo Budi Purwono Qurnianingsih, Ema Radias Dwi Padmani Rizaliyana, Sitti Ruby Riana A Ruby Riana Asparini Ruslan, S. Eriaty N. S. Perdanakusuma Samira Assegaf Siti Khaerunnisa SOETJIPTO SOETJIPTO . SOETJIPTO . Soetjipto Soetjipto Sulistyo Mulyo Agustini Supiana Dian Nurtjahyani Susi Wahyuning Asih Susi Wahyuning Asih, Susi Wahyuning Suwandito Suwandito Suwandito, Suwandito Syafa'ah, Irmi Syafa’ah, Irmi TAKAKO UTSUMI Ulfa Kholili Ummah, Fithriyah Cholifatul Ummi Maimunah Waskito, Langgeng Agung Wiwik Misaco Yuniarti Wungu, Citrawati Dyah Kencono Wungu, Citrawati Dyah Kencono YOSHIHIKO YANO YOSHITAKE HAYASHI Yulianti, Erikavitri Zain Budi Syulthoni Zuhrotul Eka Yulis Anggraeni Zulka, Ayesie Natasha