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All Journal HAYATI Journal of Biosciences VALENSI JURNAL KIMIA SAINS DAN APLIKASI Jurnal Ilmu dan Teknologi Hasil Ternak The Journal of Experimental Life Sciences (JELS) The Indonesian Green Technology Journal Natural B The Journal of Pure and Applied Chemistry Research Journal of Mathematical and Fundamental Sciences JURNAL PENGABDIAN KEPADA MASYARAKAT Science and Technology Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry JMM (Jurnal Masyarakat Mandiri) JKPK (Jurnal Kimia dan Pendidikan Kimia)
Arie Srihardyastutie
Chemistry Department Brawijaya University
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences Other

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Nanoparticle-Enhanced 3D-Connector Microfluidic Paper-Based Analytical Device (3D-µPADs) for Sensitive and Cost-Effective Detection of Albumin-Creatinine Ratio in Urine Sample Sabarudin, Akhmad; Fiddaroini, Saidun; Fahmi, Ahmad Luthfi; Roja’i, Abdul Munir; Salsabila, Isadora Evani; Aulanni’am; Srihardyastutie, Arie; Susanti, Hani; Samsu, Nur
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.504-518

Abstract

Chronic kidney disease (CKD) is a global health challenge affecting over 800 million people worldwide. Early detection is crucial to prevent progression to end-stage renal disease (ESRD), where life-saving interventions like dialysis or transplantation are necessary. Among the markers for early kidney damage, the Albumin Creatinine Ratio (ACR) in urine is one of the most reliable. Conventional methods of ACR detection, such as LC/MS-MS and ELISA, are highly accurate but require expensive equipment and skilled personnel, limiting their accessibility, especially in resource-limited settings. To address this, we developed a 3D-connector microfluidic paperbased analytical device (3D-µPADs) enhanced with gold nanoparticles (AuNPs) for sensitive and low-cost ACR detection. The integration of AuNPs amplifies colorimetric signals, enhancing the visual distinction in albumin detection. Our 3D-µPADs werefabricated using chromatographic paper Whatman No. 1 with hydrophobic barriers created by solid wax printing, followed by reagent immobilization for albumin and creatinine detection. The colorimetric and distance responses, based on reactions with Bromocresol Green (BCG) and Chrome Azurol S-Palladium (CAS-Pd2+), were analyzed using ImageJ software to quantify albumin and creatinine levels. The 3D-µPADs exhibited optimal sensitivity and accuracy, with linear detection ranges for albumin and creatinine of 30–400 mg/g. Validation with human urine samples demonstrated an accuracy of 93.04%, suggesting that 3D-µPADs offer a promising alternative for early nephropathy detection. Our findings provide a cost-effective, accessible tool for CKD screening, potentially transforming diagnostics in low-resource environments.
Studi Pemanfaatan Pupuk Organik Cair dari Air Lindi TPA Tlekung Kota Batu dengan menggunakan Ecoenzyme sebagai Biodegradasi Logam Berat (Pb, Cu) Luluk Indri Astuti; Arie Srihardyastutie; Qonitah Fardiyah
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.3466

Abstract

Leachate is water produced from the waste processing process at the final disposal site (TPA). Leachate contains various organic substances, so many local people use leachate as fertilizer. However, on the other hand, leachate contains heavy metals. As an effort to reduce the content of heavy metals such as Pb and Cu, biological remediation techniques are used by adding ecoenzymes. This study aims to determine the effectiveness of ecoenzymes on leachate at the Tlekung TPA, Batu City. Leachate at the inlet point of Tlekung Landfill, Batu City was taken as much as 20 liters and the volume of ecoenzyme was varied, namely P1 (leachate 1000 ml), P2 (leachate 1000 ml + 1 ml ecoenzyme), P3 (leachate 1000 ml + 5 ml ecoenzyme), P4 (leachate 1000 ml + 10 ml ecoenzyme), P5 (leachate 1000 ml + 15 ml ecoenzyme). The research stages were carried out by testing the pH using a pH meter, testing the number of colonies using the Total Plate Count (TPC) method, testing the levels of lead (Pb) and copper (Cu) using the AAS method. The results showed that the addition of ecoenzyme to leachate affected the pH value, increased the number of colonies and decreased the levels of Pb and Cu metals. The addition of 10 mL of ecoenzyme in 1000 ml of leachate in the P4 treatment could increase the number of colonies and reduce the levels of Pb and Cu.
Development of Fermented Coconut Water and Red Ginger using Next Generation Probiotics as a Potential Biological Activity Agent Nabila, Sofi; Srihardyastutie, Arie; Aulanni’am, Aulanni’am; Ardiyati, Tri
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1337-1351

Abstract

Degenerative diseases have increased in prevalence worldwide, predominantly in Indonesia. Some studies found that probiotics have an impact on organic substances that address inflammation and gut microbiota imbalance. This study aims to develop a functional beverage based on coconut water and red ginger fermentation using Next Generation Probiotics (NGP) and characterize the product using metagenomic and metabolomic technologies. This experimental study involved the fermentation of coconut water and red ginger using a probiotic starter identified by 16S rRNA sequencing for 120 hours at room temperature. The analysis included microbial characterization using List the probiotics metagenomic sequencing, metabolite profiling by LC-HRMS, particle size by PSA, and in vitro antibacterial and anti-inflammatory activity testing. Metagenomic analysis identified the dominance of the family Acetobacteraceae (45%) and genus Acetobacter 942%. Fermentation reduced the tannin content by 28.5% and increased the number of metabolite compounds from 37 to 54. The fermentation results also showed antibacterial activity against E. coli CNN 0091 (inhibition zone 7.835 mm), and anti-inflammatory activity reached 1,000%. The combination of coconut water, red ginger, and NGP produced a complex metabolite profile with high therapeutic potential. The findings show significant potential for the prevention of degenerative diseases.
PENDAMPINGAN ECO-LITERASI DALAM MENDUKUNG GERAKAN SEDEKAH SAMPAH INDONESIA (GRADASI) MENUJU SEKOLAH ADIWIYATA SE-KOTA MALANG Isaskar, Riyanti; Pratiwi, Destyana Ellingga; Pertiwi, Vi’in Ayu; Srihardyastutie, Arie; Fajar, Yusri
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol. 30 No. 1 (2024): JANUARI-MARET
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkm.v30i1.50400

Abstract

Permasalahan lingkungan tengah menjadi sorotan hingga kancah internasional. Adiwiyata merupakan salah satu bentuk perilaku hijau di lingkungan sekolah mengingat program adiwiyata memiliki tujuan untuk menciptakan kondisi sekolah yang baik dan nyaman melalui lingkungan sekitar sekolah. Sikap eco-literasi di tingkat sekolah dapat dipupuk dengan cara menerapkan prinsip 3R (Reduce, Reuse, dan Recycle). Prinsip Reduce (mengurangi) terdapat pada program gradasi, sedangkan Reuse dan Recycle terdapat pada program pembuatan Eco Enzyme dan produk turunannya. Gradasi merupakan program yang diadakan untuk mengurangi jumlah sampah plastik yang kian hari semakin meningkat. Kegiatan pengabdian masyarakat yang diberi nama BRILIAN dilaksanakan pada rentang waktu 27 Juni - 19 Agustus 2022. kegiatan yang melibatkan siswa, metode yang digunakan adalah presentasi dan praktik. Materi yang disampaikan diantaranya adiwiyata, GRADASI, eco-enzyme, dan kompos. Tahapan kegiatan program BRILIAN terbagi menjadi tiga tahapan yakni pra-kegiatan, kegiatan inti dan pasca-kegiatan. Pra-kegiatan berisi webinar untuk mengetahui sekolah yang belum atau sudah berpredikat adiwiyata, kegiatan inti berisi kegiatan sosialisasi dan praktik di sekolah yang melibatkan guru dan siswa dan pasca-kegiatan berisi kegiatan monitoring dan evaluasi dari kegiatan yang sudah dilakukan. 
Co-Authors Achmad Rudijanto Agustina Shinta Hartati Wahyuningtyas Akhmad Sabarudin Alyaa Farrah Dibha Andreas Budi Wijaya Angelina Rosmawati Ani Mulyasuryani Anisa Aprilia Anna Roosdiana Anna Roosdiana Anna Roosdiana Anna Roosdiana Asyfariatus Zulfa Azhar Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Aulanni`am Aulanni`am Aulanni’am Aulanni’am Aulanni’am Aulanni’am Aulanni’am Aulanni’am Aulanni’am, Aulanni’am Azor Yulianus Tefa Barlah Rumhayati Barlah Rumhayati Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Chanif Mahdi Danar Purwonugroho Danar Purwonugroho Destyana Ellingga Pratiwi Destyana Ellingga Pratiwi Devi Ramadani Diah Mardiana Dolly Irnawati Neno Dyah Kinasih Wuragil Endah Setiani Astuti Eny Rahma Wati Eva Nur Laili Octaviana Fahmi, Ahmad Luthfi Fahriyah Fahriyah Faijal Fachrudin Mbabho Fiddaroini, Saidun Firza Rajasa Gunawan Fitria Rahmawati Hani Susanti, Hani Janatun Na’imah Karti'a, Galuh Wahyu KIKI FIBRIANTO Latifah Tribuana Dewi Luluk Indri Astuti Made Oka Adnyana Made Oka Adnyana Maria Natalia Wewo Masruri Masruri Moh. Farid Rahman Muhammad Asadullah Nabila, Sofi Naila Azmi Adiba Nur Aeni Nur Lailah Nur Samsu Pertiwi, Vi’in Ayu Pertiwi, Vi’in Ayu Putranty Widha Nugraheni Qonitah Fardiyah Riawati Riawati Riyanti Isaskar Roja’i, Abdul Munir Rulli Rosandi Rurini Retnowati Safitri, Anna Salsabila, Isadora Evani Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Sasangka Prasetyawan Siti Nurul Afifah Sofi Nabila Sutrisno Sutrisno Tri Ardiyati Tri Ardyati Velayaty Labone Azzahra Vi’in Ayu Pertiwi Warsito Warsito Warsito Warsito Yudita Prihatini Puji Rahma Sari Yulianti Antula Yuniar Ponco Prananto Yusri Fajar