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ROLE OF MYCORRHIZA HELPER BACTERIA ON MYCORRHIZAL COLONIZATION AND NEMATODE Pratylenchus coffeae INFECTION Hindersah, Reginawanti; Lilipaly, Elena F. L.; Asyiah, Iis Nur; Mudakir, Imam; Harni, Rita
BIOTROPIA Vol. 29 No. 3 (2022): BIOTROPIA Vol. 29 No. 3 Desember 2022
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2022.29.3.1711

Abstract

The coffee nursery is susceptible to endoparasitic Pratylenchus coffeae. Application of biological method in the nursery is suggested to control the nematode population and maintain the seedling health. The objectives of this study were to observe the ability of Arbuscular Mycorrhiza Fungi (AMF) Glomus spp. and liquid inoculant of Mycorrhiza Helper Bacteria (MHB) consortium Pseudomonas diminuta and Bacillus subtilis for increasing AMF colonization and reducing the infection P. coffeae in Arabica coffee seedling and their growth. A pot experiment was conducted using a Completely Randomized Block Design with four treatments and five replications. The treatments were Glomus spp. spore inoculation without and with two concentrations of MHB. The control treatment did not receive Glomus spp. and MHB. The seedlings were growing in the greenhouse for three months. The results indicated that Glomus spp. and MHB consortium significantly reduced the nematode total number in soil and roots by approximately 30%; and infection degree of P. coffeae by 50%. The application of Glomus spp. significantly increased root colonization by mycorrhizal fungi, but MHB inoculation did not affect the mycorrhizal colonization. Seedlings treated with MHB had higher shoot length compared to the plant without MHB and control; but the leaves number and shoot dry weight of seedlings were not affected by all treatments. Even though the root fresh weight was reduced after MHB treatment, the lateral roots growth of MHB-treated seedling visually was improved. The experiment demonstrated that MHB was efficient to reduce P. coffeae infection of Arabica seedling.
Cow Manure for Improving Carbon-to-Nitrogen Ratio of Tailings, Growth, and Biomass of Water Kale (Ipomoea reptans Poir.) Hindersah, Reginawanti; Ardelia, Zahra; Sofyan, Emma Trinurani; Setiawan, Ade; Damayanti, Farida
Jurnal Ilmu Pertanian Vol 10, No 3 (2025): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.106456

Abstract

Gold mine tailings are characterized by low organic matter, poor nutrient availability, extreme pH conditions, and unfavorable texture, all of which restrict plant growth. This study evaluated the effects of cow manure (CM) on soil chemical properties, carbon-to-nitrogen (C/N) ratio, and the growth and biomass of water kale (Ipomoea reptans Poir.) cultivated in tailings-based media. A greenhouse experiment was arranged in a randomized block design consisting of five treatments: 0% (control), 15%, 30%, 45%, and 60% CM (v/v), each replicated six times. Observations included soil pH, organic carbon, total nitrogen, C/N ratio, plant growth parameters, biomass, and microbial populations at 35 days after planting. The application of CM significantly increased organic C, total N, and the C/N ratio, with the highest values observed at 60% CM. However, higher CM doses reduced substrate pH to slightly acidic levels. The 15% CM treatment produced the greatest improvements in plant growth and biomass, increasing plant height by 21%, stem thickness by 29%, leaf number by 8%, and fresh and dry biomass by 73% and 120%, respectively, compared with the control. Increasing CM above 15% enhanced the C/N ratio but did not further improve plant performance, likely due to reduced nitrogen availability associated with higher organic matter and slower mineralization. CM applications did not significantly affect bacterial or fungal populations in the rhizosphere. Overall, the findings indicate that low-dose CM (15%) is optimal for improving the C/N ratio, supporting nutrient availability, and enhancing water kale growth in tailings. This study demonstrates the potential of organic amendments for rehabilitating gold mine tailings and highlights their role in restoring soil functionality and supporting sustainable revegetation efforts.
Cow Manure for Improving Carbon-to-Nitrogen Ratio of Tailings, Growth, and Biomass of Water Kale (Ipomoea reptans Poir.) Hindersah, Reginawanti; Ardelia, Zahra; Sofyan, Emma Trinurani; Setiawan, Ade; Damayanti, Farida
Jurnal Ilmu Pertanian Vol 10, No 3 (2025): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.106456

Abstract

Gold mine tailings are characterized by low organic matter, poor nutrient availability, extreme pH conditions, and unfavorable texture, all of which restrict plant growth. This study evaluated the effects of cow manure (CM) on soil chemical properties, carbon-to-nitrogen (C/N) ratio, and the growth and biomass of water kale (Ipomoea reptans Poir.) cultivated in tailings-based media. A greenhouse experiment was arranged in a randomized block design consisting of five treatments: 0% (control), 15%, 30%, 45%, and 60% CM (v/v), each replicated six times. Observations included soil pH, organic carbon, total nitrogen, C/N ratio, plant growth parameters, biomass, and microbial populations at 35 days after planting. The application of CM significantly increased organic C, total N, and the C/N ratio, with the highest values observed at 60% CM. However, higher CM doses reduced substrate pH to slightly acidic levels. The 15% CM treatment produced the greatest improvements in plant growth and biomass, increasing plant height by 21%, stem thickness by 29%, leaf number by 8%, and fresh and dry biomass by 73% and 120%, respectively, compared with the control. Increasing CM above 15% enhanced the C/N ratio but did not further improve plant performance, likely due to reduced nitrogen availability associated with higher organic matter and slower mineralization. CM applications did not significantly affect bacterial or fungal populations in the rhizosphere. Overall, the findings indicate that low-dose CM (15%) is optimal for improving the C/N ratio, supporting nutrient availability, and enhancing water kale growth in tailings. This study demonstrates the potential of organic amendments for rehabilitating gold mine tailings and highlights their role in restoring soil functionality and supporting sustainable revegetation efforts.
Co-Authors A.M. Kalay Abraham Talahaturuson, Abraham Adawiah, Aliya Zahrah Adawiyah, Aliya Z Ade Setiawan Ade Setiawan Adita Rizky Syntianis Aditya, Fasa Aditya, Fasa Aditya, Fasa Agnia Nabila Agung Karuniawan Agung Muhammad Yusuf Agus Jacob Ai Siti Santriyani Aisyah, Ayu Siti Akustu, Masako Alia Halimatusy Aliya Zahrah Adawiah Alyani Shabrina amiruddin teapon Ane Nurbaity Ani Yuniarti Anne Nurbaity ANNE NURBAITY Anni Yuniarti Anny Yuniarti Antonio Yusuf Ardelia, Zahra Ariani, Nabila Syifa Arief Harsono Asep Purnama Hidayat Ashwath, Nanjappa Asmiran, Priyanka Aten Komarya Bagus Adityo Bang, Sunbaek Bari, Ichsan Nurul Benito R. Kurnani Benny Joy Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Bina Nugraha, Gita Chotimah, Andina D Herdiyantoro D. A Sulaksana Dadang Epi Sukarsa Damayani, Maya Dedeh H. Arief Dedeh Hudaya Arief Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedi Nursyamsi Dedy Nursyamsi Dewi, Yeni Wispa Dewikusuma Ikhsani Dewikusuma Ikhsani Adhiningtyas Diana Nafitri Cahayaningrum Diky Indra Wibawa Dirga Sapta Sara Diyah Sri Utami Diyan Herdiyantoro Diyan Herdiyantoro DIYAN HERDIYANTORO Dwi Suci Rahayu Emma Trinurani Sofyan Endo, Kenji Erni Suminar Erwin Erwin Etty Pratiwi Farida Damayanti Fauzul Amri Febby Nur Indriani Febby Nur Indriani Fera Siti Meilani Ferra Langoi Fiky Yulianto Wicaksono Fiky Yulianto Wicaksono Fitriatin, Betty Fitriatin, Betty N Fitriatin, Betty Natalie Gina Nurhabibah H Yulina Handayani, Sri Harry Rum Haryadi Harsono, dan Arief Henry Kesaulya Herman Rehatta Hidiyah Ayu Ratna Ma’rufah Ibnu Haikal Iis Nur Asyiah Iis Nur Asyiah Imam Mudakir Imran Makatita Indra Herliana Irene A. Ngabalin Ishak, Lily Jajang Sauman Hamdani James Matheus June A Putinella June Putinella Juniart Leklioy Kaffah, Ruhnayati Latifah, Tipah Lilipaly, Elena F. L. Lukman Gunarto M Riadh Uluputty Maharani, Nadhira Saniya Mahdi Argawan Putra Mahfud Arifin Mardiyani Sidayat Marenda Ishak Sonjaya Sule Marina Jamlean Martha Fani Cahyandito Matsuura, Asana Maulana, Haris Maulana, Nurzen Mauludy, Noor Muhammad Maya Damayani Mayang Agustina Meddy Rachmadi Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mudiyati Rahmatunnisa Mulyadi Mulyadi Nadia Nuraniya Kamaluddin Nakayama, Masakazu Nandha Afrilandha NANJAPPA ASHWATH Neni Rostini NENNY NURLAENY Nenny Nurlaeny Nia Kurniati Nia Kurniati Nia Kurniati Ninda Meiditia Putri Nini Mila Rahmi Nizar Ulfah Nono Carsono Nugraha, Gita Bina Nugraha, Gita Bina Nugraha, Gita Bina Nurfitriana, Nofalia Nurmayulis Nuryani, dan Oktaviani, Meira Osok, Rafael M Oviyanti Mulyani Priyanka Asmiran Priyanka Asmiran PRIYANKA PRIYANKA Probo Condrosari Pudjawati Suryatmana Pujawati Suryatmana Puji Syara Anggia Putri Sri Judiani Purba Rachelita, Nadia Rachmat Harryanto Rahmatika Risanti, Rara Rara Rahmantika Risanti Rhasajati, Dhia Rhazista Noviardi Rhazista Noviardi Riadh Uluputty Rija Sudirja Rina Devnita Risanti, Rara Risanti, Rara Rahmatika Risanti, Rara Rahmatika RITA HARNI Rita Harni Robby Risamasu Robi Risamasu Rochimi Setiawati, Mieke Rudi Priyadi Rustam Rustam Salsabilla, Camilla Sandra Amalia Riyadi Sandrawati, Apong Saon Banerjee Sarita Sarkar Setiawati, Mieke Setiawati, Mieke Richimi Setiawati, Mieke Rochimi Shabrina Rahma Fauzia Soetijoso Soemitro Solihin, Amir Sondi Kuswaryan Suman Samanta Sumbada, Rifyal Achmad Rivaldi Sunardi Sunardi Sunarya, Yaya - Tarkus Suganda Toto Sunarto Tri Hanggono Achmad Tri Mulya Hartati Triani Dewi Triani Dewi Triyani Dewi Triyani Dewi Triyani Dewi Tualar Simarmata Vera Oktavia Subarja Vira Kusuma Dewi Wahyuda Rachman, Wahyuda Wawan Kurniawan Wawan Rachman Wilhelmina Rumahlewang Wilhelmina Rumahlewang Wilhemina Rumahlewang Yansen Lakburlawal Yuliati Mahfud Yusi Firmansyah Yusuf Rahman Yusup Hidayat Zellya Handyman