Article Per Year (5 Year)
p-Index From 2021 - 2026
3.533
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences VALENSI JURNAL KIMIA SAINS DAN APLIKASI Jurnal Ilmu dan Teknologi Hasil Ternak The Journal of Experimental Life Sciences (JELS) Journal of Degraded and Mining Lands Management Indonesian Green Technology Journal Jurnal Pembangunan dan Alam Lestari Natural B The Journal of Pure and Applied Chemistry Research Journal of Mathematical and Fundamental Sciences JURNAL PENGABDIAN KEPADA MASYARAKAT Science and Technology Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry JMM (Jurnal Masyarakat Mandiri) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat JKPK (Jurnal Kimia dan Pendidikan Kimia) Jurnal Pengabdian kepada Masyarakat Nusantara
Arie Srihardyastutie
Chemistry Department Brawijaya University
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

Published : 39 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 39 Documents
Search

 1   2   3   4 
Training on Bioconversion of Coffee Pulp Waste into Vegan Leather Raw Material in Dusun Krajan, Tegalharjo, Glenmore, Kabupaten Banyuwangi Ardyati, Tri; Mora, Giral Baines; Permatasari, Dewi; Maulidiyah, Nuris Shobah; Wahyudi, Aleyda Nur Halizah; Srihardyastutie, Arie; Poerwadi, Bambang; Zubaidah, Elok
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 11 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i11.9473

Abstract

Banyuwangi is the largest coffee plantation centre in East Java, with robusta and arabica coffee production reaching 10,518 tonnes per year. Coffee plantation waste, which is abundant, is known as coffee pulp. Coffee pulps can be converted into organic compost/fertilizer, animal feed, and eco-friendly products such as cellulose-based nata/membrane. The abundant availability of coffee pulp waste in Banyuwangi Regency has not been correctly handled or processed to prevent environmental impact. Converting coffee pulp waste into eco-friendly products will benefit the surrounding community and increase the added value of the waste. To overcome these problems, it is necessary to engage in activities involving the local community, local government, and agro-tourism actors through counseling and training on processing coffee pulp waste/bioconversion into eco-friendly products, thereby benefiting the community in Banyuwangi Regency, especially in the Glenmore area. Training participants were given a questionnaire for assessment. Based on the results of the questionnaire analysis, it is known that the participants' understanding before the activity is: 40% of participants were very poorly understood, 13% were poorly understood, 20% were quite understood, 13% understood, and 13% were very familiar with the material of processing coffee pulp waste. After receiving training, 27% of participants achieved an excellent understanding, 33% had a moderate understanding, and 33% had a somewhat understanding. Additionally, there were still participants who did not understand as much as 7%. Based on the participants' assessment, 67% felt that the benefits of this activity were excellent and should be carried out on an ongoing basis.
Bioremediation of the Lapindo mud contaminated soil using eco-enzyme: Case of a total petroleum hydrocarbon removal Sulistiyaningrum, Dwi Rakhmatun; Rumhayati, Barlah; Srihardyastutie, Arie
Journal of Degraded and Mining Lands Management Vol. 13 No. 1 (2026)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2026.131.9659

Abstract

This research investigated the effectiveness of bioremediation of Lapindo mud-contaminated soil using an eco-enzyme for total petroleum hydrocarbons (TPH) removal. The soil samples were collected from Glagaharum and Gedang Villages, Sidoarjo, East Java, Indonesia, which have been contaminated by the Lapindo mud containing TPH. The bioremediation experiment was carried out ex situ in a container filled with contaminated soil samples, which were watered with eco-enzymes daily. Bioremediation time was optimized at 0, 7, 4, 21, and 28 days while the eco-enzymes concentration was varied at 0, 5, 10, and 15% (v/v). The concentration of TPH in the soil samples was analyzed using the gravimetric method at 0, 7, 4, 21, and 28 days. The results showed that TPH concentration was gradually decreased during remediation. The remediation removal percentage reached an optimum after 21 days. The TPH was optimally removed using the eco-enzyme at 15% (v/v), achieving 95.39%. The bioremediation of contaminated soil using eco-enzymes also increased the contents of total C and total N by 50.97% and 39.43%, respectively. However, this treatment reduces total P by 50.89%. Furthermore, the pH and temperature values for the three samples before and after the bioremediation process remained fairly stable, within the pH range of 6-7 and the temperature range of 25.7-26.2 °C.
Characterization of TPO Enzyme and its Immunogenecity in New Zealand White Rabbits (Oryctolagus cuniculus) Devi Ramadani; Andreas Budi Wijaya; Arie Srihardyastutie; Dyah Kinasih Wuragil; Rulli Rosandi; Achmad Rudijanto; Aulanni'am Aulanni'am
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9539

Abstract

Hypothyroidism is characterized by inadequate production of thyroid hormones, chiefly thyroxine (T4) and triiodothyronine (T3). Thyroid peroxidase (TPO), is an enzyme and crucial for producing thyroid hormones, and also serves as a primary autoantigen in autoimmune thyroid conditions, such as Hashimoto's thyroiditis and Graves' disease. The existence of anti-thyroid peroxidase antibodies (anti-TPO) indicates an autoimmune response in which the immune system TPO which is normally recognized as part of the body (self), begins to be identified by the immune system as a something foreign (non-self). This study aimed to characterize, measure TPO activity, and evaluate the immunogenicity of TPO protein derived from patient serum and its ability to induce TPO antibody production in New Zealand White Rabbits. TPO protein isolation was achieved through SDS-PAGE and confirmed by western blot technique have molecular weight of 52 kDa. In samples from normal patients, the average TPO enzyme activity was 31.34 U/mL, while in hypothyroid patients, the average activity increased to 49.46 U/mL. TPO polyclonal antibody production in rabbits indicated its immunogenicity, peaking in week eight post-immunization, with an ELISA absorbance of 0.555. Dot blot analysis further confirmed anti-TPO sensitivity at various dilutions, anti-TPO has a high sensitivity at 1:80 dilution still detects TPO in sera patient.
Pemberdayaan Desa Sidorahayu Kabupaten Malang melalui Inovasi Eco Enzim Safitri, Anna; Srihardyastutie, Arie; Sutrisno, Sutrisno; Aulanni'am, Aulanni'am; Sukardi, Ernawati; Yusrina, Nur; Wasino, Wasino
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 7 No. 1 (2026): Edisi Januari - April
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v7i1.8457

Abstract

Desa Sidorahayu, Kecamatan Wagir, Kabupaten Malang menghadapi permasalahan pengelolaan sampah organik rumah tangga yang belum optimal, khususnya limbah kulit buah dan sayuran yang berpotensi mencemari lingkungan. Keterbatasan pengetahuan dan keterampilan masyarakat dalam mengolah sampah menjadi produk bernilai guna menjadi permasalahan krusial yang memerlukan solusi aplikatif dan berkelanjutan. Program ini menawarkan penerapan Teknologi Tepat Guna (TTG) berupa inovasi eco enzim sebagai solusi pengelolaan sampah organik sekaligus upaya pemberdayaan masyarakat. Kegiatan dilaksanakan melalui tahapan sosialisasi, pelatihan, demonstrasi, praktik langsung, serta pendampingan produksi dan pemanfaatan eco enzim hingga tingkat dusun. Mahasiswa KKN Tematik berbobot 4 SKS dilibatkan sebagai fasilitator untuk mempercepat transfer pengetahuan dan meningkatkan partisipasi warga. Hasil evaluasi kegiatan semakin menegaskan efektivitas program, dengan 90% peserta menilai proses pembuatan eco enzim sangat mudah dan 88% menyatakan kegiatan ini sangat bermanfaat, hal ini menunjukkan tingkat penerimaan yang sangat baik sekaligus mengindikasikan peluang keberlanjutan program di tingkat komunitas. Selain itu, terbentuk kelompok warga pengelola eco enzim yang mulai memanfaatkan produk untuk kebutuhan rumah tangga dan kebersihan lingkungan. Program ini menunjukkan bahwa inovasi eco enzim efektif sebagai pendekatan pemberdayaan masyarakat dalam pengelolaan sampah organik secara berkelanjutan.
Dietary Rice Bran Plays A Significant Role in the Hepatoprotective Effect in Hypercholesterolemic Rats Antula, Yulianti; Neno, Dolly Irnawati; Srihardyastutie, Arie; Mahdi, Chanif
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1016.513 KB) | DOI: 10.21776/ub.jels.2019.009.02.08

Abstract

Cholesterol is obtained through biosynthesis and diet. When a level of cholesterol is above the normal level, this condition caused hypercholesterolemia. Long-term administration of synthetic chemical drugs can cause liver damage. Therefore, alternative natural medication is needed. One of the alternatives that can be used is the rice bran (RB), which contains antioxidant and crude fiber. This study is aimed at finding out the potential utilization of RB on total cholesterol level, liver enzyme as aspartate aminotransferase (AST) and alanine aminotransferase (ALT), malondialdehyde (MDA), and the changes in liver tissue. This study uses five groups of rats: the negative control group, positive control group, and three therapy groups with the dosage of 270 mg kg-1, 540 mg kg-1, and 810 mg kg-1 of body weight. This study shows that therapy using RB can significantly decrease the cholesterol level, AST, ALT, and MDA (p<0.01). The total cholesterol level is 21%, AST and ALT activities can be reduced to 54% and 64%, the level of MDA reduced to 79% and can repair the liver tissue. This study shows that RB can be effectively used as hepatoprotective in rats with hypercholesterolemia. Keywords: AST and ALT, Hypercholesterolemia, Rice Bran, Total Cholesterol Level.
Mikroenkapsulasi Berbasis Gum Arabik dari Ekstrak Air Pletekan (Ruellia tuberosa L.) dan Aktivitas Inhibisi Terhadap Alpha Amilase Gunawan, Firza Rajasa; Srihardyastutie, Arie; Roosdiana, Anna; Safitri, Anna
Indonesian Green Technology Journal Vol. 11 No. 2 (2022): Indonesian Green Technology Journal
Publisher : Sekolah Pascasarjana, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.igtj.2022.011.02.05

Abstract

Tanaman pletekan (Ruellia tuberosa L.) diketahui memiliki kandungan metabolit sekunder seperti flavonoid, fitosterol, sehingga diusulkan memiliki aktivitas biologis sebagai inhibitor enzim alpha-amilase. Mikroenkapsulasi merupakan metode yang dapat melindungi serta mengontrol pelepasan senyawa aktif. Penelitian ini bertujuan untuk melakukan fabrikasi terhadap ekstrak air pletekan dengan menggunakan bahan penyalut gum Arabik dan menguji aktivitasnya sebagai inhibitor untuk enzim alpha-amilase, serta mengetahui karakter mikrokapsul ekstrak air pletekan yang dihasilkan. Mikroenkapsulasi dilakukan pada variasi pH (3, 4, 5, dan 6) dan waktu pengadukan (30, 60, 90, dan 120 menit). Kondisi optimum mikrokapsul ditentukan berdasarkan penentuan efisiensi mikroenkapsulasi. Hasil penelitian menunjukkan bahwa pH 5 merupakan kondisi optimum dengan nilai efisiensi mikroenkapsulasi sebesar 51,3%. Sedangkan waktu pengadukan 90 menit merupakan kondisi optimum dengan nilai efisiensi mikroenkapsulasi sebesar 52,7%. Uji aktivitas inhibisi enzim alpha-amilase pada mikrokapsul dilakukan pada kondisi optimum menghasilkan nilai IC50 sebesar 71,61 µg/mL.. Karakterisasi menggunakan spektrofotometer FTIR menghasilkan bilangan gelombang 3401,51 cm-1 (O-H), 1608,77 cm-1 (C=C), dan 1081,07 cm-1 (C-O-C) yang menandakan terbentuknya mikrokapsul ekstrak air pletekan. Sedangkan hasil karakterisasi menggunakan SEM menunjukkan bahwa permukaan mikrokapsul yang dihasilkan masih heterogeny, dengan kecenderunagn berbentuk bulat, dan menghasilkan ukuran yang beragam berkisar antara 0,933 – 3,08 µm. Kata kunci: alpha-amilase, flavonoid, mikroenkapsulasi, pH, R. tuberosa L., waktu pengadukan
The Activity of Lipase From Jatropha Seed (Jatropha Curcas L.) And Its Application On Hydrolysis of Castor Oil In Organic Solvent Faradis, Taritsu Hazal; Srihardyastutie, Arie; Iftitah, Elvina Dhiaul
Indonesian Green Technology Journal Vol. 11 No. 2 (2022): Indonesian Green Technology Journal
Publisher : Sekolah Pascasarjana, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.igtj.2022.011.02.02

Abstract

Jatropha curcas L. is one of the seed plants that have lipase activity and can work well in hydrolysis reactions and chemical synthesis. This study aims to determine lipase activity that reacts with castor oil in organic solvents. In this research, organic solvents, especially hydrophobic solvents, were used in the hydrolysis reaction to increase lipase's catalytic activity. The organic solvent used has a hydrophobicity level between 2 and 4, namely hexane. The research stages consisted of lipase isolation, lipase assay, the effect of adding metal ions assay, and analysis of the compound of castor oil. The results showed that jatropha seeds had lipase activity in crude of 0.603 U/mL and increased to 0.911 U/mL after the addition of K+. The result of hydrolysis and transesterification of castor oil by lipase is ricinoleic acid (4,58%) and methyl ricinoleic (11,67%), and the concentration of ricinoleic acid (17.09%) and methyl ricinoleic (60.83%) were increased after addition of K+. The esterification reaction produces alkyl ester compounds such as methyl palmitate, ethyl pentadecanoate, methyl linoleate, methyl oleate, and methyl stearate. The lipase isolated from jatropha seeds not only catalyzes the hydrolysis reaction but also catalyzes chemical synthesis reactions such as esterification and transesterification. Keywords: lipase enzyme, Jatropha seeds, isolation, biocatalyst, hexane.
Hibridisasi Sintesis dan Uji Aktivitas Antikanker Turunan Baru Asam Karboksilat secara In-Silico dan In-Vitro Asyfariatus Zulfa Azhar; Warsito Warsito; Arie Srihardyastutie
Jurnal Penelitian Pendidikan IPA Vol 9 No 9 (2023): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i9.3439

Abstract

Design of new compounds as active ingredients of drugs must be selective and efficient to achieve therapeutic efficacy and minimize resulting side effects. Hybridization synthesis approaches using major components of essential oils can reliably generate new molecules that are superior as active ingredients of anti-cancer drugs due to their cytotoxic properties. This study synthesized a hybrid molecule of citronellyl salicylate by an esterification reaction using Steglich and Fisher method under ultrasonic assistance. The Steglich esterification is more efficient in producing citronellyl salicylate during 30 minutes reaction, yield 12.45%. Analysis of the synthesized product by FTIR is characterized by the presence of typical absorptions of the ester group at ῡ 1650 cm-1 and 1270 cm-1, while LC ESI-MS shows m/z 294 is indicated [M+NH4]+. Anticancer activity was tested in-silico for protein receptors MMP-9, MMP-2, Cyclin-A, p53, and BAK using Molecular Docking Pyrx 9.0 and the highest activity was shown binding affinity value -8.4 kcal/mol for the MMP-9 protein receptor. Similarly, the results of the in vitro activity assay of citronellyl salicylate to 4T1 breast cancer cells showed that the morphology of cancer cells was damaged and the viability of cancer cells was lower than that of normal cells
Studi Pemanfaatan Pupuk Organik Cair dari Air Lindi TPA Tlekung Kota Batu dengan menggunakan Ecoenzyme sebagai Biodegradasi Logam Berat (Pb, Cu) Luluk Indri Astuti; Arie Srihardyastutie; Qonitah Fardiyah
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.3466

Abstract

Leachate is water produced from the waste processing process at the final disposal site (TPA). Leachate contains various organic substances, so many local people use leachate as fertilizer. However, on the other hand, leachate contains heavy metals. As an effort to reduce the content of heavy metals such as Pb and Cu, biological remediation techniques are used by adding ecoenzymes. This study aims to determine the effectiveness of ecoenzymes on leachate at the Tlekung TPA, Batu City. Leachate at the inlet point of Tlekung Landfill, Batu City was taken as much as 20 liters and the volume of ecoenzyme was varied, namely P1 (leachate 1000 ml), P2 (leachate 1000 ml + 1 ml ecoenzyme), P3 (leachate 1000 ml + 5 ml ecoenzyme), P4 (leachate 1000 ml + 10 ml ecoenzyme), P5 (leachate 1000 ml + 15 ml ecoenzyme). The research stages were carried out by testing the pH using a pH meter, testing the number of colonies using the Total Plate Count (TPC) method, testing the levels of lead (Pb) and copper (Cu) using the AAS method. The results showed that the addition of ecoenzyme to leachate affected the pH value, increased the number of colonies and decreased the levels of Pb and Cu metals. The addition of 10 mL of ecoenzyme in 1000 ml of leachate in the P4 treatment could increase the number of colonies and reduce the levels of Pb and Cu.
Co-Authors Achmad Rudijanto Agustina Shinta Hartati Wahyuningtyas Akhmad Sabarudin Alyaa Farrah Dibha Andreas Budi Wijaya Angelina Rosmawati Ani Mulyasuryani Anisa Aprilia Anna Roosdiana Anna Roosdiana Anna Roosdiana Anna Roosdiana Antula, Yulianti Asyfariatus Zulfa Azhar Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Aulanni'am, Aulanni'am Aulanni`am Aulanni`am Aulanni’am Aulanni’am Aulanni’am Aulanni’am Aulanni’am Aulanni’am Aulanni’am, Aulanni’am Azor Yulianus Tefa Bambang Poerwadi Barlah Rumhayati Barlah Rumhayati Barlah Rumhayati Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Danar Purwonugroho Danar Purwonugroho Destyana Ellingga Pratiwi Destyana Ellingga Pratiwi Devi Ramadani Dewi Permatasari, Dewi Diah Mardiana Dyah Kinasih Wuragil Elok Zubaidah Elvina Dhiaul Iftitah Endah Setiani Astuti Eny Rahma Wati Eva Nur Laili Octaviana Fahmi, Ahmad Luthfi Fahriyah Fahriyah Faijal Fachrudin Mbabho Faradis, Taritsu Hazal Fiddaroini, Saidun Fitria Rahmawati Gunawan, Firza Rajasa Hani Susanti, Hani Janatun Na’imah Karti'a, Galuh Wahyu KIKI FIBRIANTO Komara, Acep Nana Latifah Tribuana Dewi Luluk Indri Astuti Made Oka Adnyana Made Oka Adnyana Maria Natalia Wewo Masruri Masruri Maulidiyah, Nuris Shobah Moh. Farid Rahman Mora, Giral Baines Muhammad Asadullah Muhammad Iman Hidayat, Muhammad Iman Nabila, Sofi Naila Azmi Adiba Neno, Dolly Irnawati Nur Aeni Nur Lailah Nur Samsu Pertiwi, Vi’in Ayu Pertiwi, Vi’in Ayu Putranty Widha Nugraheni Qonitah Fardiyah Riawati Riawati Riyanti Isaskar Roja’i, Abdul Munir Rulli Rosandi Rurini Retnowati Safitri, Anna Salsabila, Isadora Evani Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Siti Nurul Afifah Sofi Nabila Sukardi, Ernawati Sulistiyaningrum, Dwi Rakhmatun Sutrisno Sutrisno Sutrisno, Sutrisno Tri Ardiyati Tri Ardyati Tri Ardyati Velayaty Labone Azzahra Vi’in Ayu Pertiwi Wahyudi, Aleyda Nur Halizah Warsito Warsito Warsito Warsito Wasino Wasino Yudita Prihatini Puji Rahma Sari Yuniar Ponco Prananto Yusri Fajar Yusrina, Nur