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All Journal Jurnal Fitopatologi Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Agrotrop : Journal on Agriculture Science SOCA: Jurnal Sosial Ekonomi Pertanian AGRITROP International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology Ecotrophic, Journal of Environmental Science E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Ilmu Lingkungan Cropsaver : Journal of Plant Protection Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA AGROMIX Abditani : Jurnal Pengabdian Masyarakat Jurnal Entomologi Indonesia Agro Bali: Agricultural Journal Agrica: Journal of Sustainable Dryland Agriculture Jurnal Bali Membangun Bali ABDI MOESTOPO: Jurnal Pengabdian pada Masyarakat Journal of Sustainable Development Science
Gusti Ngurah Alit Susanta Wirya
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Transportation Other

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POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFER TANAMAN LEGUMINOSAE UNTUK MENGENDALIKAN JAMUR Sclerotium rolfsii PENYEBAB PENYAKIT REBAH KECAMBAH PADA TANAMAN KEDELAI Komang Adi Mahartha; Dewa Ngurah Suprapta; Gusti Ngurah Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Damping off disease caused by Sclerotium rolfsii is an important disease of soybean, because soybean plant will die after attacked by pathogen. This pathogen can survive for a long time in the soil by sclerotia form. Some of rhizobacteria isolated from the rhizospheres of Leguminosae plants were known could control soil-borne pathogens. This study aims to test rhizobacteria isolated from the rhizosphere of Leguminosae plants that able to suppress the growth of S. rolfsii. Total of 11 isolates of 112 isolates rhizobacteria that could be isolated from Leguminosae plants had antifungal activity against S. rolfsii. Inhibition test of 11 isolates of rhizobacteria against S. rolfsii showed that three isolates had the highest ability to inhibit the growth of S. rolfsii namely isolates KtD1, KtD6, and KtB3. This result suggested that three isolates of rhizobacteria tested in this study can be further developed as bio-control agent to reduce the development of damping off disease on soybean.
PEMANFAATAN RHIZOBAKTERI PELARUT FOSFAT DARI TANAMAN LEGUM UNTUK PENINGKATAN PERTUMBUHAN DAN HASIL TANAMAN KEDELAI Ni Komang Budiyani; G. N. Alit Susanta Wirya; I Made Sudana; I Gusti Ngurah Raka
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Soybean is one of the strategic commodities in Indonesia. Soybean became the third most important crop after rice and maize. Leguminose plants are known to have microbial diversity in soils through their root exudates and can have a positive impact on plant growth. The use of rhizobacteria, known as Plant Growth Promoting Rhizobacteria (PGPR), is a bacteria that lives in the root area (rhizospher) and plays an important role in the growth of plants with the ability to form colonies around the roots quickly and can maintain environmental sustainability, one of bacteria rootsis phosphate solubilizing bacteria. This research is conducted to determine the influence of phosphate solubilizing bacteria on the growth and yield of soybean crops. This study used a factorial pattern (RAK) with 3 repetition and 9 treatments. Treatments of Rhizo 09 isolates from undis Rhizosfer here showed the best results.The analysis of sequencing and pyhlogeni shows that Rhizo 09 has close proximity withFlavobakterium johnsoniae from China and having a high similarity (98%) and a small genetic distance (0.003).
DETEKSI SIMULTAN CMV DAN CHIVMV PENYEBAB PENYAKIT MOSAIK PADA TANAMAN CABAI DENGAN DUPLEX RT-PCR I Gede Agus Adi Chandra; I Dewa Nyoman Nyana; I G N Alit Susanta Wirya; Gede Suastika
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Detect the presence virus causes mosaic symptoms by CMV and ChiVMV infecting chili pepper plants at the same time was conducted. There needs a study that aims to detect the associations of both viruses causes mosaic symptoms by single or double infection and simplify the detection process which is necessary for a long and expensive process. The used method is a duplex RT-PCR in hopes of simultaneously detecting two viruses causes mosaic disease. Based on the results of the study showed that the duplex RT-PCR method can detect double infection of CMV and ChiVMV on chili plants and this method more effective to detect two viruses simultaneously in a single PCR reaction.
ISOLASI DAN IDENTIFIKASI JAMUR PENGHASIL MIKOTOKSIN PADA BIJI KAKAO KERING YANG DIHASILKAN DI FLORES Emilia Simpllisiu Ake Wangge; Dewa Ngurah Suprapta; Gusti Ngurah Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

A study entitled “Isolation and Identification of Mycotoxin-producing Fungi on Cocoa Beans Produced in Flores” was done in order to know the population and species of fungi and to know the concentration of mycotoxin in the dried cocoa beans. The dry cocoa beans used in this study were collected from several locations in Flores. Isolation of the fungi was done using Sabouraud Dextrosa Agar (SDA), while the determination of mycotoxin concentration was done based on Enzym Linked Immunosorbent Assay (ELISA). Results of this study showed that,  there were several fungi were found in the dried cocoa beans produced in Flores, namely Aspergillus niger, A. flavus, A. parasiticus, A. fumigatusandMucor sp. All tested dried cocoa beans contained aflatoxin B1, where the highest aflatoxin B1 concentration was found in the fermented beans from West Manggarai with the concentration at 3.65 ppb, while the lowest concentration was 2.21ppb, which was found in the cocoa beans from Nagekeo. The highest concentration of ochratoxin A was 0.38  ppb, which was found in the cocoa beans from West Manggarai, while no ochratoxin A was detected in the cocoa beans from Ende.
APLIKASI CAMPURAN BIOURIN DENGAN AGEN PENGENDALI HAYATI UNTUK MENINGKATKAN PRODUKTIVITAS TANAMAN SAWI HIJAU (Brassica rapa Var. Parachinensis L.) I Gusti Agung Oka Hendrawati; I Made Sudana; G N Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The research was conducted in Pancasari Village, District Sukasada, Buleleng regency from July to November 2013. Purpose of this study was to determine the effectiveness of combination of bio urine with biological control agent in improving productivity and control pests and diseases in greens mustard plants. This study used a randomized block design (RBD) with one bio urine treatment factors, namely bio urine added crushed with Balinese spices (base genep), crushed mimba leaves, crushed leaves of the sour sop, chopped tobacco leaves, Bacillus thuringiensis, Trichoderma viride, Beauveria sp., bio urine without a mixture of biological control agents, chemical pesticides and is equipped with controls (just doused with water). The results of this study showed that bio urine with combination of the biological control agent variables significantly application of affect to plant height, leaf area index, chlorophyll quantity, above-ground fresh weight, fresh roots weight, above-ground dry weight, root dry weight, the percentage of club root, number of leaves damaged, the intensity of leaf damage and abundance imago of Liriomyza sp. Based on these results of this research it can be concluded that the treatment bio urine can control pest grasshoppers, control club root disease and improve productivity of greens mustard.  
BIOEKOLOGI Grapevine leafroll-Associated Virus type 3 (GLRAV-3) PENYEBAB PENYAKIT DAUN MENGGULUNG PADA TANAMAN ANGGUR (Vitis vinifera L.) Yuyun Fitriani; I Gede Rai Maya Temaja; G. N. Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 7 No 1 (2018)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The survey of vineyard fields in Buleleng, Bali, Indonesia was conducted, some grapevine cultivars were found had grapevine leaf roll disease symptomps. The symptoms including interveinal reddening or yelowing and fall leaves, down rolling of leaves, phloem disruption, and the fruit maturation is delayed. The analysis of enzyme linked immunosorbent assay (ELISA) of symptom leaf with Grapevine leaf roll-associated virus type 3 (GLRaV-3) antiserum shown the disease was corresponding to the GLRaV-3. The study was conducted to identify the mealybug that colonized of grapevine, to determine capability of mealybug to transmitted GLRaV-3 and host range GLRaV-3.Base on the survey ofmealybuginvineyard fields in Buleleng, Bali, the two types of mealybug were identified, namely Ferrisia virgata (Ckll.) (Hemiptera: Pseudococcidae) and Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae). Results of the transmission test showed that only M. Hirsutus are capable of transmitting GLRaV-3. The plant range host test of GLRaV-3 inoculation with M. hirsutuswas conducted and the symptom was detected on3plants species, grapevines (Vitis vinifera), native gooseberry (Physalis floridana) andshoe flower (Hibiscus rosa-sinensis) from18 species of plants that used.
MANFAAT BEBERAPA JENIS MIKROBA YANG DIISOLASI DARI KAYU LARU (Peltophorum pterocarpum.) DAN MUR SEBAGAI STARTER DALAM PEMBUATAN LARU DAN SOPI DI PULAU TIMOR Regina I. M BanoEt; I Made Sudana; I.G.N. Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Vol 5 No 1 (2016)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The traditional knowledge of farmers in producing alcohol from palm sap can be used as a basis to produce high-value economic products such as medical alcohol, industrial alcohol and ethanol. Food products and traditional fermented beverage are one of the main sources to obtain the potential microbe for microbial fermentation industry. Information on the wood of laru role as agents of microbial cultures and mur for a starter in the making of laru and sopi has not been much identified.The research was conducted in three stages. The first stage of the research, namely the microbial isolation of seven basic materials of making laru and sopi. The identification of microbes by using the API 20C AUX kits and a selection of microbes which have the ability to perform fermentation. The second stage of the research is the production test and gin laru utilize the selection and identification of microbes of, to find the best treatment combination that produces the highest alcohol content. The third stage of the research is the identification of molecular of microbes of culture results that have the best fermentation activities. The first and the third stages were exploratory studies by using the experimental design in the laboratory. The data were collected by means of direct observation. The theoretical analysis was made to describe the experimental results. The second stage of the testing of making laru and sopi to determine the percentage of alcohol content produced. The second stage was an experimental study, designed in the basic experimental design of RAL. The obtained data were analyzed with analysis of variance (ANOVA) to determine the real level of treatment effects, followed by LSD test to determine the level of significant differences between treatments. Each treatment combination was repeated four times. Two controls were used i.e; nira, palm sap without addition of a starter, and a solution of sugar with addition of the laru wood starter. The study was conducted in Labotory of Microbiology and Analytical Chemistry of The Food and Drug Administration Center for Kupang, East Nusa Tenggara.The experimental results showed that the microbes of laru wood and Mur are from the group of yeast. For the making of effective laru and sopi which to be sources of the microbes is red Mur and white Laru. The microbial species were identified as Saccharomyces cerevisiae. The resulting alcohol content on the microbes fermentor of Saccharomyces cerevisiae in the test of making red laru was 8.43%, white laru was 7.05%, sopi and nira/palm wine were 21.44%, and sopi from the aer sugar solution was 23.20%. Saccharomyces cerevisiae can also be combined with wood of laru as a starter in the making of laru and sopi. The combination of the two was the best treatment, resulted in the highest alcohol content at 8.90% of red laru, white laru at 8.40%, sopi from nira at 23.38%, and sopi from aer sugar solution at 23.91%.
POTENSI RIZOBAKTERI YANG DIISOLASI DARI RIZOSFIR TANAMAN GRAMINAE NON-PADI UNTUK MEMACU PERTUMBUHAN BIBIT PADI Ni Made Intan Maulina; Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya; Dewa Ngurah Suprapta
Journal of Agricultural Science and Biotechnology Vol 4 No 1 (2015)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Rice (Oriza sativa L.) is the main food for Indonesian people, however the rice production is not enough to fulfill all national demand. Rice productivity in Indonesia was about 4,5 ton/ha, relatively low when compared to other rice growing countries such Australia, Japan, and China. It is necessary to find a measure to increase the rice productivity in Indonesia. One of the Measure is the use of rhizobacteria isolated from the rhizozpheres of the plants belongs to the family Graminae. This study was done in order to find potential rhizobacteria from rhizospheres of five plant species belong to the family Graminae, namely Cymbopogon ciatrus, Imperata cylindrica, Saccharum officinarum, Pennisetum purpureum and Schizostachum mosum. Result of this study showed that 10 isolates of rhizobacteria obviously increased the number of lateral roots of rice seedling cultivar Ciherang. The rice seedling treated with isolate Al7Kla produced the highest number of lateral roots when compared to other isolates. This isolate may be used to promote the rice growth in order to increase the yield.
PEMANFAATAN PLANT GROWTH PROMOTING RHIZOBACTERIA UNTUK BIOSTIMULANTS DAN BIOPROTECTANTS Khamdan Khalimi; Gusti Ngurah Alit Susanta Wirya
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 4 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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Abstract

Various findings on the benefit of plant growth promoting rhizobacteria (PGPR) for agriculture have been reported by many research institutional. The enthusiasm to commercialize these bacteria as a promising alternative technology is triggered mainly by the to develop environmentally benign agriculture by reducing the use of synthetically agrochemical inputs (fertilizers and pesticides). These result suggested that application of PGPR could promoted the plant growth and increase the resistance of plant against fungi pathogen.
Uji Efektifitas Bakteri Antagonis Untuk Pengendalian Penyakit Layu pada Tanaman Stroberi SHAH KANIGARA ASADDIARI; GUSTI NNGURAH ALIT SUSANTA WIRYA; I KETUT SIADI; I MADE SUDANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.1, Januari 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Effectiveness Test of Antagonistic Bacteria to Control Strawberry Wilt Disease Strawberry (Fragaria sp.) Is a herbaceous fruit plant that was first discovered in Chile, USA. Bali Province is one of the provinces that produce strawberries inIndonesia. The development center for strawberry commodity in the Province of Baliis in the Bedugul area, that is in the Village of Pancasari, District of Sukasada andVillage of Candikuning, District of Tabanan. Since 2016, there have been quiteharmful disorders in strawberry plants in Bali due to wilting caused by the fungusFusarium oxysporum. Research on the effectiveness test of antagonistic bacteria tocontrol strawberry wilt disease was conducted in January 2019 to August 2019. Thepurpose of this study was to determine the ability of antagonistic bacteria to controlthe growth of wilt disease in strawberries in vivo. The method used is the isolation ofbacteria and pathogens, application of bacterial and pathogenic isolates, andobservation. The results of this study are that the P3 bacterial isolate is able to controlthe most effective pathogens with a disease percentage of 20 %.
Co-Authors Abd. Rasyid Syamsuri Agung, I Dewa Agung Putra Anak Agung Istri Kesumadewi ARIYANTA, I PUTU BAWA AStiningsih, Ana Agung Made Chiharu Hongo Damastra, Garda Bagus DEBBIE OKTAVIANI DEPARI Devi, Komang Saraswati Devi, Ni Luh Putu Hartika Sinta Devi, Putu Shinta Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Ngurah Suprapta Dharmadiatmika, I Made Agus Dinarkaya, Shah Mahapati DWI WIDANINGSIH Eka Wijayanti, Febri Emilia Simpllisiu Ake Wangge GARGITA, I WAYAN DIKSA Gede Mekse Korri Arisena Gede Suastika Gede Suastika GEDE WIJANA GREGORY C. LUTHER Gunadi, Gusti Alit GUSTI AYU DWITA ANDRAWINA H. Yuswanti Hartha , I Komang Gede Suweca HERRY KUSUMA YUDHA HESTIN YUSWANTI I Dewa Gede Raka Sarjana I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I G. R. M. TEMAJA I Gede Agus Adi Chandra I Gusti Agung Oka Hendrawati I GUSTI ALIT GUNADI I Gusti Alit Gunadi I GUSTI AYU ARI SANTIKADEWI I GUSTI AYU DEVI VALENIA SARI I GUSTI AYU KARISMAYATI I GUSTI NGURAH PRABU WIRA SANJAYA I GUSTI NGURAH RAKA I KADEK ARYARTHA I KETUT PURNA YASA I Ketut Siadi I KETUT SUMIARTHA I Komang Candra Giri Prayoga I Made Arimbawa I MADE DEDIK SETYADI I Made Sudana I MADE SUDANA I Made Sudana I Made Sudana I MADE SUDANA I MADE SUDARMA I MADE SUDARMA I MADE SUPARTHA UTAMA I MADE WINANTARA I NENGAH ARTHA I NYOMAN DARMA YASA I NYOMAN RAI I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I Putu Sudiarta I PUTU WIRYA SUPUTRA I Wayan Diara I WAYAN RUSMAN I Wayan Susila I.G.A. Gunadi Ida Ayu Putri Darmawati Ida Bagus Gde, pranatayana JOKO MARIYONO K.A. Yuliadhi K.B. Susrusa KESUMADEWI, ANAK AGUNG ISTRI KETUT AYU YULIADHI Ketut Ayu Yuliadhi KETUT BUDI SUSRUSA Khairun Nisak Syahdu KHAMDAN KALIMI Khamdan Khalimi Klett, Katrina KOMANG ADI MAHARTHA Komang Adi Mahartha Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi MADE MIKA MEGA ASTUTHI Made Satya Andrayuga Masahiro Shishido MEI NOVITA BR PARDEDE Mimi Sutrawati Muhammad Ikhsan Nulzaen N.N.A. Mayadewi NI KADEK NINA ARI SUCI NI KADEK SRI UTARI Ni Komang Budiyani Ni Luh Gde Sumardani NI LUH MADE INDAH MURDYANI DEWI Ni Luh Putu Citra Innosensia NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE INDRA PUSPAWATI Ni Made Intan Maulina Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE TRIGUNASIH NI NENGAH DARMIATI Ni Nengah Putri Adnyani NI NYOMAN ARI MAYADEWI NI NYOMAN DWI RESPITA NINGSIH Ni Putu Merthaningsih Ni Putu Pandawani NI PUTU RATIH SUDIARTINI NI WAYAN SUNITI Nyoman Bintang Kartika Sari Pradana, I Kadek Wira Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna Regina I. M BanoEt Retno Kawuri Rindang Dwiyani SANJAYA, I GUSTI NGURAH PRABU WIRA Sarjana, Dewa Gede Raka Selangga, Dewa Gede Wiryangga SHAH KANIGARA ASADDIARI SONIA ASHA HASARI SUGIARTA, DWI SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya TRI ASMIRA DAMAYANTI TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA UTAMA, I WAYAN EKA KARYA Wibisana, I Made Dicky Chandra WIDHIANTHINI WIDHIANTHINI Wigunanda, I Wayan Surya Aditya Wiraatmaja, Wayan Wulandari, Ni Kadek Pingkan Y. Fitriani YUDHA, I KADEK WISMA Yuliadhi , Ketut Ayu Yuyun Fitriani