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All Journal Jurnal Kedokteran Brawijaya Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Kesehatan Reproduksi Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Universa Medicina Jurnal Gizi Klinik Indonesia Majalah Farmaseutik Indonesian Contemporary Nursing Journal (ICON Journal) Window of Health : Jurnal Kesehatan Jurnal Kesehatan Reproduksi Molekul: Jurnal Ilmiah Kimia Media Karya Kesehatan Journal of Community Empowerment for Health JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Proceeding ISETH (International Summit on Science, Technology, and Humanity) Prosiding University Research Colloquium Jurnal Tumbuhan Obat Indonesia Indonesian Journal of Pharmacology and Therapy

Mae Sri Hartati Wahyuningsih

*) Department Of Pharmacology And Therapy, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada; *) Herbal Medical Center, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 287503

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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The effect of a-terpineol on cell cycle, apoptosis and Bcl-2 family protein expression of breast cancer cell line MCF-7 Damiana Sapta Candrasari; Sofia Mubarika; Mae Sri Hartati Wahyuningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACT The cytotoxic activity of a-terpineol on T47D and HeLa cancer cell lines have been reported. This study was conducted to evaluate the effect of a-terpineol on cell cycle, apoptosis and Bcl-2 as well as Bax expression on MCF-7 cell line. The cytotoxic activity of a-terpineol was determined using MTT cell assay. Cell cycle and apoptosis were analysed using flowcytometry, whereas Bcl-2 and Bax expression were evaluated using immunohistochemistry. The results showed that a-terpineol had cytotoxic effect on the MCF-7 cell lines with an IC50 value of 33.0 ± 5.4 μg/mL. a-Terpineol induced cell accumulation in Sub-G1 lead to apoptosis of the MCF-7 cell. Moreover, a-terpineol inhibited Bcl-2 and induced Bax expressions. In conclusion, a-terpineol has potential anticancer activity against MCF-7 cancer cell line trough through cells cycle inhibition and apoptosis stimulation.

The effect of active compound isolated from the leaves of kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] on cell cycle and angiogenesis of WiDr cell line Hajid Rahmadianto Mardihusodo; Mae Sri Hartati Wahyuningsih; Indwiani Astuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 03 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Colorectal cancer is the tenth most common form of malignant tumor of hospital inpatients inIndonesia. Advance approaches in anticancer development is discovery molecular-targeted drugs.Molecular targets for anticancer drug have been identified including genes associated with cellcycle control and angiogenesis. Previously, an active and selective compound against WiDr fromTithonia diversifolia (Hemsley) A. has been isolated. The aim of this study was to evaluate theeffect of the isolated active compound fromT. diversifolia on the WiDr cell cycle and angiogenesis.Isolation of the active compound was performed by preparative thin layer chromatography (TLC)method. WiDr cell cycle was analyzed by flowcytometry using propidium iodide (PI).Antiangiogenesis effect was evaluated by immunocytochemistry method using anti-human VEGFmonoclonal antibody. The results showed that the effect of the isolated active compound onthe WiDr cell cycle depended on the concentration and the incubation time periods. Atconcentration of 4 μg/mL, it inhibited the WiDr cell cycle SubG1 phase after 36 and 48 hoursincubation and G1 phase after 72 hours incubation. While at concentration of 8 μg/mL, it clearlyinhibited the WiDr cell cycle G1 phase after 36, 48 and 72 hours incubation. Furthermore, theisolated active compound at concentration of 4 μg/mL significantly inhibited the VEGF expressionuntil 47.38% compared to control. In conclusion, the isolated active compound fromT. diversifoliainhibited cell cycle and angiogenesis of WiDr cell.

Cytotoxic activity of simvastatin in T47D breast cancer cell lines and its effect on cyclin D1 expression and apoptosis Bayu Putra; Mae Sri Hartati Wahyuningsih; Eti Nurwening Sholikhah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 49, No 2 (2017)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Background: Statins (HMG-CoA Inhibitors) is a drug used for decreasing plasma cholesterol levels and used in therapy to prevent coronary artery disease. Research in animals and epidemiological studies showed that statin therapy can decrease risk against cancer associated with cholesterol. Based on that result then research of cytotoxic activity against simvastatin knowing cultur T47D breast cancer cells and his influence in decreasing expression of cyclin D1 and induction of apoptosis has been done.Research objectives: The aims of this research is to prove activities of simvastatin against T47D breast cancer cell culture, especially to examine cytotoxic activity, cyclin D1 expression, and simvastatin effect in apoptotic induction.Research method: The type of this research is quasi experiment with using posttest with non-equivalent control group design. Cytotoxicity test performed on T47D breast cancer cell cultures using MTT assay to determine IC50 values after given simvastatine. Expression of cyclin D1 and apoptosis induction test detected using flow cytometry with antibody monoclonal anti-cyclin D1 and Annexin V-Pi, then analyzed by FACS-Calibur program.Results: Simvastatin has cytotoxic effect against T47D breast cancer cells with IC50 values 25.25 µg/mL. Simvastatin with concentrations of 6.31; 12.62; 25.25 and 50.5 µg/mL was able to decrease the cyclin D1 expression. Furthermore, simvastatin can induce apoptosis with EC50 values 26.96 µg/mL in T47D breast cancer cells.Conclusion: Simvastatin has cytotoxic activity of in T47D breast cancer cells and decreasing cyclin D1 expression and inducing apoptosis activity in T47D breast cancer cells.Keywords: simvastatin, cytotoxic, cyclin D1, apoptotic, T47D.

The effect of mitomycin-c in keloid fibroblast cultures Ishandono Dachlan; Teguh Aryandono; Mae Sri Hartati Wahyuningsih; Hardyanto Soebono; Yohanes Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 3 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACTKeloid occurs due to hyperactivity of keloid fibroblast (KF) in proliferation, migration, collagen deposition, together with low rates of collagen degradation. These are under the responsibility of TGF-b. Mitomycin C (MC) is used for treating keloid by a topical application during surgery at the level of 0.02% to 0.08%. Unfortunately, the lowest effective level of MC for keloid has not been determined yet. We aimed to determine the lowest effective level of MC in the suppression of KF activities. Various levels of MC diluted in growth medium were administered on KF that were isolated from six patients. After 24 hours and 72 hours of incubation, cellular proliferation, collagen deposition, cellular migration and level of TGF-b, were analyzed. Application of 120 uM MC on KF culture for 24 hours could significantly reduce TGF-b production from 1265.74 ± 274.81 pg/mL to 265.17 ± 12.20 pg/mL; proliferation index from 100% to 84.01 ± 12.91%; inhibit cellular migration to 64.38 ± 3.66%; but reduce collagen depositions from 100% to only 91.13 ± 10.19%. The lowest MC level is on 30 uM or equal with 0.001%. In conclusion, the lowest level of MC can suppress the activities of KF is 0.001%. Moreover, due to low activity in inhibiting collagen deposition, MC would be better as an adjuvant drug for keloid surgery.

Peppermint oil prevented oxidative stress in experimental animal – induced acute single bout of eccentric exercise (ASBEE): study on blood catalase and hydrogen peroxide (H2O2) and glucose transporter-4 (GLUT-4) expression on the muscle cells Dewi Aryanti; Denny Agustiningsih; Mae Sri Hartati Wahyuningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 3 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Peppermint oil is one of the essential oils with antioxidant activity that can reduce levels of reactive oxygen species (ROS). An acute single bout of eccentric exercise (ASBEE) is an acute exercise activity that can lead to increased ROS and cause skeletal muscle injury. This study aimed to assess the effect of peppermint oil in experimental animals induced with ASBEE with the purpose to measure catalase, hydrogen peroxide (H2O2) blood and glucose transporter-4 (GLUT-4) expression of skeletal muscle cells. A total of 30 Wistar rats (Rattus norvegicus) aged 20-24 weeks, weighing 160-350 g were divided into six groups i.e. T1 (n =5), T2 (n =4) and T3 (n =5) given peppermint oil orally at different dose of 0.25, 0.5 and 1.0 g/kg, respectively, one hour before inducing with ASBEE; C0 (n=5) not given peppermint oil and not induced with ASBEE; CA (n=5) not given peppermint oil and induced with ASBEE and CE (n=5) given vitamin E 400 mg/kg one h before induced with ASBEE. ASBEE induction was done by downhill running on a rat treadmill -50 with a load index of 70% VO2 max for 30 min. Twenty four h after induction of ASBEE, blood samples and muscle tissue were taken for examination of catalase, H2O2 and GLUT-4 expression. The results showed increased levels of blood catalase and decreased blood H2O2 levels in groups T1, T2, T3, and CE. The opposite occurred in the group CA. The GLUT-4 expression did not show any significant difference between groups. It was concluded that peppermint oil can improve the condition of oxidative stress caused by ASBEE.

Effect of tagitinin C isolated from Tithonia diversifoli (Hemsley) A Gray on migration activity and TGF-β1 levels on keloid fibroblast Elvira Santi; Mae Sri Hartati Wahyuningsih; Arief Budiyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 3 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Keloid is the formation of excessive scar tissue characterized by fibroblast hiperproliferations and collagen deposits that are similar with cancer cells. Tagitinin C is proven can inhibit proliferation and deposition of keloids collagen fibroblast. However, the mechanism of action of tagitinin C in migration activities and TGF-β1 levels of keloid fibroblasts has not been proved, yet. This study aimed to investigate the effects of tagitinin C isolated from Tithonia diversifoli (Hemsley) on migration activity and TGF-β1 expression of keloid fibroblast. This was quasi experimental study with post test only controlled group design using keloid fibroblasts isolated from keloid patients. The migration activity were performed by scratch assay and TGF-β1 levels were measured using an ELISA kits. Isolate tagitinin C was more active inhibit fibroblast keloid migration compare to the control groups (p<0.05) after 48 h incubation. TGF-β1 levels after incubation with isolate tagitinin C was lower then control group (p<0.05). In conclusion, isolate tagitinin C can inhibit migration and reduce TGF-β1 levels on keloid fibroblast

Sitotoksisitas rimpang temu mangga (Curcuma Mangga Val. & V. Zijp.) dan kunir putih ( Curcuma Zedoria i.) terhadap beberapa sel kanker manusia (in vitro) dengan metoda SRB Mae Sri Hartati Wahyuningsih; Sofia Mubarika; Bolhuis RLH; Nooter K; Oostrum RG
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 35, No 4 (2003)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Effect of tagitinin C isolated from kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] leaves on VEGF and TNF-α expressions ofkeloid fibroblast Arif Yusuf Wicaksana; Dwi Aris Nugrahaningsih; Mae Sri Hartati Wahyuningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 4 (2020)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Tagitinin C, an active constituent of Tithonia diversifolia (Hemsley) A. Gray, has been proven can inhibit the collagen deposition of keloid fibroblasts in vitro. However, its mechanism of action has not been widely studied. One possible mechanism involves growth factors and cytokines. Vascular endothelial growth factor (VEGF) and tumor necrosis factor alpha (TNF-α) play an important role in the collagen deposition. The study aimed to evaluate the effect of tagitinin C on VEGF and TNF-α expression in keloid fibroblasts culture. An experimental laboratory study using fibroblast cell lines at passages III and IV was performed. Treatments were divided into two groups i.e. the treatment groups after incubation with tagitinin C at various concentration of 1, 0.5, 0.25, and 0.125μg/ mL for 72 h, and the control group using culture media without tagitinin C. Following after incubation, the VEGF and TNF-α levels of keloid fibroblast culture supernatant were measured by ELISA. Kruskal-Wallis test continued using Mann-Whitney test or one way Anova continued by independent t test were applied to evaluate the differences between groups. A p value of less than 0.05 was considered statistically significant. The VEGF levels significantly decreases in concentration-dependent manner after treatment of the tagitinin C at various concentrations (p<0.05). However, no significantly difference in TNF-α levels was observed (p> 0.05). In conclusion, tagitinin C decreases the VEGF expression of keloid fibroblasts. However, it has no effect on the TNF-α expression.

SELEKTIVITAS EKSTRAK TERPURIFIKASI DAUN Tithonia diversifolia (Hemsley) A.Gray TERHADAP SEL HELA M. Sri Hartati Wahyuningsih; Rul Afiyah Syarif; Sri Suharmi; Tri Murini; Firandi Saputra; Adiguno Suryo W
Majalah Obat Tradisional Vol 18, No 1 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Tanaman kembang bulan [Tithonia diversifolia (Hemsley) A.Gray] merupakan salah satu tanaman yang digunakan dalam pengobatan tradisional untuk berbagai penyakit. Penelitian sebelumnya menyebutkan bahwa kembang bulan memiliki efek antiproliferasi pada sel kanker kolon (Col2). Ekstraksi dan partisi termonitor dengan uji aktivitas merupakan metode untuk mengambil senyawa aktif yang terkandung dalam ekstrak. Penelitian tentang selektivitas ekstrak terpurifikasi dari ekstrak aktif daun Kembang bulan terhadap sel HeLa belum pernah diteliti sebelumnya. Tujuan penelitian ini untuk mengetahui selektivitas ekstrak terpurifikasi daun Kembang Bulan pada sel HeLa dibandingkan dengan sel Vero dan menentukan nilai IC50 serta indeks selektivitasnya. Daun kembang bulan diekstraksi menggunakan pelarut Kloroform dan Metanol. Kedua ekstrak diuji efek sitotoksiknya pada sel Hela dengan beberapa konsentrasi (0,12 s/d 250μg/mL) menggunakan metode MTT (Mosmann, 1983). Pembacaan densitas optik dengan ELISA plate reader. Persentase kematian sel dihitung dan dianalisis dengan menggunakan regresi probit pada program SPSS 15 for Windows. Setelah diketahui nilai IC50 kedua ekstrak tersebut, dipilih salah satu yang memiliki IC50 lebih kecil kemudian dipurifikasi dengan Petroleum Eter (PE) dan diuji kembali pada sel HeLa, sari yang aktif diuji juga selektivitasnya dengan sel Vero, kemudian dihitung nilai IC50 nya. Nilai IC50 ekstrak metanol sebesar 1006,99μg/mL, ekstrak Kloroform sebesar 16,61μg/mL. Nilai IC50 sari larut PE sebesar 325,331μg/mL dan IC50 sari tidak larut PE sebesar 3,078μg/mL dan nilai IC50 pada sel vero adalah 80,30μg/mL. Nilai indeks selektivitas ekstrak terpurifikasi (sari tidak larut PE) adalah 26.09.

UJI SITOTOKSISITAS BATANG KAYU MEZZETIAPARVIFLORABECC. Mufidah Mufidah; Elly Wahyudin; Gatot S. Lawrence; M. Sri Hartati Wahyuningsih; Mirianti A. Manggau
Majalah Obat Tradisional Vol 18, No 1 (2013)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Aktivitas sitotoksik ekstrak larut aseton batang kayu MezzetiaparvifloraBecc. dievaluasi pada sel HeLa dan dibandingkan dengan sel normal vero untuk memastikan penggunaan sebagai tanaman obat tradisional untuk pengobatan tumor. Percobaan dengan sel normal vero menggunakan uji MTT menunjukkan persentase viabilitas sel sebanyak 96,8% pada konsentrasi 1000 ug dan tidak meningkat dengan meningkatnya konsentrasi ekstrak. Sedangkan pada percobaan dengan sel HeLa menunjukkan aktivitas sitotoksik yang rendah dengan persentase viabilitas sebesar 87,4% pada konsentrasi 1000μg/ml. Oleh karena itu ekstrak ini dikategorikan sebagai tidak beracun dan diperlukan studi selanjutnya untuk mengeksplorasi mekanisme yang bertanggung jawabterhadap efek antikanker dari tanaman tersebut.

Co-Authors Abdul Karim Zulkarnain Achmad Fudholi Adiguno Suryo W Adiguno Suryo W, Adiguno Suryo Akhmadi Akhmadi Alam, Gemini Amira Fawwaz Tsabitah Andina Setyawati, Andina Angga Anugerah Annisa Nurul Pratiwi Arfian Bela Mahardika Argo D, Imono Arief Budiyanto Arif Yusuf Wicaksana Arko Jatmiko Wicaksono, Arko Jatmiko Astuti, Puji Aurelia Priscilla Regita Putri Bayu Putra Beni Lestari Bolhuis RLH Christantie Effendy Damiana Sapta Candrasari Desyandri Desyandri Detty Siti Nurdiati Dewi Aryanti Dita Ayu Dewi Laras Sati Dwi Aris Agung Nugrahaningsih Dwi Aris Nugrahaningsih Dwi Sarbini Dwiki Yuliya Rahmawati Edy Meiyanto Elly Wahyudin Elly Wahyudin Elsi Dwi Hapsari Elvira Santi Emy Huriyati Emy Huriyati Eti Nurwening Sholikhah Farida Fitriyanti Farida Fitriyanti Firandi Saputra Firandi Saputra, Firandi Fitranto Arjadi Fudholi, Achmad - Gatot S. Lawrence Gatot S. Lawrence Hajid Rahmadianto Hajid Rahmadianto Mardihusodo Hajid Rahmadianto Mardihusodo, Hajid Rahmadianto Halimah, Wahyu Nur Hardyanto Soebono Hayati, Farida I Dewa Putu Pramantara Ibeneme, Sam Ibnu G Gandjar, Ibnu G Ibnu G. Gandjar Ibnu G. Gandjar Ibnu G. Ganjar, Ibnu G. Indwiani Astuti Indwiani Astuti Ishandono Dachlan Ita Fauzia Hanoum K. Nooter Ketut Shri Satya Wiwekananda KV Rao, KV Lisma Evareny, Mohammad Hakimi, Retna Siwi Padmawati M.Pd S.T. S.Pd. I Gde Wawan Sudatha . Mark T Hamann, Mark T Mark T. Hamann Mia Munawaroh Mirianti A. Manggau Mirianti A. Manggau, Mirianti A. Mufidah Mufidah Mufidah Mufidah Mustofa Mustofa Mustofa Mustofa Najiyati, Ifa Ngatidjan Ngatidjan Nia Krisniawati Nina Salamah Nooter K Nungki Anggorowati Nur Arfian Nur Arfian, Nur Nur Falah Setyawati Nyoman Kertia Oostrum RG Pinus Jumariyatno Prasetyawati, Citra Prasetyo Tri Kuncoro Pratiwi, Woro Rukmi Probosuseno Probosuseno R.L.M. Bolhuis Rakhmi Aulia Ranti, Imaniar Rita Rakhmawati, Rita Rul Afiyah Syarif S Sulastri Sadewa, Hamim Sari, Dinar Setyo Purwono Sofia Mubarika Haryana Sri Kadarsih Soejono Sri Peni Wastutiningsih Sri Suharmi, Sri Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Sugiyanto . Sugiyanto Sugiyanto Sulastri, S Sunarti Sunarti Susetyowati Tatsuo Takeya, Tatsuo Teguh Aryandono Tri Baskoro T.Satoto Tri Baskoro Tunggul Satoto Tri Murini Tri Murini Tri Murini Tri Murini Venny Vidayanti Wahyu Nur Halimah Wahyuono, Subagus Wahyuono, Subagus Wasis Pujiati Widiartini, Catharina Woro Rukmi Pratiwi Woro Rukmi Prtiwi Yacobus Christian Prasetyo Yohanes Widodo Wirohadidjojo Yulia Fauziyah Yuliani, Fara Silvia Yuniyanti, Mia Munawaroh

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