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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sains Peternakan Jurnal Sain Peternakan Indonesia Jurnal Peternakan Indonesia Jurnal Ternak Tropika Livestock and Animal Research Tropical Animal Science Journal JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Peternakan Nusantara JURNAL KAJIAN VETERINER Jurnal Ilmu Peternakan Terapan Journal of Tropical Animal Science and Technology Wahana Peternakan Jurnal Nukleus Peternakan COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Jurnal Peternakan Lahan Kering Jurnal Sosial dan Sains Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan Lentera: Multidisciplinary Studies Journal of Livestock and Animal Health (JLAH) Animal Agricultura Lentera: Multidisciplinary Studies
Thomas Mata Hine
Fakultas Peternakan, Kelautan Dan Perikanan, Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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EVALUASI KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER SPERMAX MODIFIKASI DENGAN WAKTU PRE-FREEZING YANG BERBEDA (Assessment of Landrace Boar Frozen Semen Quality in Modified Spermax Diluent Under Varying Pre-freezing Durations) Kii, Mariana B.I.; Hine, Thomas M; Bette, Yustiany Y; Nalley, Wilmientje M
JURNAL NUKLEUS PETERNAKAN Vol 12 No 1 (2025): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v12i1.22385

Abstract

Research was conducted to investigate the impact of different pre-freezing durations (PF) using a modified spermax (M) extender on the quality of frozen landrace boar semen. The study utilized fresh semen from four healthy Landrace boars aged 2-3 years. Fresh semen meeting SNI standards was diluted in SM extender and held at room temperature (27-28℃) for two hours. Subsequently, the semen was centrifuged at 2000 rpm for 10 minutes, the supernatant was discarded, and the sperm pellet was re-diluted with SM extender at a 1:1 ratio. The semen was then packaged in 0.5 mL straws, placed on a freezing rack, and allowed to equilibrate for two hours at 3-5℃. Following equilibration, the semen was re-evaluated, and the straws were frozen above liquid nitrogen at -110℃ with varying pre-freezing times of 5, 8, 11, and 14 minutes. After the pre-freezing period, the straws were plunged into liquid nitrogen for final freezing and then stored in liquid nitrogen containers at -196℃. The results indicated that the duration of pre-freezing time did not lead to statistically significant differences (P>0,05) in sperm motility, viability, abnormality, and recovery rate (RR). In conclusion, pre-freezing times ranging from 5 to 14 minutes had a similar impact on the quality of frozen landrace boar semen.
Perbandingan Kualitas Spermatozoa Babi Landrace dalam Pengencer Dasar Natrium Chloride Fisiologis dengan Level Kuning Telur yang Berbeda Ina Mato, Sefania; Nalley, W. Marlene; Mata Hine, Thomas; Marawali, Aloysius
COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat Vol. 4 No. 1 (2024): COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/comserva.v4i1.1292

Abstract

Penelitian ini dilakukan dengan tujuan untuk mengetahui level optimal kuning telur (KT) dalam pengencer natrium chloride fisiologis (NaClF) untuk preservasi spermatozoa babi landrace. Materi penelitian adalah semen yang berkualitas baik (motilitas spermatozoa ?70%, abnormalitas spermatozoa <20%) yang ditampung dari dua ekor babi jantan landrace, yang berumur ± dua tahun. Rancangan yang digunakan adalah rancangan acak lengkap yang terdiri dari enam perlakuan, yakni: P0: NaClF, P1: NaClF+5% KT, P2: NaClF+10% KT, P3: NaClF+15% KT, P4: NaClF+20% KT, P5: NaClF25% KT. Setiap perlakuan mendapat ulangan lima kali. Variabel yang diukur dalam penelitian ini adalah motilitas, viabilitas, abnormalitas, dan daya tahan hidup spermatozoa. Hasil penelitian ini menunjukkan bahwa hingga jam ke-24 preservasi, penambahan KT 5-25% ke dalam pengencer NaClF menghasilkan motilitas dan daya tahan hidup spermatozoa yang lebih tinggi daripada kontrol (P<0,05), namun untuk variabel viabilitas dan abnormalitas spermatozoa menunjukkan pengaruh yang tidak signifikan (P>0,05). Disimpulkan bahwa level optimal KT dalam pengencer NaClF untuk preservasi spermatozoa babi landrace adalah 20%
Pengaruh kombinasi pengencer Air Kelapa Muda dan Beltsville Thawing Solution Terhadap Kualitas Spermatozoa Babi Landrace Doda Dengi, Donatus; Mata Hine, Thomas; Kune, Petrus
COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat Vol. 4 No. 3 (2024): COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/comserva.v4i3.1378

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi pengencer air kelapa muda (AKM) dan Beltsville Thawing Solution (BTS) terhadap kualitas semen babi landrace. Penelitian ini menggunakan semen segar yang berkualitas baik dengan motilitas sperma ? 70% dan abnormalitas < 20%. Semen ditampung dari seekor ternak babi landrace jantan yang berumur dua tahun dan telah mencapai dewasa kelamin. Semen tersebut diencerkan dengan pengencer AKM dan BTS dengan komposisi perlakuan yang diuji yaitu P0= AKM, P1= AKM 75% + BTS 25%, P2= AKM 50% + BTS 50%, P3= AKM 25% + BTS 75%, P4= BTS, dan selanjutnya dipreservasi didalam coolbox yang bersuhu 18-20°C. eval__uasi kualitas sperma dilakukan setiap delapan jam sekali dengan variabel yang diukur dalam penelitian ini adalah motilitas, viabilitas, abnormalitas, dan daya tahan hidup spermatozoa. Data penelitian dianalisis menggunakan sidik ragam dan dilanjutkan dengan uji Duncan. Hasil penelitian ini menunjukkan bahwa kombinasi pengencer AKM 50 + BTS 50% (P2) dan AKM 25 + BTS 75% (P3) mempunyai kualitas semen yang lebih tinggi (P<0,05) dibandingkan ketiga perlakuan lainya. Disimpulkan bahwa kombinasi pengencer AKM dan BTS dengan perbandingan 50 : 50% dan 25: 75% efektif dalam mempertahankan kualitas semen babi landrace
PENGARUH LEVEL ZINK DALAM PENGENCER TRIS KUNING TELUR TERHADAP KUALITAS SEMEN CAIR SAPI ANGUS (The effect of zinc level in the tris egg yolk extender on the quality of angus liquid semen) Siena, Sofia M.S.; Hine, Thomas Mata; Nalley, W. Marlene; Marawali, Aloysius
JURNAL NUKLEUS PETERNAKAN Vol 11 No 1 (2024): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v11i1.8346

Abstract

Efforts to improve cattle productivity can be done by using artificial insemination (AI) technology. Many factors determine the success of AI, one of which is the diluent used in semen preservation. The purpose of this study was to determine.the effect.of zinc level in.tris-egg yolk.diluent (T-EY) on the.quality of liquid semen of Angus bull. Semen is collected once a week using.an artificial.vaginal method from a three-year-old Angus bull in an undisturbed or healthy body and reproductive organs. Semen was diluted with T-EY diluent which had been added with zinc at several levels, 0 mg control treatment (T0), 10.mg (T1), 20.mg (T2), 30.mg (T3), 40 mg (T4). The diluted semen was.stored at a temperature of 3-5oC, then observed every 24 hours for.motility, viability,.abnormalities and survival of.spermatozoa to a minimum motility of 40%. The.results showed.that the addition of 20 mg of zinc in the T-EY.diluent (T2) resulted in a higher.quality of liquid semen of Angus bull (P˂0.05) than other.treatments. The quality of the liquid semen (motility, viability, abnormalities and survival of spermatozoa) were motility 44±5.75%, viability 48.39±13.59%, abnormality 6.91±1.85% and durability.live 5.60±0.89 days. It was.concluded that the.addition.of 20 mg zinc in the.tris-egg yolk diluent produced liquid semen of Angus bull with higher quality than other treatments.
Improving The Quality Of Duroc Boars Semen Through The Application of Sun Dry Carrot Extract In Spermax-Egg Yolk Diluent Masae, Sinoptis Melkiur; Marawali, Aloysius; Hine, Thomas Mata; Kune, Petrus
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 3 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i3.p661-675

Abstract

This research aimed to determine the best level of sun-dried carrot extract (SDCE) in the spermax-egg yolk (EY-S) diluent for preserving the sperm of duroc boars. Semen was collected from two 2-year-old healthy duroc boars. The study employed an experimental method with a completely randomized design (CRD), consisting of five treatments and five replicates. The five treatments were T1 = EY-S + SDCE 10%, T2 = EY-S + SDCE 12.5%, T3 = EY-S + SDCE 15%, T4 = EY-S + SDCE 17.5%, T5 = EY-S + SDCE 20%. Semen, which was diluted with the treatment diluent, was stored in a cool box at a temperature of 18-20°C and observed every 12 hours until sperm motility reached 40%. The results indicated that treatment P5 was able to maintain the quality of liquid boar semen better than the other treatments (P<0.05) with sperm motility (46.00%), sperm viability (51.92%), sperm abnormality (4.90%), and sperm survival (66.33 hours). It was concluded that a 20% level of sun-dried carrot extract in spermax-egg yolk diluent can preserve motility for up to 60 hours of storage. This study demonstrates that dried carrot extract can enhance the quality and storage capacity of spermatozoa, making it an effective alternative in storage media. This is beneficial in animal reproduction, particularly in artificial insemination, where sperm motility is crucial for fertilization. Furthermore, this research opens up opportunities to explore other natural substances in sperm diluents, reducing reliance on synthetic chemicals and improving the sustainability of reproductive practices.
Preservasi Spermatozoa Babi Landrace Dalam Tiga Jenis Pengencer Modifikasi Papituan, Mardan Laes; Nalley, Wilmientje Marlene; Hine, Thomas Mata
TERNAK TROPIKA Journal of Tropical Animal Production Vol. 25 No. 1 (2024): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2024.025.01.1

Abstract

Pengenceran semen memiliki dua fungsi utama yaitu untuk memperpanjang masa simpan dan memperbesar volume semen. Penelitian ini bertujuan untuk mengetahui perbandingan penambahan ekstrak daun kelor (EDK) 5% dalam pengencer tris- kuning telur (T-KT), sitrat- kuning telur (S-KT) dan beltsville thawing solution®-kuning telur (BTS® -KT) terhadap kualitas semen babi landrace selama penyimpanan. Semen babi diambil seminggu sekali menggunakan metode masase dari satu ekor babi jantan berumur 2 tahun dengan kodisi sehat dan organ reproduksi yang normal. Semen yang berkualitas baik dibagi dan diencerkan dengan pengencer perlakuan yaitu : P1: T-KT – 5% EDK , P2: S-KT – 5% EDK dan P3: BTS® -KT – 5% EDK. Kemudian pengenceran semen disimpan dalam syrofoam pada suhu 18-22ᶱ C dan dievaluasi setiap 8 jam meliputi motilitas, viabilitas, abnormalitas serta membran plasma utuh (MPU). Rancangan yang digunakan adalah rancangan acak lengkap yang terdiri atas tiga perlakuan dan lima ulangan. Data yang telah diperoleh dianalisis dengan uji anova, apabila terdapat perbedaan yang nyata antar perlakuan maka dilanjutkan dengan uji duncan. Hasil penelitian menunjukkan bahwa pengencer S-KT modifkasi (P2) mempunyai kualitas yang lebih baik (P<0,05) dibandingkan dengan perlakuan lainnya yaitu selama 40 jam dengan motilitas (45,40±4,15 %), viabilitas (56,66±1,32) dan MPU (55,44±5,18%). Kesimpulan dari hasil penelitian ini yaitu pengenceran modifikasi terbaik adalah pengencer S- KT dengan penambahan EDK 5 % (P2).
Effect of Aloe vera Gel Administrated on Reproductive Performance of Female Rats G, Gunawan; T Setiatin, Enny; Rosadi, Bayu; Mata Hine, Thomas; Parakkasi, A.
Jurnal Kedokteran Hewan Vol 1, No 1 (2007): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i1.3101

Abstract

The research was conducted to find out the effect of Aloe vera gel administrated orally onreproductive performance of female rats. Fifteen two months old female rats were alloted to fivetreatments of per oral Aloe vera gel administration: P0 (control), P1(1 mg /g body weight), P2 (2 mg/gbody weight, P3 (3 mg/g body weight), and P4 ( 4 mg/g body weight). The gel were administrated everyday for four weeks. The body weight were measured on the same day as last treatment. The animal thenmated to proven male on 1:1 rasio. Positively mated animals were sacrificed on D-10 after mating tocollect uterus and ovary so the number of fetus, corpus luteum, and ovary weight can be determined. Thetreatments significantly decreased (P0.05) the average body weight gain. The P0 (4.1 g/head/day) wasdifferent to P1 (2.69 g/head/day), P2 (2.87 g/head/day, and P4 (2.68 g/head/day), but was not different toP3 (3.39 g/head/day). The ovarium weight were not different (P0.05) among the treatments, so did thenumber of corpus luteum (CL), the number of fetuses and the CL: fetus rasio.Keywords: Aloe vera, rats, reproductive performance .
Pengaruh Penambahan Ekstrak Biji Kelor Kering (Moringa oleifera Lam) dalam Pengencer Beltsville Thawing Solution terhadap Kualitas Semen Cair Babi Landrace Pandahuki, Fransiska Onya; Nalley, W. Marlene; Uly, Kirenius; Mata Hine, Thomas
Animal Agricultura Vol 2 No 1 (2024): Animal Agricultura
Publisher : Yayasan Sumber Daya Manusia Cerdas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59891/animacultura.v2i1.67

Abstract

The aim of this research was to determine the effect of adding dry moringa seed extract (DMSE) added to the Beltsville thawing solution (BTS) diluent on the quality of landrace boar semen during storage. This research used experimental methods and a completely randomized design  consisting of 5 treatments and 5 replications to form 25 experimental units. The treatment in question was : T0 = BTS EY + 0% DMSE, T1 = BTS EY + 1% DMSE, T2 = BTS EY + 2% DMSE, T3 = BTS EY + 3% DMSE, T4 = BTS EY + 4% DMSE. This diluted semen will be stored at a temperature of 18-20ºC and evaluation of motility, viability, abnormalities and survival of spermatozoa was carried out every 8 hours of storage. Data was analysis of the average, standard deviation, variance and continued with the Duncan test. The results showed that T2 treatment with a 2% DMSE level at 32 hours of storage gave the best results (P<0.05) compared to other treatments, with motility values (51, 80±4.71), viability (65.54±5.00), abnormality (5.81±0.81 and survival value of (38.89±1.21). It was concluded that the addition of EBKK at a level of 2% to the BTS diluent provided a good response in maintaining motility, viability and abnormalities.
Natural honey supplementation in Tris-egg yolk diluent as Carbohydrate source replacing Fructose in Etawah Crossbred Goat semen Bette, Yustiani Yuliana; Nalley, Wilmientje Marlen; MataHine, Thomas
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 12 No. 1 (2024)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v12i1.p49-62

Abstract

This study aims to identify the substitution of native honey as an energy source to replace fructose in Tris-KT diluent to improve the quality of spermatozoa of Etawah crossbreed goats (PE). The study was conducted at the Nekamese Vocational School and the Wiliams and Laura Foundation Biological and Reproductive Laboratory for 2 months. In this study, four PE male goats were used as a source of semen. Cement is accommodated twice a week. The semen obtained was evaluated macroscopically and microscopically then diluted with T-KT diluent with the addition of honey divided into P0 (control), P1 (T-KT + 0.25 w/v honey), P2 (T-KT + 0.50 w/v honey) and P3 (T-KT + 0.75 w/v honey). After dilution the cement is stored at a temperature of 3-5°C.  Semen is evaluated post-dilution and post-storage every 24 hours, until the movement of spermatozoa is at least 40%. The variables tested were motility and viability of spermatozoa. P3 provides the best results in maintaining cement quality. The results showed that honey substitution in Tris-KT diluent exerted a marked effect on the motility and viability of spermatozoa (P<0.05) during storage.
Kualitas Spermatozoa Babi Landrace dalam Pengencer Sitrat-Kuning Telur dengan Substitusi Sari Buah Melon (Cucumis melo L) Kurnia Wawang, Silviani; Nalley, Marlene; Mata Hine, Thomas
COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat Vol. 3 No. 11 (2024): COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/comserva.v3i11.1248

Abstract

Semen babi pada dasarnya bersifat produktif dengan kosentrasi yang rendah, hal ini berpengaruh terhadap kelebihan semen setelah penampungan. Penelitian ini bertujuan untuk menguji efektivitas sari buah melon-kuning telur (SBM-KT) dalam pengencer sitrat-kuning telur (S-KT) terhadap kualitas spermatozoa babi landrace. Bahan yang digunakan adalah semen segar babi landrace kualitas baik yang diencerkan dengan pengencer perlakuan sebagai berikut: P0: S-KT 100%, P1: S-KT 90% + SBM-KT 10%, P2: S-KT 80% + SBM-KT 20%, P3: S-KT 70% + SBM-KT 30%, P4: S-KT 60% + SBM-KT 40%. Semen yang telah diencerkan disimpan pada suhu 18-20o C dan dievaluasi setiap 8 jam terhadap motilitas, viabilitas, abnormalitas dan daya tahan hidup spermatozoa. Hasil penelitian menunjukkan bahwa kualitas spermatozoa dalam pengencer sitrat-kuning telur tanpa penambahan sari buah melon (P0) memperlihatkan hasil lebih tinggi dibandingkan dengan perlakuan lainnya dengan motilitas (45,00±1,76), viabilitas (55,86±1,11), abnormalitas (4,55±0,54) dan daya tahan hidup (45,30 jam). Dari data diatas maka dapat disimpulkan bahwa substitusi sari buah melon dalam pengencer sitrat-kuning telur tidak efektif untuk mempertahankan kualitas spermatozoa babi landrace. Kesimpulan penelitian ini menunjukkan bahwa pengencer dengan komposisi yang berbeda dapat memengaruhi kualitas spermatozoa selama penyimpanan, dan perlakuan tanpa penambahan sari buah melon memiliki efek yang lebih baik dalam mempertahankan motilitas, viabilitas, abnormalitas, dan daya tahan hidup spermatozoa babi Landrace.
Co-Authors A. Kaka A. Parakkasi A. Parakkasi Abdullah Baharun Adyanto Nessy Banamtuan Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Aloysius Marawali Arief Boediono Ate, Desmon Gono Ayang C. Tafuli Bayu Rosadi Burhanuddin * Burhanuddin . C. Tafuli, Ayang Djawapatty, D. J. Doda Dengi, Donatus Donatus Doda Dengi Dondin Sajuthi Dui, Theresia A. A. T. Enny T Setiatin Enny T Setiatin, Enny Franky M S Telupere George Valentino Kosat Grasele, Fransiskus Allianus Gue, Febronia Dhenge Gunawan G Gunawan G, Gunawan Henderiana Belli Henderiana L.L Belli Heri Armadianto Heri Armadianto Hilly, Fanny Tesalonika Honin, Olfi Mariana Iman Supriatna Ina Mato, Sefania J A Tamoes Jernih Blegur Johanis Ly Kihe, Johny Nada Kii, Mariana B.I. Kirenius Uly kirenius uly Kirenius Uly Kirenius Uly Kristina Meldiana Hayati Kune , P. Kurnia Wawang, Silviani L. L Belli, Henderiana L. L Belli, Henderiana Laka, Theresia Oktaviana Lawa , A. B. Lawa, Alvrado B Lawa, Alvrado Bire Listyadi, Rut marabijala, jourvan manasye Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Masae, Sinoptis Melkiur Nalley, Marlene Nalley, W Marlene Nalley, W. Marlene Nalley, Welmince M. Nalley, Wilmientje M Nalley, Wilmientje M. Nalley, Wilmientje Marlen Nana, Arinda E. Nduang, Helena Reslina Ni Made Paramita Setyani Nuba, Maria Deran Odo, Remigius Abha Pandahuki, Fransiska Onya Papituan, Mardan Laes Petrus Kune Petrus Kune Petrus Kune Petrus Kune Petrus Kune Riwu , R. M. J. Riwu, Agustinus R. Riwu, Agustinus Ridlof Riwu, Roni Markus Jami Samuel Alexsander Singga Sefania Ina Mato Selsiana Dongkot Setyani , N. M. P. Setyani, Ni M P Siena, Sofia M.S. Simon Edison Mulik Sofia Marlize Soleman Manaze Weiri Sinda Stef Amiron Ratu Tako, Alfonia Inna Tapatab, Kelvin Alberto Imanuel Telupere, Franky M S Toto, Novaldy Imanuel Tree May Thiessen Outang Twen O. Dami Dato Ulu, Krista Florida Uly , K. Uly, Kirenius W. M. Nalley W. Marlene Nalley Wilmientje M. Nalley Wilmientje Marlen Nalley Wilmientje Marlene Mesang Nalley Wilmientje Marlene Nalley, Wilmientje Marlene Wilmintje Marlene Nalley Wirona Adu Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette Yusuf Sae Zulfa, Yanna Nailla