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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sains Peternakan Jurnal Sain Peternakan Indonesia Jurnal Peternakan Indonesia Jurnal Ternak Tropika Livestock and Animal Research Tropical Animal Science Journal JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Peternakan Nusantara JURNAL KAJIAN VETERINER Jurnal Ilmu Peternakan Terapan Journal of Tropical Animal Science and Technology Wahana Peternakan Jurnal Nukleus Peternakan COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Jurnal Peternakan Lahan Kering Jurnal Sosial dan Sains Jurnal Ilmiah Ilmu-Ilmu Peternakan Jurnal Kedokteran Hewan Lentera: Multidisciplinary Studies Journal of Livestock and Animal Health (JLAH) Animal Agricultura Lentera: Multidisciplinary Studies
Thomas Mata Hine
Fakultas Peternakan, Kelautan Dan Perikanan, Universitas Nusa Cendana
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Improvement of the Quality of Landrace Pig Spermatozoa in Diluent of Young Coconut Water and Tris Combination C. Tafuli, Ayang; Mata Hine, Thomas; Marawali, Aloysius
Jurnal sosial dan sains Vol. 4 No. 5 (2024): Jurnal Sosial dan Sains
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/jurnalsosains.v4i5.1307

Abstract

ABSTRACT Background: The research material used was fresh semen of 2-year-old landrace boars of good quality (spermatozoa motility ≥ 70% and spermatozoa abnormality < 20%) Purpose: This study aimed to find the best combination of young coconut water (YCW) and Tris to improve the quality of landrace boars spermatozoa. Method: %). This study used an experimental method according to a complete randomized design procedure with five treatments and five replicates. The treatments were: T0= YCW, T1= TCW 75% + tris 25%, T2= YCW 50% + tris 50%, T3= YCW 25% + tris 75%, T4= Tris. All treatment diluents were added with 20% egg yolk, 3% moringa leaf extract, 1,000 IU/mL penicillin, and 1,000 µg/mL streptomycin. The variables tested were motility, viability, abnormality, and survival. Results: The observation results at the 40th hour of storage showed that treatment T3 was significantly different (P<0.05) on motility, viability, abnormality and survival but on the variable viability treatment T3 was insignificantly different from T1 (p>0.05), but significantly different from treatment T0, T2, and T4; and for spermatozoa abnormality variables in all treatments were the same (p>0.05) Conclusion: It was concluded that the combination of YCW 75% and tris 25% and 100% tris effectively maintained the quality of landrace boars spermatozoa
Efek Penambahan Kuning Telur Pada level Yang Berbeda Dalam Pengencer Spermax Terhadap Kualitas Semen Beku Babi Landrace: Effect of Adding Different Levels of Egg Yolk in Spermax Diluent on The Quality of Landrace Boars' Frozen Semen Tako, Alfonia Inna; Nalley, W Marlene; Setyani, Ni Made Paramita; Hine, Thomas Mata
Jurnal Ilmiah Ilmu-Ilmu Peternakan Vol 28 No 2 (2025): November 2025
Publisher : Fakultas Peternakan Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiiip.v28i2.44806

Abstract

Background: The benefits of frozen semen can improve the genetic quality of the offspring, the wide distribution of superior genetics to various locations even in distant areas. Purpose: The purpose of this study was to determine the optimal level of egg yolk in spermax diluent for the preservation of frozen semen of landrace boars. Methods: The design used was a completely randomized design consisting of four treatments with four replications each, namely: P1 = Spermax + 5% egg yolk, P2 = Spermax + 10% egg yolk, P3 = Spermax + 15% egg yolk, and P4 = Spermax + 20% egg yolk. Each treatment diluent was supplemented with 6% glycerol. Semen was diluted in the base diluent and stored for 2 hours at room temperature (27–28 °C). Next, the semen was centrifuged at 2000 rpm for 10 minutes, the supernatant was discarded, and the sediment was re-diluted. The semen was then evaluated for sperm motility, viability, and abnormalities, packaged in 0.5 mL straws, and equilibrated for 2 hours at a temperature of 3–5 °C. The semen was re-evaluated and frozen on the surface of liquid nitrogen at a distance of 10 cm for 10 minutes, then stored in a container filled with liquid nitrogen for 24 hours. The frozen semen was subsequently evaluated for motility, viability, abnormalities, and recovery rate. Results: The results of the study did not have a significant effect (T> 0.05) with the highest values in each treatment after thawing being motility (T3 = 28.75 ± 11.08%), viability (T4 = 47.52 ± 18.14%), abnormality (T1 = 9.84 ± 2.05%) and recovery rate (T3 = 35.93%). Conclusion: Addition of egg yolk with levels of 5%, 10%, 15% and 20% in the spermax diluent produces the same quality of frozen landrace boar semen.
Efek Sari Daun Sereh Wangi Terhadap Kualitas Semen Babi Landrace X Duroc Dalam Pengencer Tris-Kuning Telur marabijala, jourvan manasye; Nalley, Wilmientje Marlene; Hine, Thomas Mata; Lawa, Alvrado Bire
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.21196

Abstract

The purpose of this research is to evaluate the level of lemongrass leaf extract added to Tris-egg yolk diluent to ensure that the spermatozoa quality of landrace x duroc crossbred boars is maintained during storage. In this study, fresh semen was used from 1.5 yearsold landrace x duroc crossbred boars that were in healthy condition and had been trained to collect semen. To conduct this research, completely randomized design was used, consisting of six treatments and five replications, namely: T0= Tris-egg yolk, T1= Tris-egg yolk + lemongrass leaf extract 0,5%, T2= Tris-egg yolk + lemongrass leaf extract 1,0%, T3= Tris-egg yolk + lemongrass leaf extract 1,5%, T4= Tris-egg yolk + lemongrass leaf extract 2,0%, T5= Tris-egg yolk + lemongrass leaf extract 2,5%. After dilution, the semen was stored in a styrofoam box at a temperature of 18-20°C. Four variables were tested: motility, viability, abnormality, and sperm survival. These variables were analyzed using a variability test and Duncan's analysis. The findings revealed that the spermatozoa quality of treatment P2 at the 60th hour of observation displayed a notable distinction. (P<0.05) from other treatments. The motility value was 54.00%, the viability was 64.10%, the abnormality was 5.70%, and the sperm survival was 67.68 hours. This study found that adding lemongrass leaf extract to Tris-egg yolk diluent at a level of 1.0% improved the quality of landrace x duroc crossbred boars spermatozoa.
Synergistic Impact of Cholesterol-Loaded Cyclodextrin and Moringa Leaf Extract on Post-Thaw Boar Sperm Kinematics Hine, T. M.; Nalley, W. M.; Uly , K.; Kune , P.; Lawa , A. B.; Setyani , N. M. P.; Bette , Y. Y.; Banamtuan , A. N.; Riwu , R. M. J.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.30

Abstract

Structural and functional damage during cryopreservation usually impairs the quality of boar sperm, especially post-thaw motility and kinematic performance, which restricts its use in artificial insemination programs. Cholesterol-loaded cyclodextrin (CLC) and moringa leaf extract (MLE) are considered possible cryoprotective additives because they are membrane-stabilizing and antioxidants. The study investigated the possible synergistic effect of the Tris-egg yolk (TY) extender in combination with CLC and MLE on the motility and kinematics properties of thawed boar sperm, as identified in a Computer-Assisted Sperm Analysis (CASA) system. Ejaculates of four healthy boars were diluted with Tris-based extender without or with CLC (1 or 2 mg/mL), MLE (1 or 1.5 mg/mL), or both, and frozen with a conventional protocol. Kinematic variables alongside progressive and fast motility were included as post-thaw tests. The findings showed that combination treatment of 2 mg/mL CLC and 1 mg/mL MLE (T7) resulted in a significant increase in all sperm motility and kinematic variables compared to the control group (T0) (p<0.05). In particular, T7 had better progressive motility (38.04%), fast motility (22.42%), and higher kinematic variables, especially VCL (106.34 µm/s), VSL (36.28 µm/s), and VAP (45.41 µm/s), and better displacement and trajectory indices. T7 was always better than the single supplementation and other combinations. Finally, the addition of CLC (2 mg/mL) and MLE (1 mg/mL) into the Tris egg yolk extender could be a successful approach towards optimizing semen cryopreservation in boars.
PENGARUH KOMBINASI FRUKTOSA DAN SUKROSA DALAM PENGENCER TRIS-KUNING TELUR TERHADAP KUALITAS SEMEN CAIR BABI LANDRACE: Influence of Combination of Fructose and Sucrose in Diluent Tris-Yolk Against Quality of Liquid Landrace Boars Semen Samuel Alexsander Singga; Wilmintje Marlene Nalley; Alvrado Bire Lawa; Thomas Mata Hine
Wahana Peternakan Vol. 9 No. 3 (2025): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v9i3.6835

Abstract

The purpose of this study was to examine the effect of the combination of fructose (F) and sucrose (S) in egg yolk Tris-diluent (T-EY) on the quality of Landrace boars semen. This study used an experimental method in a complete randomized design consisting of 5 treatments and 5 repeats so that 25 experimental units were formed. The treatment in question was T0= T-EY; T1= T-EY + F 1.0% + S 0.2%; T2= T-EY + F 1.5% + S 0.2%; T3= T-EY + F 1.0% + S 0.4%; T4= T-EY + F 1.5% + S 0.4%. Good quality semen was diluted with a test diluent, then preserved at a temperature of 18-20o C. The variables studied are motility, viability, abnormalities and survival of spermatozoa. This evaluation is done every 12 hours. The results showed that at 60 hours preservation of T2 treatment resulted in the highest and significantly different spermatozoa motility (P>0.05) with all treatments with spermatozoa motility values (43.00%), spermatozoa viability (43.10%), spermatozoa abnormalities (11.40%), and spermatozoa survival (63.20 hours)). It was concluded that the combination of 1.5% fructose and 0.2% sucrose at T2 in the egg yolk Tris-diluent resulted in the best liquid semen quality in maintaining Landrace pig semen quality during low-temperature storage.     Keywords: Fructose, sucrose, qualitys of semen boars Landrace, Tris-yolk egg  
Correlation Between Chest Girth, Body Length, and Body Height on Wither Weight of Rote Sheep Ewes Setyani, Ni Made Paramita; Nalley, Wilmientje Marlene; Hine, Thomas Mata; Riwu, Agustinus Ridlof; Uly, Kirenius; Kune, Petrus; Bette, Yustiany Yuliana; Lawa, Alvrado Bire; Mulik, Simon Edison; Banamtuan, Adyanto Nessy; Riwu, Roni Markus Jami; Zulfa, Yanna Nailla; Listyadi, Rut
Journal of Tropical Animal Science and Technology Vol. 8 No. 1 (2026): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v8i1.10617

Abstract

This study aimed to analyze the relationship between linear body measurements including chest girth (CG), body length (BL), and wither height (WH) and body weight (BW) in Rote sheep ewes across different age groups to determine the most reliable predictor of body weight. The research employed a quantitative method through direct field measurements and weighing. Data collected including vital statistics (CG, BL, WH) and BW from Rote sheep ewes categorized into three age groups (I0, I2, and I4). The data were analyzed statistically using correlation coefficient (r), the coefficient of determination (R²), and bias analysis to evaluate the accuracy of weight estimation. The results revealed that chest girth exhibited the highest correlation with body weight, particularly in the I0 group (r = 0.93; R² = 86.05%), indicating a strong positive relationship and high predictive accuracy. Body length showed a moderate relationship (r = 0.87; R² = 76.11%) in the same group, while wither height demonstrated a relatively weaker correlation (r = 0.73; R² = 53.94%). Across all age groups, the predictive strength of body measurements decreased with increasing age, suggesting that skeletal growth contributes less to body weight variation in mature sheep. In conclusion, chest girth was identified as the most accurate and reliable linear measurement for predicting body weight in Rote sheep ewes, particularly in younger age groups, and can therefore be used as a practical and non-invasive tool for estimating livestock weight.
Kualitas Semen Beku Babi Landrace dalam Pengencer Vitasem dengan Level Kuning Telur yang Berbeda Nana, Arinda E.; Kune, Petrus; Riwu, Agustinus Ridlof; Hine, Thomas M.
Jurnal Sains Peternakan Vol 13 No 2 (2025): Jurnal Sains Peternakan Vol. 13 No. 2
Publisher : Fakultas Peternakan, Universitas PGRI Kanjuruhan Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jsp.v13i2.12177

Abstract

Penelitian ini bertujuan menguji kualitas semen beku babi landrace dalam pengencer vitasem dengan level kuning telur yang berbeda. Rancangan yang digunakan dalam penelitian adalah Rancangan Acak Lengkap. Dengan lima perlakuan dan empat ulangan, dengan perlakuan terdiri atas (P1) vitasem + 5% kuning telur, (P2) vitasem + 10% kuning telur, (P3) vitasem + 15% kuning telur, (P4) vitasem + 20% kuning telur, (P5) vitasem + 25% kuning telur. Semen diencerkan, dikemas dalam straw 0,5 ml, lalu diekuilibrasi pada 3-5℃ selama dua jam sebelum dibekukan di atas nitrogen cair         (-110℃) selama 10 menit. Selanjutnya, straw dicelupkan ke dalam nitrogen cair dan disimpan dalam wadah khusus (goblet, kanister, kontainer) pada -196℃.Variabel yang diamati meliputi motilitas, viabilitas, abnormalitas, dan recovery rate spermatozoa. Data dianalisis dengan ANOVA dan dilanjutkan DMRT. Hasil menunjukkan bahwa perlakuan tidak signifikan pada tahap pra-pembekuan, namun signifikan setelah thawing (P<0,05). Perlakuan P5 menghasilkan rata-rata tertinggi untuk motilitas (32,38%), viabilitas (58,51%), dan recovery rate (40,54%), sedangkan abnormalitas terendah (10,27%) ada pada P3. Penelitian ini menyimpulkan bahwa level kuning telur yang berbeda efektif mempertahankan motilitas spermatozoa pasca-thawing, dengan P5 memberikan hasil terbaik dengan motilitas ≥30%
Kualitas Semen Beku Babi Landrace dalam Pengencer Sitrat Kuning Telur dengan Waktu Pre Freezing yang Berbeda Nduang, Helena Reslina; Riwu, Agustinus Ridlof; Kune, Petrus; Hine, Thomas Mata
Jurnal Sains Peternakan Vol 13 No 2 (2025): Jurnal Sains Peternakan Vol. 13 No. 2
Publisher : Fakultas Peternakan, Universitas PGRI Kanjuruhan Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jsp.v13i2.12178

Abstract

Penelitian ini bertujuan untuk menemukan lama waktu pre freezing sperma dalam pengencer sitrat kuning telur (S-KT). Penelitian ini menggunakan semen segar dari 4 ekor ternak babi jantan landrace yang telah mencapai dewasa kelamin dengan umur ternak babi adalah 2-3 tahun dan dalam keadaan sehat. Penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri dari empat perlakuan dan empat ulangan dengan waktu pre freezing yakni: P1 = Sitrat kuning telur + gliserol 6% (3 menit), P2 = S-KT 6% (6 menit), P3 = S-KT 6% (9 menit), P4 = S-KT 6% (12 menit). Semen yang telah diencerkan dalam pengencer sitrat kuning telur disimpan pada sushu ruang 27-28℃ selama 2 jam (holding time). Selanjutnya disentrifugasi selama 15 menit dengan kecepatan 3000 RPM, supernatan dibuang dan pelet diencerkan kembali menggunakan pengencer S-KT. Kemudian semen dikemas dalam straw 0,5 ml dilanjutkan dengan packing dan disusun dalam rak pembekuan dan diekuilibrasi dalam suhu 3-5℃ selama 2 jam. Setelah itu pembekuan semen di atas permukaan uap N2 cair dengan waktu pre freezing 3, 6, 9, dan 12 menit dan disimpan dalam kontainer N2 cair (-196℃). Hasil penelitian ini menunjukkan bahwa lama waktu pre freezing tidak berpengaruh nyata (P> 0,05) terhadap motilitas, viabilitas, abnormalitas dan recovery rate (RR). Dapat disimpulkan bahwa waktu pre freezing yang dilakukan selama 3 hingga 12 menit tidak memberikan dampak terhadap kualitas semen beku babi landrace
Efek Suplementasi Sari Buah Timun (Cucumis sativus) dalam Pengencer Tris-Kuning Telur terhadap Kualitas Spermatozoa Babi Persilangan Landrace X Duroc Laka, Theresia Oktaviana; Hine, Thomas Mata; Marawali, Aloysius; Nalley, Wilmientje Marlene
Jurnal Sains Peternakan Vol 13 No 2 (2025): Jurnal Sains Peternakan Vol. 13 No. 2
Publisher : Fakultas Peternakan, Universitas PGRI Kanjuruhan Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jsp.v13i2.12201

Abstract

Penelitian ini bertujuan untuk mengetahui efek penambahan sari buah timun (SBT) dalam pengencer Tris-kuning telur (T-KT) terhadap kualitas spermatozoa babi persilangan landrace x duroc. Materi penelitian ini menggunakan semen segar ternak babi persilangan landrace x duroc dengan umur 1,5 tahun dan dalam keadaan sehat. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap yang terdiri dari 6 perlakuan dan 5 ulangan sehingga mendapatkan 30 unit percobaan. Adapun perlakuan tersebut adalah: T-KT (P0), T-KT+SBT 2% (P1), T-KT+SBT 4% (P2), T-KT+SBT 6% (P3), T-KT+SBT 8% (P4), T-KT+SBT 10% (P5). Seluruh perlakuan disimpan di dalam cool box suhu 15-20ºC dan dievaluasi setiap 12 jam. Variabel yang diamati dalam penelitian ini yaitu: motilitas, viabilitas, abnormalitas dan daya tahan hidup spermatozoa. Pada jam ke-48 penyimpanan berpengaruh nyata (P<0,05) menghasilkan nilai motilitas spermatozoa 55,00%, viabilitas spermatozoa 67,50%, abnormalitas spermatozoa 4,70%, dan daya tahan hidup spermatozoa 56,24 jam. Disimpulkan bahwa penambahan sari buah timun 8 persen ke dalam pengencer Tris-kuning telur dapat menghasilkan kualitas semen babi persilangan landrace x duroc yang lebih tinggi dari perlakuan lainnya
Aplikasi Sari Lidah Buaya dalam Pengencer Sitrat-Kuning Telur untuk Meningkatkan Kualitas Semen Babi Persilangan Landrace-Duroc Ate, Desmon Gono; Marawali, Aloysius; Hine, Thomas Mata; Riwu, Agustinus Ridlof
Jurnal Sains Peternakan Vol 13 No 2 (2025): Jurnal Sains Peternakan Vol. 13 No. 2
Publisher : Fakultas Peternakan, Universitas PGRI Kanjuruhan Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jsp.v13i2.12234

Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan sari lidah buaya (SLB) pada pengencer sitrat-kuning telur (S-KT) terhadap kualitas semen babi persilangan landrace-duroc. Penelitian ini menggunakan semen segar yang di koleksi dari satu ekor babi persilangan landrace-duroc yang berumur 1,5 tahun dan dalam keadaan sehat. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap (RAL) yang terdiri dari enam perlakuan dan lima ulangan sehingga terdapat tiga puluh unit percobaan. Adapun perlakuan tersebut adalah: S-KT (P0), S-KT+SLB 2% (P1), S-KT+SLB 4% (P2), S-KT+SLB 6% (P3), S-KT+SLB 8% (P4), S-KT+SLB 10% (P5). Seluruh perlakuan disimpan dalam cool box dengan suhu 15-20ºC. Evaluasi motilitas, viabilitas, abnormalitas dan daya tahan hidup spermatozoa dilakukan setiap 12 jam. Hasil penelitian ini menunjukkan bahwa perlakuan P2 dengan SLB 4% pada jam penyimpanan ke-48 memberikan hasil terbaik (P 0,05), dengan nilai motilitas spermatozoa 51,00%, viabilitas spermatozoa 64,10%, abnormalitas spermatozoa 5,90%, dan daya tahan hidup spermatozoa 54,60 jam. Disimpulkan bahwa penambahan sari lidah buaya ke dalam pengencer sitrat-kuning telur dapat mempertahankan kualitas semen babi persilangan landrace-duroc yang lebih optimal, dengan level sari lidah buaya terbaik adalah 4%
Co-Authors A. Kaka A. Parakkasi A. Parakkasi Abdullah Baharun Adyanto Nessy Banamtuan Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Aloysius Marawali Arief Boediono Ate, Desmon Gono Ayang C. Tafuli Bayu Rosadi Burhanuddin * Burhanuddin . C. Tafuli, Ayang Djawapatty, D. J. Doda Dengi, Donatus Donatus Doda Dengi Dondin Sajuthi Dui, Theresia A. A. T. Enny T Setiatin Enny T Setiatin, Enny Franky M S Telupere George Valentino Kosat Grasele, Fransiskus Allianus Gue, Febronia Dhenge Gunawan G Gunawan G, Gunawan Henderiana Belli Henderiana L.L Belli Heri Armadianto Heri Armadianto Hilly, Fanny Tesalonika Honin, Olfi Mariana Iman Supriatna Ina Mato, Sefania J A Tamoes Jernih Blegur Johanis Ly Kihe, Johny Nada Kii, Mariana B.I. Kirenius Uly Kirenius Uly Kirenius Uly kirenius uly Kristina Meldiana Hayati Kune , P. Kurnia Wawang, Silviani L. L Belli, Henderiana L. L Belli, Henderiana Laka, Theresia Oktaviana Lawa , A. B. Lawa, Alvrado B Lawa, Alvrado Bire Listyadi, Rut marabijala, jourvan manasye Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Masae, Sinoptis Melkiur Nalley, Marlene Nalley, W Marlene Nalley, W. Marlene Nalley, Welmince M. Nalley, Wilmientje M Nalley, Wilmientje M. Nalley, Wilmientje Marlen Nana, Arinda E. Nduang, Helena Reslina Ni Made Paramita Setyani Nuba, Maria Deran Odo, Remigius Abha Pandahuki, Fransiska Onya Papituan, Mardan Laes Petrus Kune Petrus Kune Petrus Kune Petrus Kune Petrus Kune Riwu , R. M. J. Riwu, Agustinus R. Riwu, Agustinus Ridlof Riwu, Roni Markus Jami Samuel Alexsander Singga Sefania Ina Mato Selsiana Dongkot Setyani , N. M. P. Setyani, Ni M P Siena, Sofia M.S. Simon Edison Mulik Sofia Marlize Soleman Manaze Weiri Sinda Stef Amiron Ratu Tako, Alfonia Inna Tapatab, Kelvin Alberto Imanuel Telupere, Franky M S Toto, Novaldy Imanuel Tree May Thiessen Outang Twen O. Dami Dato Ulu, Krista Florida Uly , K. Uly, Kirenius W. M. Nalley W. Marlene Nalley Wilmientje M. Nalley Wilmientje Marlen Nalley Wilmientje Marlene Mesang Nalley Wilmientje Marlene Nalley, Wilmientje Marlene Wilmintje Marlene Nalley Wirona Adu Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette Yusuf Sae Zulfa, Yanna Nailla