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Isolasi dan Seleksi Bakteri Antagonis Sebagai Alternatif Pengendalian Penyakit Layu Stroberi I GUSTI NGURAH PRABU WIRA SANJAYA; GUSTI NGURAH ALIT SUSANTA WIRYA; TRISNA AGUNG PHABIOLA; I MADE WINANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.2, April 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Isolation and Selection of Antagonis Bacteria as an Alternative Control of Strawberry Wilt Diseases Strawberry (Fragaria sp.) is a plant that can produce fruit and a members of Rosaceae familia that entered Indonesia in the 1980s and began cultivativated in Candi Kuning Village, Bali at 1983. The farmer reported in 2017 at Candi Kuning Village, Bali, there are many strawberries plants are exposed to wilting disease caused by Fusarium oxysporum which can decreased strawberry production up to 80%. The purpose of this study is to find alternatives ways of chemical control that can cause environmental damage. Biological control, especially the use of antagonistic bacteria, has potential as a replacement because it is much more environmentally friendly and can create sustainable agriculture. The method used are isolation and selection, in vitro inhibitory test, and hypersensitive test on tobacco plants. Research activities included (1) sampling, (2) Fusarium oxysporum isolation from symptomatic strawberry plants, (3) isolation and selection of antagonistic bacterial candidates, (4) antagonistic candidate bacteria inhibitory test against Fusarium oxysporum by dual cultur method in vitro, and (5) hypersensitive test of antagonistic bacterial candidates on tobacco plant leaves. The result of this research is Fusarium oxysporum successfully isolated, obtained 24 candidates of antagonistic bacteria and only 4 candidates of antagonistic bacterial isolates capable of well inhibiting the development of Fusarium oxysporum with the average inhibitory percentage are more than 60 % which is the best inhibitory percentage of 90.17 % and in the hypersensitive test, the bacterial candidates tested were non-pathogenic to the plant.
Pengaruh Penggunaan Beberapa Paket Teknologi terhadap Perkembangan Penyakit Layu Fusarium pada Tanaman Cabai Rawit (Capsicum frutescens L.) dan Cabai Besar (Capsicum annuum L.) di Dataran Tinggi I WAYAN RUSMAN; NI WAYAN SUNITI; I KETUT SUMIARTHA; I PUTU SUDIARTA; GUSTI NGURAH ALIT SUSANTA WIRYA; I MADE SUPARTHA UTAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.3, Juli 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Influence of Some Packages of Technology to Fusarium Wilt Disease Development on Cayenne Chili Plants (Capsicum frutescens L.) and Long Chili (Capsicum annuum L.) in the HighlandsThe research was conducted in order to determine the influence and effectiveness of some technologies of Trichoderma sp. compost, rain shelter and pesticides to control the development of fusarium wilt disease on long chili and cayenne chili plants in the highlands. This research was done from September 2017 to February 2018, taking place in Pancasari Village, Buleleng Regency and in the Laboratory of Plant Disease, Udayana University. The study used a Factorial Randomized Block Design with two factors and replicated four times. The first factor was chili type, long chili and cayenne chili. The second factor was the use of technologies, Trichoderma sp. compost, rain shelter and pesticide. The variables observed in this research were symptoms and percentage of fusarium wilt disease. The results showed that all treatment technologies effectively suppressed disease development when compared to control treatment. Percentages of damage by fusarium wilt disease on Trichoderma sp., rain shelter, pesticide and control treatment were 22.50%, 23.75%, 27.50% and 45.00%, respectively.
Penggunaan Trichoderma sp.dan Penyambungan untuk Mengendalikan Penyakit Utama Tanaman Tomat (Licopersicum esculentum Mill.) di Desa Bangli, Kecamatan Baturiti, Tabanan I PUTU BAWA ARIYANTA; I PUTU SUDIARTA; DWI WIDANINGSIH; I KETUT SUMIARTHA; GUSTI ALIT SUSANTA WIRYA; MADE SUPARTHA UTAMA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.1, Januari 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Utilization of Trichoderma sp. and Grafting to Controlthe Mayor Diseases of Tomato (Licopersicum esculentum Mill.) in Bangli Village, Baturiti, Tabanan Control of plant diseases is one of the constraints in the cultivation of tomatoes. Control method performed by farmers generally use synthetic pesticides, however that cause environmental pollution. The use of Trichoderma sp. and grafting is an environmentally friendly technology in controlling plant diseases. The purpose of this study was in order to determine the ability of Trichoderma sp. and the grafting between the eggplant EG203 strain as rootstock and scions tomatoes as to control major diseases and improve tomato production. This study used a factorial randomized block design with six treatments and four replications.Diseases were found in field tomatoes are late blight (Phytophthora infestans) and yellow curly leaf disease (Tomato Yellow Leaf Curl Virus). Statistical analysis showed that Trichoderma sp. and grafting can reduce the disease severity of leaf blight and increase the production of tomato plants, but was unable to control the disease yellow leaf curl. Average of disease severity of leaf blight on grafting with screen and Trichoderma sp. was lower (61.11 and 62.03%) when compared to treatment without grafting and without Trichoderma sp. (82.99 and 75.47%). Average of yields on treatment grafting with screen and Trichoderma sp. was higher (3912.50 and 3822.22 g/plant) compared to treatment without grafting and without Trichoderma sp. (2858.33 and 3280.55 g/plant). Keywords: disease of tomato, Trichoderma sp., and grafting.
Efektifitas Pemberian Kompos Trichoderma Sp. Terhadap Pertumbuhan Tanaman Cabai (Capsicum Annum L.) I MADE DEDIK SETYADI; I NENGAH ARTHA; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Effectiveness composting Trichoderma sp. Against the Plant Growth Chilli (Capsicum annum L.)The objective of this study was to determine the effectiveness of compost Trichoderma sp. at the time of the nursery and moving the field on red pepper plants. This experiment was conducted February to April 2015, using a factorial randomized complete block design with three replications. The first factor seedling media (MT = Media soil + compost Trichoderma sp. With a volume ratio of 1: 1, MK = Media soil + compost Simantri the ratio of 1: 1, M = Media ground). The second factor in the media field (mt = Media soil + compost Trichoderma sp. With a volume ratio of 1: 1, mk = Media soil + compost Simantri the ratio of 1: 1, m = Media ground). The results showed that: there is interaction between media seedlings with the media on the field, the variables plant height and yield fruit of pepper plants in the field. The best treatment is shown by the media's treatment (MTmk) at high variable pepper is 61.67 cm and the media (MTmt) high were obtained are 60.78 cm. In the fruit of the pepper plant variables highest results in treatment (MTmt) with the amount of 916.67 g but not significantly different from the treatment (MKmt) with the results of 813.33 g.
Pengaruh Waktu Inokulasi Terhadap Laju Infeksi Penyakit Bean Common Mosaic Virus (BCMV) pada Tanaman Kacang Panjang (Vigna Sinensis L.) I GUSTI AYU KARISMAYATI; GUSTI NGURAH ALIT SUSANTA WIRYA; TRISNA AGUNG PHABIOLA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Effect of Inoculation Time towards the Infection Rate of Bean Common Mosaic Virus (BCMV) Disease on Long Beans (Vigna sinensis L.)Bean Common Mosaic Virus (BCMV) is kind of disease which greatly decrease the production of beans. The information about the spread of this disease is unknown. Therefore, it should be a research to be investigated. The purpose of the study is to determine the effect of inoculation time towards the infection rate of BCMV disease on long beans. The study uses a Randomized Complete Block Design (RCB) consisted of eight treatments and four replications, namely: the treatment of plants which originated from infected seeds of BCMV, inoculated at the age of 2, 3, 4, 5, 6, and 7 week after planting, and without inoculation as a control.The results showed that the younger of long beans infected by BCMV, resulting in the higher infection rate of disease and the symptoms caused would be more severe. The infection rate on plants which were inoculated at the age of 2, 3, 4, 5, 6, 7 week after planting and without inoculation were respectively 0,85; 0,77; 0,73; 0,49; 0,36; 0,31; and 0. The inoculation treatments at the age of 2, 3, and 4 week after planting were highly vulnerable to be infected by BCMV and caused the symptoms of severe mosaic, severe leaf malformations, and thickening of the bone leaves, whereas the inoculation treatments at the age of 5, 6, and 7 week after planting were resistant against BCMV disease with symptoms caused were moderate to mild, while the treatments without inoculation did not show any infection symptoms of BCMV.
Kajian Ketahanan Terhadap Penyakit Busuk Daun (Phytophthora Infestans) pada Beberapa Galur Tomat I NYOMAN DARMA YASA; I PUTU SUDIARTA; I GUSTI NGURAH ALIT SUSANTA WIRYA; KETUT SUMIARTHA; I MADE SUPARTHA UTAMA; GREGORY C. LUTHER; JOKO MARIYONO
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol. 1, No. 2, Oktober 2012
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Study of Resistance of Tomato Lines to Late Blight Disease (Phytophthora Infestans) Indonesia is one of the tropical countries with high potential of horticulture, especially tomato. To produce high quality and quantity of tomato the one problem is plant diseases. The most cereus disease was reported as late blight, it is caused by Phytophthora infestans. P. infestans is commonly out break in high land area, with more than 1000 m acl.  The late blight can reduce the production of tomato or in some case make the loss of yield. To control the disease the farmers have been used the chemical fungicide. However the chemical fungicides have many negative impacts. Therefore to control the disease the one of possible way is using resistant plant. On the other hand the tomato has been planted in Indonesia has not resistance by the late blight. Base on that, the experiment to select some resistant plant was conducted. The experiment was done in high land area in Bukitcatu and Pancasari (Bedugul area 1200 m acl).  The experiment was conducted used 16 lines and 4 varieties. The15 lines were imported from AVRDC and 1 line and 3 varieties were come from IVEGRI and 1 variety is local used by farmer. The result shown the most resistance lines are AVTO 0922, AVTO 1122, and AVTO 1143 base on the disease incidence and severity observation. The AVTO 0922, AVTO 1122, and AVTO 1143 were significant difference with another lines and varieties. Keywords: Tomato lines, Phytophthora infestans, resistant plant
Pemanfaatan Bacillus thuringiensis dan Kompos Trichoderma sp. untuk Mengendalikan Hama dan Penyakit Utama Tanaman Kubis (Brassica oleraceae L.) di Desa Bangli, Kecamatan Baturiti, Kabupaten Tabanan I KETUT PURNA YASA; GUSTI NGURAH ALIT SUSANTA WIRYA; MADE SUPARTHA UTAMA; I PUTU SUDIARTA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.2, April 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The utilization of Bacillus thuringiensis and Compost Plus Trichoderma sp to Control the Key Pests and Diseases of Cabbage (Brassica oleraceae) in Bangli Village Baturiti District, Tabanan Regency. The experimental design was used Factorial Randomize Complete Blok Design, with two factors: Factor I : (A) Seedling of Trichoderma sp., Soil containing compost and Trichoderma sp., (B) Seedling of Trichoderma sp., Soil containing compost but without Trichoderma sp., (C) Cow manure and spice extracts bali. Factor II : (a) B. thuringiensis and (b) Without B. thuringiensis. The results treatment of seedling with Trichoderma sp., Soil containing compost and Trichoderma sp. showed the lowest percentage of the disease (32.5%), and disease severity (19.37%) if compare with other treatments. On the order hand B. thuringiensis was effect to the population of C. binotalis and total of cabbage production. The population of C. binotalis was lower on cabbage with BT treatment (2,33) if compare without BT treatment (9,65 ). Treatment of seedling with Trichoderma sp., Soil containing compost and Trichoderma sp. showed the highest total yield (16 kg/plot), as well as the treatment of B. thuringiensis was highest total of yield (16.20 kg/plot).
Pemanfaatan Bakteri Azotobacter untuk Meningkatkan Efisiensi Penggunaan Pupuk Urea pada Tanaman Padi (Oryza sativa L.) NI MADE INDRA PUSPAWATI; KHAMDAN KHALIMI; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Utilization of Azotobacter Bacteria to Increase the Efficiency of Urea Fertilizer Use in Rice Plants (Oryza sativa L.) Rice is a food plant in the form of grasses (Gramineae) originating from the tropical and subtropical continents of Asia and West Africa. Nearly half of the world's population, including Indonesia, uses rice as the staple food consumed to meet food needs. Based on BPS data, the population shows an increase every five years. It is inversely proportional to the decreasing total rice production. One of the efforts to improve the cultivation technology package and increase the quality of intensification is the use of Azotobacter bacteria as a biofertilizer. Based on the isolation results, 52 Azotobacter isolates were found grown on Abhys mannitol agar. Based on the results of the selection of Azotobacter bacterial isolates, it was found that four Azotobacter bacterial isolates had the best effect on root growth of rice plants, namely the Azotobacter PD3, PD23, PD48, and PD51 bacterial isolates. The test results with GC-MS showed that 2 compounds were thought to contribute to increasing the growth of rice plants. These compounds were n-Hexadecanoic acid and oleic acid, which were included in the fatty acid group. The isolates of the Azotobacter PD3, PD48, PD51 bacteria were able to increase the efficiency of using urea by 25-50%. In comparison, the PD23 bacteria were able to increase the efficiency of using urea by 25% based on the number of tillers.
Penggunaan Trichoderma sp. yang Ditambahkan pada Berbagai Kompos untuk Pengendalian Penyakit Layu Tanaman Stroberi (Fragaria sp.) LUTFI SURYAWAN; GUSTI NGURAH ALIT SUSANTA WIRYA; I PUTU SUDIARTA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.4, Oktober 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The Usage of Trichoderma sp. That Added Into A Variety of Compost For The Control Of Wilt Desease In Strawberries (Fragaria Sp.) The research of utilizationTrichoderma sp. that added into a some kind of composts for the control of strawberries wilt desease had been done on December 2016 until May 2017. The purpose of this research is to find the best compost that combined with Trichoderma sp. for the control of wilt disease in strawberry. This research was carried out two stages that is in the laboratory to get the best antagonist microbes and in the greenhouse to get the best compost as an antagonistic microbial medium to controlling wilt disease in strawberries plants. The result of this research showed that the antagonistic microbial that has the highest percentage of inhibitory activity against the wilt disease pathogen was the antagonistic microbial from the rhizosphere of clove plants by 86 %. It was suspected that the antagonistic microbial is Trichoderma sp. Greenhouse research showed the lowest percentage of disease found in the compost treatment of antagonistic microbial + goat manure is 0%. The best compost as the carrier of the antagonistic microbial (Trichoderma sp.) is the compost that made from goats manure.
Metode Grafting Tanaman Tomat Menggunakan Batang Bawah Terong Untuk Mengatasi Genangan dan Mengendalikan Penyakit Tular Tanah di Kebun Percobaan Fakultas Pertanian Universitas Udayana I PUTU SUDIARTA; GUSTI NGURAH ALIT SUSANTA WIRYA; I MADE WINANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.1, Januari 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Grafting Method of Tomato using rootstock of eggplant to minimize problems caused by flooding and soil-borne diseases in Faculty of Agriculture Research Field Udayana University The study was conducted to determine the effectiveness of the Grafting Method of Tomato using rootstock of eggplant to minimize problems caused by flooding and soilborne diseases in Faculty of Agriculture Research Field Udayana University. The experimental design was used a Randomize bloc design (RBD), with 3 treatments including control and 9 replications. The treatments are, grafting, non-grafting and control usually use by the farmers. The first step of the research is grafting or tomato as a scion with eggplant as a rootstock. The grafting method was used is base one the AVRDC-method. The result showed the grafted tomato was free from wilting disease on the other hand the non-grafted tomato showed the symptom of wild diseases, the data indicated the grafted tomato was resistant by the soil-borne diseases including bacteria wilt as well as Fusarium wilt disease. For flooding we cannot compare the significantly result between grafted tomato and non-grafted tomato because the season is not wet season. The suggestion of this research is recommended to doing the research in wet season for more clear understand the effect of grafted tomato to the flooding.
Co-Authors Abd. Rasyid Syamsuri Agung, I Dewa Agung Putra Anak Agung Istri Kesumadewi ARIYANTA, I PUTU BAWA AStiningsih, Ana Agung Made Chiharu Hongo Damastra, Garda Bagus DEBBIE OKTAVIANI DEPARI Devi, Komang Saraswati Devi, Ni Luh Putu Hartika Sinta Devi, Putu Shinta Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Ngurah Suprapta Dharmadiatmika, I Made Agus Dinarkaya, Shah Mahapati DWI WIDANINGSIH Eka Wijayanti, Febri Emilia Simpllisiu Ake Wangge GARGITA, I WAYAN DIKSA Gede Mekse Korri Arisena Gede Suastika Gede Suastika GEDE WIJANA GREGORY C. LUTHER Gunadi, Gusti Alit GUSTI AYU DWITA ANDRAWINA H. Yuswanti Hartha , I Komang Gede Suweca HERRY KUSUMA YUDHA HESTIN YUSWANTI I Dewa Gede Raka Sarjana I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I G. R. M. TEMAJA I Gede Agus Adi Chandra I Gusti Agung Oka Hendrawati I Gusti Alit Gunadi I GUSTI ALIT GUNADI I GUSTI AYU ARI SANTIKADEWI I GUSTI AYU DEVI VALENIA SARI I GUSTI AYU KARISMAYATI I GUSTI NGURAH PRABU WIRA SANJAYA I GUSTI NGURAH RAKA I KADEK ARYARTHA I KETUT PURNA YASA I Ketut Siadi I KETUT SUMIARTHA I Komang Candra Giri Prayoga I Made Arimbawa I MADE DEDIK SETYADI I MADE SUDANA I Made Sudana I MADE SUDANA I Made Sudana I Made Sudana I MADE SUDARMA I MADE SUDARMA I MADE SUPARTHA UTAMA I MADE WINANTARA I NENGAH ARTHA I NYOMAN DARMA YASA I NYOMAN RAI I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I Putu Sudiarta I PUTU WIRYA SUPUTRA I Wayan Diara I WAYAN RUSMAN I Wayan Susila I.G.A. Gunadi Ida Ayu Putri Darmawati Ida Bagus Gde, pranatayana JOKO MARIYONO K.A. Yuliadhi K.B. Susrusa KESUMADEWI, ANAK AGUNG ISTRI KETUT AYU YULIADHI Ketut Ayu Yuliadhi KETUT BUDI SUSRUSA Khairun Nisak Syahdu KHAMDAN KALIMI Khamdan Khalimi Klett, Katrina KOMANG ADI MAHARTHA Komang Adi Mahartha Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi MADE MIKA MEGA ASTUTHI Made Satya Andrayuga Masahiro Shishido MEI NOVITA BR PARDEDE Mimi Sutrawati Muhammad Ikhsan Nulzaen N.N.A. Mayadewi NI KADEK NINA ARI SUCI NI KADEK SRI UTARI Ni Komang Budiyani Ni Luh Gde Sumardani NI LUH MADE INDAH MURDYANI DEWI Ni Luh Putu Citra Innosensia NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE INDRA PUSPAWATI Ni Made Intan Maulina Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE TRIGUNASIH NI NENGAH DARMIATI Ni Nengah Putri Adnyani NI NYOMAN ARI MAYADEWI NI NYOMAN DWI RESPITA NINGSIH Ni Putu Merthaningsih Ni Putu Pandawani NI PUTU RATIH SUDIARTINI NI WAYAN SUNITI Nyoman Bintang Kartika Sari Pradana, I Kadek Wira Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna Regina I. M BanoEt Retno Kawuri Rindang Dwiyani SANJAYA, I GUSTI NGURAH PRABU WIRA Sarjana, Dewa Gede Raka Selangga, Dewa Gede Wiryangga SHAH KANIGARA ASADDIARI SONIA ASHA HASARI SUGIARTA, DWI SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya TRI ASMIRA DAMAYANTI TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA UTAMA, I WAYAN EKA KARYA Wibisana, I Made Dicky Chandra WIDHIANTHINI WIDHIANTHINI Wigunanda, I Wayan Surya Aditya Wiraatmaja, Wayan Wulandari, Ni Kadek Pingkan Y. Fitriani YUDHA, I KADEK WISMA Yuliadhi , Ketut Ayu Yuyun Fitriani